Rroxscaffold_2G00079650

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003966
Physical Location & Seq
Forward (+)
2622826 .. 2624616
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00079650.1

Sequence Viewer

Length: 648 bp
ATGCAGATTCCATTCGATTATCCAGACGAACTTTTAACTGCAGTTTATGGACACCATAGTCACGGTGCCGTCGACTCACTTACATTTAAGACCAACAGAAATACTTACGGACCCTTTGGTTGGACGTCTAAAGATCTCAGACGGATGAAACGCAACTCTTTCTCAATTCATGTGCCTGGAAGTAAGATTGTTGGCTTTCATGGCAGTTCAAAGAGTAAATGGTCGCCTGGTATTTGTTCAATAGGAGCCTACGTAAAGCCTTATCATCAACAAATTGAATACCCTCCATCAAAAGCTCTGACGAGTCCAAGCCGGCAGTGTAGGACCCAGCCCACCCACTTCCGGCCATACCACAAACCCTCCAAATGCCCCGTGCCCGGTTGGCCCAATATGGCGAAATTGCTAAGGGAACCCCAAGCACTCCGTCACGAGCTTTTCCAAGCCGCCGCCGCCACCCCCGTGGATCGCCACCCTAAAGGGATCGATTCCGAACGTAAACCATCGCCGCCTCCGGCCAAATTACCACCTCCACCATCGAAGACCCGATACTCCTCCGTCTTGAAGCAAAGGCAGCCCACTGCTCCAATACCAACATCGAAAGACAACGAGAAAGCGGAGCAGAAGACGAAGCCGGCCAGGCTCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

215

Amino Acids

24.1

Weight (kDa)

10.13

Isoelectric Point (pI)

