Rmu_sc0003238.1_g000025

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003238.1
OG0001959
Physical Location & Seq
Forward (+)
154697 .. 158719
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2214 bp
atgtggccggacttgatccaaaaggcaaaagatggaggccttgatgccgttgagacttatgtgttttggaatgcacatgaacctctgcgtaggcagtatgacttttcaggaaacctggacctggtgagattcattcagaccatccaaaaggcagggctctatgctgttcttcggattggaccatatgtttgtgcagaatggaattatggaggttttcctgtgtggctgcataacataccaggcatcaaattgagaacaaacaatgatatctataagaatgagatgcaaaactttacaactttgatagtggacatgatgcagcatgagaatctttttgcttcacaagggggacccattatacttgcccagattgaaaacgagtatgggaatgttatgcgctcgtatggcgatgaaggaaagaaatatatacaatggtgtgctgatttagctgaatcttaccagattggtgtaccatggattatgtgccaacaggaagatgctccacaatcaatgattaatacctgcaatggctggtattgtgatcaatttaagccttcatacaacactagcccgaagatgtggacagaaaattggactggatggttcaaggattggggcatgcaagatccacacagaactgcagaggatctcgccttttctgttgcacgattctttcagttaaatggcaccttccaaaactattatatgtaccatggaggaacaaatttcggtcgttcagcaggaggtccttacatcaccacatcatatgattatgatgcaccattagatgaatatggtaatttgaatcaacccaagtgggggcatcttaaagagcttcataaacatataagatcaatggagaaagttctgacttatggtgacgtaagacaggttgactatggaaacagctcgagtgtgacagagtacagctacagaggaaatcgatcctgtttcattagtaacgcaaacacaacaagcaatgtaatagtgaactttgagaataataagtattctgttcctgcttggtctgttactatccttcctgattgctatactgaagtctacaacactgcaaaggttaatgtgcaaagatcaataatgcagaaggtactaaatgaagccgatgctaacgatccagaggaaccttatggtttagtttggggttggagggctgagcactttacacacctcaaggaaaatggatcagttctgcatagcaatctcacgactaatcaactccttgatcagaaagtagtaacaaatgataccaggaccgaagtttctggacaacatttcaatcccttcactttagagagaaagattaagctaaagcatggaaagaatgatttatctctacttagtgtcacggttggcttgaaaaattatgatccactctttgatgaagtcaatgttgggattcatggtcctgttcaactgattggcaagtacaagaatggtactgaggtgacaaaggacttgagcaaaaatgagtggatatataaggttggacttgctggagaagaaaagggcctttaccaaataactggtcatgctgctaatttccactggcctacagataaacttccaacagatagaatgtttgtttggtacaagacaatcttcaaagctcctcttgggaccgatccagttgtggtggatttaatgggtttaggaaagggtcatgcttgggtgaacggtcgaagcattggcaggtattggacaagtcacactgcagatgaaaatggttgtacagcaaaatgcgactatcgtggaacttacagcaataagaagtgcttgactaattgtgggaaaccttcccaaagatggtaccatataccgcgatctttcctccaagatgacaataatgcactagttctctttgaggagtttggaggccagccgttcaatgttgaatttcagactgtaacagttgctaaagcttgcgcaaatgcttacgaaggtaatgtcttgcacttgtcatgccaaggaggaagagttttatccgatgttagattttccagcttcggtgatccacaaggcgcttgtggggcatccttcacaaaaggcgagtgtgaatcccccacagctttatcatacatccaaaaggcatgcattggcaaagcagaatgttttatcgatgtctctgaaatgactcttggaccaacaggctgcagagacatgaaccgccttgccgtcgaagtcgattgttcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Gal_Lectin Glyco_hydro_35

