Rh5AG494000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004886
Physical Location & Seq
Reverse (-)
84058745 .. 84059074
330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG494000.1

Sequence Viewer

Length: 330 bp
ATGAAATCCCGGCCGTCCGATTCGGCCTCCAACGACGCGTCCGGGTGGTGCAGCTTCGCCAAGCTCCTGTACGCCGACTTGATCTCCGTCTGCGACGCGTTGTTGTTCACCTGCAGAACCTCGTACAGGCTCGCCGGAGACGCAGTCGCCGGCCGCCGCTCCGTCTCGGCGAACGCCCTCACGGAGACCTTCAGCTGCCGGCGAGGCAGGGGCATGTCGGTAAAAGCGGCGGAGGAGAGCTTGTGGTTCGCCGCGGCGGAGAAGGAGAGGCCGGAGAGGGCTAGGGTTGCAGACATTGGCGGTCCGTGGAGGGCAATATGGGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

109

Amino Acids

11.92

Weight (kDa)

8.83

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 119
AccBSI CCGCTC 1 cut(s) 159
AccII CGCG 3 cut(s) 38, 98, 254
AciI CCGC 8 cut(s) 154, 157, 227, 230, 252, 254, 257, 300
AcoI YGGCCR 2 cut(s) 11, 151
AcsI RAATTY 1 cut(s) 324
AcuI CTGAAG 1 cut(s) 175
AfaI GTAC 2 cut(s) 71, 125
AfiI CCNNNNNNNGG 1 cut(s) 126
AflIII ACRYGT 2 cut(s) 36, 96
AluBI AGCT 4 cut(s) 54, 64, 195, 240
AluI AGCT 4 cut(s) 54, 64, 195, 240
Alw26I GTCTC 3 cut(s) 132, 169, 179
AoxI GGCC 4 cut(s) 11, 24, 151, 269
ApeKI GCWGC 2 cut(s) 51, 195
ApoI RAATTY 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 302
AsuC2I CCSGG 2 cut(s) 10, 43
AsuHPI GGTGA 1 cut(s) 100
AvaII GGWCC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 63, 182
BcnI CCSGG 2 cut(s) 10, 43
BcoDI GTCTC 3 cut(s) 132, 169, 179
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 112
BfuAI ACCTGC 1 cut(s) 119
BglI GCCNNNNNGGC 1 cut(s) 204
BisI GCNGC 7 cut(s) 52, 154, 157, 196, 228, 252, 255
BlsI GCNGC 7 cut(s) 53, 155, 158, 197, 229, 253, 256
Bme1390I CCNGG 2 cut(s) 10, 43
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmrFI CCNGG 2 cut(s) 10, 43
BpuMI CCSGG 2 cut(s) 10, 43
BsaI GGTCTC 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 252, 305
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 129, 159, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 126
Bse118I RCCGGY 2 cut(s) 149, 198
BseDI CCNNGG 2 cut(s) 252, 305
BseLI CCNNNNNNNGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 248
BseX3I CGGCCG 2 cut(s) 11, 151
BseXI GCAGC 2 cut(s) 63, 182
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 3 cut(s) 38, 98, 254
Bsh1285I CGRYCG 2 cut(s) 14, 154
BshFI GGCC 4 cut(s) 13, 26, 153, 271
BsiEI CGRYCG 2 cut(s) 14, 154
BsiSI CCGG 6 cut(s) 10, 42, 135, 150, 199, 272
BslI CCNNNNNNNGG 1 cut(s) 126
BsmAI GTCTC 3 cut(s) 132, 169, 179
BsmBI CGTCTC 2 cut(s) 132, 169
BsnI GGCC 4 cut(s) 13, 26, 153, 271
Bso31I GGTCTC 1 cut(s) 179
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 8 cut(s) 154, 157, 227, 230, 252, 254, 257, 300
BspANI GGCC 4 cut(s) 13, 26, 153, 271
BspFNI CGCG 3 cut(s) 38, 98, 254
BspMAI CTGCAG 1 cut(s) 116
BspMI ACCTGC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 179
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 149, 198
BssAI RCCGGY 2 cut(s) 149, 198
BssECI CCNNGG 2 cut(s) 252, 305
BssMI GATC 1 cut(s) 81
BstC8I GCNNGC 3 cut(s) 132, 151, 200
BstDSI CCRYGG 2 cut(s) 252, 305
BstENI CCTNNNNNAGG 1 cut(s) 124
BstFNI CGCG 3 cut(s) 38, 98, 254
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 3 cut(s) 132, 169, 179
BstMBI GATC 1 cut(s) 81
BstMCI CGRYCG 2 cut(s) 14, 154
BstMWI GCNNNNNNNGC 3 cut(s) 140, 204, 287
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 8, 41
BstSFI CTRYAG 1 cut(s) 112
BstUI CGCG 3 cut(s) 38, 98, 254
BstV1I GCAGC 2 cut(s) 63, 182
BstZI CGGCCG 2 cut(s) 11, 151
BsuRI GGCC 4 cut(s) 13, 26, 153, 271
BtgI CCRYGG 2 cut(s) 252, 305
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 132, 151, 200
