Rroxscaffold_5G00353670

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005565
Physical Location & Seq
Forward (+)
31553628 .. 31556774
3147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00353670.1

Sequence Viewer

Length: 1290 bp
ATGGCGGTCGTGGGGGATTTTGTTATAGGGAAAGCACTCGAAGCCCCGTATGCTGGTGTTGAAAAGCTGATTGCCAATAATCTACTCTTTAAATCCCTTTCCACCGACATCAAGTCTAAGCTAGACTCTTTGAGACCGATGATTCACAAGATGGGACAGCGTAACCAGCAATCCGATTGTCCCGAGGATCCGCATCGAGAGATTTACGAGAAGGCGCAGTGCTTGTCGGTAACTGCTCCCGAGGTTCGTACTTGGCACATCTACAAGAAGTACAAATACACCAACAAACTTCTCAAGTTGAATGAAATTCTAGAGAAGCAGTTAATTCTACCGAATGTGCGTGTAGCGATTGTTGTAGACCGAACGGTGGTAGCAGCGAAGGTAGAGAAGAGGAAGGATGGTTGTGGCAGTTCAAGTCTGAAATGGGAGCTGGAGGGAAGCAATGATCATGCCGAAATCAGTACACCAACCATTGATCTTCGTGGCAAAGCGCAAGGAGACCCGGCCTTACGAGCCGAGCTCATCCGTAAAGTTCGATATGCATGTGAGAAATGGGGCTTCTTTCAGGTAGTGAACCATGGAATTCTGTTGATGTCTTGGATAGGATTAATTACAAATGGAATACGTGAGTTCAATGAGCAGGATAGTGAGCTTAAGAAGAAGTTTTATAAGCGGTTTGGGACGAAGGTGTTTTATCGGTCCAACTACAATTCGTACCAATCTTCATCAGCTGATTGGAGGGCTACATTGGGCTGTTTTGTGGCTCGATCCACCCAAACCAGAAGAAGTACCCTCGGTTGTAGTTTTGGCAAGGTTGAAAAAAAATTAATCGAGAAAAGTGGGAGGCTCTGGATGCCGTGTCTCATCCTTCCTCCGACGGAAGAGCTTCGACGAACTAGACCCTCACTTCTTTGGAAGACAAGGCGACGAAATGTCAAATCTCCGGATGGTAGAATGCGACCGATATCACCATTATCGCTGCCCGAGAAGAGCAATCTCTGCTCGGCCTTGTTCTTCATCTTAGCCCGATACGACGGAAGCTATGAACTGGTACAGAAACTCATCGACCATGTCCCACAGCCACCATCGATTCGGCAGCCGCATACCGACCGCACTTTGAGGTTTCGGTTGCTCTGCGCGATTGGGATAGTCCGTACAGTTCGGGACTATGGGGAGAAGAAAGGTTTTGAGTCTCCGTTTCTTGTTGAGTGCGAGGGGGTGAATTATGGAGGAAACAGAAAGTGGAAGAGTGGGAAGCTTTGTGCCGACAAGAGGGAAAGAAAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

429

Amino Acids

49.22

Weight (kDa)

