Rroxscaffold_1G00031400

ribosome biogenesis GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005565
Physical Location & Seq
Reverse (-)
44132937 .. 44142720
9784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031400.1

Sequence Viewer

Length: 1020 bp
ATGCCGTGCCTACTCACCGCCCACCGCCGACACCTCGATCTCCACCACCGCTCCGTCATCGCCCACCGTCACCATCACTCTCCCCACCTTCAACGACCCTCTCGCCGCCGATTCTCAACCGTCCAACGCGTCCCTATCGCAGCCGCAAAGCACCCTAACGTCACCGAAAATCTCGGCTACCGAACAAGAAGCTCCTCCGAGCTAAACGGACCTTCAAGGACTACTCCTCCCTCGCCCGGTCCTCTCTCCTCGGGACAAGCCTCCTCTCTCGGAATCCCGGACCGTCGGCACCGTCGCCGCCGCTCGTGCCAACTTCATGCGCGTCATCGTCGAACCTCCCGGAAGCCTCGAGAAGAGCGCGGACGAAATGGCGTCGTCGTCTCTCGGATTTCGGTGGAGCCGCTCGTGGGTGGTGAGGGCGTTGTTGAAGAAGATAAAGAGGAGAGTGTTGGTTGGGGACAAGTGGGGTCCATTGACCGGGTGGACCGCCGGGGAATGATCGAGAATGTGTACCGGAGGAGCTCCGAGATTCTTGACCCCGCCGGTGGCCAATGTGGACCATCCGCTTGTGCTTTTCTCTCGGAGCAGCCCAAGCTTGACCCCTTCTCTCTCACTAGTTTTCTAATTGAGGCCGAGTCCACCGGAATTCCACTCACTCCGGCGCTCAACAAGTCCGAGCTGGTTGACGAAGAGACGAAGGCTACATGGAAGTCTAGGCCACATAACAAGCCTCGCTATGAAGATCAAGTCAAGGACCTGGTCAGCTCCGGTCAACTCGACTCACCGCCGCCGCCGCAGGCCGCGCCGCCGTGCAAGGCCACCACGGACAGTGCGCAAGGACCATACGAAGCTAATGGAAGAAGTTTGATGAAGATTGGTGGTCGGAGGAGGAAGATATGGCCGGATCAAGAACTCAAGTGGCTTGAAGACTGGTTAAGTGGTACTTGCAGAGAGTTTACATCAGAGATTTTAGGTAGGGGGTCTTTAGTGGTTAGACTACCTTGGGTTTCGATCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00730 ko01100 map00730 map01100
Pfam Domains
Protein Families
RsgA_GTPase

Protein Analysis

339

Amino Acids

37.85

Weight (kDa)

9.97

Isoelectric Point (pI)

