Rroxscaffold_5G00350040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004886
Physical Location & Seq
Forward (+)
23578734 .. 23583532
4799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350040.1

Sequence Viewer

Length: 357 bp
ATGAGTGAGCGTACAAAGATTCTGGAGGATATAATTTTAAAGATGAGCCAAAATGGGCATGGTGGTGGTGGTGGTGGAAATATGCATGCTGATCCTGTTGGTACTCCACAATGTCTTTGCTGTTGGCATTGTCGGCCACGAAAGGGACTTGTTTTAAAAAAAAAAAGGGGGAGGAAAGCTTTCATCCATGGCCGAAATGCCGGTGGAGAGATCCTTCGAGGCTTGGGAGGAATTGCAGCGTCACGGCCAGGACTTAGCAGATCATCCGGTGCAAGGTTCCACCGGTCTGATTCGCACCCACCTCTTTACGCCGTCATTTCCGTGGCCGAGCTCTCAATTCGAAACCGTTCGACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

12.67

Weight (kDa)

10.9

Isoelectric Point (pI)

70.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 86, 205
AcoI YGGCCR 4 cut(s) 134, 190, 245, 324
AfaI GTAC 2 cut(s) 13, 103
AfiI CCNNNNNNNGG 2 cut(s) 143, 273
AgeI ACCGGT 1 cut(s) 282
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 2 cut(s) 179, 331
AluI AGCT 2 cut(s) 179, 331
Alw21I GWGCWC 1 cut(s) 333
AlwI GGATC 2 cut(s) 86, 205
AoxI GGCC 4 cut(s) 134, 190, 245, 324
ApeKI GCWGC 1 cut(s) 236
AsiGI ACCGGT 1 cut(s) 282
Asp700I GAANNNNTTC 2 cut(s) 179, 346
AsuII TTCGAA 1 cut(s) 340
BanII GRGCYC 1 cut(s) 333
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 248
BceAI ACGGC 2 cut(s) 260, 296
BciT130I CCWGG 1 cut(s) 249
BisI GCNGC 1 cut(s) 237
BlsI GCNGC 1 cut(s) 238
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 278
BmrFI CCNGG 1 cut(s) 249
BpmI CTGGAG 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 340
BsaJI CCNNGG 2 cut(s) 187, 321
BsaWI WCCGGW 2 cut(s) 266, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 273
Bse118I RCCGGY 2 cut(s) 200, 282
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 187, 321
BseGI GGATG 2 cut(s) 183, 263
BseLI CCNNNNNNNGG 2 cut(s) 143, 273
BseXI GCAGC 1 cut(s) 248
BshFI GGCC 4 cut(s) 136, 192, 247, 326
BshTI ACCGGT 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 333
BsiSI CCGG 3 cut(s) 201, 267, 283
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 2 cut(s) 143, 273
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 4 cut(s) 136, 192, 247, 326
Bsp119I TTCGAA 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 3 cut(s) 91, 210, 260
Bsp19I CCATGG 1 cut(s) 187
BspANI GGCC 4 cut(s) 136, 192, 247, 326
BspLI GGNNCC 1 cut(s) 278
BspPI GGATC 2 cut(s) 86, 205
BspT104I TTCGAA 1 cut(s) 340
BsrFI RCCGGY 2 cut(s) 200, 282
BssAI RCCGGY 2 cut(s) 200, 282
BssECI CCNNGG 2 cut(s) 187, 321
BssMI GATC 3 cut(s) 91, 210, 260
BssT1I CCWWGG 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 347
BstBI TTCGAA 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 2 cut(s) 187, 321
BstF5I GGATG 2 cut(s) 183, 263
BstKTI GATC 3 cut(s) 94, 213, 263
BstMBI GATC 3 cut(s) 91, 210, 260
BstMWI GCNNNNNNNGC 1 cut(s) 133
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 4 cut(s) 136, 192, 247, 326
BtgI CCRYGG 2 cut(s) 187, 321
BtsCI GGATG 2 cut(s) 183, 263
Cac8I GCNNGC 1 cut(s) 87
Cfr10I RCCGGY 2 cut(s) 200, 282
CseI GACGC 1 cut(s) 228
Csp6I GTAC 2 cut(s) 12, 102
CspAI ACCGGT 1 cut(s) 282
CviAII CATG 3 cut(s) 59, 86, 188
CviJI RGCY 8 cut(s) 48, 136, 179, 192, 222, 247, 326, 331
