Rh4BG260700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003966
Physical Location & Seq
Forward (+)
43344127 .. 43348846
4720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG260700.1

Sequence Viewer

Length: 459 bp
ATGCAGATTAGGTTCAATTATCCAGACGAATTTTTGACTTCAGTTCATGGGTATTGTCAAGAAGACTGGAGCGGGTCCTTTGTCACCTCGCTTACTCTTAAGACCAACAGAAAAACGTACGGACCCTTTGGGTCGACTCAATATCGCAACCCGAGAGACTGTTTTACAATTCATGTGCCTGGAAATAAGATGGTTGGTTTTTATGGCAGAACAAACAAGTGGTTGGCTGCTGGTATTGCTTCAATAGGAGCATACCTAAAGCCTCATGATCAACAAATTGAATATCCTCCGCCAACAACACTGAAACAAGCAGGATATCCTTCGCAAACCAAGCTCAAGAAAGATGACAGACCCCAATACCAGAAACATTATACAATTCAAGATATTGGAGTTAACGGAAACCAGGGAAACCACAACGGAGCTTTCTATTTGGGTGACACGTATATCATCTATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jacalin

Protein Analysis

152

Amino Acids

17.37

Weight (kDa)

9.15

Isoelectric Point (pI)

26.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Jacalin PF01419 2 - 85 6.5e-16 Jacalin-like lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 134
AciI CCGC 2 cut(s) 72, 290
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 24
AfaI GTAC 1 cut(s) 119
AflII CTTAAG 1 cut(s) 98
AflIII ACRYGT 1 cut(s) 438
AgsI TTSAA 4 cut(s) 16, 243, 281, 380
AjnI CCWGG 2 cut(s) 178, 402
AluBI AGCT 2 cut(s) 334, 422
AluI AGCT 2 cut(s) 334, 422
Alw26I GTCTC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 227
ApoI RAATTY 1 cut(s) 29
AspS9I GGNCC 2 cut(s) 75, 122
AsuHPI GGTGA 2 cut(s) 76, 446
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 2 cut(s) 75, 122
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 180, 404
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 98
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
Bme1390I CCNGG 2 cut(s) 180, 404
Bme18I GGWCC 2 cut(s) 75, 122
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 75, 122
BmiI GGNNCC 2 cut(s) 76, 124
BmrFI CCNGG 2 cut(s) 180, 404
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 88
BpuEI CTTGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 71
BseBI CCWGG 2 cut(s) 180, 404
BseDI CCNNGG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 151
BsiWI CGTACG 1 cut(s) 117
BsmAI GTCTC 1 cut(s) 150
BsoBI CYCGRG 1 cut(s) 151
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 2 cut(s) 72, 290
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 76, 124
BspTI CTTAAG 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 72
BsrI ACTGG 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 403
BssMI GATC 1 cut(s) 268
Bst2UI CCWGG 2 cut(s) 180, 404
Bst4CI ACNGT 1 cut(s) 161
BstAFI CTTAAG 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 441
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 2 cut(s) 236, 331
BstNI CCWGG 2 cut(s) 180, 404
BstSCI CCNGG 2 cut(s) 178, 402
BstV1I GCAGC 1 cut(s) 214
BstV2I GAAGAC 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 265
Cfr13I GGNCC 2 cut(s) 75, 122
Csp6I GTAC 1 cut(s) 118
CviAII CATG 3 cut(s) 47, 173, 266
CviJI RGCY 4 cut(s) 227, 262, 334, 422
CviKI_1 RGCY 4 cut(s) 227, 262, 334, 422
CviQI GTAC 1 cut(s) 118
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
EciI GGCGGA 1 cut(s) 279
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 75, 122
