Rroxscaffold_4G00324080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0004886
Physical Location & Seq
Reverse (-)
55386193 .. 55386717
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324080.1

Sequence Viewer

Length: 525 bp
ATGCCGGTGGAGAGATTCTTCAAGGCTTGGGAGGAGTTGCAGCATCACGGCCGGGGTCCTAGCAGATTGTCCGGTGCAAGGTTTCACCGGTTTGATTCGGACCCACCTCTATACGCCGTCGTTTCCATGGCCGAGCTCTCACAATTCGGGAATCGTTCGACCTTGAGTTCCCGAGCCGAACATATGGAAGAGGGACTTGGTCGGGTTGGTGGCGGAGAACCCGGAATCAATGGGTTTCTGCGATTCGGACATAGGGTTTTGAGGTCGGCTTCGGGGAAGAACCGGAGCCTCGAGCACGGAGCCTCAAGTCCGAAGCCCGAAGAGAGCAAAGCCGAAGGTCGTAAAATGAGAGAGAGTGAGGATGAGCCTCGTTTTGGAGGTTGCTTTCTTCTTAAGTCGCTGCCTTGCCCGATGTGGATGGGCAAACTTCGACGCCGGAGCAGAAGGAGGTGTGCCAAGCAATTGTCAATAGGCTGCCGGAGATTAATCGGCTCTCGACAACACAATGCAGCAGCTCTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.53

Weight (kDa)

10.78

Isoelectric Point (pI)