54.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 3 - 84 9.2e-14 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 57
AatII GACGTC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 65
AccI GTMKAC 1 cut(s) 72
AciI CCGC 5 cut(s) 444, 447, 450, 506, 614
AclWI GGATC 2 cut(s) 471, 488
AcoI YGGCCR 3 cut(s) 344, 513, 633
AcyI GRCGYC 1 cut(s) 125
AfiI CCNNNNNNNGG 3 cut(s) 120, 342, 377
AgsI TTSAA 4 cut(s) 210, 240, 278, 562
AjnI CCWGG 3 cut(s) 175, 226, 635
AleI CACNNNNGTG 1 cut(s) 458
AluBI AGCT 2 cut(s) 296, 433
AluI AGCT 2 cut(s) 296, 433
AlwI GGATC 2 cut(s) 471, 488
AoxI GGCC 4 cut(s) 344, 383, 513, 633
ApeKI GCWGC 1 cut(s) 571
AspS9I GGNCC 3 cut(s) 110, 324, 384
AsuC2I CCSGG 1 cut(s) 378
AvaII GGWCC 2 cut(s) 110, 324
BaeGI GKGCMC 1 cut(s) 378
BanI GGYRCC 1 cut(s) 65
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 428
BbsI GAAGAC 2 cut(s) 545, 629
BbvI GCAGC 1 cut(s) 583
BccI CCATC 3 cut(s) 295, 508, 541
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 3 cut(s) 177, 228, 637
BcnI CCSGG 1 cut(s) 378
BfaI CTAG 1 cut(s) 646
BfmI CTRYAG 1 cut(s) 39
BglI GCCNNNNNGGC 2 cut(s) 382, 637
BglII AGATCT 1 cut(s) 133
BisI GCNGC 5 cut(s) 444, 447, 450, 506, 572
BlsI GCNGC 5 cut(s) 445, 448, 451, 507, 573
Bme1390I CCNGG 4 cut(s) 177, 228, 378, 637
Bme18I GGWCC 2 cut(s) 110, 324
BmgT120I GGNCC 3 cut(s) 110, 324, 384
BmiI GGNNCC 5 cut(s) 67, 112, 247, 326, 411
BmrFI CCNGG 4 cut(s) 177, 228, 378, 637
BpiI GAAGAC 2 cut(s) 545, 629
Bpu10I CCTNAGC 1 cut(s) 404
BpuMI CCSGG 1 cut(s) 378
Bsa29I ATCGAT 1 cut(s) 483
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 459
BsaXI ACNNNNNCTCC 4 cut(s) 237, 267, 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 342, 377
Bse118I RCCGGY 2 cut(s) 312, 631
BseBI CCWGG 3 cut(s) 177, 228, 637
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 1 cut(s) 150
BseLI CCNNNNNNNGG 3 cut(s) 120, 342, 377
BseMII CTCAG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 378
BseXI GCAGC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 327
BshFI GGCC 4 cut(s) 346, 385, 515, 635
BshNI GGYRCC 1 cut(s) 65
BshVI ATCGAT 1 cut(s) 483
BsiSI CCGG 5 cut(s) 313, 343, 378, 512, 632
BslI CCNNNNNNNGG 3 cut(s) 120, 342, 377
BsnI GGCC 4 cut(s) 346, 385, 515, 635
Bsp1286I GDGCHC 1 cut(s) 378
Bsp143I GATC 3 cut(s) 133, 463, 480
BspACI CCGC 5 cut(s) 444, 447, 450, 506, 614
BspANI GGCC 4 cut(s) 346, 385, 515, 635
BspCNI CTCAG 1 cut(s) 150
BspDI ATCGAT 1 cut(s) 483
BspLI GGNNCC 5 cut(s) 67, 112, 247, 326, 411
BspMAI CTGCAG 1 cut(s) 43
BspPI GGATC 2 cut(s) 471, 488
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 2 cut(s) 312, 631
BssAI RCCGGY 2 cut(s) 312, 631
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 3 cut(s) 133, 463, 480
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 428
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 3 cut(s) 177, 228, 637
Bst4CI ACNGT 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 314, 633, 642
BstDEI CTNAG 2 cut(s) 137, 404
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 1 cut(s) 150
BstKTI GATC 3 cut(s) 136, 466, 483
BstMBI GATC 3 cut(s) 133, 463, 480
BstMWI GCNNNNNNNGC 6 cut(s) 201, 382, 449, 571, 637, 641
BstNI CCWGG 3 cut(s) 177, 228, 637
BstSCI CCNGG 4 cut(s) 175, 226, 376, 635
BstSFI CTRYAG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 378
BstSNI TACGTA 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 2 cut(s) 545, 629
BstX2I RGATCY 1 cut(s) 133
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 1 cut(s) 133
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 4 cut(s) 346, 385, 515, 635
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 1 cut(s) 150
BtsI GCAGTG 2 cut(s) 323, 576
BtsIMutI CAGTG 2 cut(s) 323, 576
Cac8I GCNNGC 3 cut(s) 314, 633, 642
Cfr10I RCCGGY 2 cut(s) 312, 631
Cfr13I GGNCC 3 cut(s) 110, 324, 384
ClaI ATCGAT 1 cut(s) 483
CviAII CATG 2 cut(s) 170, 200
DdeI CTNAG 2 cut(s) 137, 404
DpnI GATC 3 cut(s) 135, 465, 482
DpnII GATC 3 cut(s) 133, 463, 480
DrdI GACNNNNNNGTC 1 cut(s) 57
DseDI GACNNNNNNGTC 1 cut(s) 57
EaeI YGGCCR 3 cut(s) 344, 513, 633
Eco105I TACGTA 1 cut(s) 253
Eco47I GGWCC 2 cut(s) 110, 324