Protein Analysis

737

Amino Acids

83.5

Weight (kDa)

5.95

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 1 - 282 3.1e-109 Glycosyl hydrolases family 35
GHD PF17834 306 - 360 2.8e-21 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 535 - 622 1.4e-22 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 659 - 736 2e-13 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1945
Acc36I ACCTGC 2 cut(s) 530, 1701
Acc65I GGTACC 1 cut(s) 1827
AccB1I GGYRCC 2 cut(s) 686, 1827
AccI GTMKAC 1 cut(s) 1062
AccII CGCG 1 cut(s) 1840
AciI CCGC 2 cut(s) 1838, 2185
AclWI GGATC 9 cut(s) 10, 620, 654, 939, 1127, 1210, 1382, 1637, 2024
AcoI YGGCCR 1 cut(s) 5
AcsI RAATTY 2 cut(s) 724, 1913
AcuI CTGAAG 1 cut(s) 1077
AfaI GTAC 9 cut(s) 471, 710, 926, 1110, 1448, 1459, 1610, 1750, 1829
AfiI CCNNNNNNNGG 4 cut(s) 121, 818, 819, 1824
AgsI TTSAA 9 cut(s) 374, 607, 805, 1297, 1378, 1433, 1624, 1906, 1913
AhlI ACTAGT 1 cut(s) 1870
AjnI CCWGG 4 cut(s) 114, 120, 238, 1268
AjuI GAANNNNNNNTTGG 6 cut(s) 1395, 1427, 1588, 1620, 2139, 2171
AluBI AGCT 9 cut(s) 449, 835, 909, 930, 1327, 1628, 1940, 2022, 2087
AluI AGCT 9 cut(s) 449, 835, 909, 930, 1327, 1628, 1940, 2022, 2087
Alw21I GWGCWC 1 cut(s) 1179
Alw26I GTCTC 3 cut(s) 47, 2146, 2169
AlwI GGATC 9 cut(s) 10, 620, 654, 939, 1127, 1210, 1382, 1637, 2024
Ama87I CYCGRG 1 cut(s) 910
AoxI GGCC 5 cut(s) 5, 37, 1528, 1568, 1894
ApeKI GCWGC 4 cut(s) 226, 319, 1553, 2169
ApoI RAATTY 2 cut(s) 724, 1913
ArsI GACNNNNNNTTYG 6 cut(s) 171, 203, 1531, 1563, 1950, 1982
AseI ATTAAT 1 cut(s) 516
Asp718I GGTACC 1 cut(s) 1827
AspLEI GCGC 3 cut(s) 399, 1946, 2042
AspS9I GGNCC 9 cut(s) 118, 179, 350, 746, 1272, 1424, 1528, 1638, 2159
AsuHPI GGTGA 6 cut(s) 136, 748, 890, 1477, 1702, 2039
AvaI CYCGRG 1 cut(s) 910
AvaII GGWCC 8 cut(s) 118, 179, 350, 746, 1272, 1424, 1638, 2159
BaeI ACNNNNGTAYC 2 cut(s) 1449, 1482
BanI GGYRCC 2 cut(s) 686, 1827
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 1 cut(s) 1179
BbvI GCAGC 4 cut(s) 213, 331, 1540, 2156
BccI CCATC 4 cut(s) 26, 149, 594, 1818
BceAI ACGGC 3 cut(s) 32, 1885, 2177
BciT130I CCWGG 4 cut(s) 116, 122, 240, 1270
BclI TGATCA 2 cut(s) 541, 1243
BcoDI GTCTC 3 cut(s) 47, 2146, 2169
BcuI ACTAGT 1 cut(s) 1870
BfaI CTAG 2 cut(s) 567, 1871
BfmI CTRYAG 5 cut(s) 639, 931, 1572, 1731, 2170
BfoI RGCGCY 1 cut(s) 2043
BfuAI ACCTGC 2 cut(s) 530, 1701
BisI GCNGC 4 cut(s) 227, 320, 1554, 2170
BlpI GCTNAGC 1 cut(s) 1173
