Rroxscaffold_1G00023120

Protein FAR1-RELATED SEQUENCE 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002145
Physical Location & Seq
Reverse (-)
28619258 .. 28621996
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00023120.1

Sequence Viewer

Length: 2127 bp
ATGGAAAATAAAGACGATGAATCTTGTTATCAGCATTGTAGTAACACTGAGAATGAGCAAAGAGGATGTGAAGATGATGCAATTGATGGTGAGACTAATGAGGAACCTGAATATGATACTCAAGAGGCTAGTAGCGATACTCAAGTGCCCAGTGATGATGAACACGTGGATTCTGGTTTAGAAGGTTTATGTATCTTGGATGGTAAGAAGCAAGCAAGAGGTGAGAATATCCCAGTTCTTGGTATGAGTTTTGAAAGTGATCAAGATGCATATGACTATTATAATTCATATGCTAGAGTTGTTGGGTTTAGCGTGCGAAGGCTGAGAAGCAACAAATATAAGCATACTAATGTGACATGGAAAAGAGAATTTTGTTGCTCTTGTGAAGGATTTTATACAAAAAAGAACACTCCAGAGAAGAAAAGAGAAGAAAGAAGATTCGGATGTAAGGCAATGCTTCAGATCAAACTAAATGAAGATGGGAAATTTGTTGTCACAAAGTTTGAAGCTGAGCGTACCCATGATCTTGTTCCTGAATCAAGTTCACATGTCTTAAGGTCACAAAGAACCATAGAACCTTCTCAAGGTGGTTTGATGAATCAAATGCACTTTGCAGGGTTCAAACCATCACAGATCTTTTCATACCTGACCGTCGAAGCAGGAGGACCGGAAAATCTAAATTTCCTTCAAACCGATTGCAATAACTTCATTCAAAGAAGGCGGGCAAAGTTTTTGAAAAAAGGTGATGCTCAATGTTTGCTTGATTACTTTAAGAAGAAGCAAATGGAGGATAAGTCATTTTTTTATGCAATTCGAACAAACATCGACAATGAAATATGTGGTTGTTTTTTTTGTGATGGAAAATCAAGGAGTGATTATGCTATATTTGGTGATGCGGTTGTCTTTGATACAACCTTCAAAACCAACAAGTATGACATGGTTTGTGCTCGAATTGTTGGAGTTAATCATCATGGTCAAACAATTCTATTTGGCTGTGGGTTATTAGATGGGGAGACCACAGAGGCATGCAAATGGTTCTTTGATGTTTTCCTACAAGCTATGGGAGGAAAAAAACCAAAGACAATATTTAAAGATCAAGCTGCATCGATTGCTAGTGCAATTAGGGAAGTACTACCTAATTCTCACCATCGTCTTTGTTTGTGGCACATATATCAAAATGCTGCAAAACACTTGAGTCAAGTCTTTACGAAGTGCTCCACATTTTCACAAGCTTTCAAAAGTTGCATATATGATCCAGAGAGTGTTGAAGAGTTTGAATCAAGTTGGAAAGATCTACTTGATTACTATGATTTGAAAGAAAATGAGTGGCTAAAAGACTTGTATAACTTGCGTGAGAAGTGGGCACAAATTTATGGCCGACGCAACTTTTGTGCAGGTATGACAACAACTCAGAGAAGTGAAAGTATAAACAAGACTTTGAAAAAGTTCTTTTATAAGAACCTTATTCTTTGTGAGTGGGTGGTACACTATGAGCGTGCTTTAGTTGATCGAAGAGAAAAGGAGAGGCTCGCGGAAGTTGCAACTACGCAAAGAAAGCGAAAACTTCTATCTAATTGGAAAGTGGAGGTTGAAGCAGCAAAGATGTACACAAAAGCGAGTTTCAATTGTTTTCAAGAAGAGTTTCGAAAATGTTTGGACTTGATACTGGAATTAGAGAGTGATGACGGGAGAATAGAATCATATGTGGTTCAAAGACCAGGGAATCCAAACTTGAGAAGTCACTTGTCTCATATAGCACAAAGAATTGTAGCAAAAGGTGCAAAGGATAAGCAATCATCCACCTTGGTAGAGTCTAAATTGTTGGAGTTGGAGGCAGAATTGGATGGCAATTTATCCATTGGACAAGAGCATGAAACAAATGGTGATATAGATTCAGAAGATCAAGTGAATGAAAATAATGCAAATCTGGTTTTGCGTGATCCAAAAGTTAAGAGGCGTAGAAGGCGTGGCAAAGGTACGAGAAATATTGATTTGGGATCTAAAAAGCAATCAAAAACGAGATCTTCATCAGTGCCACAAGAACAAGATGATGGTCCAGTACCCCTTAAAGAACAAAATGTCTCTGTATCAAAGAAAAGGAAAGCTCCATCTAAAAGAAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAR1 MULE SWIM

