Rroxscaffold_6G00404140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005565
Physical Location & Seq
Reverse (-)
26602686 .. 26631077
28392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404140.1

Sequence Viewer

Length: 636 bp
ATGCTTGCACATGGCTATGTAAGTACAAGTCCCCTAAGTCCACAATCAAGGAGGGCAATCTTGGTTGGGGAGTTGCCTAGCGCTTCGAAACGTTACTTCCGCCGGTCTTGCAAAAGCATAATTAGCTCCTTTATTCCTCGGTTGTTTGCAACATTAAGTCCTGGACGAACATTTGAATTAAAACACCGTGAGGTGCGGTCGTCGGGGAATCCCTTGACACTGACTTCGAATCAAACAACGTGGATGAGGAGGGCACCAACCTCGTCACAAGGTTCTTTTCTTTGCCTTTCGGAAAAGGACTTCTTGCCTTACGGAGATTTCTGCTGCGGGACCCGCACCGCGAGGCCCGGTGTCAGCGCAGCTCCGGTGCTGACCGTATCGAAGCGGTGGAGGGACATGTTCAAGAAGAAGAGTGAGAGTAGTGAGAAAGAGAAGGAGAAGAAAAAGGAGCGGAAGAAGGAGAACCGTTTTTGCGGAGCAGATCCGCCTGATGGCCGTCTGACGGATCCCCATCGTGTGCTCTTCGGTATTGACAATGTCATTGGGAATGGAAACCTCTCGGATTTAGCTCGACAAGGATTGTTTGGTGGAAGTGAGTCCTTTTTGGATGTTGAAAGTGCTCGATTGAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

211

Amino Acids

23.53

Weight (kDa)

