Rorug02G0560700

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003966
Physical Location & Seq
Reverse (-)
68634966 .. 68637338
2373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0560700.1

Sequence Viewer

Length: 1137 bp
ATGTCCGAGACTCCTCACCTACCTAGCGAACCAGAGCCACCAAACCAACCCGACCCGCCCCAACTCACCCGCTGGTGCACCCATCACAACTTCAACCCCTTCGCCGATCCCATCGATTCCCGCAGCTCCGACGGCGACTTCTTCTCCGACGCCGCCTCCGATTCCGACCGTGACGTCAGCTGCTTCGTCACCGATCTCTTCGACCGTCGCGCCTCCTCCGATCATCATCATCCCCCCAGCGACTGCGTTCTCGTATCGGGTTCGGATCCGTTCGCGACCGAATTGGAGATCGAAGGTGACGACGGCGATGACCTCGGGTCAAATTCCAATTTGGGGCTAGGGTTTGGGGCCGAGTCAAATTCCCAGGGCTCAGGCTCTGAATTCGGTGACTTTCTAGCTCCGCAAGGGCTTCGGGTTGTTGGGTTGGAGTCCGATTCGGATTCGGATATCGATGAATTCGAAAGCAATCGGGTCAGCGAAGGTATCCAGTCGATTTGGGACAGTATTTTCATGGAGGAGCAGAGAAATGCGTATGAGAATTCTGAGTGGGAAGAAGTGGAGCAAAGAATTGAGCTGCCGGATAGTTCAATTGAGGGTGGAGGAGGAGGAGTGGAAGAGCTATCAGTGGCCTCTGGATTCTCTACTGAAGAAGAAGAGGTCCCCGAAGGAGCGGCGAGAAGCATTGAGTGGGAGATACTATTGACAGTGAACAATTTCAGGAGGAATGTGGATGATGTGGAGCTTGATATAGAGACTGCTGAGTACATCGAAGCGATTTTCGGGCAGTTTGTGGAGAATGCTAATGCCTGTAAAGGCAGCCCTCCGGCGGCTTTGCGGGCTGTGGAAGACCTTCCTTTTGTGGAGTTTTCGGTGGAGGAGCTGAGCAAGGAGGAGGTGGTTTGTGCTGTTTGTAAAGATGATATAGTGTTGGAGGATAAGGTGAGGAGCTTGCCGTGTTGTCATTATTACCATCAGGACTGCATCGTGCCGTGGTTGAGCATAAGGAATACATGCCCCGTGTGTCGGTATGAGTTGCCCACAGATGATCTTGATTATGAAAGGAGCAAGTGCGAGAGGGCTGCTCGTGGCCTGTCGTGGGAATCACAGGTTAGGCTTGATTTTGAACGGTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jacalin

Protein Analysis

378

Amino Acids

42.09

Weight (kDa)

4.1

Isoelectric Point (pI)