BlsI GCNGC 4 cut(s) 228, 321, 1555, 2171
Bme1390I CCNGG 4 cut(s) 116, 122, 240, 1270
Bme18I GGWCC 8 cut(s) 118, 179, 350, 746, 1272, 1424, 1638, 2159
BmeT110I CYCGRG 1 cut(s) 910
BmgT120I GGNCC 9 cut(s) 118, 179, 350, 746, 1272, 1424, 1528, 1638, 2159
BmiI GGNNCC 6 cut(s) 351, 352, 688, 1143, 1639, 1829
BmrFI CCNGG 4 cut(s) 116, 122, 240, 1270
BmsI GCATC 9 cut(s) 34, 252, 273, 306, 487, 766, 832, 1114, 2060
BpmI CTGGAG 1 cut(s) 1536
Bpu1102I GCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 2 cut(s) 1175, 1498
Bsa29I ATCGAT 2 cut(s) 943, 2136
BsaJI CCNNGG 3 cut(s) 473, 712, 1984
BsaXI ACNNNNNCTCC 2 cut(s) 1980, 2010
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 818, 819, 1824
Bse1I ACTGG 4 cut(s) 601, 1549, 1571, 1646
Bse3DI GCAATG 2 cut(s) 532, 985
BseBI CCWGG 4 cut(s) 116, 122, 240, 1270
BseCI ATCGAT 2 cut(s) 943, 2136
BseDI CCNNGG 3 cut(s) 473, 712, 1984
BseGI GGATG 4 cut(s) 141, 605, 2051, 2097
BseLI CCNNNNNNNGG 4 cut(s) 121, 818, 819, 1824
BseMI GCAATG 2 cut(s) 532, 985
BseMII CTCAG 2 cut(s) 1164, 1452
BseNI ACTGG 4 cut(s) 601, 1549, 1571, 1646
BseRI GAGGAG 2 cut(s) 1620, 1898
BseXI GCAGC 4 cut(s) 213, 331, 1540, 2156
BsgI GTGCAG 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 1840
Bsh1285I CGRYCG 2 cut(s) 733, 1699
BshFI GGCC 5 cut(s) 7, 39, 1530, 1570, 1896
BshNI GGYRCC 2 cut(s) 686, 1827
BshVI ATCGAT 2 cut(s) 943, 2136
BsiEI CGRYCG 2 cut(s) 733, 1699
BsiHKAI GWGCWC 1 cut(s) 1179
BsiHKCI CYCGRG 1 cut(s) 910
BsiSI CCGG 1 cut(s) 8
BslFI GGGAC 2 cut(s) 363, 1651
BslI CCNNNNNNNGG 4 cut(s) 121, 818, 819, 1824
BsmAI GTCTC 3 cut(s) 47, 2146, 2169
BsmFI GGGAC 2 cut(s) 363, 1651
BsmI GAATGC 1 cut(s) 76
BsnI GGCC 5 cut(s) 7, 39, 1530, 1570, 1896
BsoBI CYCGRG 1 cut(s) 910
Bsp1286I GDGCHC 2 cut(s) 159, 1179
Bsp1407I TGTACA 1 cut(s) 1748
Bsp1720I GCTNAGC 1 cut(s) 1173
Bsp19I CCATGG 2 cut(s) 473, 712
BspACI CCGC 2 cut(s) 1838, 2185
BspANI GGCC 5 cut(s) 7, 39, 1530, 1570, 1896
BspCNI CTCAG 2 cut(s) 1165, 1453
BspDI ATCGAT 2 cut(s) 943, 2136
BspFNI CGCG 1 cut(s) 1840
BspLI GGNNCC 6 cut(s) 351, 352, 688, 1143, 1639, 1829
BspMAI CTGCAG 3 cut(s) 643, 1735, 2174
BspMI ACCTGC 2 cut(s) 530, 1701
BspPI GGATC 9 cut(s) 10, 620, 654, 939, 1127, 1210, 1382, 1637, 2024
BspT107I GGYRCC 2 cut(s) 686, 1827
BsrDI GCAATG 2 cut(s) 532, 985
BsrGI TGTACA 1 cut(s) 1748
BsrI ACTGG 4 cut(s) 601, 1549, 1571, 1646
BssECI CCNNGG 3 cut(s) 473, 712, 1984
BssT1I CCWWGG 3 cut(s) 473, 712, 1984
