Rorug04G0049000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002107
Physical Location & Seq
Forward (+)
7621650 .. 7622403
754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0049000.1

Sequence Viewer

Length: 594 bp
ATGGCCGATGCGGTCTGCGTCAGCGACTGCTTTGAACACGTCAGGGTTGCCGGAGACGCCACCGAGGAGGAGGAGGAAGAAGATGACGACGAAGTAGGTGAAGGCGAGACCTGCCTCTGCAGGTCTGATTCGTCTCGGGTGTCTGATCGTTCGAGGCCGGCCACACCACGAAGAAGACCACACCCAAATCGGGTCTGGGATTGCTCACCATTTTCCTCGCCGCAGCAGCTCAACTCCAAATCCTTAGAGGTGTGCATCTCTGCCATTAACGCCTTCTATGATCAGGTTTTGAGTTATGGGAGACGCAGGTTTGTGATATTAGAAGCGCCAGCACACGATAACAATTGTGTTCTTGCTAATATTTTAGCCGCACATTTTCTCTGCTCATCTGATCCTTCTAAAGCTCCTTCACATCTTCAAGCGCCCAAGTCTCAACTCGAACAAGCTATCTCGTACGAGAATGCAGTTTTCAATCCTGTCAATTGTTTGATTTCCAAGGATAGGAATGATGAATTTGCCTTTGAGCTGCATTCTAAGGTGGACAAGTTGTTCTTAACTCGAATGAGTTTGATTCTGGTGTGGATAGTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

22.12

Weight (kDa)

4.95

Isoelectric Point (pI)

