Rroxscaffold_7G00197200

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005565
Physical Location & Seq
Reverse (-)
41090272 .. 41091337
1066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197200.1

Sequence Viewer

Length: 759 bp
ATGCTTCCTCCTCACACCCCCATCCTCATCCAAGCTCACCATCTCCTTAGTGTGCTTCTACTTCGCACCGGGAGCTATGGCTACCACCGGCGTAACTGGTCAGAGATCCGGGGACTGGATAAGCTGACTGAACTCAGTACTTCTCGAAATGGCAACAGAAATTGCAAAGGCTTCTCCGGCGACCGTCAATGGCGACGATGTGGGGGCACTCTACGGAGTCGTTCGAGCTCTGCCCAGCCACGCTGTGATCGAGTAGTCTCAATAGGGTTTGCTTATTGTGAGGATAAGGATGAGATGGTGGTGAGAGAGGAGGGTACGGATGGGTTGGCCGGTTACATTTCCGACCTGATTAGGGAACAGGAATTGAGTTTGACGTTTTTTCTCTTGAAGTGGCTATACCATCAGTTATCCTGCAAAAGAAGAAAAACAGGCACACATAATGGTTTGGACACAAGAGATGATGACATTAAATGCTCTGGATTTTCAACAAGTGAAGCTGCGAGTCTGCAAGCTATTGAATTGTCAAAATGTCTGAGTTACGTCTGGAGCAGACCAATGAACCTTGCTGTTTGGAGTTCCTCTGTTACCGGACACAAATATACTTGGTGGGAGATACCTAATGTACTCAAGAAGTGTAGTCAAAGCTGTTGGATTATTGATGATGAAAGATTGGGAGAAGCATCTGTTGAGGAAATAGTAGGTAGCAACATTCTTCCTATATGTCGTGGTGGACAAAACGATATCAAGCTGTACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.68

Weight (kDa)

