Rroxscaffold_7G00183480

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005565
Physical Location & Seq
Forward (+)
22556590 .. 22559008
2419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00183480.1

Sequence Viewer

Length: 732 bp
ATGCACATGGCATTCACCAACTGTATGCGGGTATTGGCATCCCATCTCCAAGTTATTGTACCTGCCACTAGTCCCAATACTGATGGAATCCACATTAGTGTGTCGAAGGGTGTTGAGGTCAAGGATAGCATAATTAGAATAGGAGATGACTGCATTTCTATAGTTAGCAATTCTTCACGCATCCGGATCGTGAATATTGTCCGTGGCCCTGGTCATGGCATTAGCATCGGAAGCTTGGGAAAATCAAACTCTTGGTCACAAGTGCGTGATGTAATGGTTGATGCAGCATTCCTCTCTAACACCGAGAATGGAGTGAGGATCAAGACATGGCAGGGAGGTAGTGGTTTTGCCTCAAATGTTGCATTTCGGAATGTGGTCATGGACAACGTATCAAATCCAATTATAATTGATCAATATTACTGTGACTCCCGGTTGCCATGTTTAAATCAGGTGACCGATGGTTTGAGATGGTGGACGATCGTGGATCGTATTTTGGGCTGCTATGAAGACGCAGCGGGATTGGAGCGGGAGATTTTGCAAGTTAGAAGAACCGGAGCGGTGATCGTGCGGATCGGAGATCTATTGGATTATAGAAGGTTCGTATTAGAAGAAGTTCAGTTTCCGCGAGTTATAATCTTCGAGGAATGGGAACCTCTGGATTTAGCTCGACAAAGATTGTTTGGTGGAAGTGAGTCCTTTTTGGATGTTGAAAGTGCTCGATTGAAAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0009505 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

243

Amino Acids

27.12

Weight (kDa)