Protein Analysis

708

Amino Acids

81.52

Weight (kDa)

7.69

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 92 - 178 2.3e-21 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 268 - 381 1.9e-06 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
Transposase_mut PF00872 296 - 482 4.5e-09 Transposase, Mutator family
MULE PF10551 300 - 393 4.1e-29 MULE transposase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 282, 1455
Acc36I ACCTGC 1 cut(s) 1385
AccB7I CCANNNNNTGG 1 cut(s) 239
AccII CGCG 1 cut(s) 1532
AciI CCGC 3 cut(s) 721, 896, 1532
AclWI GGATC 3 cut(s) 1247, 1934, 2005
AcoI YGGCCR 1 cut(s) 1375
AcsI RAATTY 4 cut(s) 368, 485, 679, 1368
AcuI CTGAAG 1 cut(s) 443
AcvI CACGTG 1 cut(s) 166
AfaI GTAC 6 cut(s) 517, 1131, 1485, 1607, 1978, 2061
AfiI CCNNNNNNNGG 2 cut(s) 239, 584
AflII CTTAAG 1 cut(s) 553
AflIII ACRYGT 2 cut(s) 163, 547
AjnI CCWGG 1 cut(s) 1717
AluBI AGCT 5 cut(s) 509, 1058, 1100, 1232, 2105
AluI AGCT 5 cut(s) 509, 1058, 1100, 1232, 2105
Alw21I GWGCWC 2 cut(s) 949, 1217
Alw26I GTCTC 4 cut(s) 86, 1007, 1752, 2086
AlwI GGATC 3 cut(s) 1247, 1934, 2005
AoxI GGCC 1 cut(s) 1375
ApeKI GCWGC 3 cut(s) 1100, 1181, 1595
ApoI RAATTY 4 cut(s) 368, 485, 679, 1368
ArsI GACNNNNNNTTYG 2 cut(s) 1371, 1403
Asp700I GAANNNNTTC 2 cut(s) 1445, 1641
AspS9I GGNCC 2 cut(s) 665, 2054
AsuHPI GGTGA 6 cut(s) 101, 233, 755, 902, 1136, 1895
AsuII TTCGAA 2 cut(s) 814, 1645
AvaII GGWCC 2 cut(s) 665, 2054
BaeGI GKGCMC 2 cut(s) 150, 1366
BaeI ACNNNNGTAYC 2 cut(s) 129, 162
BbrPI CACGTG 1 cut(s) 166
Bbv12I GWGCWC 2 cut(s) 949, 1217
BbvI GCAGC 3 cut(s) 1087, 1168, 1607
BciT130I CCWGG 1 cut(s) 1719
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 4 cut(s) 86, 1007, 1752, 2086
BfaI CTAG 3 cut(s) 129, 294, 1113
BfrI CTTAAG 1 cut(s) 553
BfuAI ACCTGC 1 cut(s) 1385
BglII AGATCT 3 cut(s) 633, 1291, 2021
BisI GCNGC 3 cut(s) 1101, 1182, 1596
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 3 cut(s) 1102, 1183, 1597
BmcAI AGTACT 1 cut(s) 1131
Bme1390I CCNGG 1 cut(s) 1719
Bme18I GGWCC 2 cut(s) 665, 2054
BmgT120I GGNCC 2 cut(s) 665, 2054
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 1719
BmrI ACTGGG 2 cut(s) 144, 227
BmsI GCATC 5 cut(s) 67, 256, 736, 883, 1112
BmuI ACTGGG 2 cut(s) 144, 227
BpmI CTGGAG 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 2 cut(s) 814, 1645
BpuEI CTTGAG 5 cut(s) 105, 126, 567, 1213, 1753
Bsa29I ATCGAT 1 cut(s) 1106
BsaAI YACGTR 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 2026
BsaI GGTCTC 1 cut(s) 1007
BsaJI CCNNGG 2 cut(s) 1718, 1803
BsaWI WCCGGW 1 cut(s) 667
BsaXI ACNNNNNCTCC 2 cut(s) 779, 809
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 584
