Rorug05G0314600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004872
Physical Location & Seq
Reverse (-)
37320785 .. 37322205
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0314600.1

Sequence Viewer

Length: 1248 bp
ATGACGGAAAACCAACTACCGGAAGACATCATAGTCTCCGACATCCTGGTCAAACTCCCCATCAAATCCTTAATCCGCTGCACTTGCGTCTCAAAACGGTGGCGTTTCATAATCTCCGACCCCGAATTCGCCAAATCTCACTTCCAACAAGCTCGCCGCCACCACACCTTGACCCGCCGCCTCCTCCTCTACTTCTCCCCCTCCCAAGTCGAATCCGTTGACTTGGAAACGCCGCCGTCTTCGCCTTCTCCGATCCGCAACCTCACCTGTAAACTGAACAACCAACCCGAATCGATCAAGCTCCTCGGCTCCTGCAACGGCCTGGTATGCGTCTGTACGGATTCTACTTTAAGCAGTTGTGATATTTTCATCTGGAACCCATCAACTGGGTTCTTGCGTAATCTACCGTACCCGTGTTTTTCCTCGGCAACACACACCGTTCTCTACGGCGGGTTCGGTTACTTGTCGGCCACGGACGACTACAAGGTCGTGGTGGCGGCGGACAATGTGTTCGACTACGGCGATAATGTGGTGGAGGTGAAGGTGTTGTCATGGAGAACAGGGGTTTGGAGAAAGATTGAAGTGCACCCTTTGTTGTCTTACACGTTGTACCCGGCGGCGGTTGTGGTGGGGGAGACGCTTCATTGGTTTCTGAACGATCGTTGGTTAGGGTCGACGATTATTGGTTTTGATTTGGACAAGGAGGAGTTTAGAGAGATGCCGCCGCCGCCGCCGAGTTTTTGGCATAGATATGATAGAGATTATGTTGGGTGTGTGGGTTGTTGGGGAGGAAGGCTACATGTGTTTCGATATACGGCTCGTGTTTGTGATGTTATGGAGTTATGGGTTAGGGGTGAGAATGGTGATGGTGAGTCGTGGACCGAGGTGTTCAAGTTGAAAATAACCAATGAGCCTCGGAAGGCTGTGTTTCTGCGGCCGTTTTTGGCTGTGGGAAGTAGTGCAGTTGTGGCGAAAGTGACTAGGAACTCGACGATGGAGATGGTCAGGATTGAACCTAGAGATGAGGAACCGGTTGTAGATATGTTTAAGAGGGGGGTTGAACCTGCAATGATTCTATATGAAGAGAGCCTGCACCGGCTTCCTGGTTGTGGTGCGGTGGAAGAAGAGAAGGTGGAAGAGATTCAAACCCCAATATTTGGTGCTTTATTCTGGATTACTGATCACTGGTACTCTATTCTGGCTTTGGTTGGTTTTCTTATTTCTCTGTGCTATGTACCTGTAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

415

Amino Acids

47.31

Weight (kDa)

5.86

Isoelectric Point (pI)