73.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 834
AccB1I GGYRCC 1 cut(s) 288
AccBSI CCGCTC 3 cut(s) 51, 303, 403
AccII CGCG 4 cut(s) 129, 322, 360, 803
AclWI GGATC 1 cut(s) 912
AcoI YGGCCR 2 cut(s) 547, 899
AcsI RAATTY 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 372
AfaI GTAC 2 cut(s) 512, 943
AfiI CCNNNNNNNGG 4 cut(s) 236, 407, 477, 545
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 4 cut(s) 92, 216, 428, 926
AhlI ACTAGT 1 cut(s) 614
AjnI CCWGG 1 cut(s) 756
AluBI AGCT 7 cut(s) 192, 202, 522, 595, 679, 765, 851
AluI AGCT 7 cut(s) 192, 202, 522, 595, 679, 765, 851
Alw21I GWGCWC 1 cut(s) 524
Alw26I GTCTC 2 cut(s) 385, 686
AlwI GGATC 1 cut(s) 912
Ama87I CYCGRG 2 cut(s) 250, 348
AoxI GGCC 6 cut(s) 547, 630, 716, 798, 816, 899
ApeKI GCWGC 2 cut(s) 140, 586
ApoI RAATTY 1 cut(s) 645
AspLEI GCGC 5 cut(s) 322, 360, 664, 805, 835
AspS9I GGNCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
AsuC2I CCSGG 5 cut(s) 237, 278, 340, 479, 491
AsuHPI GGTGA 5 cut(s) 7, 62, 154, 425, 774
AvaI CYCGRG 2 cut(s) 250, 348
AvaII GGWCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
BalI TGGCCA 1 cut(s) 549
BanI GGYRCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 524
BauI CACGAG 2 cut(s) 304, 404
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 524
BbvI GCAGC 2 cut(s) 152, 598
BccI CCATC 2 cut(s) 81, 568
BceAI ACGGC 1 cut(s) 793
BciT130I CCWGG 1 cut(s) 758
BcnI CCSGG 5 cut(s) 237, 278, 340, 479, 491
BcoDI GTCTC 2 cut(s) 385, 686
BcuI ACTAGT 1 cut(s) 614
BfaI CTAG 2 cut(s) 615, 714
BfoI RGCGCY 1 cut(s) 665
Bme1390I CCNGG 6 cut(s) 237, 278, 340, 479, 491, 758
Bme18I GGWCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
BmeT110I CYCGRG 2 cut(s) 250, 348
BmgT120I GGNCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
BmiI GGNNCC 3 cut(s) 290, 399, 469
BmrFI CCNGG 6 cut(s) 237, 278, 340, 479, 491, 758
BpiI GAAGAC 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 5 cut(s) 237, 278, 340, 479, 491
BsaHI GRCGYC 1 cut(s) 372
BsaJI CCNNGG 4 cut(s) 249, 490, 822, 1001
BsaWI WCCGGW 3 cut(s) 513, 641, 767
BsaXI ACNNNNNCTCC 4 cut(s) 35, 65, 211, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 236, 407, 477, 545
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 935
BseBI CCWGG 1 cut(s) 758
BseDI CCNNGG 4 cut(s) 249, 490, 822, 1001
BseGI GGATG 1 cut(s) 560
BseLI CCNNNNNNNGG 4 cut(s) 236, 407, 477, 545
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 7 cut(s) 184, 216, 238, 253, 455, 532, 901
BseXI GCAGC 2 cut(s) 152, 598
Bsh1236I CGCG 4 cut(s) 129, 322, 360, 803
BshFI GGCC 6 cut(s) 549, 632, 718, 800, 818, 901
BshNI GGYRCC 1 cut(s) 288
BsiHKAI GWGCWC 1 cut(s) 524
BsiHKCI CYCGRG 2 cut(s) 250, 348
BslFI GGGAC 3 cut(s) 116, 267, 471
BslI CCNNNNNNNGG 4 cut(s) 236, 407, 477, 545
BsmAI GTCTC 2 cut(s) 385, 686
BsmBI CGTCTC 2 cut(s) 385, 686
BsmFI GGGAC 3 cut(s) 116, 267, 471
BsnI GGCC 6 cut(s) 549, 632, 718, 800, 818, 901
BsoBI CYCGRG 2 cut(s) 250, 348
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 5 cut(s) 37, 498, 742, 904, 1011
BspANI GGCC 6 cut(s) 549, 632, 718, 800, 818, 901
BspFNI CGCG 4 cut(s) 129, 322, 360, 803
BspLI GGNNCC 3 cut(s) 290, 399, 469
BspPI GGATC 1 cut(s) 912
BspQI GCTCTTC 1 cut(s) 348
BspT107I GGYRCC 1 cut(s) 288
BsrBI CCGCTC 3 cut(s) 51, 303, 403
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 1 cut(s) 935
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 4 cut(s) 249, 490, 822, 1001
