Rh4BG132200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0004886
Physical Location & Seq
Reverse (-)
22245433 .. 22249161
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG132200.1

Sequence Viewer

Length: 660 bp
ATGGTTGGATCCCCCTCTTGGGTTGGTTCCTCTCTATCTCAACTTCTCTGGTCGCGACAAGGTTTCTGTGTCTCTTCCGAATCTTGGCGGCGGTTGGATCTTAGTTTGCACCATGGCATGCAGTGTCCCGAACCCGGTATTGAAGGTTGTGATGGAGTGGATTTTGGGCCACCGGAGTTTGATTGGCTCAAATCGGAGACTACCATGGCGAACTGTGGTGCTGTGCTACTAATACGCGATGATGCAATCGGGATTATTACTAGGCTGATCATGGCGGTGAAGTTTGCTTTGGCGCCGACGTGGCTGAGAACGGAATCTTCTAAGACTGATGACGGCGCAACCGTGATGCTAACTATAATCGGAAGAAAGAGAGAGGGAGTGGATGATGGCGTGGTGGACGAAGGAGAGATTGTGGGAGATGGAGTGGGAGGAGCGCTTCATGAGGACGATGGAGTCCGTGAAGTCGATGAGGTCCGTCACCAACCCAAACCCGAAGTAGCCATCGCCGCCGCGAGTTTGAGGGTTGAGTCCATTCACGGTGGTGGGGAAGAGCAGAAGCAAAGCGGTGAGGATCAAGGCGGTTCTGGTGTGAAATTAAGGAGAGTCCTCTTCTCCTACCTCTGTGTTGACTACTTGCTTGGATTGATTAGACTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

219

Amino Acids

23.68

Weight (kDa)

