Rmu_sc0006005.1_g000003

Nuclease-related domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006005.1
OG0003421
Physical Location & Seq
Reverse (-)
4961 .. 7601
2641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1137 bp
atgtggttcgagatcctctgcggcctggtgatttacagactgtacaagttcctcttctccgacggcgacgacgtcttggatgtcgagacctctgattctaccgctctcttcgccgtcgccgacaggctcgctaagctctacgatggcgccaaggtctgcaacatgtggttcgagatcatctgcggcctggtgatttacagactgtacaagttgttcttctccgacggcgacgacgtcttggatgtcgagacctctgattctaccgctctcttcgccgtcgccgacaggctcgctaagctctacgatggcgccaaggtctacgttggcctccgggtccccgacgccgataccgcttcccctcagcacatcgatttggttctcctcaacaaaggggaggccgtggtgatagccgtgaagaacttgtcaggattcatctccgtcaagcccgatggcagctgggtttctgagagcctcactaaacacaaaatagagcagcatcctgatcctctggcggagacaaaaagacaagcttcaattcttgagtcctatcttgaacaaagaggagctgctttaccagaaggatatttgtcttgcaaagtaatactttccaatccaaaagtatgtaccattcagtcaagcagtttcccaccagaaatcattacttatgaccagtgggtacaaatgaaaccagaaccaaaaagtatgttttctggttggatgaagggcgcattccgtggtggaaagaaagagatgcaagaatctattcatcagaagcttaatttcattcttagtacagcaccaatgtgggatagattggagcttaaaggtaacaagtatgttcttggggaatttctggaattcaagggtaagcaggaagatgttcaggctttgaggcatattaaaagatcaaaaattagccgccttgttgtccaaaagacaagcatgcttggatttgccccttcaaggctccaagttctgtatactcgtcgtgattaccgaagtgaaggagcttcagcttctgagtggaaagaagtaaatgtaaggtcaagcacggaggttgtctttcagcttgacaattcttcaagagtccgcaagtttaagctctcctcaatttgttctatgtctctgagtgcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NERD

Protein Analysis

378

Amino Acids

42.64

Weight (kDa)

8.07

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NERD PF08378 91 - 197 3.6e-12 Nuclease-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 75, 237
AccB1I GGYRCC 2 cut(s) 146, 308
AccBSI CCGCTC 2 cut(s) 104, 266
AccI GTMKAC 2 cut(s) 318, 980
AciI CCGC 8 cut(s) 21, 102, 183, 264, 351, 512, 919, 1090
AclWI GGATC 2 cut(s) 7, 497
AcsI RAATTY 2 cut(s) 848, 857
AcuI CTGAAG 1 cut(s) 996
AcyI GRCGYC 5 cut(s) 72, 147, 234, 309, 342
AfaI GTAC 5 cut(s) 44, 206, 625, 678, 793
AfiI CCNNNNNNNGG 1 cut(s) 963
AflIII ACRYGT 1 cut(s) 162
AgsI TTSAA 5 cut(s) 534, 554, 862, 963, 1083
AjnI CCWGG 2 cut(s) 24, 186
AleI CACNNNNGTG 1 cut(s) 802
Alw26I GTCTC 4 cut(s) 80, 242, 509, 1128
AlwI GGATC 2 cut(s) 7, 497
AlwNI CAGNNNCTG 1 cut(s) 1019
AoxI GGCC 4 cut(s) 22, 184, 325, 396
ApeKI GCWGC 3 cut(s) 453, 493, 566
ApoI RAATTY 2 cut(s) 848, 857
Asp700I GAANNNNTTC 2 cut(s) 762, 879