BssMI GATC 5 cut(s) 37, 498, 742, 904, 1011
BssNI GRCGYC 1 cut(s) 372
BssSI CACGAG 2 cut(s) 304, 404
BssT1I CCWWGG 1 cut(s) 1001
Bst2BI CACGAG 2 cut(s) 304, 404
Bst2UI CCWGG 1 cut(s) 758
Bst4CI ACNGT 5 cut(s) 68, 121, 284, 293, 830
Bst6I CTCTTC 2 cut(s) 348, 684
BstACI GRCGYC 1 cut(s) 372
BstC8I GCNNGC 1 cut(s) 798
BstDSI CCRYGG 1 cut(s) 822
BstF5I GGATG 1 cut(s) 560
BstFNI CGCG 4 cut(s) 129, 322, 360, 803
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 5 cut(s) 322, 360, 664, 805, 835
BstKTI GATC 5 cut(s) 40, 501, 745, 907, 1014
BstMAI GTCTC 2 cut(s) 385, 686
BstMBI GATC 5 cut(s) 37, 498, 742, 904, 1011
BstMWI GCNNNNNNNGC 4 cut(s) 306, 592, 793, 802
BstNI CCWGG 1 cut(s) 758
BstSCI CCNGG 6 cut(s) 235, 276, 338, 477, 489, 756
BstUI CGCG 4 cut(s) 129, 322, 360, 803
BstV1I GCAGC 2 cut(s) 152, 598
BstV2I GAAGAC 1 cut(s) 933
BsuRI GGCC 6 cut(s) 549, 632, 718, 800, 818, 901
BtgI CCRYGG 1 cut(s) 822
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 1 cut(s) 560
BtsIMutI CAGTG 1 cut(s) 835
Cac8I GCNNGC 1 cut(s) 798
CfoI GCGC 5 cut(s) 322, 360, 664, 805, 835
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
CpoI CGGWCCG 1 cut(s) 280
CseI GACGC 3 cut(s) 118, 311, 361
CsiI ACCWGGT 1 cut(s) 756
Csp6I GTAC 2 cut(s) 511, 942
CspI CGGWCCG 1 cut(s) 280
CviAII CATG 2 cut(s) 317, 705
CviQI GTAC 2 cut(s) 511, 942
DpnI GATC 5 cut(s) 39, 500, 744, 906, 1013
DpnII GATC 5 cut(s) 37, 498, 742, 904, 1011
EaeI YGGCCR 2 cut(s) 547, 899
Eam1104I CTCTTC 2 cut(s) 348, 684
EarI CTCTTC 2 cut(s) 348, 684
Ecl136II GAGCTC 1 cut(s) 522
Eco130I CCWWGG 1 cut(s) 1001
Eco24I GRGCYC 1 cut(s) 524
Eco47I GGWCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
Eco53kI GAGCTC 1 cut(s) 522
Eco88I CYCGRG 2 cut(s) 250, 348
EcoICRI GAGCTC 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 754
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 1 cut(s) 756
EcoT14I CCWWGG 1 cut(s) 1001
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 1001
Esp3I CGTCTC 2 cut(s) 385, 686
FaeI CATG 2 cut(s) 320, 708
FaiI YATR 6 cut(s) 318, 706, 723, 738, 844, 898
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 3 cut(s) 116, 267, 471
FatI CATG 2 cut(s) 316, 704
FauI CCCGC 1 cut(s) 547
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 1 cut(s) 524
FspBI CTAG 2 cut(s) 615, 714
FspI TGCGCA 1 cut(s) 834
GlaI GCGC 5 cut(s) 321, 359, 663, 804, 834
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 6 cut(s) 549, 632, 718, 800, 818, 901
HgaI GACGC 3 cut(s) 118, 311, 361
HhaI GCGC 5 cut(s) 322, 360, 664, 805, 835
Hin1I GRCGYC 1 cut(s) 372
Hin1II CATG 2 cut(s) 320, 708
Hin6I GCGC 5 cut(s) 320, 358, 662, 803, 833
HinP1I GCGC 5 cut(s) 320, 358, 662, 803, 833
HincII GTYRAC 2 cut(s) 685, 773
HindII GTYRAC 2 cut(s) 685, 773
HindIII AAGCTT 1 cut(s) 593
HinfI GANTC 5 cut(s) 111, 273, 529, 635, 779
HphI GGTGA 5 cut(s) 7, 62, 154, 425, 774
Hpy166II GTNNAC 7 cut(s) 484, 511, 557, 639, 685, 773, 957
Hpy188I TCNGA 8 cut(s) 199, 272, 387, 526, 583, 676, 885, 964
Hpy188III TCNNGA 5 cut(s) 252, 350, 502, 533, 908
Hpy8I GTNNAC 7 cut(s) 484, 511, 557, 639, 685, 773, 957
Hpy99I CGWCG 5 cut(s) 288, 297, 333, 377, 380
HpyAV CCTTC 4 cut(s) 98, 222, 613, 691
HpyCH4III ACNGT 5 cut(s) 68, 121, 284, 293, 830
HpyCH4IV ACGT 1 cut(s) 159
HpyCH4V TGCA 2 cut(s) 813, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 306, 592, 793, 802
HpySE526I ACGT 1 cut(s) 159
