Rorug05G0249500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004253
Physical Location & Seq
Forward (+)
27366104 .. 27367019
916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0249500.1

Sequence Viewer

Length: 681 bp
ATGGTGAGGACATGCTCGAAATGCGGGGACAGGAATCACAATTCAAGAACTTGCTGGAGCACCACGCAGTACTACCGGAGGCAGCAGCTGAATAAGCCGAAAGAAAGAAGAGCTAGTAATAGCAGTCTCAGGCTCTTTGGGGTTCAACTTAATCACCACGACCTTTCTTCTTCTTCTTCAAGAGTGGCATGGAGTGAGGAGGAACACCGGAAATTTCTTGAGGGGATTGAAAAGCTGGGTAGGGGTGATTGGAGGGGCATCTCTAAACACTATGTTACCACAAGATCTCCAACCCAGATCGCCAGTCATGCCCAAAAATATTTTCTCAGATGTCAAAATAGTACCACCGCCAACACTGCAACCATCAACAACCTCGACTTCAACTTTAACCCCCACAAGAAAAGACGCCGAGGTCGTCCCAGCCTTTTCGATGTCGGTAACGATCAACTTGCCTCACCCCGTCAACTGATTGATATTAACAGTAATAATCCCAATACTGAAGCATCTATTAACAATCCATATGAGTACTGCTGGCCAGCCGTTCATTATTCGACGCCCCTCCAAATTGATGTTAGTTTGAAGAACTCATCATCTCAGAGTGCAGCAGCAGCAGCAGGAGGAGGACTAGACTTAGAACTTAAGCTTGCAGCAAGCATAATGCCGTTGGGGTTAGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

226

Amino Acids

25.49

Weight (kDa)