AspLEI GCGC 3 cut(s) 149, 311, 728
AspS9I GGNCC 1 cut(s) 334
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 3 cut(s) 40, 202, 415
AvaII GGWCC 1 cut(s) 334
BanI GGYRCC 2 cut(s) 146, 308
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 3 cut(s) 465, 505, 553
BccI CCATC 3 cut(s) 137, 299, 443
BceAI ACGGC 6 cut(s) 79, 98, 241, 260, 383, 395
BciT130I CCWGG 2 cut(s) 26, 188
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 4 cut(s) 80, 242, 509, 1128
BfoI RGCGCY 2 cut(s) 150, 312
BisI GCNGC 6 cut(s) 22, 184, 454, 494, 567, 919
BlpI GCTNAGC 2 cut(s) 132, 294
BlsI GCNGC 6 cut(s) 23, 185, 455, 495, 568, 920
Bme1390I CCNGG 3 cut(s) 26, 188, 332
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 5 cut(s) 148, 310, 335, 336, 968
BmrFI CCNGG 3 cut(s) 26, 188, 332
BmsI GCATC 2 cut(s) 505, 741
Bpu10I CCTNAGC 1 cut(s) 360
Bpu1102I GCTNAGC 2 cut(s) 132, 294
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 332
Bsa29I ATCGAT 1 cut(s) 369
BsaHI GRCGYC 5 cut(s) 72, 147, 234, 309, 342
BsaI GGTCTC 2 cut(s) 80, 242
BsaJI CCNNGG 4 cut(s) 150, 312, 399, 733
Bsc4I CCNNNNNNNGG 1 cut(s) 963
Bse1I ACTGG 1 cut(s) 670
BseBI CCWGG 2 cut(s) 26, 188
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 4 cut(s) 150, 312, 399, 733
BseGI GGATG 4 cut(s) 85, 247, 496, 723
BseLI CCNNNNNNNGG 1 cut(s) 963
BseMII CTCAG 4 cut(s) 374, 456, 1011, 1118
BseNI ACTGG 1 cut(s) 670
BseRI GAGGAG 3 cut(s) 371, 576, 1096
BseXI GCAGC 3 cut(s) 465, 505, 553
BseYI CCCAGC 1 cut(s) 456
BshFI GGCC 4 cut(s) 24, 186, 327, 398
BshNI GGYRCC 2 cut(s) 146, 308
BshVI ATCGAT 1 cut(s) 369
BsiSI CCGG 1 cut(s) 331
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 963
BsmAI GTCTC 4 cut(s) 80, 242, 509, 1128
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 4 cut(s) 24, 186, 327, 398
Bso31I GGTCTC 2 cut(s) 80, 242
Bsp1407I TGTACA 2 cut(s) 42, 204
Bsp143I GATC 4 cut(s) 12, 174, 502, 905
Bsp1720I GCTNAGC 2 cut(s) 132, 294
BspACI CCGC 8 cut(s) 21, 102, 183, 264, 351, 512, 919, 1090
BspANI GGCC 4 cut(s) 24, 186, 327, 398
BspCNI CTCAG 4 cut(s) 373, 457, 1012, 1119
BspDI ATCGAT 1 cut(s) 369
BspLI GGNNCC 5 cut(s) 148, 310, 335, 336, 968
BspPI GGATC 2 cut(s) 7, 497
BspT107I GGYRCC 2 cut(s) 146, 308
BspTNI GGTCTC 2 cut(s) 80, 242
BsrBI CCGCTC 2 cut(s) 104, 266
BsrGI TGTACA 2 cut(s) 42, 204
BsrI ACTGG 1 cut(s) 670
BssECI CCNNGG 4 cut(s) 150, 312, 399, 733