9.75

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 106 6.8e-10 Arabidopsis broad-spectrum mildew resistance protein RPW8
DIOX_N PF14226 156 - 252 1.1e-12 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccI GTMKAC 1 cut(s) 357
AccII CGCG 1 cut(s) 1139
AccIII TCCGGA 1 cut(s) 943
AciI CCGC 5 cut(s) 5, 191, 673, 1100, 1111
AclWI GGATC 3 cut(s) 182, 195, 762
AcsI RAATTY 2 cut(s) 306, 582
AfaI GTAC 7 cut(s) 250, 272, 463, 716, 790, 1053, 1156
AfiI CCNNNNNNNGG 3 cut(s) 53, 367, 1272
AflII CTTAAG 1 cut(s) 653
AgsI TTSAA 5 cut(s) 62, 301, 414, 634, 818
AhdI GACNNNNNGTC 2 cut(s) 112, 932
AluBI AGCT 9 cut(s) 67, 121, 430, 520, 652, 731, 886, 1041, 1258
AluI AGCT 9 cut(s) 67, 121, 430, 520, 652, 731, 886, 1041, 1258
Alw21I GWGCWC 1 cut(s) 522
Alw26I GTCTC 4 cut(s) 127, 492, 866, 1197
AlwI GGATC 3 cut(s) 182, 195, 762
Ama87I CYCGRG 3 cut(s) 182, 239, 983
Aor13HI TCCGGA 1 cut(s) 943
AoxI GGCC 2 cut(s) 504, 1005
ApeKI GCWGC 3 cut(s) 374, 979, 1096
ApoI RAATTY 2 cut(s) 306, 582
AseI ATTAAT 2 cut(s) 608, 827
Asp700I GAANNNNTTC 1 cut(s) 885
AspLEI GCGC 3 cut(s) 217, 493, 1139
AspS9I GGNCC 1 cut(s) 699
AsuC2I CCSGG 1 cut(s) 503
AsuHPI GGTGA 2 cut(s) 960, 1231
AvaI CYCGRG 3 cut(s) 182, 239, 983
AvaII GGWCC 1 cut(s) 699
BamHI GGATCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 522
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbsI GAAGAC 1 cut(s) 923
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 386, 966, 1108
BccI CCATC 4 cut(s) 145, 392, 941, 1093
BceAI ACGGC 1 cut(s) 841
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 1 cut(s) 503
BcoDI GTCTC 4 cut(s) 127, 492, 866, 1197
BfaI CTAG 3 cut(s) 122, 311, 897
BfrI CTTAAG 1 cut(s) 653
BisI GCNGC 4 cut(s) 375, 980, 1097, 1100
BlsI GCNGC 4 cut(s) 376, 981, 1098, 1101
Bme1390I CCNGG 1 cut(s) 503
Bme18I GGWCC 1 cut(s) 699
BmeRI GACNNNNNGTC 2 cut(s) 112, 932
BmeT110I CYCGRG 3 cut(s) 182, 239, 983
BmgT120I GGNCC 1 cut(s) 699
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 2 cut(s) 202, 843
BpiI GAAGAC 1 cut(s) 923
BplI GAGNNNNNCTC 2 cut(s) 504, 536
BpmI CTGGAG 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 503
Bsa29I ATCGAT 1 cut(s) 1088
BsaAI YACGTR 1 cut(s) 626
BsaBI GATNNNNATC 1 cut(s) 192
BsaI GGTCTC 2 cut(s) 127, 492
BsaJI CCNNGG 4 cut(s) 183, 240, 577, 793
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 367, 1272
Bse1I ACTGG 1 cut(s) 1053
Bse3DI GCAATG 1 cut(s) 448
Bse8I GATNNNNATC 1 cut(s) 192
BseAI TCCGGA 1 cut(s) 943
BseCI ATCGAT 1 cut(s) 1088
BseDI CCNNGG 4 cut(s) 183, 240, 577, 793
BseGI GGATG 5 cut(s) 403, 522, 858, 864, 952
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 3 cut(s) 53, 367, 1272
BseMI GCAATG 1 cut(s) 448
