Rroxscaffold_7G00197190

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005565
Physical Location & Seq
Reverse (-)
41021107 .. 41084032
62926 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197190.1

Sequence Viewer

Length: 759 bp
ATGAACCTTGCTGTTTGGAGTTCCTCTGTTACCGGACACAAATATACTTGGTGGGAGATACCTAATGTACTCAAGAAGTGTAGTCAAAGCTGTTGGATTATTGATGATGAAAGATTGGGAGAAGCATCTGTTGAGGAAATAGTAGGTAGCAACATTCTTCCTATATGTCGTGGTGGACAAAACGATATCAAGCTGTACAGAATCGACGGAAAAAGGGGAGAACCCGAAGAGGAAGTGGGAAGAGTCGGGGAGAGGAGAGAGAAAGAGGGGTGGGTTTCCGGTTTTGGAAACCTACCCGGCTCACCGCCGCCGCTACAGGCTTCGCCGCCGTGCAAGGCCACCACAGACAGTGCGCAAGGACCATACGAAGCTAATGAAAGAAGTTTGGTGAAGATTGATGGCCGGAGGAGGAAGAAATCGCTTGGAGAAGGAGGAGGGCTATTTCCGGTCACAGCTTGTGCTCCAGTGGGTAGGGGCAAAGTTTTCGTGCAGCAGGTTAGAAGAAACAGAGCAGTGATCGAGTGGATCAGAGTGCTGATGGATGTTGGACGGTTGTTCGGAGAAAAGAAAGATTATATGAAATTGGTAAGTTTAATTGAAACACGGTTCTTTTGCAGATCTATACCTTCAGATTCTTCATCCAGAAGACGCTGTGTTGGTGGGTTTCGGGAGAGTGAGGATTGCAGGAAGGATGAGACTTGGGTGAGCCAGATAGAATTGTCAGGAAACTGTTGCAGCCTAGGGTCTATCTCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.09

Weight (kDa)

8.8

Isoelectric Point (pI)