46.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 5 - 49 1.3e-07 F-box-like
F-box PF00646 5 - 43 1.5e-07 F-box domain
FBA_1 PF07734 73 - 309 1.5e-20 F-box associated beta propeller domain
FBA_3 PF08268 84 - 296 6e-14 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 97 - 234 8.9e-07 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 32, 47, 485
Acc36I ACCTGC 1 cut(s) 1072
AccB7I CCANNNNNTGG 2 cut(s) 386, 1157
AccI GTMKAC 1 cut(s) 674
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 2 cut(s) 468, 935
AcsI RAATTY 1 cut(s) 125
AfaI GTAC 5 cut(s) 337, 410, 611, 1190, 1236
AfiI CCNNNNNNNGG 5 cut(s) 19, 386, 619, 1109, 1157
AflIII ACRYGT 2 cut(s) 603, 799
AgeI ACCGGT 1 cut(s) 1030
AgsI TTSAA 6 cut(s) 581, 892, 898, 1013, 1061, 1145
AjnI CCWGG 3 cut(s) 45, 321, 1102
AjuI GAANNNNNNNTTGG 4 cut(s) 125, 157, 550, 582
AloI GAACNNNNNNTCC 2 cut(s) 494, 526
AluBI AGCT 2 cut(s) 152, 301
AluI AGCT 2 cut(s) 152, 301
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 3 cut(s) 40, 94, 629
Alw44I GTGCAC 1 cut(s) 584
AlwI GGATC 1 cut(s) 247
AoxI GGCC 3 cut(s) 319, 468, 935
ApaLI GTGCAC 1 cut(s) 584
ApeKI GCWGC 1 cut(s) 78
ApoI RAATTY 1 cut(s) 125
ArsI GACNNNNNNTTYG 4 cut(s) 110, 142, 494, 526
AsiGI ACCGGT 1 cut(s) 1030
Asp700I GAANNNNTTC 1 cut(s) 1140
AspS9I GGNCC 1 cut(s) 879
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 5 cut(s) 256, 550, 866, 875, 881
AvaII GGWCC 1 cut(s) 879
BaeGI GKGCMC 1 cut(s) 588
BauI CACGAG 1 cut(s) 819
BbsI GAAGAC 2 cut(s) 30, 231
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 65
BccI CCATC 5 cut(s) 68, 388, 860, 988, 994
BceAI ACGGC 6 cut(s) 220, 334, 463, 535, 831, 922
BciT130I CCWGG 3 cut(s) 47, 323, 1104
BclI TGATCA 1 cut(s) 1180
BcnI CCSGG 1 cut(s) 614
BcoDI GTCTC 3 cut(s) 40, 94, 629
BfaI CTAG 2 cut(s) 981, 1017
BfuAI ACCTGC 1 cut(s) 1072
Bme1390I CCNGG 4 cut(s) 47, 323, 614, 1104
Bme18I GGWCC 1 cut(s) 879
BmgT120I GGNCC 1 cut(s) 879
BmiI GGNNCC 3 cut(s) 310, 377, 1029
BmrFI CCNGG 4 cut(s) 47, 323, 614, 1104
BmrI ACTGGG 1 cut(s) 396
BmsI GCATC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 396
BpiI GAAGAC 2 cut(s) 30, 231
BpuMI CCSGG 1 cut(s) 614
Bsa29I ATCGAT 1 cut(s) 293
BsaJI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BsaWI WCCGGW 2 cut(s) 19, 1030
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 386, 619, 1109, 1157
Bse118I RCCGGY 2 cut(s) 1030, 1095
Bse1I ACTGG 2 cut(s) 391, 1190
Bse3DI GCAATG 1 cut(s) 1074
BseBI CCWGG 3 cut(s) 47, 323, 1104
BseCI ATCGAT 1 cut(s) 293
BseDI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BseGI GGATG 1 cut(s) 42
BseLI CCNNNNNNNGG 5 cut(s) 19, 386, 619, 1109, 1157
BseMI GCAATG 1 cut(s) 1074
BseNI ACTGG 2 cut(s) 391, 1190
BseRI GAGGAG 4 cut(s) 173, 176, 293, 719
BseSI GKGCMC 1 cut(s) 588
BseX3I CGGCCG 1 cut(s) 935
BseXI GCAGC 1 cut(s) 65
BsgI GTGCAG 3 cut(s) 64, 981, 1076
Bsh1285I CGRYCG 2 cut(s) 661, 938
BshFI GGCC 3 cut(s) 321, 470, 937
BshTI ACCGGT 1 cut(s) 1030
BshVI ATCGAT 1 cut(s) 293
BsiEI CGRYCG 2 cut(s) 661, 938
BsiHKAI GWGCWC 1 cut(s) 588
BsiSI CCGG 4 cut(s) 20, 614, 1031, 1096