BseNI ACTGG 1 cut(s) 1053
BseXI GCAGC 3 cut(s) 386, 966, 1108
Bsh1236I CGCG 1 cut(s) 1139
Bsh1285I CGRYCG 3 cut(s) 9, 962, 1111
BshFI GGCC 2 cut(s) 506, 1007
BshVI ATCGAT 1 cut(s) 1088
BsiEI CGRYCG 3 cut(s) 9, 962, 1111
BsiHKAI GWGCWC 1 cut(s) 522
BsiHKCI CYCGRG 3 cut(s) 182, 239, 983
BsiSI CCGG 2 cut(s) 503, 944
BslFI GGGAC 5 cut(s) 165, 168, 694, 1058, 1178
BslI CCNNNNNNNGG 3 cut(s) 53, 367, 1272
BsmAI GTCTC 4 cut(s) 127, 492, 866, 1197
BsmFI GGGAC 5 cut(s) 165, 168, 694, 1058, 1178
BsmI GAATGC 1 cut(s) 960
BsnI GGCC 2 cut(s) 506, 1007
Bso31I GGTCTC 2 cut(s) 127, 492
BsoBI CYCGRG 3 cut(s) 182, 239, 983
Bsp1286I GDGCHC 1 cut(s) 522
Bsp13I TCCGGA 1 cut(s) 943
Bsp143I GATC 4 cut(s) 187, 445, 475, 767
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 5 cut(s) 5, 191, 673, 1100, 1111
BspANI GGCC 2 cut(s) 506, 1007
BspDI ATCGAT 1 cut(s) 1088
BspEI TCCGGA 1 cut(s) 943
BspFNI CGCG 1 cut(s) 1139
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 3 cut(s) 182, 195, 762
BspQI GCTCTTC 2 cut(s) 876, 983
BspTI CTTAAG 1 cut(s) 653
BspTNI GGTCTC 2 cut(s) 127, 492
BsrDI GCAATG 1 cut(s) 448
BsrI ACTGG 1 cut(s) 1053
BssECI CCNNGG 4 cut(s) 183, 240, 577, 793
BssMI GATC 4 cut(s) 187, 445, 475, 767
BssT1I CCWWGG 1 cut(s) 577
Bst4CI ACNGT 2 cut(s) 367, 1159
Bst6I CTCTTC 4 cut(s) 383, 876, 983, 1241
BstAFI CTTAAG 1 cut(s) 653
BstAPI GCANNNNNTGC 1 cut(s) 999
BstBAI YACGTR 1 cut(s) 626
BstDEI CTNAG 2 cut(s) 117, 1021
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 5 cut(s) 403, 522, 858, 864, 952
BstFNI CGCG 1 cut(s) 1139
BstHHI GCGC 3 cut(s) 217, 493, 1139
BstKTI GATC 4 cut(s) 190, 448, 478, 770
BstMAI GTCTC 4 cut(s) 127, 492, 866, 1197
BstMBI GATC 4 cut(s) 187, 445, 475, 767
BstMCI CGRYCG 3 cut(s) 9, 962, 1111
BstMWI GCNNNNNNNGC 6 cut(s) 41, 50, 166, 512, 853, 999
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 1 cut(s) 501
BstUI CGCG 1 cut(s) 1139
BstV1I GCAGC 3 cut(s) 386, 966, 1108
BstV2I GAAGAC 1 cut(s) 923
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
Bsu15I ATCGAT 1 cut(s) 1088
BsuRI GGCC 2 cut(s) 506, 1007
BsuTUI ATCGAT 1 cut(s) 1088
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 5 cut(s) 403, 522, 858, 864, 952
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 1 cut(s) 224
CfoI GCGC 3 cut(s) 217, 493, 1139
Cfr13I GGNCC 1 cut(s) 699
ClaI ATCGAT 1 cut(s) 1088
Csp6I GTAC 7 cut(s) 249, 271, 462, 715, 789, 1052, 1155
CviAII CATG 4 cut(s) 449, 543, 578, 1070
CviQI GTAC 7 cut(s) 249, 271, 462, 715, 789, 1052, 1155
DdeI CTNAG 2 cut(s) 117, 1021
DpnI GATC 4 cut(s) 189, 447, 477, 769
DpnII GATC 4 cut(s) 187, 445, 475, 767
DraI TTTAAA 1 cut(s) 91
DriI GACNNNNNGTC 2 cut(s) 112, 932