67.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 301 - 342 1.3e-11 Ring finger domain
zf-C3HC4_2 PF13923 301 - 341 2.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 301 - 328 6.5e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AatII GACGTC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 671
AccII CGCG 2 cut(s) 210, 275
AciI CCGC 8 cut(s) 56, 70, 121, 153, 401, 671, 827, 835
AclWI GGATC 3 cut(s) 101, 260, 273
AcsI RAATTY 5 cut(s) 322, 358, 380, 455, 538
AcuI CTGAAG 1 cut(s) 666
AcyI GRCGYC 2 cut(s) 150, 174
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 4 cut(s) 333, 826, 1023, 1096
AgsI TTSAA 3 cut(s) 94, 588, 1124
AhdI GACNNNNNGTC 1 cut(s) 316
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 8 cut(s) 126, 180, 398, 574, 619, 742, 880, 948
AluI AGCT 8 cut(s) 126, 180, 398, 574, 619, 742, 880, 948
Alw21I GWGCWC 1 cut(s) 80
Alw26I GTCTC 2 cut(s) 2, 746
Alw44I GTGCAC 1 cut(s) 76
AlwI GGATC 3 cut(s) 101, 260, 273
AlwNI CAGNNNCTG 2 cut(s) 243, 377
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 3 cut(s) 348, 627, 1087
ApaLI GTGCAC 1 cut(s) 76
ApeKI GCWGC 5 cut(s) 123, 180, 574, 816, 1079
ApoI RAATTY 5 cut(s) 322, 358, 380, 455, 538
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
Asp700I GAANNNNTTC 2 cut(s) 713, 849
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 348, 658
AsuHPI GGTGA 6 cut(s) 8, 58, 181, 308, 398, 952
AsuII TTCGAA 1 cut(s) 459
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 658
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 371
BauI CACGAG 1 cut(s) 1083
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 80
BbvI GCAGC 5 cut(s) 135, 167, 561, 828, 1066
BccI CCATC 3 cut(s) 90, 119, 978
BceAI ACGGC 4 cut(s) 148, 319, 937, 973
BcgI CGANNNNNNTGC 4 cut(s) 392, 426, 1061, 1095
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 1 cut(s) 494
BcoDI GTCTC 2 cut(s) 2, 746
BfaI CTAG 3 cut(s) 24, 338, 395
BfuI GTATCC 1 cut(s) 494
BisI GCNGC 8 cut(s) 124, 153, 181, 575, 672, 817, 828, 1080
BlpI GCTNAGC 1 cut(s) 881
BlsI GCNGC 8 cut(s) 125, 154, 182, 576, 673, 818, 829, 1081
Bme1390I CCNGG 1 cut(s) 365
Bme18I GGWCC 1 cut(s) 658
BmeRI GACNNNNNGTC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 348, 658
BmiI GGNNCC 3 cut(s) 267, 349, 660
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 1 cut(s) 990
BpiI GAAGAC 1 cut(s) 852
BplI GAGNNNNNCTC 2 cut(s) 1066, 1098
Bpu10I CCTNAGC 1 cut(s) 370
Bpu1102I GCTNAGC 1 cut(s) 881
Bpu14I TTCGAA 1 cut(s) 459
Bsa29I ATCGAT 2 cut(s) 114, 450
BsaBI GATNNNNATC 1 cut(s) 225
BsaHI GRCGYC 2 cut(s) 150, 174
BsaJI CCNNGG 4 cut(s) 313, 363, 364, 989
BsaXI ACNNNNNCTCC 6 cut(s) 128, 140, 158, 170, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 826, 1023, 1096
Bse1I ACTGG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 225
BseBI CCWGG 1 cut(s) 365
BseCI ATCGAT 2 cut(s) 114, 450
BseDI CCNNGG 4 cut(s) 313, 363, 364, 989
BseGI GGATG 2 cut(s) 229, 736
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 4 cut(s) 333, 826, 1023, 1096
BseMII CTCAG 4 cut(s) 384, 534, 750, 872
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 8 cut(s) 205, 530, 615, 618, 621, 890, 905, 958
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 5 cut(s) 135, 167, 561, 828, 1066
BseYI CCCAGC 1 cut(s) 236
Bsh1236I CGCG 2 cut(s) 210, 275
Bsh1285I CGRYCG 3 cut(s) 169, 205, 279
BshFI GGCC 3 cut(s) 350, 629, 1089
BshVI ATCGAT 2 cut(s) 114, 450
BsiEI CGRYCG 3 cut(s) 169, 205, 279
BsiHKAI GWGCWC 1 cut(s) 80
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 2 cut(s) 578, 824
BslFI GGGAC 2 cut(s) 512, 644
BslI CCNNNNNNNGG 4 cut(s) 333, 826, 1023, 1096
BsmAI GTCTC 2 cut(s) 2, 746
BsmFI GGGAC 2 cut(s) 512, 644
BsmI GAATGC 1 cut(s) 802
BsnI GGCC 3 cut(s) 350, 629, 1089
BsoBI CYCGRG 1 cut(s) 314