Bse1I ACTGG 4 cut(s) 150, 233, 1671, 2057
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 2026
BseBI CCWGG 1 cut(s) 1719
BseCI ATCGAT 1 cut(s) 1106
BseDI CCNNGG 2 cut(s) 1718, 1803
BseGI GGATG 5 cut(s) 71, 205, 449, 1796, 1849
BseJI GATNNNNATC 1 cut(s) 2026
BseLI CCNNNNNNNGG 2 cut(s) 239, 584
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 4 cut(s) 39, 314, 501, 1424
BseNI ACTGG 4 cut(s) 150, 233, 1671, 2057
BseSI GKGCMC 2 cut(s) 150, 1366
BseXI GCAGC 3 cut(s) 1087, 1168, 1607
BsgI GTGCAG 1 cut(s) 1413
Bsh1236I CGCG 1 cut(s) 1532
BshFI GGCC 1 cut(s) 1377
BshVI ATCGAT 1 cut(s) 1106
BsiHKAI GWGCWC 2 cut(s) 949, 1217
BsiSI CCGG 1 cut(s) 668
BslI CCNNNNNNNGG 2 cut(s) 239, 584
BsmAI GTCTC 4 cut(s) 86, 1007, 1752, 2086
BsnI GGCC 1 cut(s) 1377
Bso31I GGTCTC 1 cut(s) 1007
Bsp119I TTCGAA 2 cut(s) 814, 1645
Bsp1286I GDGCHC 4 cut(s) 150, 949, 1217, 1366
Bsp1407I TGTACA 1 cut(s) 1605
Bsp1720I GCTNAGC 1 cut(s) 510
BspACI CCGC 3 cut(s) 721, 896, 1532
BspANI GGCC 1 cut(s) 1377
BspCNI CTCAG 4 cut(s) 40, 315, 502, 1423
BspDI ATCGAT 1 cut(s) 1106
BspFNI CGCG 1 cut(s) 1532
BspLI GGNNCC 1 cut(s) 105
BspMI ACCTGC 1 cut(s) 1385
BspPI GGATC 3 cut(s) 1247, 1934, 2005
BspT104I TTCGAA 2 cut(s) 814, 1645
BspTI CTTAAG 1 cut(s) 553
BspTNI GGTCTC 1 cut(s) 1007
BsrDI GCAATG 1 cut(s) 459
BsrGI TGTACA 1 cut(s) 1605
BsrI ACTGG 4 cut(s) 150, 233, 1671, 2057
BssECI CCNNGG 2 cut(s) 1718, 1803
BssT1I CCWWGG 1 cut(s) 1803
Bst2UI CCWGG 1 cut(s) 1719
Bst4CI ACNGT 1 cut(s) 652
Bst6I CTCTTC 3 cut(s) 1263, 1507, 1632
BstAFI CTTAAG 1 cut(s) 553
BstAPI GCANNNNNTGC 2 cut(s) 1109, 1778
BstAUI TGTACA 1 cut(s) 1605
BstBAI YACGTR 1 cut(s) 166
BstBI TTCGAA 2 cut(s) 814, 1645
BstC8I GCNNGC 6 cut(s) 213, 314, 723, 1027, 1497, 1530
BstDEI CTNAG 4 cut(s) 48, 323, 510, 1410
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 5 cut(s) 71, 205, 449, 1796, 1849
BstFNI CGCG 1 cut(s) 1532
BstMAI GTCTC 4 cut(s) 86, 1007, 1752, 2086
BstMWI GCNNNNNNNGC 5 cut(s) 1109, 1538, 1555, 1778, 1963
BstNI CCWGG 1 cut(s) 1719
BstNSI RCATGY 2 cut(s) 551, 1029
BstSCI CCNGG 1 cut(s) 1717
BstSLI GKGCMC 2 cut(s) 150, 1366
BstUI CGCG 1 cut(s) 1532
BstV1I GCAGC 3 cut(s) 1087, 1168, 1607
BstX2I RGATCY 4 cut(s) 633, 1291, 1997, 2021
BstYI RGATCY 4 cut(s) 633, 1291, 1997, 2021
Bsu15I ATCGAT 1 cut(s) 1106
BsuRI GGCC 1 cut(s) 1377
BsuTUI ATCGAT 1 cut(s) 1106
BtsCI GGATG 5 cut(s) 71, 205, 449, 1796, 1849
BtsIMutI CAGTG 3 cut(s) 45, 157, 2037
BveI ACCTGC 1 cut(s) 1385
Cac8I GCNNGC 6 cut(s) 213, 314, 723, 1027, 1497, 1530
Cfr13I GGNCC 2 cut(s) 665, 2054
ClaI ATCGAT 1 cut(s) 1106
CseI GACGC 1 cut(s) 1389
