Rorug07G0023300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0002145
Physical Location & Seq
Forward (+)
1670317 .. 1680953
10637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0023300.1

Sequence Viewer

Length: 291 bp
ATGAAGGCATCATATGTCGCAATTGCATTATGCACTGTGCTTGTGGTGATGCTGGCCACAGCAGAGGTGTCCATGGCAGCTGTGACTTGCAGCCCAGTAGAGCTGAGCTCCTGTGCAAGTGCAATAAGCTCTAATACTCCACCATCAAATCTATGCTGCACCAAAATCAAGGAGCAGAAGCCTTGTCTCTGCCAGTACCTCAACAACCCCAACCTCAAGAAATTCGTTAACACCCCGAATGCTCGGAGAGTTGCTAGGACTTGTGGCACTCCCTTCCCCAGGTGCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

96

Amino Acids

10.23

Weight (kDa)

9.11

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 34 - 95 6.3e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 221
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 279
AjnI CCWGG 1 cut(s) 278
AluBI AGCT 5 cut(s) 80, 103, 108, 129, 288
AluI AGCT 5 cut(s) 80, 103, 108, 129, 288
Alw21I GWGCWC 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 191
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 4 cut(s) 77, 90, 156, 285
ApoI RAATTY 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 58
BalI TGGCCA 1 cut(s) 56
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 110
BbvI GCAGC 3 cut(s) 89, 102, 143
BccI CCATC 1 cut(s) 151
BciT130I CCWGG 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 2 cut(s) 255, 289
BisI GCNGC 4 cut(s) 78, 91, 157, 286
BlpI GCTNAGC 1 cut(s) 104
BlsI GCNGC 4 cut(s) 79, 92, 158, 287
Bme1390I CCNGG 1 cut(s) 280
BmrFI CCNGG 1 cut(s) 280
BmrI ACTGGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 17, 39
BmuI ACTGGG 1 cut(s) 89
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu1102I GCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 72, 278
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 2 cut(s) 95, 193
BseBI CCWGG 1 cut(s) 280
BseDI CCNNGG 2 cut(s) 72, 278
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 95, 193
BseXI GCAGC 3 cut(s) 89, 102, 143
BsgI GTGCAG 1 cut(s) 142
BshFI GGCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 110
Bsp1720I GCTNAGC 1 cut(s) 104
Bsp19I CCATGG 1 cut(s) 72
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 96
BsrI ACTGG 2 cut(s) 95, 193
BssECI CCNNGG 2 cut(s) 72, 278
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 104
BstDSI CCRYGG 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 191
BstNI CCWGG 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 278
BstV1I GCAGC 3 cut(s) 89, 102, 143
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 54
Csp6I GTAC 1 cut(s) 196
CviAII CATG 1 cut(s) 73
CviJI RGCY 8 cut(s) 56, 80, 93, 103, 108, 129, 181, 288
CviKI_1 RGCY 8 cut(s) 56, 80, 93, 103, 108, 129, 181, 288
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 1 cut(s) 104
EaeI YGGCCR 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 110
Eco53kI GAGCTC 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 277
EcoRII CCWGG 1 cut(s) 278
EcoT14I CCWWGG 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 110
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 1 cut(s) 76
FaiI YATR 5 cut(s) 13, 15, 31, 74, 154
FatI CATG 1 cut(s) 72
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 4 cut(s) 78, 91, 157, 286
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 4 cut(s) 78, 91, 157, 286
FspBI CTAG 2 cut(s) 255, 289
GluI GCNGC 4 cut(s) 78, 91, 157, 286
HaeIII GGCC 1 cut(s) 56
Hin1II CATG 1 cut(s) 76
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 1 cut(s) 246
Hpy188III TCNNGA 1 cut(s) 217
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 1 cut(s) 283
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 7 cut(s) 26, 33, 90, 116, 122, 159, 285
HpyF3I CTNAG 1 cut(s) 104
Hsp92II CATG 1 cut(s) 76
KspAI GTTAAC 1 cut(s) 229
LmnI GCTCC 2 cut(s) 113, 172
LpnPI CCDG 5 cut(s) 38, 108, 124, 206, 265
Lsp1109I GCAGC 3 cut(s) 89, 102, 143
LweI GCATC 2 cut(s) 17, 39
MaeI CTAG 2 cut(s) 255, 289
MaeIII GTNAC 1 cut(s) 82
MfeI CAATTG 1 cut(s) 21
MhlI GDGCHC 1 cut(s) 110
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 2 cut(s) 21, 221
MluNI TGGCCA 1 cut(s) 56
MnlI CCTC 3 cut(s) 58, 209, 224
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 56
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 280
MunI CAATTG 1 cut(s) 21
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 1 cut(s) 280
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 1 cut(s) 13
NlaIII CATG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 82
PctI GAATGC 1 cut(s) 244
PkrI GCNGC 4 cut(s) 79, 92, 158, 287
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 278
PspGI CCWGG 1 cut(s) 278
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
SacI GAGCTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 4 cut(s) 78, 91, 157, 286
ScrFI CCNGG 1 cut(s) 280
SduI GDGCHC 1 cut(s) 110
SetI ASST 9 cut(s) 69, 82, 105, 110, 131, 201, 216, 284, 290
SfaNI GCATC 2 cut(s) 17, 39
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 2 cut(s) 21, 221
SspMI CTAG 2 cut(s) 255, 289
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 278
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 1 cut(s) 37
TasI AATT 2 cut(s) 21, 221
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 4 cut(s) 77, 90, 156, 285
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 40
XagI CCTNNNNNAGG 1 cut(s) 277
XapI RAATTY 1 cut(s) 221
XspI CTAG 2 cut(s) 255, 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.