6.1

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 1 - 160 7e-52 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 404, 632
Acc36I ACCTGC 1 cut(s) 70
AccBSI CCGCTC 2 cut(s) 526, 557
AccII CGCG 1 cut(s) 625
AccIII TCCGGA 1 cut(s) 183
AciI CCGC 6 cut(s) 28, 515, 526, 557, 568, 623
AclWI GGATC 4 cut(s) 194, 326, 492, 578
AfaI GTAC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 2 cut(s) 710, 724
AhlI ACTAGT 1 cut(s) 68
AjnI CCWGG 1 cut(s) 208
AleI CACNNNNGTG 1 cut(s) 96
AluBI AGCT 2 cut(s) 234, 665
AluI AGCT 2 cut(s) 234, 665
Alw21I GWGCWC 1 cut(s) 718
AlwI GGATC 4 cut(s) 194, 326, 492, 578
Aor13HI TCCGGA 1 cut(s) 183
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 284, 498, 512
ArsI GACNNNNNNTTYG 4 cut(s) 239, 271, 445, 477
Asp700I GAANNNNTTC 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 3 cut(s) 7, 463, 571
BbsI GAAGAC 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 718
BbvI GCAGC 3 cut(s) 296, 485, 524
BccI CCATC 4 cut(s) 51, 77, 452, 462
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 208, 242
BciT130I CCWGG 1 cut(s) 210
BclI TGATCA 1 cut(s) 409
BcnI CCSGG 1 cut(s) 430
BcuI ACTAGT 1 cut(s) 68
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 159
BfuAI ACCTGC 1 cut(s) 70
BglII AGATCT 1 cut(s) 577
BisI GCNGC 3 cut(s) 285, 499, 513
BlsI GCNGC 3 cut(s) 286, 500, 514
Bme1390I CCNGG 2 cut(s) 210, 430
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 651
BmrFI CCNGG 2 cut(s) 210, 430
BmsI GCATC 4 cut(s) 47, 189, 234, 271
BpiI GAAGAC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 430
BsaJI CCNNGG 2 cut(s) 202, 208
BsaWI WCCGGW 2 cut(s) 183, 551
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 1 cut(s) 215
BseAI TCCGGA 1 cut(s) 183
BseBI CCWGG 1 cut(s) 210
BseDI CCNNGG 2 cut(s) 202, 208
BseGI GGATG 3 cut(s) 38, 180, 709
BseLI CCNNNNNNNGG 1 cut(s) 215
BseXI GCAGC 3 cut(s) 296, 485, 524
Bsh1236I CGCG 1 cut(s) 625
Bsh1285I CGRYCG 1 cut(s) 480
BshFI GGCC 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 718
BsiSI CCGG 3 cut(s) 184, 430, 552
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 2 cut(s) 11, 287
BsnI GGCC 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 718
Bsp13I TCCGGA 1 cut(s) 183
Bsp143I GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
BspACI CCGC 6 cut(s) 28, 515, 526, 557, 568, 623
BspANI GGCC 1 cut(s) 207
BspEI TCCGGA 1 cut(s) 183
BspFNI CGCG 1 cut(s) 625
BspLI GGNNCC 1 cut(s) 651
BspMI ACCTGC 1 cut(s) 70
BspPI GGATC 4 cut(s) 194, 326, 492, 578
BsrBI CCGCTC 2 cut(s) 526, 557
BssECI CCNNGG 2 cut(s) 202, 208
BssMI GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
Bst2UI CCWGG 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 23, 422
BstDSI CCRYGG 1 cut(s) 202
BstEII GGTNACC 1 cut(s) 451
BstF5I GGATG 3 cut(s) 38, 180, 709
BstFNI CGCG 1 cut(s) 625
BstKTI GATC 8 cut(s) 189, 321, 412, 480, 487, 564, 573, 580
BstMBI GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
BstMCI CGRYCG 1 cut(s) 480
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstNI CCWGG 1 cut(s) 210
BstPI GGTNACC 1 cut(s) 451
BstSCI CCNGG 2 cut(s) 208, 428
BstSFI CTRYAG 1 cut(s) 159
BstUI CGCG 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 296, 485, 524
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 577
BstYI RGATCY 1 cut(s) 577
BsuRI GGCC 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 202
BtsCI GGATG 3 cut(s) 38, 180, 709
BveI ACCTGC 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 206
CseI GACGC 1 cut(s) 518
Csp6I GTAC 1 cut(s) 59
CviAII CATG 5 cut(s) 7, 215, 327, 379, 438
CviJI RGCY 4 cut(s) 207, 234, 498, 665
CviKI_1 RGCY 4 cut(s) 207, 234, 498, 665
CviQI GTAC 1 cut(s) 59
DpnI GATC 8 cut(s) 188, 320, 411, 479, 486, 563, 572, 579
DpnII GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
DraI TTTAAA 1 cut(s) 444
Eco91I GGTNACC 1 cut(s) 451