Csp6I GTAC 6 cut(s) 516, 1130, 1484, 1606, 1977, 2060
CviAII CATG 7 cut(s) 357, 521, 548, 937, 971, 1026, 1871
CviQI GTAC 6 cut(s) 516, 1130, 1484, 1606, 1977, 2060
DdeI CTNAG 4 cut(s) 48, 323, 510, 1410
DraI TTTAAA 1 cut(s) 1090
EaeI YGGCCR 1 cut(s) 1375
Eam1104I CTCTTC 3 cut(s) 1263, 1507, 1632
EarI CTCTTC 3 cut(s) 1263, 1507, 1632
Eco130I CCWWGG 1 cut(s) 1803
Eco31I GGTCTC 1 cut(s) 1007
Eco47I GGWCC 2 cut(s) 665, 2054
Eco57I CTGAAG 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 1717
EcoT14I CCWWGG 1 cut(s) 1803
EcoT22I ATGCAT 1 cut(s) 271
ErhI CCWWGG 1 cut(s) 1803
FaeI CATG 7 cut(s) 360, 524, 551, 940, 974, 1029, 1874
FalI AAGNNNNNCTT 2 cut(s) 1281, 1313
FatI CATG 7 cut(s) 356, 520, 547, 936, 970, 1025, 1870
FauI CCCGC 1 cut(s) 714
FauNDI CATATG 3 cut(s) 271, 289, 1702
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 3 cut(s) 1101, 1182, 1596
FokI GGATG 5 cut(s) 78, 212, 456, 1783, 1856
Fsp4HI GCNGC 3 cut(s) 1101, 1182, 1596
FspBI CTAG 3 cut(s) 129, 294, 1113
GluI GCNGC 3 cut(s) 1101, 1182, 1596
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 1 cut(s) 1377
HapII CCGG 1 cut(s) 668
HgaI GACGC 1 cut(s) 1389
Hin1II CATG 7 cut(s) 360, 524, 551, 940, 974, 1029, 1874
HindIII AAGCTT 1 cut(s) 1230
HpaII CCGG 1 cut(s) 668
HphI GGTGA 6 cut(s) 101, 233, 755, 902, 1136, 1895
Hpy166II GTNNAC 3 cut(s) 545, 1486, 1608
Hpy188I TCNGA 4 cut(s) 443, 462, 1413, 1897
Hpy188III TCNNGA 6 cut(s) 122, 263, 413, 533, 1256, 1634
Hpy8I GTNNAC 3 cut(s) 545, 1486, 1608
Hpy99I CGWCG 2 cut(s) 656, 1383
HpyAV CCTTC 8 cut(s) 176, 312, 380, 588, 695, 711, 925, 1956
HpyCH4III ACNGT 1 cut(s) 652
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 5 cut(s) 1109, 1538, 1555, 1778, 1963
HpyF3I CTNAG 4 cut(s) 48, 323, 510, 1410
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 7 cut(s) 360, 524, 551, 940, 974, 1029, 1874
Ksp22I TGATCA 1 cut(s) 259
LmnI GCTCC 2 cut(s) 1220, 2110
Lsp1109I GCAGC 3 cut(s) 1087, 1168, 1607
LweI GCATC 5 cut(s) 67, 256, 736, 883, 1112
MaeI CTAG 3 cut(s) 129, 294, 1113
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 5 cut(s) 41, 352, 493, 558, 1739
MfeI CAATTG 2 cut(s) 81, 1624
MflI RGATCY 4 cut(s) 633, 1291, 1997, 2021
MhlI GDGCHC 4 cut(s) 150, 949, 1217, 1366
MlyI GAGTC 2 cut(s) 1204, 1820
MmeI TCCRAC 4 cut(s) 937, 1265, 1803, 1809
Mph1103I ATGCAT 1 cut(s) 271
MroXI GAANNNNTTC 2 cut(s) 1445, 1641
MseI TTAA 6 cut(s) 554, 771, 963, 1089, 1950, 2067
MslI CAYNNNNRTG 2 cut(s) 348, 1030
MspCI CTTAAG 1 cut(s) 553
MspI CCGG 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 1719
MunI CAATTG 2 cut(s) 81, 1624
MvaI CCWGG 1 cut(s) 1719
MvnI CGCG 1 cut(s) 1532
