RLG00000002958

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0004886
Physical Location & Seq
Reverse (-)
42265870 .. 42267275
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002958

Sequence Viewer

Length: 459 bp
ATGTTGCCATCACCGCGCGCCGCAAGTTCGAGGGTCCATGCCAGCGACAGAGACGAAGAGCTCACTCTATTTCTCAAGATGCGGCGATGCGACAAAGACAAGGAGAAAACTAGAACTTTCTGCTACTTCCAAGCAAGAACCGCGGAGAGCTCGATGCTGCTCCTTCGCTTGCTACGACGGCGTTTTGGAGATTCCCGGTTGCTGGGACTATTTTCACCTCGATTCCCAAGCCAGAGCTTTGGGAAGAGGGACTTCGTCGGCTTGGTGGTAGAGAACCTGAAAATCAATGGGGCTTCGGCGATTTTGGACTTCGGGAACAAGATAGGGCAAACTGAGGCCTATTTCGCAGCCTTCTTGGATGGGATGTCGCGCCAGTTCTTCAGGAGCTTGTCGGTGCCTTTTCGGCACGATGAGAATGGTATCATGGCCGTTTGGTTTGATTTGGATAAGGTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.5

Weight (kDa)

9.85

Isoelectric Point (pI)

60.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 394
AccII CGCG 4 cut(s) 16, 18, 143, 370
AciI CCGC 5 cut(s) 14, 21, 82, 141, 143
AcoI YGGCCR 1 cut(s) 426
AcuI CTGAAG 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 202
AluBI AGCT 4 cut(s) 61, 150, 237, 387
AluI AGCT 4 cut(s) 61, 150, 237, 387
Alw21I GWGCWC 2 cut(s) 63, 152
Alw26I GTCTC 1 cut(s) 45
AoxI GGCC 2 cut(s) 336, 426
ApeKI GCWGC 2 cut(s) 157, 347
AspLEI GCGC 3 cut(s) 18, 20, 372
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 3, 207
AvaII GGWCC 1 cut(s) 34
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 63, 152
Bbv12I GWGCWC 2 cut(s) 63, 152
BbvI GCAGC 2 cut(s) 144, 359
BccI CCATC 2 cut(s) 16, 353
BceAI ACGGC 2 cut(s) 194, 413
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 1 cut(s) 111
BglI GCCNNNNNGGC 1 cut(s) 403
BisI GCNGC 4 cut(s) 21, 83, 158, 348
BlsI GCNGC 4 cut(s) 22, 84, 159, 349
Bme1390I CCNGG 1 cut(s) 196
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 2 cut(s) 35, 396
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 3 cut(s) 69, 77, 144
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 141
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 2 cut(s) 364, 369
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 373
BsePI GCGCGC 1 cut(s) 16
BseXI GCAGC 2 cut(s) 144, 359
BseYI CCCAGC 1 cut(s) 202
Bsh1236I CGCG 4 cut(s) 16, 18, 143, 370
BshFI GGCC 2 cut(s) 338, 428
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 63, 152
BsiSI CCGG 1 cut(s) 196
BslFI GGGAC 2 cut(s) 219, 263
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 2 cut(s) 219, 263
BsnI GGCC 2 cut(s) 338, 428
Bsp1286I GDGCHC 2 cut(s) 63, 152
BspACI CCGC 5 cut(s) 14, 21, 82, 141, 143
BspANI GGCC 2 cut(s) 338, 428
BspCNI CTCAG 1 cut(s) 325
BspFNI CGCG 4 cut(s) 16, 18, 143, 370
BspLI GGNNCC 2 cut(s) 35, 396
BspQI GCTCTTC 1 cut(s) 51
BspT107I GGYRCC 1 cut(s) 394
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 141
BssHII GCGCGC 1 cut(s) 16
Bst6I CTCTTC 2 cut(s) 51, 239
BstC8I GCNNGC 3 cut(s) 18, 43, 170
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 364, 369
BstFNI CGCG 4 cut(s) 16, 18, 143, 370
BstHHI GCGC 3 cut(s) 18, 20, 372
BstMAI GTCTC 1 cut(s) 45
BstMWI GCNNNNNNNGC 5 cut(s) 13, 140, 178, 344, 403
BstSCI CCNGG 1 cut(s) 194
BstUI CGCG 4 cut(s) 16, 18, 143, 370
BstV1I GCAGC 2 cut(s) 144, 359
BstXI CCANNNNNNTGG 1 cut(s) 239
BsuRI GGCC 2 cut(s) 338, 428
BtgI CCRYGG 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 364, 369
Cac8I GCNNGC 3 cut(s) 18, 43, 170
CfoI GCGC 3 cut(s) 18, 20, 372
Cfr13I GGNCC 1 cut(s) 34