4.84

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 452
AccB1I GGYRCC 1 cut(s) 292
AccII CGCG 3 cut(s) 55, 237, 512
AciI CCGC 7 cut(s) 88, 91, 275, 507, 510, 564, 579
AclWI GGATC 4 cut(s) 3, 16, 105, 579
AcyI GRCGYC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 435
AfiI CCNNNNNNNGG 4 cut(s) 18, 19, 84, 134
AgsI TTSAA 1 cut(s) 143
AjiI CACGTC 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 540
Alw26I GTCTC 2 cut(s) 76, 191
AlwI GGATC 4 cut(s) 3, 16, 105, 579
Aor51HI AGCGCT 1 cut(s) 435
AoxI GGCC 1 cut(s) 167
AspLEI GCGC 3 cut(s) 295, 338, 436
AspS9I GGNCC 2 cut(s) 167, 472
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 3 cut(s) 289, 470, 578
AvaII GGWCC 1 cut(s) 472
BamHI GGATCC 1 cut(s) 8
BanI GGYRCC 1 cut(s) 292
BccI CCATC 5 cut(s) 146, 380, 413, 443, 509
BceAI ACGGC 1 cut(s) 349
BclI TGATCA 1 cut(s) 267
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 76, 191
BfaI CTAG 1 cut(s) 261
BfoI RGCGCY 2 cut(s) 296, 437
BglI GCCNNNNNGGC 1 cut(s) 301
BisI GCNGC 3 cut(s) 89, 507, 510
BlsI GCNGC 3 cut(s) 90, 508, 511
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 472
BmgBI CACGTC 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 167, 472
BmiI GGNNCC 3 cut(s) 10, 28, 294
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 232, 336
BpuMI CCSGG 1 cut(s) 135
BsaHI GRCGYC 1 cut(s) 293
BsaJI CCNNGG 2 cut(s) 112, 204
BsaWI WCCGGW 1 cut(s) 172
BsaXI ACNNNNNCTCC 4 cut(s) 396, 408, 426, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 18, 19, 84, 134
BseDI CCNNGG 2 cut(s) 112, 204
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 4 cut(s) 18, 19, 84, 134
BseMII CTCAG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 444
Bsh1236I CGCG 3 cut(s) 55, 237, 512
BshFI GGCC 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 292
BsiSI CCGG 2 cut(s) 135, 173
BslFI GGGAC 1 cut(s) 111
BslI CCNNNNNNNGG 4 cut(s) 18, 19, 84, 134
BsmAI GTCTC 2 cut(s) 76, 191
BsmFI GGGAC 1 cut(s) 111
BsnI GGCC 1 cut(s) 169
Bsp143I GATC 4 cut(s) 8, 97, 267, 571
Bsp19I CCATGG 2 cut(s) 112, 204
Bsp68I TCGCGA 1 cut(s) 55
BspACI CCGC 7 cut(s) 88, 91, 275, 507, 510, 564, 579
BspANI GGCC 1 cut(s) 169
BspCNI CTCAG 1 cut(s) 297
BspFNI CGCG 3 cut(s) 55, 237, 512
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 10, 28, 294
BspPI GGATC 4 cut(s) 3, 16, 105, 579
BspQI GCTCTTC 1 cut(s) 543
BspT107I GGYRCC 1 cut(s) 292
BssECI CCNNGG 2 cut(s) 112, 204
BssMI GATC 4 cut(s) 8, 97, 267, 571
BssNI GRCGYC 1 cut(s) 293
BssT1I CCWWGG 2 cut(s) 112, 204
Bst4CI ACNGT 3 cut(s) 215, 343, 539
Bst6I CTCTTC 3 cut(s) 79, 543, 614
BstACI GRCGYC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 101, 305, 321
BstDSI CCRYGG 2 cut(s) 112, 204
BstF5I GGATG 1 cut(s) 388
BstFNI CGCG 3 cut(s) 55, 237, 512
BstH2I RGCGCY 2 cut(s) 296, 437
BstHHI GCGC 3 cut(s) 295, 338, 436
BstKTI GATC 4 cut(s) 11, 100, 270, 574
BstMAI GTCTC 2 cut(s) 76, 191
BstMBI GATC 4 cut(s) 8, 97, 267, 571
BstMWI GCNNNNNNNGC 2 cut(s) 301, 506
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 3 cut(s) 55, 237, 512
BstX2I RGATCY 2 cut(s) 8, 97
BstYI RGATCY 2 cut(s) 8, 97
BsuRI GGCC 1 cut(s) 169
BtgI CCRYGG 2 cut(s) 112, 204
BtgZI GCGATG 2 cut(s) 252, 487
BtrI CACGTC 1 cut(s) 300
BtsCI GGATG 1 cut(s) 388
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
BtuMI TCGCGA 1 cut(s) 55
Cac8I GCNNGC 1 cut(s) 119
CciI TCATGA 1 cut(s) 439
CfoI GCGC 3 cut(s) 295, 338, 436
Cfr13I GGNCC 2 cut(s) 167, 472
CviAII CATG 5 cut(s) 113, 118, 205, 271, 440
CviJI RGCY 5 cut(s) 169, 187, 265, 304, 500
CviKI_1 RGCY 5 cut(s) 169, 187, 265, 304, 500
DdeI CTNAG 3 cut(s) 101, 305, 321
DinI GGCGCC 1 cut(s) 294
DpnI GATC 4 cut(s) 10, 99, 269, 573
DpnII GATC 4 cut(s) 8, 97, 267, 571
DrdI GACNNNNNNGTC 1 cut(s) 452
DseDI GACNNNNNNGTC 1 cut(s) 452
Eam1104I CTCTTC 3 cut(s) 79, 543, 614
EarI CTCTTC 3 cut(s) 79, 543, 614
Eco130I CCWWGG 2 cut(s) 112, 204
Eco47I GGWCC 1 cut(s) 472
Eco47III AGCGCT 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 112, 204
EgeI GGCGCC 1 cut(s) 294