BssMI GATC 4 cut(s) 12, 174, 502, 905
BssNAI GTATAC 1 cut(s) 981
BssNI GRCGYC 5 cut(s) 72, 147, 234, 309, 342
BssT1I CCWWGG 2 cut(s) 150, 312
Bst1107I GTATAC 1 cut(s) 981
Bst2UI CCWGG 2 cut(s) 26, 188
Bst4CI ACNGT 2 cut(s) 42, 204
Bst6I CTCTTC 3 cut(s) 59, 113, 275
BstACI GRCGYC 5 cut(s) 72, 147, 234, 309, 342
BstAUI TGTACA 2 cut(s) 42, 204
BstC8I GCNNGC 3 cut(s) 129, 291, 944
BstDEI CTNAG 7 cut(s) 132, 294, 360, 465, 788, 1020, 1127
BstDSI CCRYGG 2 cut(s) 399, 733
BstF5I GGATG 4 cut(s) 85, 247, 496, 723
BstH2I RGCGCY 2 cut(s) 150, 312
BstHHI GCGC 3 cut(s) 149, 311, 728
BstKTI GATC 4 cut(s) 15, 177, 505, 908
BstMAI GTCTC 4 cut(s) 80, 242, 509, 1128
BstMBI GATC 4 cut(s) 12, 174, 502, 905
BstMWI GCNNNNNNNGC 5 cut(s) 110, 133, 272, 295, 350
BstNI CCWGG 2 cut(s) 26, 188
BstNSI RCATGY 2 cut(s) 166, 946
BstSCI CCNGG 3 cut(s) 24, 186, 330
BstV1I GCAGC 3 cut(s) 465, 505, 553
BstX2I RGATCY 1 cut(s) 12
BstYI RGATCY 1 cut(s) 12
BstZ17I GTATAC 1 cut(s) 981
Bsu15I ATCGAT 1 cut(s) 369
BsuRI GGCC 4 cut(s) 24, 186, 327, 398
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 2 cut(s) 399, 733
BtsCI GGATG 4 cut(s) 85, 247, 496, 723
BtsIMutI CAGTG 1 cut(s) 677
Cac8I GCNNGC 3 cut(s) 129, 291, 944
CaiI CAGNNNCTG 1 cut(s) 1019
CfoI GCGC 3 cut(s) 149, 311, 728
Cfr13I GGNCC 1 cut(s) 334
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 1 cut(s) 350
Csp6I GTAC 5 cut(s) 43, 205, 624, 677, 792
CviAII CATG 2 cut(s) 163, 943
CviQI GTAC 5 cut(s) 43, 205, 624, 677, 792
DdeI CTNAG 7 cut(s) 132, 294, 360, 465, 788, 1020, 1127
DinI GGCGCC 2 cut(s) 148, 310
DpnI GATC 4 cut(s) 14, 176, 504, 907
DpnII GATC 4 cut(s) 12, 174, 502, 905
Eam1104I CTCTTC 3 cut(s) 59, 113, 275
EarI CTCTTC 3 cut(s) 59, 113, 275
EciI GGCGGA 1 cut(s) 527
Eco130I CCWWGG 2 cut(s) 150, 312
Eco31I GGTCTC 2 cut(s) 80, 242
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 996
EcoO109I RGGNCCY 1 cut(s) 334
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 2 cut(s) 24, 186
EcoT14I CCWWGG 2 cut(s) 150, 312
EgeI GGCGCC 2 cut(s) 148, 310
EheI GGCGCC 2 cut(s) 148, 310
ErhI CCWWGG 2 cut(s) 150, 312
FaeI CATG 2 cut(s) 166, 946
FaiI YATR 9 cut(s) 164, 622, 666, 704, 837, 897, 944, 981, 1121
FalI AAGNNNNNCTT 8 cut(s) 38, 70, 200, 232, 514, 546, 588, 620
FaqI GGGAC 1 cut(s) 320
FatI CATG 2 cut(s) 162, 942
FblI GTMKAC 2 cut(s) 318, 980