Cfr42I CCGCGG 1 cut(s) 144
CviAII CATG 2 cut(s) 38, 424
DdeI CTNAG 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 51, 239
EarI CTCTTC 2 cut(s) 51, 239
Ecl136II GAGCTC 2 cut(s) 61, 150
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 2 cut(s) 63, 152
Eco47I GGWCC 1 cut(s) 34
Eco53kI GAGCTC 2 cut(s) 61, 150
Eco57I CTGAAG 1 cut(s) 364
EcoICRI GAGCTC 2 cut(s) 61, 150
EcoT38I GRGCYC 2 cut(s) 63, 152
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 2 cut(s) 41, 427
FaiI YATR 2 cut(s) 39, 425
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 2 cut(s) 219, 263
FatI CATG 2 cut(s) 37, 423
Fnu4HI GCNGC 4 cut(s) 21, 83, 158, 348
FokI GGATG 2 cut(s) 371, 376
FriOI GRGCYC 2 cut(s) 63, 152
Fsp4HI GCNGC 4 cut(s) 21, 83, 158, 348
FspBI CTAG 1 cut(s) 111
GlaI GCGC 3 cut(s) 17, 19, 371
GluI GCNGC 4 cut(s) 21, 83, 158, 348
GsaI CCCAGC 1 cut(s) 206
HaeIII GGCC 2 cut(s) 338, 428
HapII CCGG 1 cut(s) 196
HhaI GCGC 3 cut(s) 18, 20, 372
Hin1II CATG 2 cut(s) 41, 427
Hin6I GCGC 3 cut(s) 16, 18, 370
HinP1I GCGC 3 cut(s) 16, 18, 370
HinfI GANTC 2 cut(s) 191, 222
HpaII CCGG 1 cut(s) 196
HphI GGTGA 2 cut(s) 3, 207
Hpy188III TCNNGA 3 cut(s) 76, 313, 382
Hpy99I CGWCG 2 cut(s) 180, 260
HpyAV CCTTC 2 cut(s) 173, 361
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 140, 178, 344, 403
HpyF3I CTNAG 1 cut(s) 333
Hsp92II CATG 2 cut(s) 41, 427
HspAI GCGC 3 cut(s) 16, 18, 370
KspI CCGCGG 1 cut(s) 144
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 2 cut(s) 165, 384
LpnPI CCDG 7 cut(s) 55, 188, 209, 245, 290, 367, 386
Lsp1109I GCAGC 2 cut(s) 144, 359
LweI GCATC 3 cut(s) 69, 77, 144
MaeI CTAG 1 cut(s) 111
MboII GAAGA 3 cut(s) 68, 256, 370
MhlI GDGCHC 2 cut(s) 63, 152
MluCI AATT 1 cut(s) 454
MnlI CCTC 4 cut(s) 24, 228, 240, 328
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 1 cut(s) 196
MvnI CGCG 4 cut(s) 16, 18, 143, 370
MwoI GCNNNNNNNGC 5 cut(s) 13, 140, 178, 344, 403
NciI CCSGG 1 cut(s) 196
NlaIII CATG 2 cut(s) 41, 427
NlaIV GGNNCC 2 cut(s) 35, 396
PauI GCGCGC 1 cut(s) 16
PceI AGGCCT 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 172
PfeI GAWTC 2 cut(s) 191, 222
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 4 cut(s) 22, 84, 159, 349
Psp124BI GAGCTC 2 cut(s) 63, 152
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 2 cut(s) 35, 396
PspPI GGNCC 1 cut(s) 34
PsyI GACNNNGTC 1 cut(s) 254
PteI GCGCGC 1 cut(s) 16
SacI GAGCTC 2 cut(s) 63, 152
SacII CCGCGG 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 4 cut(s) 21, 83, 158, 348
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 2 cut(s) 63, 152
SetI ASST 7 cut(s) 63, 152, 220, 239, 279, 389, 453
SfaNI GCATC 3 cut(s) 69, 77, 144
Sfr303I CCGCGG 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 144
SinI GGWCC 1 cut(s) 34
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 1 cut(s) 454
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 5 cut(s) 14, 21, 82, 141, 143
SspMI CTAG 1 cut(s) 111
SstI GAGCTC 2 cut(s) 63, 152
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 194
TaqI TCGA 3 cut(s) 29, 152, 220
TasI AATT 1 cut(s) 454
TauI GCSGC 2 cut(s) 23, 85
TfiI GAWTC 2 cut(s) 191, 222
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TseI GCWGC 2 cut(s) 157, 347
Tth111I GACNNNGTC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 34
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.