CviKI_1 RGCY 8 cut(s) 48, 136, 179, 192, 222, 247, 326, 331
CviQI GTAC 2 cut(s) 12, 102
DdeI CTNAG 1 cut(s) 254
DpnI GATC 3 cut(s) 93, 212, 262
DpnII GATC 3 cut(s) 91, 210, 260
DraI TTTAAA 2 cut(s) 39, 156
EaeI YGGCCR 4 cut(s) 134, 190, 245, 324
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 187
Eco24I GRGCYC 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 3 cut(s) 62, 89, 191
FaiI YATR 5 cut(s) 32, 60, 83, 87, 189
FaqI GGGAC 1 cut(s) 159
FatI CATG 3 cut(s) 58, 85, 187
Fnu4HI GCNGC 1 cut(s) 237
FokI GGATG 2 cut(s) 170, 250
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 237
GluI GCNGC 1 cut(s) 237
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 4 cut(s) 136, 192, 247, 326
HapII CCGG 3 cut(s) 201, 267, 283
HgaI GACGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 62, 89, 191
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 2 cut(s) 19, 290
HpaII CCGG 3 cut(s) 201, 267, 283
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 1 cut(s) 23
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4V TGCA 3 cut(s) 85, 236, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 133
HpyF3I CTNAG 1 cut(s) 254
Hsp92II CATG 3 cut(s) 62, 89, 191
Kzo9I GATC 3 cut(s) 91, 210, 260
LpnPI CCDG 7 cut(s) 8, 108, 214, 234, 261, 280, 296
Lsp1109I GCAGC 1 cut(s) 248
MaeIII GTNAC 1 cut(s) 240
MalI GATC 3 cut(s) 93, 212, 262
MboI GATC 3 cut(s) 91, 210, 260
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 3 cut(s) 33, 231, 336
MnlI CCTC 5 cut(s) 19, 165, 212, 221, 312
Mph1103I ATGCAT 1 cut(s) 87
MroXI GAANNNNTTC 2 cut(s) 179, 346
MseI TTAA 2 cut(s) 38, 155
MslI CAYNNNNRTG 2 cut(s) 63, 320
MspI CCGG 3 cut(s) 201, 267, 283
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 1 cut(s) 133
NcoI CCATGG 1 cut(s) 187
NdeII GATC 3 cut(s) 91, 210, 260
NlaIII CATG 3 cut(s) 62, 89, 191
NlaIV GGNNCC 1 cut(s) 278
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 87
NspI RCATGY 1 cut(s) 89
NspV TTCGAA 1 cut(s) 340
PaeI GCATGC 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 179, 346
PfeI GAWTC 2 cut(s) 19, 290
PinAI ACCGGT 1 cut(s) 282
PkrI GCNGC 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 278
PsuI RGATCY 1 cut(s) 210
RsaI GTAC 2 cut(s) 13, 103
RsaNI GTAC 2 cut(s) 12, 102
RseI CAYNNNNRTG 2 cut(s) 63, 320
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 38, 155
SatI GCNGC 1 cut(s) 237
Sau3AI GATC 3 cut(s) 91, 210, 260
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 333
SetI ASST 5 cut(s) 181, 278, 304, 333, 356
SfuI TTCGAA 1 cut(s) 340
SmiMI CAYNNNNRTG 2 cut(s) 63, 320
SphI GCATGC 1 cut(s) 89
Sse9I AATT 3 cut(s) 33, 231, 336
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 247
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 1 cut(s) 347
TaqI TCGA 3 cut(s) 217, 340, 350
TasI AATT 3 cut(s) 33, 231, 336
TfiI GAWTC 2 cut(s) 19, 290
Tru1I TTAA 2 cut(s) 38, 155
Tru9I TTAA 2 cut(s) 38, 155
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 1 cut(s) 172
TspGWI ACGGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 89
XcmI CCANNNNNNNNNTGG 1 cut(s) 56
XmnI GAANNNNTTC 2 cut(s) 179, 346
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.