EheI GGCGCC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 112, 204
FaeI CATG 5 cut(s) 116, 121, 208, 274, 443
FaiI YATR 6 cut(s) 114, 119, 206, 272, 356, 441
FaqI GGGAC 1 cut(s) 111
FatI CATG 5 cut(s) 112, 117, 204, 270, 439
FbaI TGATCA 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 89, 507, 510
FokI GGATG 1 cut(s) 395
Fsp4HI GCNGC 3 cut(s) 89, 507, 510
FspBI CTAG 1 cut(s) 261
GlaI GCGC 3 cut(s) 294, 337, 435
GluI GCNGC 3 cut(s) 89, 507, 510
HaeII RGCGCY 2 cut(s) 296, 437
HaeIII GGCC 1 cut(s) 169
HapII CCGG 2 cut(s) 135, 173
HhaI GCGC 3 cut(s) 295, 338, 436
Hin1I GRCGYC 1 cut(s) 293
Hin1II CATG 5 cut(s) 116, 121, 208, 274, 443
Hin6I GCGC 3 cut(s) 293, 336, 434
HinP1I GCGC 3 cut(s) 293, 336, 434
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 5 cut(s) 80, 314, 453, 527, 603
HpaII CCGG 2 cut(s) 135, 173
HphI GGTGA 3 cut(s) 289, 470, 578
Hpy166II GTNNAC 2 cut(s) 397, 628
Hpy188I TCNGA 3 cut(s) 79, 196, 362
Hpy188III TCNNGA 4 cut(s) 54, 128, 250, 440
Hpy8I GTNNAC 2 cut(s) 397, 628
Hpy99I CGWCG 1 cut(s) 301
HpyAV CCTTC 2 cut(s) 137, 395
HpyCH4III ACNGT 3 cut(s) 215, 343, 539
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 109, 121, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 301, 506
HpyF3I CTNAG 3 cut(s) 101, 305, 321
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 293
Hsp92II CATG 5 cut(s) 116, 121, 208, 274, 443
HspAI GCGC 3 cut(s) 293, 336, 434
KasI GGCGCC 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 267
Kzo9I GATC 4 cut(s) 8, 97, 267, 571
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 1 cut(s) 431
LpnPI CCDG 4 cut(s) 34, 148, 186, 570
LweI GCATC 2 cut(s) 232, 336
MaeI CTAG 1 cut(s) 261
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 1 cut(s) 476
MalI GATC 4 cut(s) 10, 99, 269, 573
MboI GATC 4 cut(s) 8, 97, 267, 571
MboII GAAGA 5 cut(s) 66, 309, 375, 560, 601
MflI RGATCY 2 cut(s) 8, 97
MluCI AATT 1 cut(s) 593
Mly113I GGCGCC 1 cut(s) 293
MlyI GAGTC 3 cut(s) 462, 536, 612
MmeI TCCRAC 1 cut(s) 75
MseI TTAA 1 cut(s) 596
MslI CAYNNNNRTG 2 cut(s) 275, 540
MspI CCGG 2 cut(s) 135, 173
MspR9I CCNGG 1 cut(s) 135
MvnI CGCG 3 cut(s) 55, 237, 512
MwoI GCNNNNNNNGC 2 cut(s) 301, 506
NarI GGCGCC 1 cut(s) 293
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 2 cut(s) 112, 204
NdeII GATC 4 cut(s) 8, 97, 267, 571
NlaIII CATG 5 cut(s) 116, 121, 208, 274, 443
NlaIV GGNNCC 3 cut(s) 10, 28, 294
NmuCI GTSAC 1 cut(s) 476
NruI TCGCGA 1 cut(s) 55
NspI RCATGY 1 cut(s) 121
OliI CACNNNNGTG 1 cut(s) 540
PaeI GCATGC 1 cut(s) 121
PagI TCATGA 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PfeI GAWTC 2 cut(s) 80, 314
PkrI GCNGC 3 cut(s) 90, 508, 511
PleI GAGTC 3 cut(s) 461, 535, 611
PluTI GGCGCC 1 cut(s) 296
PpsI GAGTC 3 cut(s) 461, 535, 611
PspN4I GGNNCC 3 cut(s) 10, 28, 294
PspPI GGNCC 2 cut(s) 167, 472
PsuI RGATCY 2 cut(s) 8, 97
RruI TCGCGA 1 cut(s) 55
RseI CAYNNNNRTG 2 cut(s) 275, 540
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 1 cut(s) 596
SatI GCNGC 3 cut(s) 89, 507, 510
Sau3AI GATC 4 cut(s) 8, 97, 267, 571
Sau96I GGNCC 2 cut(s) 167, 472
SchI GAGTC 3 cut(s) 462, 536, 612
ScrFI CCNGG 1 cut(s) 135
SetI ASST 5 cut(s) 64, 148, 302, 474, 621
SfaNI GCATC 2 cut(s) 232, 336
SfoI GGCGCC 1 cut(s) 294
SinI GGWCC 1 cut(s) 472
SmiMI CAYNNNNRTG 2 cut(s) 275, 540
SphI GCATGC 1 cut(s) 121
Sse9I AATT 1 cut(s) 593
SsiI CCGC 7 cut(s) 88, 91, 275, 507, 510, 564, 579
SspDI GGCGCC 1 cut(s) 292
SspMI CTAG 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 2 cut(s) 112, 204
TaaI ACNGT 3 cut(s) 215, 343, 539
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 465
TasI AATT 1 cut(s) 593
TauI GCSGC 3 cut(s) 91, 509, 512
TfiI GAWTC 2 cut(s) 80, 314
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TscAI CASTG 1 cut(s) 128
TseFI GTSAC 1 cut(s) 476
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 1 cut(s) 428
TspGWI ACGGA 3 cut(s) 326, 446, 464
TspRI CASTG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 472
XceI RCATGY 1 cut(s) 121
XspI CTAG 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.