10.06

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AccBSI CCGCTC 1 cut(s) 451
AccII CGCG 1 cut(s) 341
AciI CCGC 9 cut(s) 100, 196, 327, 334, 339, 385, 451, 474, 485
AclI AACGTT 1 cut(s) 91
AclWI GGATC 3 cut(s) 476, 500, 513
AcoI YGGCCR 1 cut(s) 493
AfaI GTAC 1 cut(s) 25
AfeI AGCGCT 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 491, 502
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 4 cut(s) 176, 403, 614, 628
AjnI CCWGG 1 cut(s) 160
AluBI AGCT 3 cut(s) 126, 362, 569
AluI AGCT 3 cut(s) 126, 362, 569
Alw21I GWGCWC 2 cut(s) 522, 622
AlwI GGATC 3 cut(s) 476, 500, 513
Aor51HI AGCGCT 1 cut(s) 82
AoxI GGCC 2 cut(s) 344, 493
ApeKI GCWGC 2 cut(s) 324, 359
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
AspLEI GCGC 2 cut(s) 83, 359
AspS9I GGNCC 2 cut(s) 330, 345
AsuC2I CCSGG 1 cut(s) 348
AsuII TTCGAA 2 cut(s) 86, 227
AvaII GGWCC 1 cut(s) 330
BaeGI GKGCMC 1 cut(s) 256
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 522, 622
BbvI GCAGC 2 cut(s) 311, 371
BccI CCATC 2 cut(s) 485, 519
BceAI ACGGC 1 cut(s) 480
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 1 cut(s) 162
BcnI CCSGG 1 cut(s) 348
BfaI CTAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 2 cut(s) 325, 360
BlsI GCNGC 2 cut(s) 326, 361
Bme1390I CCNGG 2 cut(s) 162, 348
Bme18I GGWCC 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 330, 345
BmiI GGNNCC 4 cut(s) 255, 331, 332, 507
BmrFI CCNGG 2 cut(s) 162, 348
Bpu14I TTCGAA 2 cut(s) 86, 227
BpuMI CCSGG 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 1 cut(s) 137
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 2 cut(s) 491, 502
Bse118I RCCGGY 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 2 cut(s) 249, 613
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 491, 502
BseRI GAGGAG 1 cut(s) 262
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 2 cut(s) 311, 371
Bsh1236I CGCG 1 cut(s) 341
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 2 cut(s) 346, 495
BshNI GGYRCC 1 cut(s) 253
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 522, 622
BsiSI CCGG 3 cut(s) 103, 348, 365
BslFI GGGAC 3 cut(s) 15, 343, 407
BslI CCNNNNNNNGG 2 cut(s) 491, 502
BsmFI GGGAC 3 cut(s) 15, 343, 407
BsnI GGCC 2 cut(s) 346, 495
Bsp119I TTCGAA 2 cut(s) 86, 227
Bsp1286I GDGCHC 3 cut(s) 256, 522, 622
Bsp143I GATC 2 cut(s) 481, 505
BspACI CCGC 9 cut(s) 100, 196, 327, 334, 339, 385, 451, 474, 485
BspANI GGCC 2 cut(s) 346, 495
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 4 cut(s) 255, 331, 332, 507
BspPI GGATC 3 cut(s) 476, 500, 513
BspQI GCTCTTC 1 cut(s) 527
BspT104I TTCGAA 2 cut(s) 86, 227
BspT107I GGYRCC 1 cut(s) 253
BsrBI CCGCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 102
BssAI RCCGGY 1 cut(s) 102
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 2 cut(s) 481, 505
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 3 cut(s) 188, 376, 467
Bst6I CTCTTC 2 cut(s) 404, 527
BstBI TTCGAA 2 cut(s) 86, 227
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 2 cut(s) 249, 613
BstFNI CGCG 1 cut(s) 341
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 359
BstKTI GATC 2 cut(s) 484, 508
BstMBI GATC 2 cut(s) 481, 505
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 3 cut(s) 108, 123, 333
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 160, 346
BstSLI GKGCMC 1 cut(s) 256
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 311, 371
BstX2I RGATCY 2 cut(s) 481, 505
BstYI RGATCY 2 cut(s) 481, 505
BsuRI GGCC 2 cut(s) 346, 495
BtsCI GGATG 2 cut(s) 249, 613
BtsIMutI CAGTG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 83, 359
Cfr10I RCCGGY 1 cut(s) 102
Cfr13I GGNCC 2 cut(s) 330, 345
Csp6I GTAC 1 cut(s) 24
CspCI CAANNNNNGTGG 2 cut(s) 221, 256
CviAII CATG 2 cut(s) 11, 397
CviJI RGCY 6 cut(s) 15, 126, 346, 362, 495, 569
CviKI_1 RGCY 6 cut(s) 15, 126, 346, 362, 495, 569
CviQI GTAC 1 cut(s) 24
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 483, 507
DpnII GATC 2 cut(s) 481, 505
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 404, 527
EarI CTCTTC 2 cut(s) 404, 527