Eco57I CTGAAG 1 cut(s) 24
Eco88I CYCGRG 1 cut(s) 151
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 178, 402
EcoRV GATATC 1 cut(s) 317
FaeI CATG 3 cut(s) 50, 176, 269
FaiI YATR 8 cut(s) 48, 174, 204, 253, 267, 372, 444, 453
FatI CATG 3 cut(s) 46, 172, 265
FauI CCCGC 1 cut(s) 65
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 134
Fnu4HI GCNGC 1 cut(s) 228
Fsp4HI GCNGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 88
Hin1II CATG 3 cut(s) 50, 176, 269
HincII GTYRAC 2 cut(s) 135, 394
HindII GTYRAC 2 cut(s) 135, 394
HinfI GANTC 1 cut(s) 136
HpaI GTTAAC 1 cut(s) 394
HphI GGTGA 2 cut(s) 76, 446
Hpy166II GTNNAC 2 cut(s) 135, 394
Hpy188III TCNNGA 5 cut(s) 23, 59, 266, 337, 380
Hpy8I GTNNAC 2 cut(s) 135, 394
HpyAV CCTTC 1 cut(s) 330
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 2 cut(s) 116, 440
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 236, 331
HpySE526I ACGT 2 cut(s) 116, 440
Hsp92II CATG 3 cut(s) 50, 176, 269
Ksp22I TGATCA 1 cut(s) 268
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 3 cut(s) 69, 248, 419
LpnPI CCDG 9 cut(s) 36, 52, 165, 192, 216, 297, 374, 389, 416
Lsp1109I GCAGC 1 cut(s) 214
MaeII ACGT 2 cut(s) 116, 440
MaeIII GTNAC 2 cut(s) 82, 434
MalI GATC 1 cut(s) 270
MbiI CCGCTC 1 cut(s) 72
MboI GATC 1 cut(s) 268
MboII GAAGA 1 cut(s) 74
MluCI AATT 5 cut(s) 16, 29, 168, 276, 375
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 3 cut(s) 97, 273, 297
MseI TTAA 2 cut(s) 99, 393
MspCI CTTAAG 1 cut(s) 98
MspR9I CCNGG 2 cut(s) 180, 404
MvaI CCWGG 2 cut(s) 180, 404
MwoI GCNNNNNNNGC 2 cut(s) 236, 331
NdeII GATC 1 cut(s) 268
NlaIII CATG 3 cut(s) 50, 176, 269
NlaIV GGNNCC 2 cut(s) 76, 124
NmuCI GTSAC 2 cut(s) 82, 434
PagI TCATGA 1 cut(s) 265
Pfl23II CGTACG 1 cut(s) 117
PkrI GCNGC 1 cut(s) 229
PleI GAGTC 1 cut(s) 130
PpsI GAGTC 1 cut(s) 130
Ppu21I YACGTR 1 cut(s) 441
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 178, 402
PspGI CCWGG 2 cut(s) 178, 402
PspLI CGTACG 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 76, 124
PspPI GGNCC 2 cut(s) 75, 122
PspPPI RGGWCCY 1 cut(s) 75
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SalI GTCGAC 1 cut(s) 133
SaqAI TTAA 2 cut(s) 99, 393
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 2 cut(s) 75, 122
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 2 cut(s) 180, 404
SetI ASST 7 cut(s) 14, 89, 119, 258, 336, 424, 443
SinI GGWCC 2 cut(s) 75, 122
SmlI CTYRAG 2 cut(s) 98, 335
SmoI CTYRAG 2 cut(s) 98, 335
Sse9I AATT 5 cut(s) 16, 29, 168, 276, 375
SsiI CCGC 2 cut(s) 72, 290
StyD4I CCNGG 2 cut(s) 178, 402
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 2 cut(s) 119, 443
TaqI TCGA 1 cut(s) 134
TasI AATT 5 cut(s) 16, 29, 168, 276, 375
Tru1I TTAA 2 cut(s) 99, 393
Tru9I TTAA 2 cut(s) 99, 393
TscAI CASTG 1 cut(s) 306
TseFI GTSAC 2 cut(s) 82, 434
TseI GCWGC 1 cut(s) 227
Tsp45I GTSAC 2 cut(s) 82, 434
TspDTI ATGAA 2 cut(s) 35, 161
TspGWI ACGGA 3 cut(s) 135, 411, 432
TspRI CASTG 1 cut(s) 306
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 75, 122
XapI RAATTY 1 cut(s) 29
XmiI GTMKAC 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.