Rroxscaffold_5G00364950

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004253
Physical Location & Seq
Reverse (-)
46202696 .. 46236548
33853 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364950.1

Sequence Viewer

Length: 459 bp
ATGTTTTCCTTATTCTCGAGTCGAAAGGTTTTCCTCGTGTTTAGCCCACCAGCGAGCACAGAAACAACATTGTTTTCTCGGAGGGCACCACTGATCGAAAATGCAATTGTCCTCTGTTGTGCCAAAGGAAATAGTACGGGGCGTGACAATTATAGAGAAGCACTGGTCTGCAAGCTCCAAGAGCACATGATAGACCTGCTCCTGGATTCCACCTTGGGATGCGAACGTGTGTTAATGAAAGCAGCTCTTGCTTCGGTGGTTGGAGGCAAAGCCTTGGTAAATACGGACGGTGAACCGGTCATAGGAGACTTAGAGCCGGAAATGGACACTTTCACTACTTGTAGTCATAAGAACTACAGAGTAGTTGGCTGGTTCTCGAATGATGACGAACCAGGTCGGTCACCGATTTATTTTAGTTTAGCCGGCGAGGTAAGTATCTCGGAGCTCCAAATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

16.67

Weight (kDa)

5.01

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 85
AfaI GTAC 1 cut(s) 136
AfiI CCNNNNNNNGG 2 cut(s) 202, 302
AflIII ACRYGT 1 cut(s) 226
AgeI ACCGGT 1 cut(s) 295
AjnI CCWGG 2 cut(s) 201, 391
AluBI AGCT 3 cut(s) 175, 245, 445
AluI AGCT 3 cut(s) 175, 245, 445
Alw21I GWGCWC 3 cut(s) 59, 186, 447
Alw26I GTCTC 1 cut(s) 300
Ama87I CYCGRG 1 cut(s) 16
ApeKI GCWGC 1 cut(s) 242
AsiGI ACCGGT 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 302, 393
AvaI CYCGRG 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 88
BanI GGYRCC 1 cut(s) 85
BanII GRGCYC 1 cut(s) 447
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 3 cut(s) 59, 186, 447
BbvI GCAGC 1 cut(s) 254
BciT130I CCWGG 2 cut(s) 203, 393
BcoDI GTCTC 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 355
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 2 cut(s) 203, 393
BmeT110I CYCGRG 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 203, 393
BmsI GCATC 1 cut(s) 209
BsaJI CCNNGG 2 cut(s) 213, 273
BsaWI WCCGGW 1 cut(s) 295
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 302
Bse118I RCCGGY 2 cut(s) 295, 422
Bse1I ACTGG 1 cut(s) 168
BseBI CCWGG 2 cut(s) 203, 393
BseDI CCNNGG 2 cut(s) 213, 273
BseGI GGATG 1 cut(s) 224
BseLI CCNNNNNNNGG 2 cut(s) 202, 302
BseNI ACTGG 1 cut(s) 168
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 1 cut(s) 254
BshNI GGYRCC 1 cut(s) 85
BshTI ACCGGT 1 cut(s) 295
BsiHKAI GWGCWC 3 cut(s) 59, 186, 447
BsiHKCI CYCGRG 1 cut(s) 16
BsiSI CCGG 3 cut(s) 296, 317, 423
BslI CCNNNNNNNGG 2 cut(s) 202, 302
BsmAI GTCTC 1 cut(s) 300
BsoBI CYCGRG 1 cut(s) 16
Bsp1286I GDGCHC 4 cut(s) 59, 88, 186, 447
Bsp143I GATC 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 87
BspMI ACCTGC 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 85
BsrFI RCCGGY 2 cut(s) 295, 422
BsrI ACTGG 1 cut(s) 168
BssAI RCCGGY 2 cut(s) 295, 422
BssECI CCNNGG 2 cut(s) 213, 273
BssMI GATC 1 cut(s) 93
BssSI CACGAG 1 cut(s) 35
BssT1I CCWWGG 2 cut(s) 213, 273
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 203, 393
Bst4CI ACNGT 1 cut(s) 290
BstAPI GCANNNNNTGC 1 cut(s) 248
BstC8I GCNNGC 3 cut(s) 55, 173, 424
BstDEI CTNAG 1 cut(s) 310
BstEII GGTNACC 1 cut(s) 399
BstF5I GGATG 1 cut(s) 224
BstKTI GATC 1 cut(s) 96
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 181, 248
BstNI CCWGG 2 cut(s) 203, 393
BstPI GGTNACC 1 cut(s) 399
BstSCI CCNGG 2 cut(s) 201, 391
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 254
BtsCI GGATG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 89, 161
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 55, 173, 424
Cfr10I RCCGGY 2 cut(s) 295, 422
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 1 cut(s) 135
CspAI ACCGGT 1 cut(s) 295
