Rroxscaffold_5G00353660

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005565
Physical Location & Seq
Forward (+)
31548147 .. 31548554
408 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00353660.1

Sequence Viewer

Length: 408 bp
ATGGTTGTGGCAGTTCAAGTCGAAATGGGAGCTGGAGGAAGCAATGATCATGCCGAAATCAGTACACCAACCATTGATCTTCAGGGCAAAGCGCAAGGAGACCCGGCCTTACGAGCCGAGCTCATCCGAAAAGTTCGATATGCATGTGAGAAATGGGGCTTCTTTCGGGTAGTGAACCATGGAATTCCGTTGTTGATGTCTTGGATAGGATTAATTACAAATGGAATACGTGAGTTCAATGAGCAGGATAGTGAGCTTAAGAAGAAGTTTTATAAGCGGTTTGGGACGAAGGTGTTTTATCGGTCCAACTACAATTCGTACCAATCTTCATCACCGATTGGAGGGCTACATTGGGCTGTTTTGTGGCTCTCGATCCACCCAAACCAGAAGAAGTACCCTCGTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

135

Amino Acids

15.4

Weight (kDa)

9.68

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 23 - 100 1.6e-12 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AciI CCGC 1 cut(s) 277
AclWI GGATC 1 cut(s) 367
AcsI RAATTY 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 3 cut(s) 64, 320, 395
AfiI CCNNNNNNNGG 1 cut(s) 341
AflII CTTAAG 1 cut(s) 257
AgsI TTSAA 2 cut(s) 17, 238
AluBI AGCT 3 cut(s) 32, 121, 256
AluI AGCT 3 cut(s) 32, 121, 256
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 1 cut(s) 367
AoxI GGCC 1 cut(s) 105
ApoI RAATTY 1 cut(s) 183
AseI ATTAAT 1 cut(s) 212
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 303
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 1 cut(s) 324
AvaII GGWCC 1 cut(s) 303
BanII GRGCYC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 123
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 104
Bme18I GGWCC 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 303
BmrFI CCNGG 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 54
BpuMI CCSGG 1 cut(s) 104
BsaAI YACGTR 1 cut(s) 230
BsaI GGTCTC 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 341
Bse3DI GCAATG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 341
BseMI GCAATG 1 cut(s) 49
BshFI GGCC 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 1 cut(s) 107
Bso31I GGTCTC 1 cut(s) 93
Bsp1286I GDGCHC 1 cut(s) 123
Bsp143I GATC 3 cut(s) 46, 76, 372
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 1 cut(s) 277
BspANI GGCC 1 cut(s) 107
BspPI GGATC 1 cut(s) 367
BspTI CTTAAG 1 cut(s) 257
BspTNI GGTCTC 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 3 cut(s) 46, 76, 372
BssT1I CCWWGG 1 cut(s) 178
BstAFI CTTAAG 1 cut(s) 257
BstBAI YACGTR 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 3 cut(s) 49, 79, 375
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 3 cut(s) 46, 76, 372
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 102
BsuRI GGCC 1 cut(s) 107
BtgI CCRYGG 1 cut(s) 178
BtsCI GGATG 1 cut(s) 123
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 303
Csp6I GTAC 3 cut(s) 63, 319, 394
CviAII CATG 3 cut(s) 50, 144, 179
CviJI RGCY 9 cut(s) 32, 107, 116, 121, 159, 256, 346, 356, 367
CviKI_1 RGCY 9 cut(s) 32, 107, 116, 121, 159, 256, 346, 356, 367
CviQI GTAC 3 cut(s) 63, 319, 394
DpnI GATC 3 cut(s) 48, 78, 374
DpnII GATC 3 cut(s) 46, 76, 372
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 3 cut(s) 53, 147, 182
FaiI YATR 5 cut(s) 51, 141, 145, 180, 273
FaqI GGGAC 1 cut(s) 298
FatI CATG 3 cut(s) 49, 143, 178
FbaI TGATCA 1 cut(s) 46
FokI GGATG 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 123
GlaI GCGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 54
HaeIII GGCC 1 cut(s) 107
HapII CCGG 1 cut(s) 104
HhaI GCGC 1 cut(s) 94
Hin1II CATG 3 cut(s) 53, 147, 182
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 324
Hpy166II GTNNAC 2 cut(s) 65, 175
Hpy188I TCNGA 1 cut(s) 128
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 2 cut(s) 65, 175
HpyAV CCTTC 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 1 cut(s) 143
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 3 cut(s) 53, 147, 182
HspAI GCGC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 46, 76, 372
LmnI GCTCC 1 cut(s) 29
LpnPI CCDG 5 cut(s) 18, 68, 117, 230, 398
MaeII ACGT 1 cut(s) 229
MalI GATC 3 cut(s) 48, 78, 374
MboI GATC 3 cut(s) 46, 76, 372
MboII GAAGA 4 cut(s) 71, 274, 318, 400
MhlI GDGCHC 1 cut(s) 123
MluCI AATT 3 cut(s) 183, 213, 313
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 3 cut(s) 29, 335, 408
Mph1103I ATGCAT 1 cut(s) 145
MseI TTAA 2 cut(s) 212, 258
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 1 cut(s) 104
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 104
NcoI CCATGG 1 cut(s) 178
NdeII GATC 3 cut(s) 46, 76, 372
NlaIII CATG 3 cut(s) 53, 147, 182
NmeAIII GCCGAG 1 cut(s) 142
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 147
Ppu21I YACGTR 1 cut(s) 230
PshBI ATTAAT 1 cut(s) 212
PsiI TTATAA 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 303
RsaI GTAC 3 cut(s) 64, 320, 395
RsaNI GTAC 3 cut(s) 63, 319, 394
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 2 cut(s) 212, 258
Sau3AI GATC 3 cut(s) 46, 76, 372
Sau96I GGNCC 1 cut(s) 303
ScrFI CCNGG 1 cut(s) 104
SduI GDGCHC 1 cut(s) 123
SetI ASST 5 cut(s) 34, 123, 232, 258, 294
SinI GGWCC 1 cut(s) 303
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 3 cut(s) 183, 213, 313
SsiI CCGC 1 cut(s) 277
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 1 cut(s) 102
StyI CCWWGG 1 cut(s) 178
TaiI ACGT 1 cut(s) 232
TaqI TCGA 3 cut(s) 21, 136, 371
TaqII GACCGA 1 cut(s) 291
TasI AATT 3 cut(s) 183, 213, 313
TatI WGTACW 1 cut(s) 62
Tru1I TTAA 2 cut(s) 212, 258
Tru9I TTAA 2 cut(s) 212, 258
TspDTI ATGAA 1 cut(s) 318
TspGWI ACGGA 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 303
VspI ATTAAT 1 cut(s) 212
XapI RAATTY 1 cut(s) 183
XceI RCATGY 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.