Bsp119I TTCGAA 1 cut(s) 459
Bsp1286I GDGCHC 2 cut(s) 80, 371
Bsp143I GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
Bsp1720I GCTNAGC 1 cut(s) 881
Bsp68I TCGCGA 1 cut(s) 275
BspACI CCGC 8 cut(s) 56, 70, 121, 153, 401, 671, 827, 835
BspANI GGCC 3 cut(s) 350, 629, 1089
BspCNI CTCAG 4 cut(s) 383, 535, 751, 873
BspDI ATCGAT 2 cut(s) 114, 450
BspFNI CGCG 2 cut(s) 210, 275
BspLI GGNNCC 3 cut(s) 267, 349, 660
BspPI GGATC 3 cut(s) 101, 260, 273
BspQI GCTCTTC 1 cut(s) 609
BspT104I TTCGAA 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 671
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 4 cut(s) 313, 363, 364, 989
BssMI GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
BssNI GRCGYC 2 cut(s) 150, 174
BssSI CACGAG 1 cut(s) 1083
Bst2BI CACGAG 1 cut(s) 1083
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 5 cut(s) 170, 206, 503, 706, 1128
Bst6I CTCTTC 3 cut(s) 203, 609, 648
BstACI GRCGYC 2 cut(s) 150, 174
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 837, 950
BstDEI CTNAG 4 cut(s) 370, 543, 759, 881
BstDSI CCRYGG 1 cut(s) 989
BstF5I GGATG 2 cut(s) 229, 736
BstFNI CGCG 2 cut(s) 210, 275
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 6 cut(s) 109, 196, 223, 268, 291, 1048
BstMAI GTCTC 2 cut(s) 2, 746
BstMBI GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
BstMCI CGRYCG 3 cut(s) 169, 205, 279
BstMWI GCNNNNNNNGC 2 cut(s) 132, 836
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 1014
BstSCI CCNGG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 80
BstUI CGCG 2 cut(s) 210, 275
BstV1I GCAGC 5 cut(s) 135, 167, 561, 828, 1066
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
Bsu15I ATCGAT 2 cut(s) 114, 450
BsuI GTATCC 1 cut(s) 494
BsuRI GGCC 3 cut(s) 350, 629, 1089
BsuTUI ATCGAT 2 cut(s) 114, 450
BtgI CCRYGG 1 cut(s) 989
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 2 cut(s) 229, 736
BtsIMutI CAGTG 2 cut(s) 630, 711
BtuMI TCGCGA 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 837, 950
CaiI CAGNNNCTG 2 cut(s) 243, 377
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 348, 658
ClaI ATCGAT 2 cut(s) 114, 450
CseI GACGC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 763
CviAII CATG 2 cut(s) 511, 1011
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 4 cut(s) 370, 543, 759, 881
DpnI GATC 6 cut(s) 108, 195, 222, 267, 290, 1047
DpnII GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
DrdI GACNNNNNNGTC 1 cut(s) 173
DriI GACNNNNNGTC 1 cut(s) 316
DseDI GACNNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 3 cut(s) 203, 609, 648
Eam1105I GACNNNNNGTC 1 cut(s) 316
EarI CTCTTC 3 cut(s) 203, 609, 648
Eco24I GRGCYC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 448
Eco47I GGWCC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 658
EcoRI GAATTC 3 cut(s) 380, 455, 538
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 371
FaeI CATG 2 cut(s) 514, 1014
FaiI YATR 8 cut(s) 512, 534, 749, 923, 1001, 1012, 1029, 1056
FaqI GGGAC 2 cut(s) 512, 644
FatI CATG 2 cut(s) 510, 1010
FauI CCCGC 4 cut(s) 63, 77, 128, 828
Fnu4HI GCNGC 8 cut(s) 124, 153, 181, 575, 672, 817, 828, 1080
FokI GGATG 2 cut(s) 216, 743
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 8 cut(s) 124, 153, 181, 575, 672, 817, 828, 1080
FspBI CTAG 3 cut(s) 24, 338, 395
GlaI GCGC 1 cut(s) 211
GluI GCNGC 8 cut(s) 124, 153, 181, 575, 672, 817, 828, 1080
GsaI CCCAGC 1 cut(s) 240
HaeIII GGCC 3 cut(s) 350, 629, 1089
HapII CCGG 2 cut(s) 578, 824
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 2 cut(s) 150, 174
Hin1II CATG 2 cut(s) 514, 1014
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HinfI GANTC 9 cut(s) 10, 116, 161, 353, 428, 434, 440, 636, 1100
HpaII CCGG 2 cut(s) 578, 824
HphI GGTGA 6 cut(s) 8, 58, 181, 308, 398, 952
Hpy166II GTNNAC 2 cut(s) 78, 709