9.68

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 61 - 108 7e-14 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 411
AciI CCGC 2 cut(s) 24, 348
AcoI YGGCCR 1 cut(s) 533
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 1 cut(s) 519
AcyI GRCGYC 2 cut(s) 406, 554
AfaI GTAC 3 cut(s) 71, 343, 527
AflII CTTAAG 1 cut(s) 638
AgsI TTSAA 6 cut(s) 45, 146, 180, 230, 382, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 306, 338
AluBI AGCT 4 cut(s) 88, 113, 235, 643
AluI AGCT 4 cut(s) 88, 113, 235, 643
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 131
AlwNI CAGNNNCTG 1 cut(s) 88
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 7 cut(s) 82, 85, 602, 605, 608, 611, 647
ApoI RAATTY 1 cut(s) 212
AsuHPI GGTGA 4 cut(s) 16, 146, 257, 447
BalI TGGCCA 1 cut(s) 535
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 7 cut(s) 94, 97, 614, 617, 620, 623, 659
BccI CCATC 1 cut(s) 371
BceAI ACGGC 2 cut(s) 524, 646
BcgI CGANNNNNNTGC 2 cut(s) 431, 465
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 2 cut(s) 114, 626
BfrI CTTAAG 1 cut(s) 638
BglII AGATCT 1 cut(s) 284
BisI GCNGC 7 cut(s) 83, 86, 603, 606, 609, 612, 648
BlsI GCNGC 7 cut(s) 84, 87, 604, 607, 610, 613, 649
BmcAI AGTACT 2 cut(s) 71, 527
BmsI GCATC 2 cut(s) 267, 512
BpmI CTGGAG 1 cut(s) 76
BpuEI CTTGAG 1 cut(s) 239
BsaHI GRCGYC 2 cut(s) 406, 554
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 2 cut(s) 75, 207
BsaXI ACNNNNNCTCC 2 cut(s) 271, 301
Bse1I ACTGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 409
BseMII CTCAG 3 cut(s) 142, 340, 608
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 2 cut(s) 212, 633
BseXI GCAGC 7 cut(s) 94, 97, 614, 617, 620, 623, 659
BseYI CCCAGC 2 cut(s) 235, 419
BsgI GTGCAG 1 cut(s) 621
BshFI GGCC 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 2 cut(s) 76, 208
BslFI GGGAC 2 cut(s) 41, 402
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 41, 402
BsnI GGCC 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 62
Bsp143I GATC 4 cut(s) 284, 297, 442, 673
BspACI CCGC 2 cut(s) 24, 348
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 3 cut(s) 141, 339, 607
BspQI GCTCTTC 1 cut(s) 103
BspTI CTTAAG 1 cut(s) 638
BsrI ACTGG 1 cut(s) 303
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 4 cut(s) 284, 297, 442, 673
BssNI GRCGYC 2 cut(s) 406, 554
Bst4CI ACNGT 1 cut(s) 482
Bst6I CTCTTC 1 cut(s) 103
BstACI GRCGYC 2 cut(s) 406, 554
BstAFI CTTAAG 1 cut(s) 638
BstC8I GCNNGC 4 cut(s) 533, 537, 645, 652
BstDEI CTNAG 4 cut(s) 128, 326, 594, 631
BstKTI GATC 4 cut(s) 287, 300, 445, 676
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 4 cut(s) 284, 297, 442, 673
BstMWI GCNNNNNNNGC 6 cut(s) 21, 94, 308, 356, 608, 611
BstNSI RCATGY 1 cut(s) 15
BstV1I GCAGC 7 cut(s) 94, 97, 614, 617, 620, 623, 659
BstX2I RGATCY 1 cut(s) 284
BstYI RGATCY 1 cut(s) 284
BsuRI GGCC 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 4 cut(s) 533, 537, 645, 652
CaiI CAGNNNCTG 1 cut(s) 88
CseI GACGC 2 cut(s) 414, 562
Csp6I GTAC 3 cut(s) 70, 342, 526
CviAII CATG 3 cut(s) 12, 189, 308
CviJI RGCY 9 cut(s) 88, 97, 113, 133, 235, 423, 535, 539, 643
CviKI_1 RGCY 9 cut(s) 88, 97, 113, 133, 235, 423, 535, 539, 643
CviQI GTAC 3 cut(s) 70, 342, 526
DdeI CTNAG 4 cut(s) 128, 326, 594, 631
DpnI GATC 4 cut(s) 286, 299, 444, 675
DpnII GATC 4 cut(s) 284, 297, 442, 673
DrdI GACNNNNNNGTC 1 cut(s) 411
DseDI GACNNNNNNGTC 1 cut(s) 411
EaeI YGGCCR 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 519