EcoO109I RGGNCCY 1 cut(s) 324
EcoRII CCWGG 3 cut(s) 175, 226, 635
FaeI CATG 2 cut(s) 173, 203
FaiI YATR 6 cut(s) 48, 57, 171, 201, 349, 392
FatI CATG 2 cut(s) 169, 199
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 5 cut(s) 444, 447, 450, 506, 572
FokI GGATG 1 cut(s) 157
Fsp4HI GCNGC 5 cut(s) 444, 447, 450, 506, 572
FspBI CTAG 1 cut(s) 646
GluI GCNGC 5 cut(s) 444, 447, 450, 506, 572
GsaI CCCAGC 1 cut(s) 331
HaeIII GGCC 4 cut(s) 346, 385, 515, 635
HapII CCGG 5 cut(s) 313, 343, 378, 512, 632
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 2 cut(s) 173, 203
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 4 cut(s) 7, 74, 304, 485
HpaII CCGG 5 cut(s) 313, 343, 378, 512, 632
Hpy166II GTNNAC 2 cut(s) 73, 497
Hpy188I TCNGA 3 cut(s) 140, 300, 490
Hpy188III TCNNGA 3 cut(s) 23, 428, 559
Hpy8I GTNNAC 2 cut(s) 73, 497
Hpy99I CGWCG 1 cut(s) 74
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 3 cut(s) 125, 252, 493
HpyCH4V TGCA 2 cut(s) 4, 41
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 382, 449, 571, 637, 641
HpyF3I CTNAG 2 cut(s) 137, 404
HpySE526I ACGT 3 cut(s) 125, 252, 493
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 2 cut(s) 173, 203
KroI GCCGGC 2 cut(s) 312, 631
KroNI GCCGGC 2 cut(s) 314, 633
Kzo9I GATC 3 cut(s) 133, 463, 480
LmnI GCTCC 3 cut(s) 245, 586, 616
Lsp1109I GCAGC 1 cut(s) 583
MaeI CTAG 1 cut(s) 646
MaeII ACGT 3 cut(s) 125, 252, 493
MaeIII GTNAC 2 cut(s) 59, 425
MalI GATC 3 cut(s) 135, 465, 482
MboI GATC 3 cut(s) 133, 463, 480
MboII GAAGA 2 cut(s) 550, 634
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 378
MluCI AATT 4 cut(s) 165, 273, 398, 518
MlyI GAGTC 2 cut(s) 68, 313
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 5 cut(s) 294, 370, 519, 537, 562
MroNI GCCGGC 2 cut(s) 312, 631
MseI TTAA 2 cut(s) 35, 87
MslI CAYNNNNRTG 1 cut(s) 458
MspI CCGG 5 cut(s) 313, 343, 378, 512, 632
MspR9I CCNGG 4 cut(s) 177, 228, 378, 637
MvaI CCWGG 3 cut(s) 177, 228, 637
MwoI GCNNNNNNNGC 6 cut(s) 201, 382, 449, 571, 637, 641
NaeI GCCGGC 2 cut(s) 314, 633
NciI CCSGG 1 cut(s) 378
NdeII GATC 3 cut(s) 133, 463, 480
NgoMIV GCCGGC 2 cut(s) 312, 631
NlaIII CATG 2 cut(s) 173, 203
NlaIV GGNNCC 5 cut(s) 67, 112, 247, 326, 411
NmuCI GTSAC 2 cut(s) 59, 425
OliI CACNNNNGTG 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PdiI GCCGGC 2 cut(s) 314, 633
PfeI GAWTC 2 cut(s) 7, 485
PkrI GCNGC 5 cut(s) 445, 448, 451, 507, 573
PleI GAGTC 2 cut(s) 68, 312
PpsI GAGTC 2 cut(s) 68, 312
Ppu21I YACGTR 1 cut(s) 253
PpuMI RGGWCCY 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 324
Psp6I CCWGG 3 cut(s) 175, 226, 635
PspFI CCCAGC 1 cut(s) 327
PspGI CCWGG 3 cut(s) 175, 226, 635
PspN4I GGNNCC 5 cut(s) 67, 112, 247, 326, 411
PspPI GGNCC 3 cut(s) 110, 324, 384
PspPPI RGGWCCY 1 cut(s) 324
PstI CTGCAG 1 cut(s) 43
PsuI RGATCY 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 458
SalI GTCGAC 1 cut(s) 71
SaqAI TTAA 2 cut(s) 35, 87
SatI GCNGC 5 cut(s) 444, 447, 450, 506, 572
Sau3AI GATC 3 cut(s) 133, 463, 480
Sau96I GGNCC 3 cut(s) 110, 324, 384
SchI GAGTC 2 cut(s) 68, 313
ScrFI CCNGG 4 cut(s) 177, 228, 378, 637
SduI GDGCHC 1 cut(s) 378
SetI ASST 6 cut(s) 128, 255, 298, 435, 496, 529
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 2 cut(s) 110, 324
SmiMI CAYNNNNRTG 1 cut(s) 458
SnaBI TACGTA 1 cut(s) 253
Sse9I AATT 4 cut(s) 165, 273, 398, 518
SsiI CCGC 5 cut(s) 444, 447, 450, 506, 614
SspMI CTAG 1 cut(s) 646
StyD4I CCNGG 4 cut(s) 175, 226, 376, 635
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 3 cut(s) 128, 255, 496
TaqI TCGA 5 cut(s) 15, 72, 483, 536, 596
TasI AATT 4 cut(s) 165, 273, 398, 518
TauI GCSGC 4 cut(s) 446, 449, 452, 508
TfiI GAWTC 2 cut(s) 7, 485
Tru1I TTAA 2 cut(s) 35, 87
Tru9I TTAA 2 cut(s) 35, 87
TscAI CASTG 2 cut(s) 323, 583
TseFI GTSAC 2 cut(s) 59, 425
TseI GCWGC 1 cut(s) 571
Tsp45I GTSAC 2 cut(s) 59, 425
TspDTI ATGAA 3 cut(s) 158, 161, 188
TspGWI ACGGA 4 cut(s) 123, 157, 413, 544
TspRI CASTG 2 cut(s) 323, 583
VpaK11BI GGWCC 2 cut(s) 110, 324
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 1 cut(s) 646
ZraI GACGTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.