BslI CCNNNNNNNGG 5 cut(s) 19, 386, 619, 1109, 1157
BsmAI GTCTC 3 cut(s) 40, 94, 629
BsmBI CGTCTC 2 cut(s) 94, 629
BsnI GGCC 3 cut(s) 321, 470, 937
Bsp1286I GDGCHC 1 cut(s) 588
Bsp143I GATC 4 cut(s) 252, 294, 658, 1180
BspANI GGCC 3 cut(s) 321, 470, 937
BspDI ATCGAT 1 cut(s) 293
BspLI GGNNCC 3 cut(s) 310, 377, 1029
BspMI ACCTGC 1 cut(s) 1072
BspPI GGATC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 1074
BsrFI RCCGGY 2 cut(s) 1030, 1095
BsrI ACTGG 2 cut(s) 391, 1190
BssAI RCCGGY 2 cut(s) 1030, 1095
BssECI CCNNGG 5 cut(s) 304, 423, 471, 882, 914
BssMI GATC 4 cut(s) 252, 294, 658, 1180
BssSI CACGAG 1 cut(s) 819
Bst2BI CACGAG 1 cut(s) 819
Bst2UI CCWGG 3 cut(s) 47, 323, 1104
Bst4CI ACNGT 3 cut(s) 99, 408, 439
Bst6I CTCTTC 3 cut(s) 1077, 1119, 1131
BstC8I GCNNGC 2 cut(s) 154, 1091
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 42
BstKTI GATC 4 cut(s) 255, 297, 661, 1183
BstMAI GTCTC 3 cut(s) 40, 94, 629
BstMBI GATC 4 cut(s) 252, 294, 658, 1180
BstMCI CGRYCG 2 cut(s) 661, 938
BstMWI GCNNNNNNNGC 6 cut(s) 84, 241, 327, 727, 730, 968
BstNI CCWGG 3 cut(s) 47, 323, 1104
BstNSI RCATGY 1 cut(s) 803
BstSCI CCNGG 4 cut(s) 45, 321, 612, 1102
BstSLI GKGCMC 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 65
BstV2I GAAGAC 2 cut(s) 30, 231
BstZI CGGCCG 1 cut(s) 935
Bsu15I ATCGAT 1 cut(s) 293
BsuRI GGCC 3 cut(s) 321, 470, 937
BsuTUI ATCGAT 1 cut(s) 293
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 1183
BveI ACCTGC 1 cut(s) 1072
Cac8I GCNNGC 2 cut(s) 154, 1091
Cfr10I RCCGGY 2 cut(s) 1030, 1095
Cfr13I GGNCC 1 cut(s) 879
ClaI ATCGAT 1 cut(s) 293
CseI GACGC 3 cut(s) 76, 319, 646
Csp6I GTAC 5 cut(s) 336, 409, 610, 1189, 1235
CspAI ACCGGT 1 cut(s) 1030
CviAII CATG 2 cut(s) 552, 800
CviQI GTAC 5 cut(s) 336, 409, 610, 1189, 1235
DpnI GATC 4 cut(s) 254, 296, 660, 1182
DpnII GATC 4 cut(s) 252, 294, 658, 1180
DrdI GACNNNNNNGTC 3 cut(s) 32, 47, 485
DseDI GACNNNNNNGTC 3 cut(s) 32, 47, 485
EaeI YGGCCR 2 cut(s) 468, 935
EagI CGGCCG 1 cut(s) 935
Eam1104I CTCTTC 3 cut(s) 1077, 1119, 1131
EarI CTCTTC 3 cut(s) 1077, 1119, 1131
EciI GGCGGA 1 cut(s) 515
EclXI CGGCCG 1 cut(s) 935
Eco47I GGWCC 1 cut(s) 879
Eco52I CGGCCG 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 125
EcoRII CCWGG 3 cut(s) 45, 321, 1102
Esp3I CGTCTC 2 cut(s) 94, 629
FaeI CATG 2 cut(s) 555, 803
FatI CATG 2 cut(s) 551, 799
FauI CCCGC 2 cut(s) 182, 443
FbaI TGATCA 1 cut(s) 1180
FblI GTMKAC 1 cut(s) 674
FokI GGATG 1 cut(s) 29
FspBI CTAG 2 cut(s) 981, 1017
HaeIII GGCC 3 cut(s) 321, 470, 937
HapII CCGG 4 cut(s) 20, 614, 1031, 1096
HgaI GACGC 3 cut(s) 76, 319, 646
Hin1II CATG 2 cut(s) 555, 803
HincII GTYRAC 2 cut(s) 220, 675
HindII GTYRAC 2 cut(s) 220, 675
HinfI GANTC 6 cut(s) 212, 290, 341, 872, 1072, 1141
HpaII CCGG 4 cut(s) 20, 614, 1031, 1096
HphI GGTGA 5 cut(s) 256, 550, 866, 875, 881
Hpy166II GTNNAC 5 cut(s) 220, 272, 586, 675, 879
Hpy188I TCNGA 5 cut(s) 40, 118, 252, 654, 918
Hpy188III TCNNGA 3 cut(s) 373, 1006, 1171
Hpy8I GTNNAC 5 cut(s) 220, 272, 586, 675, 879
Hpy99I CGWCG 2 cut(s) 679, 994
HpyAV CCTTC 5 cut(s) 255, 535, 786, 913, 1123
HpyCH4III ACNGT 3 cut(s) 99, 408, 439