FaeI CATG 3 cut(s) 15, 192, 311
FaiI YATR 7 cut(s) 13, 190, 273, 309, 520, 522, 656
FaqI GGGAC 2 cut(s) 41, 402
FatI CATG 3 cut(s) 11, 188, 307
FauI CCCGC 1 cut(s) 17
FauNDI CATATG 1 cut(s) 520
Fnu4HI GCNGC 7 cut(s) 83, 86, 603, 606, 609, 612, 648
Fsp4HI GCNGC 7 cut(s) 83, 86, 603, 606, 609, 612, 648
FspBI CTAG 2 cut(s) 114, 626
GluI GCNGC 7 cut(s) 83, 86, 603, 606, 609, 612, 648
GsaI CCCAGC 2 cut(s) 239, 423
GsuI CTGGAG 1 cut(s) 76
HaeIII GGCC 1 cut(s) 535
HapII CCGG 2 cut(s) 76, 208
HgaI GACGC 2 cut(s) 414, 562
Hin1I GRCGYC 2 cut(s) 406, 554
Hin1II CATG 3 cut(s) 15, 192, 311
HincII GTYRAC 1 cut(s) 464
HindII GTYRAC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 1 cut(s) 34
HpaII CCGG 2 cut(s) 76, 208
HphI GGTGA 4 cut(s) 16, 146, 257, 447
Hpy166II GTNNAC 1 cut(s) 464
Hpy188I TCNGA 2 cut(s) 329, 597
Hpy188III TCNNGA 3 cut(s) 45, 180, 218
Hpy8I GTNNAC 1 cut(s) 464
Hpy99I CGWCG 1 cut(s) 556
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4V TGCA 3 cut(s) 359, 602, 647
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 94, 308, 356, 608, 611
HpyF3I CTNAG 4 cut(s) 128, 326, 594, 631
Hsp92I GRCGYC 2 cut(s) 406, 554
Hsp92II CATG 3 cut(s) 15, 192, 311
Kzo9I GATC 4 cut(s) 284, 297, 442, 673
LguI GCTCTTC 1 cut(s) 103
LmnI GCTCC 1 cut(s) 57
Lsp1109I GCAGC 7 cut(s) 94, 97, 614, 617, 620, 623, 659
LweI GCATC 2 cut(s) 267, 512
MaeI CTAG 2 cut(s) 114, 626
MaeIII GTNAC 2 cut(s) 274, 437
MalI GATC 4 cut(s) 286, 299, 444, 675
MboI GATC 4 cut(s) 284, 297, 442, 673
MboII GAAGA 6 cut(s) 120, 159, 162, 165, 168, 592
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 62
MlsI TGGCCA 1 cut(s) 535
MluCI AATT 3 cut(s) 40, 212, 564
MluNI TGGCCA 1 cut(s) 535
MmeI TCCRAC 1 cut(s) 314
Mox20I TGGCCA 1 cut(s) 535
MscI TGGCCA 1 cut(s) 535
MseI TTAA 5 cut(s) 150, 387, 477, 510, 639
Msp20I TGGCCA 1 cut(s) 535
MspA1I CMGCKG 1 cut(s) 88
MspCI CTTAAG 1 cut(s) 638
MspI CCGG 2 cut(s) 76, 208
MwoI GCNNNNNNNGC 6 cut(s) 21, 94, 308, 356, 608, 611
NdeI CATATG 1 cut(s) 520
NdeII GATC 4 cut(s) 284, 297, 442, 673
NlaIII CATG 3 cut(s) 15, 192, 311
NmeAIII GCCGAG 1 cut(s) 434
NspI RCATGY 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 103
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PfeI GAWTC 1 cut(s) 34
PkrI GCNGC 7 cut(s) 84, 87, 604, 607, 610, 613, 649
PspFI CCCAGC 2 cut(s) 235, 419
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 88
RsaI GTAC 3 cut(s) 71, 343, 527
RsaNI GTAC 3 cut(s) 70, 342, 526
SapI GCTCTTC 1 cut(s) 103
SaqAI TTAA 5 cut(s) 150, 387, 477, 510, 639
SatI GCNGC 7 cut(s) 83, 86, 603, 606, 609, 612, 648
Sau3AI GATC 4 cut(s) 284, 297, 442, 673
ScaI AGTACT 2 cut(s) 71, 527
SduI GDGCHC 1 cut(s) 62
SetI ASST 7 cut(s) 90, 115, 165, 237, 375, 415, 645
SfaNI GCATC 2 cut(s) 267, 512
SmlI CTYRAG 2 cut(s) 218, 638
SmoI CTYRAG 2 cut(s) 218, 638
Sse9I AATT 3 cut(s) 40, 212, 564
SsiI CCGC 2 cut(s) 24, 348
SspI AATATT 1 cut(s) 320
SspMI CTAG 2 cut(s) 114, 626
TaaI ACNGT 1 cut(s) 482
TaqI TCGA 4 cut(s) 17, 375, 429, 551
TasI AATT 3 cut(s) 40, 212, 564
TatI WGTACW 2 cut(s) 69, 525
TfiI GAWTC 1 cut(s) 34
Tru1I TTAA 5 cut(s) 150, 387, 477, 510, 639
Tru9I TTAA 5 cut(s) 150, 387, 477, 510, 639
TscAI CASTG 1 cut(s) 361
TseI GCWGC 7 cut(s) 82, 85, 602, 605, 608, 611, 647
TspDTI ATGAA 1 cut(s) 533
TspRI CASTG 1 cut(s) 361
Vha464I CTTAAG 1 cut(s) 638
XapI RAATTY 1 cut(s) 212
XceI RCATGY 1 cut(s) 15
XspI CTAG 2 cut(s) 114, 626
ZrmI AGTACT 2 cut(s) 71, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.