Rroxscaffold_7G00200850

Phosphoenolpyruvate carboxykinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005565
Physical Location & Seq
Reverse (-)
48298178 .. 48303090
4913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00200850.1

Sequence Viewer

Length: 729 bp
ATGGACTCGAAGATGGAAAACGGTTTGGAGATGGAGTCATGCCTGGGTCTTCAACTAGGAGGAAACTCTCAATATTTGAAGCTTGAAATTTGCCATATTGCTTCTCTTTGTGAGAAATTGGAGTTGGAGACACCCACCCGTGCAGTCTCTCATGATTCTCGTCCTTTAACAGTTTATGTGGCTACTTCTATAAGTCCAGACATTGGTAACATTATTGGCCTTGAATTAAAAGGAGATAATGCACTTATTGCTGCTGATATTGAGGGTTCTTCACTTGTTTTATGTGGCAATGCATTCTCGGATTCAAATGCAACTAAAGAAGCACTAGTTGTCTTGTCCGGATCGTTATATTTTCCCGAAGAGGTGACTGATGGTTTGAGATGGTGGACGATCGTGGATCGTATTTTGGGCTGCTTTGAAGACGCGGCGGGATTGGAGCGGGAGATTTTGCAGGTTAGAAGAATCAGAGCAGTGATCATGCGGATCGGAGTTTATGGGATTATAGAAGATACCATTAAGGGTTGTGGAGATTTGGTAGTTCCAGGAGATGTAGGAGTTATTCGTTCTTCTGAAGGTGTTGCCCCATTGTTTCAAACTGGAAGCTCTAGTAGCTCAAATCTATTCAAGTTGCCACCTGCTGCAGTCCTTGCAACTTTTGACAGTAATAGAACCCCGTACGCAGTTGTTTGTTTCCATCATGCTTTGGATATGTTTTCTATGCTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEPCK_ATP

Protein Analysis

242

Amino Acids

26.09

Weight (kDa)