Eam1104I CTCTTC 4 cut(s) 383, 876, 983, 1241
Eam1105I GACNNNNNGTC 2 cut(s) 112, 932
EarI CTCTTC 4 cut(s) 383, 876, 983, 1241
Ecl136II GAGCTC 1 cut(s) 520
Eco130I CCWWGG 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 522
Eco31I GGTCTC 2 cut(s) 127, 492
Eco32I GATATC 1 cut(s) 966
Eco47I GGWCC 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 520
Eco88I CYCGRG 3 cut(s) 182, 239, 983
EcoICRI GAGCTC 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 582
EcoRV GATATC 1 cut(s) 966
EcoT14I CCWWGG 1 cut(s) 577
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 577
FaeI CATG 4 cut(s) 452, 546, 581, 1073
FaqI GGGAC 5 cut(s) 165, 168, 694, 1058, 1178
FatI CATG 4 cut(s) 448, 542, 577, 1069
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 4 cut(s) 375, 980, 1097, 1100
FokI GGATG 5 cut(s) 410, 509, 851, 865, 959
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 4 cut(s) 375, 980, 1097, 1100
FspBI CTAG 3 cut(s) 122, 311, 897
GlaI GCGC 3 cut(s) 216, 492, 1138
GluI GCNGC 4 cut(s) 375, 980, 1097, 1100
GsuI CTGGAG 1 cut(s) 452
HaeIII GGCC 2 cut(s) 506, 1007
HapII CCGG 2 cut(s) 503, 944
HhaI GCGC 3 cut(s) 217, 493, 1139
Hin1II CATG 4 cut(s) 452, 546, 581, 1073
Hin6I GCGC 3 cut(s) 215, 491, 1137
HinP1I GCGC 3 cut(s) 215, 491, 1137
HindIII AAGCTT 1 cut(s) 1256
HinfI GANTC 4 cut(s) 125, 142, 1090, 1190
HpaII CCGG 2 cut(s) 503, 944
HphI GGTGA 2 cut(s) 960, 1231
Hpy166II GTNNAC 3 cut(s) 358, 464, 574
Hpy188I TCNGA 3 cut(s) 175, 420, 876
Hpy188III TCNNGA 8 cut(s) 182, 197, 239, 311, 832, 850, 944, 1163
Hpy8I GTNNAC 3 cut(s) 358, 464, 574
Hpy99I CGWCG 4 cut(s) 880, 894, 930, 1037
HpyAV CCTTC 5 cut(s) 205, 373, 388, 679, 878
HpyCH4III ACNGT 2 cut(s) 367, 1159
HpyCH4IV ACGT 1 cut(s) 625
HpyCH4V TGCA 1 cut(s) 542
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 50, 166, 512, 853, 999
HpyF3I CTNAG 2 cut(s) 117, 1021
HpySE526I ACGT 1 cut(s) 625
Hsp92II CATG 4 cut(s) 452, 546, 581, 1073
HspAI GCGC 3 cut(s) 215, 491, 1137
Kpn2I TCCGGA 1 cut(s) 943
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 4 cut(s) 187, 445, 475, 767
LguI GCTCTTC 2 cut(s) 876, 983
LmnI GCTCC 2 cut(s) 241, 427
Lsp1109I GCAGC 3 cut(s) 386, 966, 1108
LweI GCATC 2 cut(s) 202, 843
MaeI CTAG 3 cut(s) 122, 311, 897
MaeII ACGT 1 cut(s) 625
MaeIII GTNAC 2 cut(s) 161, 229
MalI GATC 4 cut(s) 189, 447, 477, 769
MboI GATC 4 cut(s) 187, 445, 475, 767
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 7 cut(s) 306, 324, 582, 609, 709, 824, 1222
MlyI GAGTC 2 cut(s) 119, 1199
MmeI TCCRAC 2 cut(s) 726, 899
Mph1103I ATGCAT 1 cut(s) 544
MroI TCCGGA 1 cut(s) 943
MroXI GAANNNNTTC 1 cut(s) 885
MseI TTAA 5 cut(s) 90, 323, 608, 654, 827
MspA1I CMGCKG 1 cut(s) 731
MspCI CTTAAG 1 cut(s) 653