Hsp92I GRCGYC 1 cut(s) 372
Hsp92II CATG 2 cut(s) 320, 708
HspAI GCGC 5 cut(s) 320, 358, 662, 803, 833
Kzo9I GATC 5 cut(s) 37, 498, 742, 904, 1011
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 7 cut(s) 56, 197, 397, 519, 527, 583, 770
Lsp1109I GCAGC 2 cut(s) 152, 598
MabI ACCWGGT 1 cut(s) 756
MaeI CTAG 2 cut(s) 615, 714
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 68, 160
MalI GATC 5 cut(s) 39, 500, 744, 906, 1013
MbiI CCGCTC 3 cut(s) 51, 303, 403
MboI GATC 5 cut(s) 37, 498, 742, 904, 1011
MboII GAAGA 9 cut(s) 365, 440, 443, 701, 752, 870, 883, 904, 938
MhlI GDGCHC 1 cut(s) 524
MlsI TGGCCA 1 cut(s) 549
MluCI AATT 2 cut(s) 624, 645
MluI ACGCGT 1 cut(s) 127
MluNI TGGCCA 1 cut(s) 549
MlyI GAGTC 2 cut(s) 644, 773
MmeI TCCRAC 2 cut(s) 148, 863
Mox20I TGGCCA 1 cut(s) 549
MscI TGGCCA 1 cut(s) 549
MseI TTAA 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 549
MspR9I CCNGG 6 cut(s) 237, 278, 340, 479, 491, 758
MvaI CCWGG 1 cut(s) 758
MvnI CGCG 4 cut(s) 129, 322, 360, 803
MwoI GCNNNNNNNGC 4 cut(s) 306, 592, 793, 802
NciI CCSGG 5 cut(s) 237, 278, 340, 479, 491
NdeII GATC 5 cut(s) 37, 498, 742, 904, 1011
NlaIII CATG 2 cut(s) 320, 708
NlaIV GGNNCC 3 cut(s) 290, 399, 469
NmeAIII GCCGAG 2 cut(s) 153, 658
NmuCI GTSAC 2 cut(s) 68, 160
NsbI TGCGCA 1 cut(s) 834
PaeR7I CTCGAG 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 348
PfeI GAWTC 3 cut(s) 111, 273, 529
PflFI GACNNNGTC 1 cut(s) 758
PfoI TCCNGGA 2 cut(s) 276, 338
PleI GAGTC 2 cut(s) 643, 773
PpsI GAGTC 2 cut(s) 643, 773
PpuMI RGGWCCY 1 cut(s) 754
Psp124BI GAGCTC 1 cut(s) 524
Psp5II RGGWCCY 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 756
PspGI CCWGG 1 cut(s) 756
PspN4I GGNNCC 3 cut(s) 290, 399, 469
PspPI GGNCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
PspPPI RGGWCCY 1 cut(s) 754
PsyI GACNNNGTC 1 cut(s) 758
RsaI GTAC 2 cut(s) 512, 943
RsaNI GTAC 2 cut(s) 511, 942
Rsr2I CGGWCCG 1 cut(s) 280
RsrII CGGWCCG 1 cut(s) 280
SacI GAGCTC 1 cut(s) 524
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 935
Sau3AI GATC 5 cut(s) 37, 498, 742, 904, 1011
Sau96I GGNCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
SchI GAGTC 2 cut(s) 644, 773
ScrFI CCNGG 6 cut(s) 237, 278, 340, 479, 491, 758
SduI GDGCHC 1 cut(s) 524
SexAI ACCWGGT 1 cut(s) 756
Sfr274I CTCGAG 1 cut(s) 348
SgrAI CRCCGGYG 1 cut(s) 542
SinI GGWCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
SlaI CTCGAG 1 cut(s) 348
SmlI CTYRAG 2 cut(s) 348, 914
SmoI CTYRAG 2 cut(s) 348, 914
SpeI ACTAGT 1 cut(s) 614
Sse9I AATT 2 cut(s) 624, 645
SspMI CTAG 2 cut(s) 615, 714
SstI GAGCTC 1 cut(s) 524
StyD4I CCNGG 6 cut(s) 235, 276, 338, 477, 489, 756
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 5 cut(s) 68, 121, 284, 293, 830
TaiI ACGT 1 cut(s) 162
TaqI TCGA 6 cut(s) 36, 331, 349, 501, 777, 1010
TasI AATT 2 cut(s) 624, 645
TfiI GAWTC 3 cut(s) 111, 273, 529
Tru1I TTAA 1 cut(s) 935
Tru9I TTAA 1 cut(s) 935
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 2 cut(s) 68, 160
TseI GCWGC 2 cut(s) 140, 586
Tsp45I GTSAC 2 cut(s) 68, 160
TspDTI ATGAA 3 cut(s) 305, 753, 884
TspGWI ACGGA 3 cut(s) 43, 222, 839
TspRI CASTG 1 cut(s) 835
Tth111I GACNNNGTC 1 cut(s) 758
VpaK11BI GGWCC 8 cut(s) 209, 239, 280, 468, 484, 557, 754, 839
XapI RAATTY 1 cut(s) 645
XcmI CCANNNNNNNNNTGG 1 cut(s) 478
XhoI CTCGAG 1 cut(s) 348
XspI CTAG 2 cut(s) 615, 714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.