Bst2UI CCWGG 4 cut(s) 116, 122, 240, 1270
Bst4CI ACNGT 4 cut(s) 1369, 1697, 1924, 1930
Bst6I CTCTTC 1 cut(s) 1987
BstAUI TGTACA 1 cut(s) 1748
BstC8I GCNNGC 4 cut(s) 620, 1898, 1942, 2110
BstDEI CTNAG 4 cut(s) 1173, 1358, 1461, 2211
BstDSI CCRYGG 2 cut(s) 473, 712
BstF5I GGATG 4 cut(s) 141, 605, 2051, 2097
BstFNI CGCG 1 cut(s) 1840
BstH2I RGCGCY 1 cut(s) 2043
BstHHI GCGC 3 cut(s) 399, 1946, 2042
BstMAI GTCTC 3 cut(s) 47, 2146, 2169
BstMCI CGRYCG 2 cut(s) 733, 1699
BstMWI GCNNNNNNNGC 3 cut(s) 405, 446, 2048
BstNI CCWGG 4 cut(s) 116, 122, 240, 1270
BstNSI RCATGY 2 cut(s) 622, 2112
BstSCI CCNGG 4 cut(s) 114, 120, 238, 1268
BstSFI CTRYAG 5 cut(s) 639, 931, 1572, 1731, 2170
BstUI CGCG 1 cut(s) 1840
BstV1I GCAGC 4 cut(s) 213, 331, 1540, 2156
BstX2I RGATCY 2 cut(s) 625, 646
BstXI CCANNNNNNTGG 1 cut(s) 1544
BstYI RGATCY 2 cut(s) 625, 646
Bsu15I ATCGAT 2 cut(s) 943, 2136
BsuRI GGCC 5 cut(s) 7, 39, 1530, 1570, 1896
BsuTUI ATCGAT 2 cut(s) 943, 2136
BtgI CCRYGG 2 cut(s) 473, 712
BtgZI GCGATG 1 cut(s) 423
BtsCI GGATG 4 cut(s) 141, 605, 2051, 2097
BtsI GCAGTG 2 cut(s) 1068, 1728
BtsIMutI CAGTG 3 cut(s) 1068, 1564, 1728
BveI ACCTGC 2 cut(s) 530, 1701
Cac8I GCNNGC 4 cut(s) 620, 1898, 1942, 2110
CfoI GCGC 3 cut(s) 399, 1946, 2042
Cfr13I GGNCC 9 cut(s) 118, 179, 350, 746, 1272, 1424, 1528, 1638, 2159
ClaI ATCGAT 2 cut(s) 943, 2136
CsiI ACCWGGT 1 cut(s) 120
Csp6I GTAC 9 cut(s) 470, 709, 925, 1109, 1447, 1458, 1609, 1749, 1828
CspCI CAANNNNNGTGG 2 cut(s) 797, 832
CviQI GTAC 9 cut(s) 470, 709, 925, 1109, 1447, 1458, 1609, 1749, 1828
DdeI CTNAG 4 cut(s) 1173, 1358, 1461, 2211
EaeI YGGCCR 1 cut(s) 5
Eam1104I CTCTTC 1 cut(s) 1987
EarI CTCTTC 1 cut(s) 1987
Eco130I CCWWGG 3 cut(s) 473, 712, 1984
Eco147I AGGCCT 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 159
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 8 cut(s) 118, 179, 350, 746, 1272, 1424, 1638, 2159
Eco57I CTGAAG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 910
EcoO109I RGGNCCY 3 cut(s) 350, 746, 1528
EcoRII CCWGG 4 cut(s) 114, 120, 238, 1268
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 3 cut(s) 473, 712, 1984
EcoT22I ATGCAT 1 cut(s) 2114
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 3 cut(s) 473, 712, 1984
FalI AAGNNNNNCTT 6 cut(s) 1604, 1636, 1617, 1649, 1778, 1810
FaqI GGGAC 2 cut(s) 363, 1651
FauNDI CATATG 2 cut(s) 184, 766
FbaI TGATCA 2 cut(s) 541, 1243
FblI GTMKAC 1 cut(s) 1062
Fnu4HI GCNGC 4 cut(s) 227, 320, 1554, 2170
FokI GGATG 4 cut(s) 128, 612, 2038, 2084