MspI CCGG 2 cut(s) 503, 944
MspR9I CCNGG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 960
MvnI CGCG 1 cut(s) 1139
MwoI GCNNNNNNNGC 6 cut(s) 41, 50, 166, 512, 853, 999
NciI CCSGG 1 cut(s) 503
NcoI CCATGG 1 cut(s) 577
NdeII GATC 4 cut(s) 187, 445, 475, 767
NlaIII CATG 4 cut(s) 452, 546, 581, 1073
NlaIV GGNNCC 1 cut(s) 189
NmeAIII GCCGAG 2 cut(s) 541, 983
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 1 cut(s) 546
PciSI GCTCTTC 2 cut(s) 876, 983
PctI GAATGC 1 cut(s) 960
PdmI GAANNNNTTC 1 cut(s) 885
PfeI GAWTC 2 cut(s) 142, 1090
PflFI GACNNNGTC 1 cut(s) 1070
PkrI GCNGC 4 cut(s) 376, 981, 1098, 1101
PleI GAGTC 2 cut(s) 119, 1198
PpsI GAGTC 2 cut(s) 119, 1198
Ppu21I YACGTR 1 cut(s) 626
PshBI ATTAAT 2 cut(s) 608, 827
PsiI TTATAA 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 522
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 1 cut(s) 699
PsuI RGATCY 1 cut(s) 187
PsyI GACNNNGTC 1 cut(s) 1070
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 7 cut(s) 250, 272, 463, 716, 790, 1053, 1156
RsaNI GTAC 7 cut(s) 249, 271, 462, 715, 789, 1052, 1155
SacI GAGCTC 1 cut(s) 522
SapI GCTCTTC 2 cut(s) 876, 983
SaqAI TTAA 5 cut(s) 90, 323, 608, 654, 827
SatI GCNGC 4 cut(s) 375, 980, 1097, 1100
Sau3AI GATC 4 cut(s) 187, 445, 475, 767
Sau96I GGNCC 1 cut(s) 699
SchI GAGTC 2 cut(s) 119, 1199
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 1 cut(s) 522
SfaNI GCATC 2 cut(s) 202, 843
SinI GGWCC 1 cut(s) 699
SmlI CTYRAG 2 cut(s) 293, 653
SmoI CTYRAG 2 cut(s) 293, 653
Sse9I AATT 7 cut(s) 306, 324, 582, 609, 709, 824, 1222
SsiI CCGC 5 cut(s) 5, 191, 673, 1100, 1111
SspMI CTAG 3 cut(s) 122, 311, 897
SstI GAGCTC 1 cut(s) 522
StyD4I CCNGG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 2 cut(s) 367, 1159
TaiI ACGT 1 cut(s) 628
TaqI TCGA 8 cut(s) 39, 196, 535, 766, 831, 889, 1065, 1088
TaqII GACCGA 4 cut(s) 151, 375, 687, 976
TasI AATT 7 cut(s) 306, 324, 582, 609, 709, 824, 1222
TatI WGTACW 2 cut(s) 270, 461
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 2 cut(s) 142, 1090
Tru1I TTAA 5 cut(s) 90, 323, 608, 654, 827
Tru9I TTAA 5 cut(s) 90, 323, 608, 654, 827
TscAI CASTG 1 cut(s) 224
TseI GCWGC 3 cut(s) 374, 979, 1096
TspDTI ATGAA 4 cut(s) 318, 714, 1006, 1059
TspGWI ACGGA 5 cut(s) 515, 893, 1050, 1142, 1185
TspRI CASTG 1 cut(s) 224
Tth111I GACNNNGTC 1 cut(s) 1070
Vha464I CTTAAG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 699
VspI ATTAAT 2 cut(s) 608, 827
XapI RAATTY 2 cut(s) 306, 582
XbaI TCTAGA 1 cut(s) 310
XceI RCATGY 1 cut(s) 546
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 1 cut(s) 885
XspI CTAG 3 cut(s) 122, 311, 897
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.