4.66

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 643
Acc36I ACCTGC 2 cut(s) 442, 643
AccB7I CCANNNNNTGG 1 cut(s) 203
AccBSI CCGCTC 1 cut(s) 439
AccII CGCG 1 cut(s) 425
AccIII TCCGGA 1 cut(s) 338
AciI CCGC 4 cut(s) 425, 428, 439, 481
AclWI GGATC 3 cut(s) 349, 405, 491
AcsI RAATTY 1 cut(s) 87
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 1 cut(s) 677
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 8 cut(s) 53, 79, 86, 224, 306, 419, 593, 625
AhlI ACTAGT 1 cut(s) 325
AjnI CCWGG 2 cut(s) 42, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AluBI AGCT 3 cut(s) 82, 603, 612
AluI AGCT 3 cut(s) 82, 603, 612
Alw26I GTCTC 2 cut(s) 122, 151
AlwI GGATC 3 cut(s) 349, 405, 491
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 3 cut(s) 251, 411, 638
ApoI RAATTY 1 cut(s) 87
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AsuHPI GGTGA 1 cut(s) 376
BbsI GAAGAC 2 cut(s) 41, 426
BbvI GCAGC 3 cut(s) 238, 398, 625
BccI CCATC 5 cut(s) 7, 25, 365, 375, 702
BciT130I CCWGG 2 cut(s) 44, 543
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 122, 151
BcuI ACTAGT 1 cut(s) 325
BfaI CTAG 3 cut(s) 56, 326, 606
BfmI CTRYAG 1 cut(s) 639
BfuAI ACCTGC 2 cut(s) 442, 643
BisI GCNGC 4 cut(s) 252, 412, 426, 639
BlsI GCNGC 4 cut(s) 253, 413, 427, 640
Bme1390I CCNGG 2 cut(s) 44, 543
BmrFI CCNGG 2 cut(s) 44, 543
BpiI GAAGAC 2 cut(s) 41, 426
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 4 cut(s) 20, 50, 519, 549
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 295
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 2 cut(s) 44, 543
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMI GCAATG 1 cut(s) 295
BseNI ACTGG 1 cut(s) 601
BseXI GCAGC 3 cut(s) 238, 398, 625
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 1 cut(s) 219
BsiEI CGRYCG 1 cut(s) 393
BsiSI CCGG 1 cut(s) 339
BsiWI CGTACG 1 cut(s) 675
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 2 cut(s) 122, 151
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 338
Bsp143I GATC 5 cut(s) 341, 390, 397, 474, 483
BspACI CCGC 4 cut(s) 425, 428, 439, 481
BspANI GGCC 1 cut(s) 219
BspEI TCCGGA 1 cut(s) 338
BspFNI CGCG 1 cut(s) 425
BspHI TCATGA 1 cut(s) 151
BspMAI CTGCAG 1 cut(s) 643
BspMI ACCTGC 2 cut(s) 442, 643
BspPI GGATC 3 cut(s) 349, 405, 491
BsrBI CCGCTC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 295
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 5 cut(s) 341, 390, 397, 474, 483
Bst2UI CCWGG 2 cut(s) 44, 543
Bst4CI ACNGT 3 cut(s) 23, 172, 662
Bst6I CTCTTC 1 cut(s) 354
BstAPI GCANNNNNTGC 2 cut(s) 248, 647
BstFNI CGCG 1 cut(s) 425
BstKTI GATC 5 cut(s) 344, 393, 400, 477, 486
BstMAI GTCTC 2 cut(s) 122, 151
BstMBI GATC 5 cut(s) 341, 390, 397, 474, 483
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 3 cut(s) 248, 609, 647
BstNI CCWGG 2 cut(s) 44, 543
BstSCI CCNGG 2 cut(s) 42, 541
BstSFI CTRYAG 1 cut(s) 639
BstUI CGCG 1 cut(s) 425
BstV1I GCAGC 3 cut(s) 238, 398, 625
BstV2I GAAGAC 2 cut(s) 41, 426
BsuRI GGCC 1 cut(s) 219
BtsI GCAGTG 1 cut(s) 477
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 2 cut(s) 442, 643
CciI TCATGA 1 cut(s) 151
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 676
CviAII CATG 4 cut(s) 39, 152, 478, 698
CviJI RGCY 6 cut(s) 82, 182, 219, 411, 603, 612
CviKI_1 RGCY 6 cut(s) 82, 182, 219, 411, 603, 612
CviQI GTAC 1 cut(s) 676
DpnI GATC 5 cut(s) 343, 392, 399, 476, 485
DpnII GATC 5 cut(s) 341, 390, 397, 474, 483
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Eco57I CTGAAG 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 42, 541
EcoT22I ATGCAT 1 cut(s) 295
FaeI CATG 4 cut(s) 42, 155, 481, 701
FatI CATG 4 cut(s) 38, 151, 477, 697
FauI CCCGC 2 cut(s) 421, 432
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 4 cut(s) 252, 412, 426, 639
Fsp4HI GCNGC 4 cut(s) 252, 412, 426, 639
FspBI CTAG 3 cut(s) 56, 326, 606
GluI GCNGC 4 cut(s) 252, 412, 426, 639
HaeIII GGCC 1 cut(s) 219
HapII CCGG 1 cut(s) 339
HgaI GACGC 1 cut(s) 431
Hin1II CATG 4 cut(s) 42, 155, 481, 701
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 5 cut(s) 5, 35, 155, 302, 462
HpaII CCGG 1 cut(s) 339
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 387
Hpy188I TCNGA 4 cut(s) 301, 467, 488, 571
Hpy188III TCNNGA 4 cut(s) 152, 197, 339, 356
Hpy8I GTNNAC 1 cut(s) 387
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 3 cut(s) 23, 172, 662
HpyCH4V TGCA 7 cut(s) 143, 242, 293, 311, 451, 641, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 609, 647
Hsp92II CATG 4 cut(s) 42, 155, 481, 701
Kpn2I TCCGGA 1 cut(s) 338
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 5 cut(s) 341, 390, 397, 474, 483
LmnI GCTCC 1 cut(s) 436
Lsp1109I GCAGC 3 cut(s) 238, 398, 625
MaeI CTAG 3 cut(s) 56, 326, 606
MaeIII GTNAC 2 cut(s) 206, 364
MalI GATC 5 cut(s) 343, 392, 399, 476, 485
MbiI CCGCTC 1 cut(s) 439
MboI GATC 5 cut(s) 341, 390, 397, 474, 483
MboII GAAGA 8 cut(s) 22, 41, 261, 371, 431, 471, 518, 558
MluCI AATT 3 cut(s) 87, 116, 224
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 3 cut(s) 53, 256, 355
Mph1103I ATGCAT 1 cut(s) 295
MroI TCCGGA 1 cut(s) 338
MseI TTAA 3 cut(s) 167, 227, 516
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 2 cut(s) 44, 543
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 2 cut(s) 44, 543
MvnI CGCG 1 cut(s) 425
MwoI GCNNNNNNNGC 3 cut(s) 248, 609, 647
NdeII GATC 5 cut(s) 341, 390, 397, 474, 483
NlaIII CATG 4 cut(s) 42, 155, 481, 701
NmuCI GTSAC 1 cut(s) 364
NsiI ATGCAT 1 cut(s) 295
PagI TCATGA 1 cut(s) 151
PaqCI CACCTGC 1 cut(s) 643
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 3 cut(s) 155, 302, 462
Pfl23II CGTACG 1 cut(s) 675
PflMI CCANNNNNTGG 1 cut(s) 203
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 4 cut(s) 253, 413, 427, 640
Ple19I CGATCG 1 cut(s) 393
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
Psp6I CCWGG 2 cut(s) 42, 541
PspGI CCWGG 2 cut(s) 42, 541
PspLI CGTACG 1 cut(s) 675
PstI CTGCAG 1 cut(s) 643
PvuI CGATCG 1 cut(s) 393
RsaI GTAC 1 cut(s) 677
RsaNI GTAC 1 cut(s) 676
SaqAI TTAA 3 cut(s) 167, 227, 516
SatI GCNGC 4 cut(s) 252, 412, 426, 639
Sau3AI GATC 5 cut(s) 341, 390, 397, 474, 483
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 2 cut(s) 44, 543
SetI ASST 7 cut(s) 84, 366, 456, 577, 605, 614, 637
SfcI CTRYAG 1 cut(s) 639
SpeI ACTAGT 1 cut(s) 325
Sse9I AATT 3 cut(s) 87, 116, 224
SsiI CCGC 4 cut(s) 425, 428, 439, 481
SspI AATATT 1 cut(s) 74
SspMI CTAG 3 cut(s) 56, 326, 606
StyD4I CCNGG 2 cut(s) 42, 541
TaaI ACNGT 3 cut(s) 23, 172, 662
TaqI TCGA 1 cut(s) 8
TasI AATT 3 cut(s) 87, 116, 224
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 3 cut(s) 155, 302, 462
Tru1I TTAA 3 cut(s) 167, 227, 516
Tru9I TTAA 3 cut(s) 167, 227, 516
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 3 cut(s) 251, 411, 638
Tsp45I GTSAC 1 cut(s) 364
TspRI CASTG 1 cut(s) 477
Van91I CCANNNNNTGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 87
XspI CTAG 3 cut(s) 56, 326, 606
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.