75.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AcoI YGGCCR 2 cut(s) 49, 129
AcyI GRCGYC 1 cut(s) 433
AfiI CCNNNNNNNGG 2 cut(s) 78, 374
AflII CTTAAG 1 cut(s) 392
AgeI ACCGGT 1 cut(s) 87
AgsI TTSAA 1 cut(s) 22
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 2 cut(s) 136, 515
AluI AGCT 2 cut(s) 136, 515
Alw21I GWGCWC 2 cut(s) 138, 297
Ama87I CYCGRG 2 cut(s) 171, 290
AoxI GGCC 2 cut(s) 49, 129
ApeKI GCWGC 5 cut(s) 40, 400, 474, 509, 512
AseI ATTAAT 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 87
AspS9I GGNCC 2 cut(s) 56, 100
AsuC2I CCSGG 2 cut(s) 53, 222
AsuHPI GGTGA 1 cut(s) 77
AvaI CYCGRG 2 cut(s) 171, 290
AvaII GGWCC 2 cut(s) 56, 100
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 138, 297
BbvI GCAGC 4 cut(s) 52, 387, 461, 521
BccI CCATC 1 cut(s) 412
BceAI ACGGC 2 cut(s) 64, 101
BcnI CCSGG 2 cut(s) 53, 222
BfaI CTAG 1 cut(s) 60
BfrI CTTAAG 1 cut(s) 392
BisI GCNGC 5 cut(s) 41, 401, 475, 510, 513
BlsI GCNGC 5 cut(s) 42, 402, 476, 511, 514
Bme1390I CCNGG 2 cut(s) 53, 222
Bme18I GGWCC 2 cut(s) 56, 100
BmeT110I CYCGRG 2 cut(s) 171, 290
BmgT120I GGNCC 2 cut(s) 56, 100
BmiI GGNNCC 4 cut(s) 57, 102, 287, 301
BmrFI CCNGG 2 cut(s) 53, 222
BmsI GCATC 1 cut(s) 52
BpuEI CTTGAG 2 cut(s) 184, 289
BpuMI CCSGG 2 cut(s) 53, 222
BsaHI GRCGYC 1 cut(s) 433
BsaJI CCNNGG 2 cut(s) 52, 126
BsaWI WCCGGW 3 cut(s) 71, 87, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 374
Bse118I RCCGGY 2 cut(s) 4, 87
BseDI CCNNGG 2 cut(s) 52, 126
BseGI GGATG 2 cut(s) 367, 423
BseLI CCNNNNNNNGG 2 cut(s) 78, 374
BseRI GAGGAG 1 cut(s) 47
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 4 cut(s) 52, 387, 461, 521
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 2 cut(s) 51, 131
BshTI ACCGGT 1 cut(s) 87
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 138, 297
BsiHKCI CYCGRG 2 cut(s) 171, 290
BsiSI CCGG 8 cut(s) 5, 52, 72, 88, 222, 283, 436, 478
BslFI GGGAC 1 cut(s) 207
BslI CCNNNNNNNGG 2 cut(s) 78, 374
BsmFI GGGAC 1 cut(s) 207
BsnI GGCC 2 cut(s) 51, 131
BsoBI CYCGRG 2 cut(s) 171, 290
Bsp1286I GDGCHC 2 cut(s) 138, 297
Bsp19I CCATGG 1 cut(s) 126
BspACI CCGC 1 cut(s) 213
BspANI GGCC 2 cut(s) 51, 131
BspLI GGNNCC 4 cut(s) 57, 102, 287, 301
BspTI CTTAAG 1 cut(s) 392
BsrFI RCCGGY 2 cut(s) 4, 87
BssAI RCCGGY 2 cut(s) 4, 87
BssECI CCNNGG 2 cut(s) 52, 126
BssNI GRCGYC 1 cut(s) 433
BssT1I CCWWGG 1 cut(s) 126
Bst4CI ACNGT 1 cut(s) 522
Bst6I CTCTTC 2 cut(s) 183, 315
BstACI GRCGYC 1 cut(s) 433
BstAFI CTTAAG 1 cut(s) 392
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 367, 423
BstMCI CGRYCG 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 51, 220
BstV1I GCAGC 4 cut(s) 52, 387, 461, 521
BstZI CGGCCG 1 cut(s) 49
BsuRI GGCC 2 cut(s) 51, 131
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 2 cut(s) 367, 423
Cfr10I RCCGGY 2 cut(s) 4, 87
Cfr13I GGNCC 2 cut(s) 56, 100
CseI GACGC 1 cut(s) 441
CspAI ACCGGT 1 cut(s) 87
CviAII CATG 1 cut(s) 127
EaeI YGGCCR 2 cut(s) 49, 129
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 2 cut(s) 183, 315
EarI CTCTTC 2 cut(s) 183, 315
EciI GGCGGA 1 cut(s) 228
Ecl136II GAGCTC 1 cut(s) 136
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 56, 100
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 171, 290
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 126
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 1 cut(s) 130
FaiI YATR 5 cut(s) 112, 128, 183, 185, 252
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 207
FatI CATG 1 cut(s) 126
FauNDI CATATG 1 cut(s) 183
Fnu4HI GCNGC 5 cut(s) 41, 401, 475, 510, 513
FokI GGATG 2 cut(s) 374, 430
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 5 cut(s) 41, 401, 475, 510, 513
FspBI CTAG 1 cut(s) 60
GluI GCNGC 5 cut(s) 41, 401, 475, 510, 513
HaeIII GGCC 2 cut(s) 51, 131
HapII CCGG 8 cut(s) 5, 52, 72, 88, 222, 283, 436, 478
HgaI GACGC 1 cut(s) 441
Hin1I GRCGYC 1 cut(s) 433
Hin1II CATG 1 cut(s) 130
HinfI GANTC 5 cut(s) 15, 95, 151, 225, 243
HpaII CCGG 8 cut(s) 5, 52, 72, 88, 222, 283, 436, 478
HphI GGTGA 1 cut(s) 77
Hpy188I TCNGA 3 cut(s) 100, 248, 312
Hpy188III TCNNGA 3 cut(s) 148, 171, 495
Hpy99I CGWCG 2 cut(s) 122, 435
HpyAV CCTTC 2 cut(s) 329, 438
HpyCH4III ACNGT 1 cut(s) 522
HpyCH4V TGCA 3 cut(s) 40, 77, 509
Hsp92I GRCGYC 1 cut(s) 433
Hsp92II CATG 1 cut(s) 130
LmnI GCTCC 3 cut(s) 285, 299, 438
LpnPI CCDG 8 cut(s) 18, 65, 85, 101, 235, 296, 449, 491
Lsp1109I GCAGC 4 cut(s) 52, 387, 461, 521
LweI GCATC 1 cut(s) 52
MaeI CTAG 1 cut(s) 60
MboII GAAGA 5 cut(s) 10, 200, 289, 332, 380
MfeI CAATTG 1 cut(s) 461
MhlI GDGCHC 2 cut(s) 138, 297
MluCI AATT 2 cut(s) 143, 461
MseI TTAA 2 cut(s) 393, 485
MspCI CTTAAG 1 cut(s) 392
MspI CCGG 8 cut(s) 5, 52, 72, 88, 222, 283, 436, 478
MspR9I CCNGG 2 cut(s) 53, 222
MunI CAATTG 1 cut(s) 461
NciI CCSGG 2 cut(s) 53, 222
NcoI CCATGG 1 cut(s) 126
NdeI CATATG 1 cut(s) 183
NlaIII CATG 1 cut(s) 130
NlaIV GGNNCC 4 cut(s) 57, 102, 287, 301
NmeAIII GCCGAG 1 cut(s) 157
PaeR7I CTCGAG 1 cut(s) 290
PfeI GAWTC 5 cut(s) 15, 95, 151, 225, 243
PflFI GACNNNGTC 1 cut(s) 198
PinAI ACCGGT 1 cut(s) 87
PkrI GCNGC 5 cut(s) 42, 402, 476, 511, 514
PpuMI RGGWCCY 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 57, 102, 287, 301
PspPI GGNCC 2 cut(s) 56, 100
PspPPI RGGWCCY 1 cut(s) 56
PspXI VCTCGAGB 1 cut(s) 290
PsyI GACNNNGTC 1 cut(s) 198
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 393, 485
SatI GCNGC 5 cut(s) 41, 401, 475, 510, 513
Sau96I GGNCC 2 cut(s) 56, 100
ScrFI CCNGG 2 cut(s) 53, 222
SduI GDGCHC 2 cut(s) 138, 297
SetI ASST 9 cut(s) 83, 109, 138, 164, 266, 340, 382, 452, 517
SfaNI GCATC 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 290
SinI GGWCC 2 cut(s) 56, 100
SlaI CTCGAG 1 cut(s) 290
SmlI CTYRAG 4 cut(s) 163, 290, 304, 392
SmoI CTYRAG 4 cut(s) 163, 290, 304, 392
Sse9I AATT 2 cut(s) 143, 461
SsiI CCGC 1 cut(s) 213
SspMI CTAG 1 cut(s) 60
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 51, 220
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 522
TaqI TCGA 4 cut(s) 158, 291, 430, 496
TasI AATT 2 cut(s) 143, 461
TfiI GAWTC 5 cut(s) 15, 95, 151, 225, 243
Tru1I TTAA 2 cut(s) 393, 485
Tru9I TTAA 2 cut(s) 393, 485
TseI GCWGC 5 cut(s) 40, 400, 474, 509, 512
TspGWI ACGGA 1 cut(s) 312
Tth111I GACNNNGTC 1 cut(s) 198
Vha464I CTTAAG 1 cut(s) 392
VpaK11BI GGWCC 2 cut(s) 56, 100
VspI ATTAAT 1 cut(s) 485
XhoI CTCGAG 1 cut(s) 290
XspI CTAG 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.