Cfr10I RCCGGY 2 cut(s) 149, 198
Cfr13I GGNCC 1 cut(s) 302
Cfr42I CCGCGG 1 cut(s) 255
CpoI CGGWCCG 1 cut(s) 302
CseI GACGC 4 cut(s) 27, 44, 104, 149
Csp6I GTAC 2 cut(s) 70, 124
CspI CGGWCCG 1 cut(s) 302
CviAII CATG 1 cut(s) 214
CviQI GTAC 2 cut(s) 70, 124
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
EaeI YGGCCR 2 cut(s) 11, 151
EagI CGGCCG 2 cut(s) 11, 151
EciI GGCGGA 2 cut(s) 245, 272
EclXI CGGCCG 2 cut(s) 11, 151
Eco31I GGTCTC 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 302
Eco52I CGGCCG 2 cut(s) 11, 151
Eco57I CTGAAG 1 cut(s) 175
EcoNI CCTNNNNNAGG 1 cut(s) 124
Esp3I CGTCTC 2 cut(s) 132, 169
FaeI CATG 1 cut(s) 217
FaiI YATR 2 cut(s) 215, 319
FatI CATG 1 cut(s) 213
Fnu4HI GCNGC 7 cut(s) 52, 154, 157, 196, 228, 252, 255
Fsp4HI GCNGC 7 cut(s) 52, 154, 157, 196, 228, 252, 255
FspBI CTAG 1 cut(s) 282
GluI GCNGC 7 cut(s) 52, 154, 157, 196, 228, 252, 255
HaeIII GGCC 4 cut(s) 13, 26, 153, 271
HapII CCGG 6 cut(s) 10, 42, 135, 150, 199, 272
HgaI GACGC 4 cut(s) 27, 44, 104, 149
Hin1II CATG 1 cut(s) 217
HinfI GANTC 1 cut(s) 20
HpaII CCGG 6 cut(s) 10, 42, 135, 150, 199, 272
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 1 cut(s) 19
Hpy8I GTNNAC 1 cut(s) 108
Hpy99I CGWCG 2 cut(s) 38, 98
HpyAV CCTTC 2 cut(s) 199, 256
HpyCH4V TGCA 3 cut(s) 51, 114, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 204, 287
Hsp92II CATG 1 cut(s) 217
KroI GCCGGC 2 cut(s) 149, 198
KroNI GCCGGC 2 cut(s) 151, 200
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 2 cut(s) 69, 164
Lsp1109I GCAGC 2 cut(s) 63, 182
MaeI CTAG 1 cut(s) 282
MalI GATC 1 cut(s) 83
MbiI CCGCTC 1 cut(s) 159
MboI GATC 1 cut(s) 81
MluCI AATT 1 cut(s) 324
MluI ACGCGT 2 cut(s) 36, 96
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 8 cut(s) 37, 130, 188, 197, 226, 261, 270, 303
MroNI GCCGGC 2 cut(s) 149, 198
MseI TTAA 1 cut(s) 328
MspA1I CMGCKG 2 cut(s) 195, 254
MspI CCGG 6 cut(s) 10, 42, 135, 150, 199, 272
MspR9I CCNGG 2 cut(s) 10, 43
MvnI CGCG 3 cut(s) 38, 98, 254
MwoI GCNNNNNNNGC 3 cut(s) 140, 204, 287
NaeI GCCGGC 2 cut(s) 151, 200
NciI CCSGG 2 cut(s) 10, 43
NdeII GATC 1 cut(s) 81
NgoMIV GCCGGC 2 cut(s) 149, 198
NlaIII CATG 1 cut(s) 217
NmeAIII GCCGAG 1 cut(s) 146
NspI RCATGY 1 cut(s) 217
PaqCI CACCTGC 1 cut(s) 119
PdiI GCCGGC 2 cut(s) 151, 200
PfeI GAWTC 1 cut(s) 20
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 7 cut(s) 53, 155, 158, 197, 229, 253, 256
PspPI GGNCC 1 cut(s) 302
PstI CTGCAG 1 cut(s) 116
PsyI GACNNNGTC 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 195
RsaI GTAC 2 cut(s) 71, 125
RsaNI GTAC 2 cut(s) 70, 124
Rsr2I CGGWCCG 1 cut(s) 302
RsrII CGGWCCG 1 cut(s) 302
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 1 cut(s) 328
SatI GCNGC 7 cut(s) 52, 154, 157, 196, 228, 252, 255
Sau3AI GATC 1 cut(s) 81
Sau96I GGNCC 1 cut(s) 302
ScrFI CCNGG 2 cut(s) 10, 43
SetI ASST 7 cut(s) 56, 66, 113, 122, 191, 197, 242
SfcI CTRYAG 1 cut(s) 112
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SinI GGWCC 1 cut(s) 302
Sse9I AATT 1 cut(s) 324
SsiI CCGC 8 cut(s) 154, 157, 227, 230, 252, 254, 257, 300
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 8, 41
TasI AATT 1 cut(s) 324
TauI GCSGC 5 cut(s) 156, 159, 230, 254, 257
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 328
Tru9I TTAA 1 cut(s) 328
TseI GCWGC 2 cut(s) 51, 195
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 4 cut(s) 76, 151, 197, 294
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 302
XagI CCTNNNNNAGG 1 cut(s) 124
XapI RAATTY 1 cut(s) 324
XceI RCATGY 1 cut(s) 217
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.