8.66

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 4 cut(s) 139, 316, 624, 752
AfiI CCNNNNNNNGG 2 cut(s) 115, 352
AgsI TTSAA 3 cut(s) 388, 486, 518
AluBI AGCT 8 cut(s) 35, 75, 124, 228, 497, 512, 645, 748
AluI AGCT 8 cut(s) 35, 75, 124, 228, 497, 512, 645, 748
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 1 cut(s) 100
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 497
AsuC2I CCSGG 2 cut(s) 70, 110
AsuHPI GGTGA 2 cut(s) 29, 313
BaeGI GKGCMC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 230
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
Bbv12I GWGCWC 1 cut(s) 230
BbvI GCAGC 1 cut(s) 484
BccI CCATC 5 cut(s) 29, 48, 289, 314, 408
BcnI CCSGG 2 cut(s) 70, 110
BcoDI GTCTC 1 cut(s) 262
BisI GCNGC 1 cut(s) 498
BlsI GCNGC 1 cut(s) 499
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 2 cut(s) 70, 110
BmrFI CCNGG 2 cut(s) 70, 110
BmsI GCATC 1 cut(s) 689
BpmI CTGGAG 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 2 cut(s) 70, 110
BsaJI CCNNGG 1 cut(s) 109
BsaWI WCCGGW 1 cut(s) 587
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 352
Bse118I RCCGGY 2 cut(s) 87, 329
Bse1I ACTGG 2 cut(s) 101, 120
BseDI CCNNGG 1 cut(s) 109
BseGI GGATG 4 cut(s) 21, 27, 295, 325
BseLI CCNNNNNNNGG 2 cut(s) 115, 352
BseMII CTCAG 2 cut(s) 148, 524
BseNI ACTGG 2 cut(s) 101, 120
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 1 cut(s) 234
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 230
BsiSI CCGG 6 cut(s) 69, 88, 109, 177, 330, 588
BslFI GGGAC 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 115, 352
BsmAI GTCTC 1 cut(s) 262
BsmFI GGGAC 1 cut(s) 126
BsnI GGCC 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 209, 230
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 2 cut(s) 105, 247
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 147, 525
BspPI GGATC 1 cut(s) 100
BsrFI RCCGGY 2 cut(s) 87, 329
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 2 cut(s) 101, 120
BssAI RCCGGY 2 cut(s) 87, 329
BssECI CCNNGG 1 cut(s) 109
BssMI GATC 2 cut(s) 105, 247
Bst4CI ACNGT 1 cut(s) 185
BstAUI TGTACA 1 cut(s) 750
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 3 cut(s) 47, 134, 533
BstF5I GGATG 4 cut(s) 21, 27, 295, 325
BstKTI GATC 2 cut(s) 108, 250
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 2 cut(s) 105, 247
BstMCI CGRYCG 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 72, 177
BstSCI CCNGG 2 cut(s) 68, 108
BstSLI GKGCMC 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 1 cut(s) 329
BtsCI GGATG 4 cut(s) 21, 27, 295, 325
Cac8I GCNNGC 1 cut(s) 510
Cfr10I RCCGGY 2 cut(s) 87, 329
Csp6I GTAC 4 cut(s) 138, 315, 623, 751
CviQI GTAC 4 cut(s) 138, 315, 623, 751
DdeI CTNAG 3 cut(s) 47, 134, 533
DpnI GATC 2 cut(s) 107, 249
DpnII GATC 2 cut(s) 105, 247
DraIII CACNNNGTG 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 230
Eco32I GATATC 1 cut(s) 742
Eco53kI GAGCTC 1 cut(s) 228
EcoICRI GAGCTC 1 cut(s) 228
EcoRV GATATC 1 cut(s) 742
EcoT38I GRGCYC 1 cut(s) 230
FaiI YATR 6 cut(s) 78, 397, 438, 600, 719, 721
FaqI GGGAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 498
FokI GGATG 4 cut(s) 8, 14, 302, 332
FriOI GRGCYC 1 cut(s) 230
Fsp4HI GCNGC 1 cut(s) 498
GluI GCNGC 1 cut(s) 498
GsaI CCCAGC 1 cut(s) 238
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 1 cut(s) 329
HapII CCGG 6 cut(s) 69, 88, 109, 177, 330, 588
HinfI GANTC 2 cut(s) 217, 502
HpaII CCGG 6 cut(s) 69, 88, 109, 177, 330, 588
HphI GGTGA 2 cut(s) 29, 313
Hpy166II GTNNAC 1 cut(s) 731
Hpy188I TCNGA 3 cut(s) 103, 343, 534
Hpy188III TCNNGA 5 cut(s) 144, 385, 477, 544, 628
Hpy8I GTNNAC 1 cut(s) 731
Hpy99I CGWCG 1 cut(s) 198
HpyCH4III ACNGT 1 cut(s) 185
HpyCH4IV ACGT 2 cut(s) 374, 540
HpyCH4V TGCA 3 cut(s) 165, 414, 508
HpyF10VI GCNNNNNNNGC 2 cut(s) 72, 177
HpyF3I CTNAG 3 cut(s) 47, 134, 533
HpySE526I ACGT 2 cut(s) 374, 540
Kzo9I GATC 2 cut(s) 105, 247
LmnI GCTCC 2 cut(s) 72, 546
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 1 cut(s) 689
MaeII ACGT 2 cut(s) 374, 540
MaeIII GTNAC 4 cut(s) 92, 332, 536, 583
MalI GATC 2 cut(s) 107, 249
MboI GATC 2 cut(s) 105, 247
MboII GAAGA 2 cut(s) 432, 704
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 2 cut(s) 209, 230
MluCI AATT 3 cut(s) 160, 362, 518
MlyI GAGTC 2 cut(s) 226, 511
MmeI TCCRAC 2 cut(s) 366, 629
MnlI CCTC 8 cut(s) 18, 21, 35, 274, 301, 304, 589, 682
MseI TTAA 1 cut(s) 468
MspI CCGG 6 cut(s) 69, 88, 109, 177, 330, 588
MspR9I CCNGG 2 cut(s) 70, 110
MwoI GCNNNNNNNGC 2 cut(s) 72, 177
NciI CCSGG 2 cut(s) 70, 110
NdeII GATC 2 cut(s) 105, 247
PcsI WCGNNNNNNNCGW 1 cut(s) 247
PkrI GCNGC 1 cut(s) 499
PleI GAGTC 2 cut(s) 225, 510
PpsI GAGTC 2 cut(s) 225, 510
Psp124BI GAGCTC 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 4 cut(s) 139, 316, 624, 752
RsaNI GTAC 4 cut(s) 138, 315, 623, 751
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 1 cut(s) 498
Sau3AI GATC 2 cut(s) 105, 247
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 2 cut(s) 226, 511
ScrFI CCNGG 2 cut(s) 70, 110
SduI GDGCHC 2 cut(s) 209, 230
SfaNI GCATC 1 cut(s) 689
SgrAI CRCCGGYG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 3 cut(s) 160, 362, 518
SstI GAGCTC 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 68, 108
TaaI ACNGT 1 cut(s) 185
TaiI ACGT 2 cut(s) 377, 543
TaqI TCGA 3 cut(s) 145, 224, 250
TasI AATT 3 cut(s) 160, 362, 518
TatI WGTACW 3 cut(s) 137, 622, 750
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TseI GCWGC 1 cut(s) 497
TspDTI ATGAA 2 cut(s) 572, 678
TspGWI ACGGA 2 cut(s) 229, 332
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.