Rh6AG078500

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0004886
Physical Location & Seq
Forward (+)
11416632 .. 11422954
6323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG078500.1

Sequence Viewer

Length: 537 bp
ATGCGGAGGCGCGACAAAGACAAGGAGAAAACTGGAACTTTCAGCTACTTCCAAGCAAGAACCGCGTCGAGCTCGATGCTGCTCCTCCGCTTGCTACGACGGCGTTTTGGGTTGTTAAAAGCTTCAGTCTTTTGTTACTCCCAGTTAGATTTTTTTGGTGTTGATTTTGATGATGGTTTTGTGCACATTTTCAAGCTGCCTACTCACACCGGCACGCATGTTGATTCACCCGGACACTTCTTCGATCACTACCTCGATGCTGGCTTCGACGTCGATACGCTTGACTTGGAGGTGCTTAATGGTCCAGCATTGTTAGTTGATGTTCCGAGGGACAAGAACATAACTGCTGAAGTGATGAAGTCCTTAAATATTCCAAAGGGAGTAGGTCGTGTGCTCTTCAGAACATTAAATACTGACAGGATAAGTATAATGTCAAGACTCGTGAGCACTAAGGAAACAGCAAACAGCCCCGGGAAAAGTTGCATAGCAATGAAACGGACAGCATCCAAGTTCTTGCAAGGAGTGGAAAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

178

Amino Acids

20.07

Weight (kDa)

9.58

Isoelectric Point (pI)