EcoO65I GGTNACC 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 208
FaeI CATG 5 cut(s) 10, 218, 330, 382, 441
FaqI GGGAC 1 cut(s) 57
FatI CATG 5 cut(s) 6, 214, 326, 378, 437
FauI CCCGC 3 cut(s) 21, 508, 519
FbaI TGATCA 1 cut(s) 409
Fnu4HI GCNGC 3 cut(s) 285, 499, 513
FokI GGATG 3 cut(s) 25, 167, 716
Fsp4HI GCNGC 3 cut(s) 285, 499, 513
FspBI CTAG 1 cut(s) 69
GluI GCNGC 3 cut(s) 285, 499, 513
HaeIII GGCC 1 cut(s) 207
HapII CCGG 3 cut(s) 184, 430, 552
HgaI GACGC 1 cut(s) 518
Hin1II CATG 5 cut(s) 10, 218, 330, 382, 441
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 3 cut(s) 87, 425, 692
HpaII CCGG 3 cut(s) 184, 430, 552
HphI GGTGA 3 cut(s) 7, 463, 571
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 3 cut(s) 230, 369, 575
Hpy188III TCNNGA 4 cut(s) 184, 190, 322, 656
Hpy8I GTNNAC 1 cut(s) 474
HpyAV CCTTC 2 cut(s) 100, 588
HpyCH4III ACNGT 2 cut(s) 23, 422
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 5 cut(s) 4, 153, 284, 362, 538
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 5 cut(s) 10, 218, 330, 382, 441
Kpn2I TCCGGA 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
LmnI GCTCC 2 cut(s) 523, 554
LpnPI CCDG 9 cut(s) 75, 195, 197, 222, 317, 434, 443, 565, 641
Lsp1109I GCAGC 3 cut(s) 296, 485, 524
LweI GCATC 4 cut(s) 47, 189, 234, 271
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 255, 422, 451
MalI GATC 8 cut(s) 188, 320, 411, 479, 486, 563, 572, 579
MbiI CCGCTC 2 cut(s) 526, 557
MboI GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
MboII GAAGA 5 cut(s) 165, 518, 558, 620, 628
MflI RGATCY 1 cut(s) 577
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 4 cut(s) 132, 169, 399, 405
MlyI GAGTC 2 cut(s) 419, 701
MnlI CCTC 7 cut(s) 109, 302, 309, 329, 361, 634, 663
MroI TCCGGA 1 cut(s) 183
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 1 cut(s) 443
MslI CAYNNNNRTG 2 cut(s) 96, 98
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 3 cut(s) 184, 430, 552
MspR9I CCNGG 2 cut(s) 210, 430
Mva1269I GAATGC 2 cut(s) 11, 287
MvaI CCWGG 1 cut(s) 210
MvnI CGCG 1 cut(s) 625
MwoI GCNNNNNNNGC 1 cut(s) 231
NciI CCSGG 1 cut(s) 430
NdeII GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
NlaIII CATG 5 cut(s) 10, 218, 330, 382, 441
NlaIV GGNNCC 1 cut(s) 651
NmuCI GTSAC 3 cut(s) 255, 422, 451
OliI CACNNNNGTG 1 cut(s) 96
PctI GAATGC 2 cut(s) 11, 287
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 1 cut(s) 87
PkrI GCNGC 3 cut(s) 286, 500, 514
Ple19I CGATCG 1 cut(s) 480
PleI GAGTC 2 cut(s) 419, 700
PpsI GAGTC 2 cut(s) 419, 700
PsiI TTATAA 2 cut(s) 404, 632
Psp6I CCWGG 1 cut(s) 208
PspEI GGTNACC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 651
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 577
PvuI CGATCG 1 cut(s) 480
RsaI GTAC 1 cut(s) 60
RsaNI GTAC 1 cut(s) 59
RseI CAYNNNNRTG 2 cut(s) 96, 98
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 3 cut(s) 285, 499, 513
Sau3AI GATC 8 cut(s) 186, 318, 409, 477, 484, 561, 570, 577
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 2 cut(s) 419, 701
ScrFI CCNGG 2 cut(s) 210, 430
SduI GDGCHC 1 cut(s) 718
SetI ASST 9 cut(s) 64, 120, 236, 340, 390, 453, 599, 655, 667
SfaNI GCATC 4 cut(s) 47, 189, 234, 271
SfcI CTRYAG 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 96, 98
SpeI ACTAGT 1 cut(s) 68
Sse9I AATT 4 cut(s) 132, 169, 399, 405
SsiI CCGC 6 cut(s) 28, 515, 526, 557, 568, 623
SspI AATATT 2 cut(s) 196, 416
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 208, 428
TaaI ACNGT 2 cut(s) 23, 422
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 104, 639, 667, 718
TaqII GACCGA 1 cut(s) 470
TasI AATT 4 cut(s) 132, 169, 399, 405
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TseFI GTSAC 3 cut(s) 255, 422, 451
TseI GCWGC 3 cut(s) 284, 498, 512
Tsp45I GTSAC 3 cut(s) 255, 422, 451
TspDTI ATGAA 1 cut(s) 519
TspGWI ACGGA 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.