62.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 484
AciI CCGC 4 cut(s) 305, 308, 311, 326
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 2 cut(s) 69, 197
AgsI TTSAA 1 cut(s) 599
AluBI AGCT 4 cut(s) 90, 193, 371, 455
AluI AGCT 4 cut(s) 90, 193, 371, 455
Alw21I GWGCWC 1 cut(s) 463
Alw26I GTCTC 1 cut(s) 689
AlwI GGATC 1 cut(s) 533
AoxI GGCC 2 cut(s) 336, 400
ApeKI GCWGC 2 cut(s) 490, 735
AspA2I CCTAGG 1 cut(s) 739
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 1 cut(s) 359
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 3 cut(s) 294, 400, 715
AvaII GGWCC 1 cut(s) 359
AvrII CCTAGG 1 cut(s) 739
BbsI GAAGAC 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 463
BbvI GCAGC 2 cut(s) 502, 747
BccI CCATC 2 cut(s) 392, 532
BceAI ACGGC 1 cut(s) 313
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 689
BfaI CTAG 1 cut(s) 740
BfmI CTRYAG 1 cut(s) 314
BfuAI ACCTGC 1 cut(s) 484
BglII AGATCT 1 cut(s) 617
BisI GCNGC 5 cut(s) 308, 311, 326, 491, 736
BlnI CCTAGG 1 cut(s) 739
BlsI GCNGC 5 cut(s) 309, 312, 327, 492, 737
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 1 cut(s) 134
BpiI GAAGAC 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 297
BsaJI CCNNGG 1 cut(s) 739
BsaWI WCCGGW 3 cut(s) 32, 278, 445
Bse1I ACTGG 1 cut(s) 464
BseDI CCNNGG 1 cut(s) 739
BseGI GGATG 3 cut(s) 547, 638, 697
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 3 cut(s) 268, 421, 447
BseXI GCAGC 2 cut(s) 502, 747
BsgI GTGCAG 1 cut(s) 509
BshFI GGCC 2 cut(s) 338, 402
BsiHKAI GWGCWC 1 cut(s) 463
BsiSI CCGG 5 cut(s) 33, 279, 297, 403, 446
BsmAI GTCTC 1 cut(s) 689
BsnI GGCC 2 cut(s) 338, 402
Bsp1286I GDGCHC 1 cut(s) 463
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 3 cut(s) 516, 525, 617
BspACI CCGC 4 cut(s) 305, 308, 311, 326
BspANI GGCC 2 cut(s) 338, 402
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 1 cut(s) 533
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 1 cut(s) 464
BssECI CCNNGG 1 cut(s) 739
BssMI GATC 3 cut(s) 516, 525, 617
BssT1I CCWWGG 1 cut(s) 739
Bst4CI ACNGT 4 cut(s) 350, 552, 606, 731
Bst6I CTCTTC 2 cut(s) 222, 235
BstAUI TGTACA 1 cut(s) 195
BstF5I GGATG 3 cut(s) 547, 638, 697
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 3 cut(s) 519, 528, 620
BstMAI GTCTC 1 cut(s) 689
BstMBI GATC 3 cut(s) 516, 525, 617
BstSCI CCNGG 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 314
BstV1I GCAGC 2 cut(s) 502, 747
BstV2I GAAGAC 1 cut(s) 652
BstX2I RGATCY 1 cut(s) 617
BstYI RGATCY 1 cut(s) 617
BsuRI GGCC 2 cut(s) 338, 402
BtsCI GGATG 3 cut(s) 547, 638, 697
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 3 cut(s) 355, 471, 519
BveI ACCTGC 1 cut(s) 484
CfoI GCGC 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 359
CseI GACGC 1 cut(s) 657
Csp6I GTAC 2 cut(s) 68, 196
CviQI GTAC 2 cut(s) 68, 196
DpnI GATC 3 cut(s) 518, 527, 619
DpnII GATC 3 cut(s) 516, 525, 617
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 2 cut(s) 222, 235
EarI CTCTTC 2 cut(s) 222, 235
Eco130I CCWWGG 1 cut(s) 739
Eco32I GATATC 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 612
EcoRV GATATC 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 739
ErhI CCWWGG 1 cut(s) 739
FaiI YATR 7 cut(s) 45, 164, 166, 364, 576, 578, 623
Fnu4HI GCNGC 5 cut(s) 308, 311, 326, 491, 736
FokI GGATG 3 cut(s) 554, 625, 704
Fsp4HI GCNGC 5 cut(s) 308, 311, 326, 491, 736
FspBI CTAG 1 cut(s) 740
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 1 cut(s) 354
GluI GCNGC 5 cut(s) 308, 311, 326, 491, 736
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 2 cut(s) 338, 402
HapII CCGG 5 cut(s) 33, 279, 297, 403, 446
HgaI GACGC 1 cut(s) 657
HhaI GCGC 1 cut(s) 355
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 3 cut(s) 201, 243, 632
HpaII CCGG 5 cut(s) 33, 279, 297, 403, 446
HphI GGTGA 3 cut(s) 294, 400, 715
Hpy166II GTNNAC 1 cut(s) 176
Hpy188I TCNGA 3 cut(s) 530, 560, 631
Hpy188III TCNNGA 4 cut(s) 73, 642, 668, 723
Hpy8I GTNNAC 1 cut(s) 176
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 3 cut(s) 422, 636, 682
HpyCH4III ACNGT 4 cut(s) 350, 552, 606, 731
HpyCH4V TGCA 5 cut(s) 333, 490, 615, 684, 735
HspAI GCGC 1 cut(s) 353
Kzo9I GATC 3 cut(s) 516, 525, 617
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 2 cut(s) 502, 747
LweI GCATC 1 cut(s) 134
MaeI CTAG 1 cut(s) 740
MaeIII GTNAC 2 cut(s) 28, 448
MalI GATC 3 cut(s) 518, 527, 619
MboI GATC 3 cut(s) 516, 525, 617
MboII GAAGA 8 cut(s) 149, 239, 252, 403, 424, 513, 627, 657
MflI RGATCY 1 cut(s) 617
MhlI GDGCHC 1 cut(s) 463
MluCI AATT 4 cut(s) 581, 594, 716, 754
MlyI GAGTC 1 cut(s) 252
MmeI TCCRAC 2 cut(s) 74, 526
MseI TTAA 1 cut(s) 593
MspI CCGG 5 cut(s) 33, 279, 297, 403, 446
MspR9I CCNGG 1 cut(s) 297
NciI CCSGG 1 cut(s) 297
NdeII GATC 3 cut(s) 516, 525, 617
NmuCI GTSAC 1 cut(s) 448
NsbI TGCGCA 1 cut(s) 354
PfeI GAWTC 2 cut(s) 201, 632
PkrI GCNGC 5 cut(s) 309, 312, 327, 492, 737
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PspPI GGNCC 1 cut(s) 359
PsuI RGATCY 1 cut(s) 617
RsaI GTAC 2 cut(s) 69, 197
RsaNI GTAC 2 cut(s) 68, 196
SaqAI TTAA 1 cut(s) 593
SatI GCNGC 5 cut(s) 308, 311, 326, 491, 736
Sau3AI GATC 3 cut(s) 516, 525, 617
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 1 cut(s) 252
ScrFI CCNGG 1 cut(s) 297
SduI GDGCHC 1 cut(s) 463
SfaNI GCATC 1 cut(s) 134
SfcI CTRYAG 1 cut(s) 314
SinI GGWCC 1 cut(s) 359
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 4 cut(s) 581, 594, 716, 754
SsiI CCGC 4 cut(s) 305, 308, 311, 326
SspMI CTAG 1 cut(s) 740
StyD4I CCNGG 1 cut(s) 295
StyI CCWWGG 1 cut(s) 739
TaaI ACNGT 4 cut(s) 350, 552, 606, 731
TaqI TCGA 2 cut(s) 204, 519
TasI AATT 4 cut(s) 581, 594, 716, 754
TatI WGTACW 2 cut(s) 67, 195
TauI GCSGC 3 cut(s) 310, 313, 328
TfiI GAWTC 2 cut(s) 201, 632
Tru1I TTAA 1 cut(s) 593
Tru9I TTAA 1 cut(s) 593
TscAI CASTG 3 cut(s) 355, 471, 519
TseFI GTSAC 1 cut(s) 448
TseI GCWGC 2 cut(s) 490, 735
Tsp45I GTSAC 1 cut(s) 448
TspDTI ATGAA 5 cut(s) 17, 123, 390, 593, 627
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 3 cut(s) 355, 471, 519
VpaK11BI GGWCC 1 cut(s) 359
XmaJI CCTAGG 1 cut(s) 739
XspI CTAG 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.