Fnu4HI GCNGC 6 cut(s) 22, 184, 454, 494, 567, 919
FokI GGATG 4 cut(s) 92, 254, 483, 730
Fsp4HI GCNGC 6 cut(s) 22, 184, 454, 494, 567, 919
GlaI GCGC 3 cut(s) 148, 310, 727
GluI GCNGC 6 cut(s) 22, 184, 454, 494, 567, 919
GsaI CCCAGC 1 cut(s) 460
HaeII RGCGCY 2 cut(s) 150, 312
HaeIII GGCC 4 cut(s) 24, 186, 327, 398
HapII CCGG 1 cut(s) 331
HgaI GACGC 1 cut(s) 350
HhaI GCGC 3 cut(s) 149, 311, 728
Hin1I GRCGYC 5 cut(s) 72, 147, 234, 309, 342
Hin1II CATG 2 cut(s) 166, 946
Hin6I GCGC 3 cut(s) 147, 309, 726
HinP1I GCGC 3 cut(s) 147, 309, 726
HindIII AAGCTT 2 cut(s) 528, 773
HinfI GANTC 6 cut(s) 95, 257, 429, 542, 758, 1086
HpaII CCGG 1 cut(s) 331
HphI GGTGA 3 cut(s) 40, 202, 415
Hpy166II GTNNAC 2 cut(s) 319, 981
Hpy188I TCNGA 8 cut(s) 61, 94, 223, 256, 466, 771, 1021, 1128
Hpy8I GTNNAC 2 cut(s) 319, 981
HpyAV CCTTC 4 cut(s) 572, 715, 969, 998
HpyCH4III ACNGT 2 cut(s) 42, 204
HpyCH4IV ACGT 3 cut(s) 72, 234, 321
HpyCH4V TGCA 3 cut(s) 159, 594, 754
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 133, 272, 295, 350
HpyF3I CTNAG 7 cut(s) 132, 294, 360, 465, 788, 1020, 1127
HpySE526I ACGT 3 cut(s) 72, 234, 321
Hsp92I GRCGYC 5 cut(s) 72, 147, 234, 309, 342
Hsp92II CATG 2 cut(s) 166, 946
HspAI GCGC 3 cut(s) 147, 309, 726
KasI GGCGCC 2 cut(s) 146, 308
KflI GGGWCCC 1 cut(s) 334
Kzo9I GATC 4 cut(s) 12, 174, 502, 905
LmnI GCTCC 4 cut(s) 563, 817, 972, 1007
Lsp1109I GCAGC 3 cut(s) 465, 505, 553
LweI GCATC 2 cut(s) 505, 741
MaeII ACGT 3 cut(s) 72, 234, 321
MaeIII GTNAC 1 cut(s) 827
MalI GATC 4 cut(s) 14, 176, 504, 907
MbiI CCGCTC 2 cut(s) 104, 266
MboI GATC 4 cut(s) 12, 174, 502, 905
MboII GAAGA 7 cut(s) 46, 100, 208, 262, 427, 887, 1071
MflI RGATCY 1 cut(s) 12
MluCI AATT 7 cut(s) 534, 778, 848, 857, 912, 1075, 1110
Mly113I GGCGCC 2 cut(s) 147, 309
MlyI GAGTC 2 cut(s) 551, 1095
MmeI TCCRAC 3 cut(s) 84, 246, 695
MroXI GAANNNNTTC 2 cut(s) 762, 879
MseI TTAA 5 cut(s) 777, 822, 900, 1098, 1135
MslI CAYNNNNRTG 1 cut(s) 802
MspA1I CMGCKG 1 cut(s) 456
MspI CCGG 1 cut(s) 331
MspR9I CCNGG 3 cut(s) 26, 188, 332
Mva1269I GAATGC 1 cut(s) 728
MvaI CCWGG 2 cut(s) 26, 188
MwoI GCNNNNNNNGC 5 cut(s) 110, 133, 272, 295, 350
NarI GGCGCC 2 cut(s) 147, 309
NciI CCSGG 1 cut(s) 332
NdeII GATC 4 cut(s) 12, 174, 502, 905
NlaIII CATG 2 cut(s) 166, 946
NlaIV GGNNCC 5 cut(s) 148, 310, 335, 336, 968
NspI RCATGY 2 cut(s) 166, 946
OliI CACNNNNGTG 1 cut(s) 802