43.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyclase PF04199 56 - 165 5.7e-11 Putative cyclase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 273
AccII CGCG 2 cut(s) 12, 65
AciI CCGC 3 cut(s) 4, 63, 88
AcuI CTGAAG 3 cut(s) 108, 369, 382
AcyI GRCGYC 1 cut(s) 270
AgsI TTSAA 1 cut(s) 193
AjiI CACGTC 1 cut(s) 534
AluBI AGCT 4 cut(s) 45, 72, 122, 196
AluI AGCT 4 cut(s) 45, 72, 122, 196
Alw21I GWGCWC 4 cut(s) 74, 186, 396, 449
Alw44I GTGCAC 1 cut(s) 182
Ama87I CYCGRG 1 cut(s) 470
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 2 cut(s) 79, 196
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 3 cut(s) 231, 471, 472
AsuHPI GGTGA 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 74
BauI CACGAG 1 cut(s) 440
Bbv12I GWGCWC 4 cut(s) 74, 186, 396, 449
BbvI GCAGC 2 cut(s) 66, 183
BccI CCATC 1 cut(s) 167
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BcnI CCSGG 3 cut(s) 231, 471, 472
BisI GCNGC 2 cut(s) 80, 197
BlsI GCNGC 2 cut(s) 81, 198
Bme1390I CCNGG 3 cut(s) 231, 471, 472
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 470
BmgBI CACGTC 1 cut(s) 534
BmgT120I GGNCC 1 cut(s) 302
BmrFI CCNGG 3 cut(s) 231, 471, 472
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 3 cut(s) 66, 247, 512
BmuI ACTGGG 1 cut(s) 136
BpuMI CCSGG 3 cut(s) 231, 471, 472
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 326, 469, 470
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 2 cut(s) 37, 142
Bse3DI GCAATG 1 cut(s) 495
BseDI CCNNGG 3 cut(s) 326, 469, 470
BseGI GGATG 1 cut(s) 503
BseMI GCAATG 1 cut(s) 495
BseNI ACTGG 2 cut(s) 37, 142
BseRI GAGGAG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 2 cut(s) 66, 183
Bsh1236I CGCG 2 cut(s) 12, 65
BsiHKAI GWGCWC 4 cut(s) 74, 186, 396, 449
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 3 cut(s) 210, 231, 471
BslFI GGGAC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 344
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 4 cut(s) 74, 186, 396, 449
Bsp143I GATC 1 cut(s) 244
BspACI CCGC 3 cut(s) 4, 63, 88
BspFNI CGCG 2 cut(s) 12, 65
BspQI GCTCTTC 1 cut(s) 401
BsrDI GCAATG 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 2 cut(s) 37, 142
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 326, 469, 470
BssMI GATC 1 cut(s) 244
BssNI GRCGYC 1 cut(s) 270
BssSI CACGAG 1 cut(s) 440
Bst2BI CACGAG 1 cut(s) 440
Bst6I CTCTTC 1 cut(s) 401
BstACI GRCGYC 1 cut(s) 270
BstC8I GCNNGC 3 cut(s) 92, 215, 262
BstDEI CTNAG 1 cut(s) 450
BstF5I GGATG 1 cut(s) 503
BstFNI CGCG 2 cut(s) 12, 65
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 62, 100
BstNSI RCATGY 1 cut(s) 221
BstSCI CCNGG 3 cut(s) 229, 469, 470
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 2 cut(s) 12, 65
BstV1I GCAGC 2 cut(s) 66, 183
BtrI CACGTC 1 cut(s) 534
BtsCI GGATG 1 cut(s) 503
Cac8I GCNNGC 3 cut(s) 92, 215, 262
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 1 cut(s) 302
Cfr9I CCCGGG 1 cut(s) 470
CseI GACGC 1 cut(s) 54
CviAII CATG 1 cut(s) 218
CviJI RGCY 6 cut(s) 45, 72, 122, 196, 264, 468
CviKI_1 RGCY 6 cut(s) 45, 72, 122, 196, 264, 468
DdeI CTNAG 1 cut(s) 450
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 302
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 3 cut(s) 108, 369, 382
Eco88I CYCGRG 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 74
FaeI CATG 1 cut(s) 221
FaiI YATR 4 cut(s) 219, 341, 428, 485
FaqI GGGAC 1 cut(s) 344
FatI CATG 1 cut(s) 217
Fnu4HI GCNGC 2 cut(s) 80, 197
FokI GGATG 1 cut(s) 490
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 2 cut(s) 80, 197
GlaI GCGC 1 cut(s) 11
GluI GCNGC 2 cut(s) 80, 197
HapII CCGG 3 cut(s) 210, 231, 471
HgaI GACGC 1 cut(s) 54
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 1 cut(s) 221
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 2 cut(s) 224, 438
HpaII CCGG 3 cut(s) 210, 231, 471
HphI GGTGA 1 cut(s) 219
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 327, 401
Hpy188III TCNNGA 2 cut(s) 435, 442
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 4 cut(s) 70, 102, 272, 275
HpyCH4IV ACGT 2 cut(s) 270, 533
HpyCH4V TGCA 3 cut(s) 184, 483, 517
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 100
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 270, 533
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 1 cut(s) 221
HspAI GCGC 1 cut(s) 10
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 401
LmnI GCTCC 1 cut(s) 87
LpnPI CCDG 8 cut(s) 18, 155, 223, 244, 246, 318, 403, 484
Lsp1109I GCAGC 2 cut(s) 66, 183
LweI GCATC 3 cut(s) 66, 247, 512
MaeII ACGT 2 cut(s) 270, 533
MaeIII GTNAC 1 cut(s) 134
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 2 cut(s) 232, 388
MhlI GDGCHC 4 cut(s) 74, 186, 396, 449
MlyI GAGTC 1 cut(s) 432
MnlI CCTC 4 cut(s) 95, 263, 283, 321
MseI TTAA 4 cut(s) 116, 297, 365, 407
MslI CAYNNNNRTG 1 cut(s) 488
MspI CCGG 3 cut(s) 210, 231, 471
MspR9I CCNGG 3 cut(s) 231, 471, 472
MvnI CGCG 2 cut(s) 12, 65
MwoI GCNNNNNNNGC 2 cut(s) 62, 100
NciI CCSGG 3 cut(s) 231, 471, 472
NdeII GATC 1 cut(s) 244
NlaIII CATG 1 cut(s) 221
NspI RCATGY 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 401
PfeI GAWTC 1 cut(s) 224
PkrI GCNGC 2 cut(s) 81, 198
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 74
PspPI GGNCC 1 cut(s) 302
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 4 cut(s) 116, 297, 365, 407
SatI GCNGC 2 cut(s) 80, 197
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 1 cut(s) 432
ScrFI CCNGG 3 cut(s) 231, 471, 472
SduI GDGCHC 4 cut(s) 74, 186, 396, 449
SetI ASST 9 cut(s) 47, 74, 124, 198, 255, 273, 294, 388, 536
SfaNI GCATC 3 cut(s) 66, 247, 512
SinI GGWCC 1 cut(s) 302
SmaI CCCGGG 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 488
SsiI CCGC 3 cut(s) 4, 63, 88
SspI AATATT 1 cut(s) 370
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 3 cut(s) 229, 469, 470
TaiI ACGT 2 cut(s) 273, 536
TaqI TCGA 6 cut(s) 68, 74, 243, 255, 267, 273
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 4 cut(s) 116, 297, 365, 407
Tru9I TTAA 4 cut(s) 116, 297, 365, 407
TseI GCWGC 2 cut(s) 79, 196
TspDTI ATGAA 2 cut(s) 371, 506
TspGWI ACGGA 1 cut(s) 511
TspMI CCCGGG 1 cut(s) 470
VneI GTGCAC 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 302
XceI RCATGY 1 cut(s) 221
XmaI CCCGGG 1 cut(s) 470
ZraI GACGTC 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.