MwoI GCNNNNNNNGC 5 cut(s) 1109, 1538, 1555, 1778, 1963
NdeI CATATG 3 cut(s) 271, 289, 1702
NlaIII CATG 7 cut(s) 360, 524, 551, 940, 974, 1029, 1874
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 4 cut(s) 352, 493, 558, 1739
NsiI ATGCAT 1 cut(s) 271
NspI RCATGY 2 cut(s) 551, 1029
NspV TTCGAA 2 cut(s) 814, 1645
PaeI GCATGC 1 cut(s) 1029
PciI ACATGT 1 cut(s) 547
PdmI GAANNNNTTC 2 cut(s) 1445, 1641
PfeI GAWTC 9 cut(s) 20, 170, 438, 536, 598, 1277, 1697, 1723, 1892
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 3 cut(s) 1102, 1183, 1597
PleI GAGTC 2 cut(s) 1203, 1819
PmaCI CACGTG 1 cut(s) 166
PmlI CACGTG 1 cut(s) 166
PpsI GAGTC 2 cut(s) 1203, 1819
Ppu21I YACGTR 1 cut(s) 166
PscI ACATGT 1 cut(s) 547
PsiI TTATAA 2 cut(s) 282, 1455
Psp6I CCWGG 1 cut(s) 1717
PspCI CACGTG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 1717
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 2 cut(s) 665, 2054
PsuI RGATCY 4 cut(s) 633, 1291, 1997, 2021
RsaI GTAC 6 cut(s) 517, 1131, 1485, 1607, 1978, 2061
RsaNI GTAC 6 cut(s) 516, 1130, 1484, 1606, 1977, 2060
RseI CAYNNNNRTG 2 cut(s) 348, 1030
SaqAI TTAA 6 cut(s) 554, 771, 963, 1089, 1950, 2067
SatI GCNGC 3 cut(s) 1101, 1182, 1596
Sau96I GGNCC 2 cut(s) 665, 2054
ScaI AGTACT 1 cut(s) 1131
SchI GAGTC 2 cut(s) 1204, 1820
ScrFI CCNGG 1 cut(s) 1719
SduI GDGCHC 4 cut(s) 150, 949, 1217, 1366
SfaNI GCATC 5 cut(s) 67, 256, 736, 883, 1112
SfuI TTCGAA 2 cut(s) 814, 1645
SinI GGWCC 2 cut(s) 665, 2054
SmiMI CAYNNNNRTG 2 cut(s) 348, 1030
SmlI CTYRAG 6 cut(s) 120, 141, 553, 582, 1192, 1732
SmoI CTYRAG 6 cut(s) 120, 141, 553, 582, 1192, 1732
SphI GCATGC 1 cut(s) 1029
SsiI CCGC 3 cut(s) 721, 896, 1532
SspI AATATT 2 cut(s) 1086, 1987
SspMI CTAG 3 cut(s) 129, 294, 1113
StyD4I CCNGG 1 cut(s) 1717
StyI CCWWGG 1 cut(s) 1803
TaaI ACNGT 1 cut(s) 652
TaiI ACGT 1 cut(s) 168
TaqI TCGA 7 cut(s) 654, 814, 825, 949, 1106, 1510, 1645
TatI WGTACW 2 cut(s) 1129, 1605
TfiI GAWTC 9 cut(s) 20, 170, 438, 536, 598, 1277, 1697, 1723, 1892
Tru1I TTAA 6 cut(s) 554, 771, 963, 1089, 1950, 2067
Tru9I TTAA 6 cut(s) 554, 771, 963, 1089, 1950, 2067
TscAI CASTG 3 cut(s) 52, 157, 2037
TseFI GTSAC 4 cut(s) 352, 493, 558, 1739
TseI GCWGC 3 cut(s) 1100, 1181, 1595
Tsp45I GTSAC 4 cut(s) 352, 493, 558, 1739
TspRI CASTG 3 cut(s) 52, 157, 2037
Van91I CCANNNNNTGG 1 cut(s) 239
Vha464I CTTAAG 1 cut(s) 553
VpaK11BI GGWCC 2 cut(s) 665, 2054
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 4 cut(s) 368, 485, 679, 1368
XceI RCATGY 2 cut(s) 551, 1029
XmnI GAANNNNTTC 2 cut(s) 1445, 1641
XspI CTAG 3 cut(s) 129, 294, 1113
ZrmI AGTACT 1 cut(s) 1131
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.