EciI GGCGGA 2 cut(s) 89, 474
Eco47I GGWCC 1 cut(s) 330
Eco47III AGCGCT 1 cut(s) 82
EcoO109I RGGNCCY 1 cut(s) 330
EcoRII CCWGG 1 cut(s) 160
FaeI CATG 2 cut(s) 14, 400
FaiI YATR 4 cut(s) 12, 18, 119, 398
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 3 cut(s) 15, 343, 407
FatI CATG 2 cut(s) 10, 396
FauI CCCGC 2 cut(s) 320, 341
Fnu4HI GCNGC 2 cut(s) 325, 360
FokI GGATG 2 cut(s) 256, 620
Fsp4HI GCNGC 2 cut(s) 325, 360
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 82, 358
GluI GCNGC 2 cut(s) 325, 360
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 2 cut(s) 346, 495
HapII CCGG 3 cut(s) 103, 348, 365
HhaI GCGC 2 cut(s) 83, 359
Hin1II CATG 2 cut(s) 14, 400
Hin6I GCGC 2 cut(s) 81, 357
HinP1I GCGC 2 cut(s) 81, 357
HinfI GANTC 3 cut(s) 208, 229, 596
HpaII CCGG 3 cut(s) 103, 348, 365
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 3 cut(s) 292, 501, 562
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 1 cut(s) 41
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 427, 451
HpyCH4III ACNGT 3 cut(s) 188, 376, 467
HpyCH4IV ACGT 2 cut(s) 91, 239
HpyCH4V TGCA 3 cut(s) 8, 111, 149
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 123, 333
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 91, 239
Hsp92II CATG 2 cut(s) 14, 400
HspAI GCGC 2 cut(s) 81, 357
KflI GGGWCCC 1 cut(s) 330
Kzo9I GATC 2 cut(s) 481, 505
LguI GCTCTTC 1 cut(s) 527
LmnI GCTCC 4 cut(s) 131, 367, 448, 476
LpnPI CCDG 6 cut(s) 116, 147, 174, 361, 378, 501
Lsp1109I GCAGC 2 cut(s) 311, 371
MaeI CTAG 1 cut(s) 78
MaeII ACGT 2 cut(s) 91, 239
MaeIII GTNAC 2 cut(s) 92, 264
MalI GATC 2 cut(s) 483, 507
MbiI CCGCTC 1 cut(s) 451
MboI GATC 2 cut(s) 481, 505
MboII GAAGA 5 cut(s) 418, 421, 451, 466, 514
MflI RGATCY 2 cut(s) 481, 505
MhlI GDGCHC 3 cut(s) 256, 522, 622
MluCI AATT 2 cut(s) 120, 176
MlyI GAGTC 1 cut(s) 605
MnlI CCTC 9 cut(s) 45, 147, 184, 240, 243, 271, 336, 384, 566
MseI TTAA 2 cut(s) 155, 179
MslI CAYNNNNRTG 1 cut(s) 15
MspI CCGG 3 cut(s) 103, 348, 365
MspR9I CCNGG 2 cut(s) 162, 348
MvaI CCWGG 1 cut(s) 162
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 3 cut(s) 108, 123, 333
NciI CCSGG 1 cut(s) 348
NdeII GATC 2 cut(s) 481, 505
NlaIII CATG 2 cut(s) 14, 400
NlaIV GGNNCC 4 cut(s) 255, 331, 332, 507
NmuCI GTSAC 1 cut(s) 264
NspI RCATGY 1 cut(s) 400
NspV TTCGAA 2 cut(s) 86, 227
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 527
PfeI GAWTC 2 cut(s) 208, 229
PflFI GACNNNGTC 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 160
PkrI GCNGC 2 cut(s) 326, 361
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PpuMI RGGWCCY 1 cut(s) 330
PscI ACATGT 1 cut(s) 396
Psp1406I AACGTT 1 cut(s) 91
Psp5II RGGWCCY 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 4 cut(s) 255, 331, 332, 507
PspPI GGNCC 2 cut(s) 330, 345
PspPPI RGGWCCY 1 cut(s) 330
PsuI RGATCY 2 cut(s) 481, 505
PsyI GACNNNGTC 1 cut(s) 536
RsaI GTAC 1 cut(s) 25
RsaNI GTAC 1 cut(s) 24
RseI CAYNNNNRTG 1 cut(s) 15
SapI GCTCTTC 1 cut(s) 527
SaqAI TTAA 2 cut(s) 155, 179
SatI GCNGC 2 cut(s) 325, 360
Sau3AI GATC 2 cut(s) 481, 505
Sau96I GGNCC 2 cut(s) 330, 345
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 2 cut(s) 162, 348
SduI GDGCHC 3 cut(s) 256, 522, 622
SetI ASST 9 cut(s) 94, 128, 195, 242, 263, 274, 364, 558, 571
SfuI TTCGAA 2 cut(s) 86, 227
SinI GGWCC 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 15
Sse9I AATT 2 cut(s) 120, 176
SsiI CCGC 9 cut(s) 100, 196, 327, 334, 339, 385, 451, 474, 485
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 160, 346
TaaI ACNGT 3 cut(s) 188, 376, 467
TaiI ACGT 2 cut(s) 94, 242
TaqI TCGA 5 cut(s) 86, 227, 380, 571, 622
TasI AATT 2 cut(s) 120, 176
TatI WGTACW 1 cut(s) 23
TfiI GAWTC 2 cut(s) 208, 229
Tru1I TTAA 2 cut(s) 155, 179
Tru9I TTAA 2 cut(s) 155, 179
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 2 cut(s) 324, 359
Tsp45I GTSAC 1 cut(s) 264
TspGWI ACGGA 2 cut(s) 327, 518
TspRI CASTG 1 cut(s) 225
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 330
XceI RCATGY 1 cut(s) 400
XspI CTAG 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.