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 6 cut(s) 81, 315, 586, 962, 1067, 1093
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 241, 327, 727, 730, 968
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 2 cut(s) 555, 803
Ksp22I TGATCA 1 cut(s) 1180
Kzo9I GATC 4 cut(s) 252, 294, 658, 1180
LmnI GCTCC 2 cut(s) 306, 314
Lsp1109I GCAGC 1 cut(s) 65
LweI GCATC 1 cut(s) 708
MaeI CTAG 2 cut(s) 981, 1017
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 2 cut(s) 458, 976
MalI GATC 4 cut(s) 254, 296, 660, 1182
MboI GATC 4 cut(s) 252, 294, 658, 1180
MboII GAAGA 6 cut(s) 35, 231, 1094, 1133, 1136, 1148
MhlI GDGCHC 1 cut(s) 588
MluCI AATT 1 cut(s) 125
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 3 cut(s) 63, 141, 169
MroXI GAANNNNTTC 1 cut(s) 1140
MseI TTAA 3 cut(s) 71, 350, 1047
MslI CAYNNNNRTG 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 4 cut(s) 20, 614, 1031, 1096
MspR9I CCNGG 4 cut(s) 47, 323, 614, 1104
MvaI CCWGG 3 cut(s) 47, 323, 1104
MwoI GCNNNNNNNGC 6 cut(s) 84, 241, 327, 727, 730, 968
NciI CCSGG 1 cut(s) 614
NdeII GATC 4 cut(s) 252, 294, 658, 1180
NlaIII CATG 2 cut(s) 555, 803
NlaIV GGNNCC 3 cut(s) 310, 377, 1029
NmeAIII GCCGAG 3 cut(s) 285, 404, 759
NmuCI GTSAC 1 cut(s) 976
NspI RCATGY 1 cut(s) 803
PciI ACATGT 1 cut(s) 799
PcsI WCGNNNNNNNCGW 2 cut(s) 248, 519
PdmI GAANNNNTTC 1 cut(s) 1140
PfeI GAWTC 5 cut(s) 212, 290, 341, 1072, 1141
PflMI CCANNNNNTGG 2 cut(s) 386, 1157
PinAI ACCGGT 1 cut(s) 1030
Ple19I CGATCG 1 cut(s) 661
PleI GAGTC 1 cut(s) 880
PpsI GAGTC 1 cut(s) 880
PscI ACATGT 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 45, 321, 1102
PspGI CCWGG 3 cut(s) 45, 321, 1102
PspN4I GGNNCC 3 cut(s) 310, 377, 1029
PspPI GGNCC 1 cut(s) 879
PvuI CGATCG 1 cut(s) 661
RsaI GTAC 5 cut(s) 337, 410, 611, 1190, 1236
RsaNI GTAC 5 cut(s) 336, 409, 610, 1189, 1235
RseI CAYNNNNRTG 1 cut(s) 750
SalI GTCGAC 1 cut(s) 673
SaqAI TTAA 3 cut(s) 71, 350, 1047
Sau3AI GATC 4 cut(s) 252, 294, 658, 1180
Sau96I GGNCC 1 cut(s) 879
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 4 cut(s) 47, 323, 614, 1104
SduI GDGCHC 1 cut(s) 588
SfaNI GCATC 1 cut(s) 708
SinI GGWCC 1 cut(s) 879
SmiMI CAYNNNNRTG 1 cut(s) 750
Sse9I AATT 1 cut(s) 125
SspI AATATT 1 cut(s) 1155
SspMI CTAG 2 cut(s) 981, 1017
StyD4I CCNGG 4 cut(s) 45, 321, 612, 1102
TaaI ACNGT 3 cut(s) 99, 408, 439
TaiI ACGT 1 cut(s) 608
TaqI TCGA 6 cut(s) 210, 293, 513, 674, 808, 989
TaqII GACCGA 1 cut(s) 896
TasI AATT 1 cut(s) 125
TfiI GAWTC 5 cut(s) 212, 290, 341, 1072, 1141
Tru1I TTAA 3 cut(s) 71, 350, 1047
Tru9I TTAA 3 cut(s) 71, 350, 1047
TscAI CASTG 1 cut(s) 1190
TseFI GTSAC 1 cut(s) 976
TseI GCWGC 1 cut(s) 78
Tsp45I GTSAC 1 cut(s) 976
TspDTI ATGAA 4 cut(s) 97, 358, 632, 1095
TspGWI ACGGA 4 cut(s) 20, 205, 353, 488
TspRI CASTG 1 cut(s) 1190
Van91I CCANNNNNTGG 2 cut(s) 386, 1157
VneI GTGCAC 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 879
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 803
XmiI GTMKAC 1 cut(s) 674
XmnI GAANNNNTTC 1 cut(s) 1140
XspI CTAG 2 cut(s) 981, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.