CviAII CATG 1 cut(s) 187
CviJI RGCY 8 cut(s) 45, 175, 245, 272, 316, 369, 422, 445
CviKI_1 RGCY 8 cut(s) 45, 175, 245, 272, 316, 369, 422, 445
CviQI GTAC 1 cut(s) 135
DdeI CTNAG 1 cut(s) 310
DpnI GATC 1 cut(s) 95
DpnII GATC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 445
Eco130I CCWWGG 2 cut(s) 213, 273
Eco24I GRGCYC 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 445
Eco88I CYCGRG 1 cut(s) 16
Eco91I GGTNACC 1 cut(s) 399
EcoICRI GAGCTC 1 cut(s) 445
EcoO65I GGTNACC 1 cut(s) 399
EcoRII CCWGG 2 cut(s) 201, 391
EcoT14I CCWWGG 2 cut(s) 213, 273
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 213, 273
FaeI CATG 1 cut(s) 190
FaiI YATR 4 cut(s) 153, 188, 302, 348
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FatI CATG 1 cut(s) 186
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 231
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 243
HapII CCGG 3 cut(s) 296, 317, 423
Hin1II CATG 1 cut(s) 190
HinfI GANTC 2 cut(s) 19, 206
HpaII CCGG 3 cut(s) 296, 317, 423
HphI GGTGA 2 cut(s) 302, 393
Hpy166II GTNNAC 1 cut(s) 293
Hpy188I TCNGA 2 cut(s) 81, 442
Hpy188III TCNNGA 2 cut(s) 16, 376
Hpy8I GTNNAC 1 cut(s) 293
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 226
HpyCH4V TGCA 2 cut(s) 104, 171
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 248
HpyF3I CTNAG 1 cut(s) 310
HpySE526I ACGT 1 cut(s) 226
Hsp92II CATG 1 cut(s) 190
KroI GCCGGC 1 cut(s) 422
KroNI GCCGGC 1 cut(s) 424
Kzo9I GATC 1 cut(s) 93
LmnI GCTCC 4 cut(s) 180, 204, 442, 450
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 1 cut(s) 209
MabI ACCWGGT 1 cut(s) 391
MaeII ACGT 1 cut(s) 226
MaeIII GTNAC 2 cut(s) 143, 399
MalI GATC 1 cut(s) 95
MboI GATC 1 cut(s) 93
MfeI CAATTG 1 cut(s) 105
MhlI GDGCHC 4 cut(s) 59, 88, 186, 447
MluCI AATT 3 cut(s) 105, 148, 450
MlyI GAGTC 1 cut(s) 28
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 5 cut(s) 44, 75, 122, 257, 421
MroNI GCCGGC 1 cut(s) 422
MseI TTAA 1 cut(s) 233
MspI CCGG 3 cut(s) 296, 317, 423
MspR9I CCNGG 2 cut(s) 203, 393
MunI CAATTG 1 cut(s) 105
MvaI CCWGG 2 cut(s) 203, 393
MwoI GCNNNNNNNGC 2 cut(s) 181, 248
NaeI GCCGGC 1 cut(s) 424
NdeII GATC 1 cut(s) 93
NgoMIV GCCGGC 1 cut(s) 422
NlaIII CATG 1 cut(s) 190
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 143, 399
PaeR7I CTCGAG 1 cut(s) 16
PdiI GCCGGC 1 cut(s) 424
PfeI GAWTC 1 cut(s) 206
PfoI TCCNGGA 1 cut(s) 201
PinAI ACCGGT 1 cut(s) 295
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 201, 391
PspEI GGTNACC 1 cut(s) 399
PspGI CCWGG 2 cut(s) 201, 391
PspN4I GGNNCC 1 cut(s) 87
RsaI GTAC 1 cut(s) 136
RsaNI GTAC 1 cut(s) 135
SacI GAGCTC 1 cut(s) 447
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 1 cut(s) 93
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 203, 393
SduI GDGCHC 4 cut(s) 59, 88, 186, 447
SetI ASST 9 cut(s) 30, 177, 198, 215, 229, 247, 397, 432, 447
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 3 cut(s) 105, 148, 450
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 2 cut(s) 201, 391
StyI CCWWGG 2 cut(s) 213, 273
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 229
TaqI TCGA 4 cut(s) 17, 22, 96, 377
TaqII GACCGA 1 cut(s) 387
TasI AATT 3 cut(s) 105, 148, 450
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TscAI CASTG 2 cut(s) 96, 168
TseFI GTSAC 2 cut(s) 143, 399
TseI GCWGC 1 cut(s) 242
Tsp45I GTSAC 2 cut(s) 143, 399
TspDTI ATGAA 1 cut(s) 251
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 2 cut(s) 96, 168
XhoI CTCGAG 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.