Hpy188III TCNNGA 5 cut(s) 274, 633, 718, 974, 1049
Hpy8I GTNNAC 2 cut(s) 78, 709
Hpy99I CGWCG 4 cut(s) 134, 152, 210, 305
HpyAV CCTTC 5 cut(s) 109, 287, 473, 659, 860
HpyCH4III ACNGT 5 cut(s) 170, 206, 503, 706, 1128
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 2 cut(s) 78, 981
HpyF10VI GCNNNNNNNGC 2 cut(s) 132, 836
HpyF3I CTNAG 4 cut(s) 370, 543, 759, 881
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 2 cut(s) 150, 174
Hsp92II CATG 2 cut(s) 514, 1014
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 9 cut(s) 131, 403, 517, 559, 668, 739, 877, 945, 1062
Lsp1109I GCAGC 5 cut(s) 135, 167, 561, 828, 1066
LweI GCATC 1 cut(s) 990
MaeI CTAG 3 cut(s) 24, 338, 395
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 4 cut(s) 170, 187, 296, 386
MalI GATC 6 cut(s) 108, 195, 222, 267, 290, 1047
MbiI CCGCTC 1 cut(s) 671
MboI GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
MboII GAAGA 8 cut(s) 133, 190, 563, 626, 659, 662, 665, 857
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 80, 371
MlyI GAGTC 3 cut(s) 4, 362, 437
MmeI TCCRAC 5 cut(s) 153, 171, 189, 405, 909
MroXI GAANNNNTTC 2 cut(s) 713, 849
MseI TTAA 1 cut(s) 1135
MspA1I CMGCKG 2 cut(s) 72, 180
MspI CCGG 2 cut(s) 578, 824
MspR9I CCNGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 2 cut(s) 210, 275
MwoI GCNNNNNNNGC 2 cut(s) 132, 836
NdeII GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
NlaIII CATG 2 cut(s) 514, 1014
NlaIV GGNNCC 3 cut(s) 267, 349, 660
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 4 cut(s) 170, 187, 296, 386
NruI TCGCGA 1 cut(s) 275
NspI RCATGY 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 459
PasI CCCWGGG 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 609
PcsI WCGNNNNNNNCGW 3 cut(s) 156, 297, 456
PctI GAATGC 1 cut(s) 802
PdmI GAANNNNTTC 2 cut(s) 713, 849
PfeI GAWTC 6 cut(s) 116, 161, 434, 440, 636, 1100
PkrI GCNGC 8 cut(s) 125, 154, 182, 576, 673, 818, 829, 1081
PleI GAGTC 3 cut(s) 4, 361, 436
PpsI GAGTC 3 cut(s) 4, 361, 436
PpuMI RGGWCCY 1 cut(s) 658
Psp5II RGGWCCY 1 cut(s) 658
Psp6I CCWGG 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 236
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 3 cut(s) 267, 349, 660
PspPI GGNCC 2 cut(s) 348, 658
PspPPI RGGWCCY 1 cut(s) 658
PstNI CAGNNNCTG 2 cut(s) 243, 377
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 180
RruI TCGCGA 1 cut(s) 275
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
SapI GCTCTTC 1 cut(s) 609
SaqAI TTAA 1 cut(s) 1135
SatI GCNGC 8 cut(s) 124, 153, 181, 575, 672, 817, 828, 1080
Sau3AI GATC 6 cut(s) 106, 193, 220, 265, 288, 1045
Sau96I GGNCC 2 cut(s) 348, 658
SchI GAGTC 3 cut(s) 4, 362, 437
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 2 cut(s) 80, 371
SfaNI GCATC 1 cut(s) 990
SfuI TTCGAA 1 cut(s) 459
SinI GGWCC 1 cut(s) 658
SsiI CCGC 8 cut(s) 56, 70, 121, 153, 401, 671, 827, 835
SspMI CTAG 3 cut(s) 24, 338, 395
StyD4I CCNGG 1 cut(s) 363
TaaI ACNGT 5 cut(s) 170, 206, 503, 706, 1128
TaiI ACGT 1 cut(s) 177
TaqI TCGA 7 cut(s) 114, 201, 291, 450, 459, 491, 768
TaqII GACCGA 1 cut(s) 293
TatI WGTACW 1 cut(s) 762
TauI GCSGC 3 cut(s) 155, 674, 830
TfiI GAWTC 6 cut(s) 116, 161, 434, 440, 636, 1100
Tru1I TTAA 1 cut(s) 1135
Tru9I TTAA 1 cut(s) 1135
TscAI CASTG 2 cut(s) 630, 711
TseFI GTSAC 4 cut(s) 170, 187, 296, 386
TseI GCWGC 5 cut(s) 123, 180, 574, 816, 1079
Tsp45I GTSAC 4 cut(s) 170, 187, 296, 386
TspDTI ATGAA 3 cut(s) 468, 499, 1071
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 630, 711
VneI GTGCAC 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 658
XapI RAATTY 5 cut(s) 322, 358, 380, 455, 538
XceI RCATGY 1 cut(s) 1014
XmnI GAANNNNTTC 2 cut(s) 713, 849
XspI CTAG 3 cut(s) 24, 338, 395
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.