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 227, 320, 1554, 2170
FspBI CTAG 2 cut(s) 567, 1871
FspI TGCGCA 1 cut(s) 1945
GlaI GCGC 3 cut(s) 398, 1945, 2041
GluI GCNGC 4 cut(s) 227, 320, 1554, 2170
GsuI CTGGAG 1 cut(s) 1536
HaeII RGCGCY 1 cut(s) 2043
HaeIII GGCC 5 cut(s) 7, 39, 1530, 1570, 1896
HapII CCGG 1 cut(s) 8
HhaI GCGC 3 cut(s) 399, 1946, 2042
Hin6I GCGC 3 cut(s) 397, 1944, 2040
HinP1I GCGC 3 cut(s) 397, 1944, 2040
HincII GTYRAC 1 cut(s) 895
HindII GTYRAC 1 cut(s) 895
HindIII AAGCTT 1 cut(s) 1938
HinfI GANTC 8 cut(s) 129, 328, 452, 669, 805, 1417, 2075, 2152
HpaII CCGG 1 cut(s) 8
HphI GGTGA 6 cut(s) 136, 748, 890, 1477, 1702, 2039
Hpy166II GTNNAC 7 cut(s) 310, 470, 582, 895, 991, 1063, 1693
Hpy188I TCNGA 7 cut(s) 138, 174, 870, 1248, 1920, 2005, 2146
Hpy188III TCNNGA 5 cut(s) 108, 1043, 1136, 1225, 1284
Hpy8I GTNNAC 7 cut(s) 310, 470, 582, 895, 991, 1063, 1693
Hpy99I CGWCG 1 cut(s) 2198
HpyAV CCTTC 9 cut(s) 407, 564, 700, 1049, 1099, 1312, 1824, 1952, 2065
HpyCH4III ACNGT 4 cut(s) 1369, 1697, 1924, 1930
HpyCH4IV ACGT 1 cut(s) 882
HpyF10VI GCNNNNNNNGC 3 cut(s) 405, 446, 2048
HpyF3I CTNAG 4 cut(s) 1173, 1358, 1461, 2211
HpySE526I ACGT 1 cut(s) 882
HspAI GCGC 3 cut(s) 397, 1944, 2040
KflI GGGWCCC 1 cut(s) 350
KpnI GGTACC 1 cut(s) 1831
Ksp22I TGATCA 2 cut(s) 541, 1243
LmnI GCTCC 2 cut(s) 505, 1633
Lsp1109I GCAGC 4 cut(s) 213, 331, 1540, 2156
LweI GCATC 9 cut(s) 34, 252, 273, 306, 487, 766, 832, 1114, 2060
MabI ACCWGGT 1 cut(s) 120
MaeI CTAG 2 cut(s) 567, 1871
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 9 cut(s) 878, 916, 959, 1030, 1255, 1363, 1465, 1724, 1924
MboII GAAGA 6 cut(s) 161, 506, 586, 1532, 1612, 2004
MflI RGATCY 2 cut(s) 625, 646
MhlI GDGCHC 2 cut(s) 159, 1179
MlyI GAGTC 1 cut(s) 2146
MmeI TCCRAC 3 cut(s) 1145, 1486, 1610
Mph1103I ATGCAT 1 cut(s) 2114
MseI TTAA 7 cut(s) 516, 549, 680, 828, 1080, 1323, 1661
MspI CCGG 1 cut(s) 8
MspR9I CCNGG 4 cut(s) 116, 122, 240, 1270
Mva1269I GAATGC 1 cut(s) 76
MvaI CCWGG 4 cut(s) 116, 122, 240, 1270
MvnI CGCG 1 cut(s) 1840
MwoI GCNNNNNNNGC 3 cut(s) 405, 446, 2048
NcoI CCATGG 2 cut(s) 473, 712
NdeI CATATG 2 cut(s) 184, 766
NlaIV GGNNCC 6 cut(s) 351, 352, 688, 1143, 1639, 1829
NmuCI GTSAC 5 cut(s) 878, 916, 1363, 1465, 1724
NsbI TGCGCA 1 cut(s) 1945
NsiI ATGCAT 1 cut(s) 2114
NspI RCATGY 2 cut(s) 622, 2112
PaeI GCATGC 2 cut(s) 622, 2112
PaeR7I CTCGAG 1 cut(s) 910
PceI AGGCCT 1 cut(s) 39
PctI GAATGC 1 cut(s) 76