PaeI GCATGC 1 cut(s) 946
PciI ACATGT 1 cut(s) 162
PcsI WCGNNNNNNNCGW 5 cut(s) 69, 117, 231, 279, 994
PctI GAATGC 1 cut(s) 728
PdmI GAANNNNTTC 2 cut(s) 762, 879
PfeI GAWTC 4 cut(s) 95, 257, 429, 758
PflFI GACNNNGTC 2 cut(s) 71, 233
PkrI GCNGC 6 cut(s) 23, 185, 455, 495, 568, 920
PleI GAGTC 2 cut(s) 550, 1094
PluTI GGCGCC 2 cut(s) 150, 312
PpsI GAGTC 2 cut(s) 550, 1094
PpuMI RGGWCCY 1 cut(s) 334
PscI ACATGT 1 cut(s) 162
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 24, 186
PspFI CCCAGC 1 cut(s) 456
PspGI CCWGG 2 cut(s) 24, 186
PspN4I GGNNCC 5 cut(s) 148, 310, 335, 336, 968
PspPI GGNCC 1 cut(s) 334
PspPPI RGGWCCY 1 cut(s) 334
PsrI GAACNNNNNNTAC 2 cut(s) 197, 229
PstNI CAGNNNCTG 1 cut(s) 1019
PsuI RGATCY 1 cut(s) 12
PsyI GACNNNGTC 2 cut(s) 71, 233
PvuII CAGCTG 1 cut(s) 456
RsaI GTAC 5 cut(s) 44, 206, 625, 678, 793
RsaNI GTAC 5 cut(s) 43, 205, 624, 677, 792
RseI CAYNNNNRTG 1 cut(s) 802
SaqAI TTAA 5 cut(s) 777, 822, 900, 1098, 1135
SatI GCNGC 6 cut(s) 22, 184, 454, 494, 567, 919
Sau3AI GATC 4 cut(s) 12, 174, 502, 905
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 2 cut(s) 551, 1095
ScrFI CCNGG 3 cut(s) 26, 188, 332
SfaNI GCATC 2 cut(s) 505, 741
SfoI GGCGCC 2 cut(s) 148, 310
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 802
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SphI GCATGC 1 cut(s) 946
Sse9I AATT 7 cut(s) 534, 778, 848, 857, 912, 1075, 1110
SsiI CCGC 8 cut(s) 21, 102, 183, 264, 351, 512, 919, 1090
SspDI GGCGCC 2 cut(s) 146, 308
StyD4I CCNGG 3 cut(s) 24, 186, 330
StyI CCWWGG 2 cut(s) 150, 312
TaaI ACNGT 2 cut(s) 42, 204
TaiI ACGT 3 cut(s) 75, 237, 324
TaqI TCGA 5 cut(s) 9, 84, 171, 246, 369
TasI AATT 7 cut(s) 534, 778, 848, 857, 912, 1075, 1110
TatI WGTACW 3 cut(s) 42, 204, 791
TauI GCSGC 3 cut(s) 24, 186, 921
TfiI GAWTC 4 cut(s) 95, 257, 429, 758
Tru1I TTAA 5 cut(s) 777, 822, 900, 1098, 1135
Tru9I TTAA 5 cut(s) 777, 822, 900, 1098, 1135
TscAI CASTG 1 cut(s) 677
TseI GCWGC 3 cut(s) 453, 493, 566
TspDTI ATGAA 5 cut(s) 421, 698, 734, 755, 772
TspGWI ACGGA 3 cut(s) 427, 722, 1067
TspRI CASTG 1 cut(s) 677
Tth111I GACNNNGTC 2 cut(s) 71, 233
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 2 cut(s) 848, 857
XceI RCATGY 2 cut(s) 166, 946
XmiI GTMKAC 2 cut(s) 318, 980
XmnI GAANNNNTTC 2 cut(s) 762, 879
ZraI GACGTC 2 cut(s) 73, 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.