PfeI GAWTC 7 cut(s) 129, 328, 452, 669, 805, 1417, 2075
PkrI GCNGC 4 cut(s) 228, 321, 1555, 2171
PleI GAGTC 1 cut(s) 2146
PpsI GAGTC 1 cut(s) 2146
PpuMI RGGWCCY 2 cut(s) 350, 746
PshBI ATTAAT 1 cut(s) 516
Psp5II RGGWCCY 2 cut(s) 350, 746
Psp6I CCWGG 4 cut(s) 114, 120, 238, 1268
PspGI CCWGG 4 cut(s) 114, 120, 238, 1268
PspN4I GGNNCC 6 cut(s) 351, 352, 688, 1143, 1639, 1829
PspPI GGNCC 9 cut(s) 118, 179, 350, 746, 1272, 1424, 1528, 1638, 2159
PspPPI RGGWCCY 2 cut(s) 350, 746
PspXI VCTCGAGB 1 cut(s) 910
PstI CTGCAG 3 cut(s) 643, 1735, 2174
PsuI RGATCY 2 cut(s) 625, 646
RsaI GTAC 9 cut(s) 471, 710, 926, 1110, 1448, 1459, 1610, 1750, 1829
RsaNI GTAC 9 cut(s) 470, 709, 925, 1109, 1447, 1458, 1609, 1749, 1828
SaqAI TTAA 7 cut(s) 516, 549, 680, 828, 1080, 1323, 1661
SatI GCNGC 4 cut(s) 227, 320, 1554, 2170
Sau96I GGNCC 9 cut(s) 118, 179, 350, 746, 1272, 1424, 1528, 1638, 2159
SchI GAGTC 1 cut(s) 2146
ScrFI CCNGG 4 cut(s) 116, 122, 240, 1270
SduI GDGCHC 2 cut(s) 159, 1179
SexAI ACCWGGT 1 cut(s) 120
SfaNI GCATC 9 cut(s) 34, 252, 273, 306, 487, 766, 832, 1114, 2060
SfcI CTRYAG 5 cut(s) 639, 931, 1572, 1731, 2170
Sfr274I CTCGAG 1 cut(s) 910
SinI GGWCC 8 cut(s) 118, 179, 350, 746, 1272, 1424, 1638, 2159
SlaI CTCGAG 1 cut(s) 910
SmlI CTYRAG 3 cut(s) 910, 1190, 1477
SmoI CTYRAG 3 cut(s) 910, 1190, 1477
SpeI ACTAGT 1 cut(s) 1870
SphI GCATGC 2 cut(s) 622, 2112
SseBI AGGCCT 1 cut(s) 39
SsiI CCGC 2 cut(s) 1838, 2185
SspMI CTAG 2 cut(s) 567, 1871
StuI AGGCCT 1 cut(s) 39
StyD4I CCNGG 4 cut(s) 114, 120, 238, 1268
StyI CCWWGG 3 cut(s) 473, 712, 1984
TaaI ACNGT 4 cut(s) 1369, 1697, 1924, 1930
TaiI ACGT 1 cut(s) 885
TaqI TCGA 6 cut(s) 911, 943, 1699, 2136, 2196, 2202
TaqII GACCGA 3 cut(s) 719, 1289, 1655
TatI WGTACW 3 cut(s) 924, 1446, 1748
TfiI GAWTC 7 cut(s) 129, 328, 452, 669, 805, 1417, 2075
Tru1I TTAA 7 cut(s) 516, 549, 680, 828, 1080, 1323, 1661
Tru9I TTAA 7 cut(s) 516, 549, 680, 828, 1080, 1323, 1661
TscAI CASTG 3 cut(s) 1075, 1571, 1735
TseFI GTSAC 5 cut(s) 878, 916, 1363, 1465, 1724
TseI GCWGC 4 cut(s) 226, 319, 1553, 2169
Tsp45I GTSAC 5 cut(s) 878, 916, 1363, 1465, 1724
TspRI CASTG 3 cut(s) 1075, 1571, 1735
VpaK11BI GGWCC 8 cut(s) 118, 179, 350, 746, 1272, 1424, 1638, 2159
VspI ATTAAT 1 cut(s) 516
XapI RAATTY 2 cut(s) 724, 1913
XceI RCATGY 2 cut(s) 622, 2112
XhoI CTCGAG 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 1062
XspI CTAG 2 cut(s) 567, 1871
Zsp2I ATGCAT 1 cut(s) 2114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.