66.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 111, 119, 297
AciI CCGC 3 cut(s) 11, 221, 369
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 2 cut(s) 3, 159
AcsI RAATTY 1 cut(s) 512
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 190, 501
AflIII ACRYGT 1 cut(s) 37
AgsI TTSAA 3 cut(s) 35, 419, 472
AjiI CACGTC 1 cut(s) 40
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
AluBI AGCT 4 cut(s) 229, 404, 446, 526
AluI AGCT 4 cut(s) 229, 404, 446, 526
Alw26I GTCTC 5 cut(s) 48, 101, 138, 295, 435
AlwI GGATC 1 cut(s) 386
AlwNI CAGNNNCTG 1 cut(s) 27
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 3 cut(s) 3, 155, 159
ApeKI GCWGC 3 cut(s) 223, 226, 526
ApoI RAATTY 1 cut(s) 512
AspLEI GCGC 2 cut(s) 328, 424
AsuHPI GGTGA 2 cut(s) 110, 198
AvaI CYCGRG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 181
BbvI GCAGC 3 cut(s) 235, 238, 513
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 5 cut(s) 48, 101, 138, 295, 435
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 2 cut(s) 329, 425
BfuAI ACCTGC 3 cut(s) 111, 119, 297
BisI GCNGC 5 cut(s) 221, 224, 227, 369, 527
BlsI GCNGC 5 cut(s) 222, 225, 228, 370, 528
BmeT110I CYCGRG 1 cut(s) 135
BmgBI CACGTC 1 cut(s) 40
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 181
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 63, 495
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 501
Bse118I RCCGGY 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 63, 495
BseLI CCNNNNNNNGG 2 cut(s) 190, 501
BseRI GAGGAG 3 cut(s) 80, 83, 86
BseXI GCAGC 3 cut(s) 235, 238, 513
BshFI GGCC 3 cut(s) 5, 157, 161
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 2 cut(s) 51, 158
BsiWI CGTACG 1 cut(s) 453
BslI CCNNNNNNNGG 2 cut(s) 190, 501
BsmAI GTCTC 5 cut(s) 48, 101, 138, 295, 435
BsmBI CGTCTC 3 cut(s) 48, 138, 295
BsmI GAATGC 2 cut(s) 466, 529
BsnI GGCC 3 cut(s) 5, 157, 161
Bso31I GGTCTC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 135
Bsp143I GATC 3 cut(s) 145, 280, 391
BspACI CCGC 3 cut(s) 11, 221, 369
BspANI GGCC 3 cut(s) 5, 157, 161
BspMAI CTGCAG 1 cut(s) 122
BspMI ACCTGC 3 cut(s) 111, 119, 297
BspPI GGATC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 157
BssAI RCCGGY 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 63, 495
BssMI GATC 3 cut(s) 145, 280, 391
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 495
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 159, 330
BstDEI CTNAG 2 cut(s) 244, 534
BstH2I RGCGCY 2 cut(s) 329, 425
BstHHI GCGC 2 cut(s) 328, 424
BstKTI GATC 3 cut(s) 148, 283, 394
BstMAI GTCTC 5 cut(s) 48, 101, 138, 295, 435
BstMBI GATC 3 cut(s) 145, 280, 391
BstMWI GCNNNNNNNGC 4 cut(s) 56, 111, 226, 269
BstSFI CTRYAG 1 cut(s) 118
BstV1I GCAGC 3 cut(s) 235, 238, 513
BstV2I GAAGAC 1 cut(s) 181
BsuRI GGCC 3 cut(s) 5, 157, 161
BtrI CACGTC 1 cut(s) 40
BveI ACCTGC 3 cut(s) 111, 119, 297
Cac8I GCNNGC 2 cut(s) 159, 330
CaiI CAGNNNCTG 1 cut(s) 27
CfoI GCGC 2 cut(s) 328, 424
Cfr10I RCCGGY 1 cut(s) 157
CseI GACGC 3 cut(s) 7, 65, 312
Csp6I GTAC 1 cut(s) 454
CviJI RGCY 8 cut(s) 5, 157, 161, 229, 368, 404, 446, 526
CviKI_1 RGCY 8 cut(s) 5, 157, 161, 229, 368, 404, 446, 526
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 2 cut(s) 244, 534
DpnI GATC 3 cut(s) 147, 282, 393
DpnII GATC 3 cut(s) 145, 280, 391
EaeI YGGCCR 2 cut(s) 3, 159
Eco130I CCWWGG 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 101
Eco88I CYCGRG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 3 cut(s) 48, 138, 295
FaiI YATR 2 cut(s) 279, 297
FalI AAGNNNNNCTT 2 cut(s) 536, 568
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 221, 224, 227, 369, 527
FseI GGCCGGCC 1 cut(s) 161
Fsp4HI GCNGC 5 cut(s) 221, 224, 227, 369, 527
GlaI GCGC 2 cut(s) 327, 423
GluI GCNGC 5 cut(s) 221, 224, 227, 369, 527
HaeII RGCGCY 2 cut(s) 329, 425
HaeIII GGCC 3 cut(s) 5, 157, 161
HapII CCGG 2 cut(s) 51, 158
HgaI GACGC 3 cut(s) 7, 65, 312
HhaI GCGC 2 cut(s) 328, 424
Hin1I GRCGYC 1 cut(s) 57
Hin6I GCGC 2 cut(s) 326, 422
HinP1I GCGC 2 cut(s) 326, 422
HinfI GANTC 2 cut(s) 128, 571
HpaII CCGG 2 cut(s) 51, 158
HphI GGTGA 2 cut(s) 110, 198
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 3 cut(s) 127, 145, 391
Hpy8I GTNNAC 1 cut(s) 541
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 4 cut(s) 95, 283, 405, 417
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 4 cut(s) 120, 255, 464, 529
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 111, 226, 269
HpyF3I CTNAG 2 cut(s) 244, 534
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 57
HspAI GCGC 2 cut(s) 326, 422
KroI GCCGGC 1 cut(s) 157
KroNI GCCGGC 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 3 cut(s) 145, 280, 391
LmnI GCTCC 1 cut(s) 409
Lsp1109I GCAGC 3 cut(s) 235, 238, 513
LweI GCATC 1 cut(s) 264
MaeII ACGT 1 cut(s) 39
MalI GATC 3 cut(s) 147, 282, 393
MboI GATC 3 cut(s) 145, 280, 391
MboII GAAGA 5 cut(s) 89, 92, 183, 186, 407
MfeI CAATTG 2 cut(s) 343, 481
MluCI AATT 3 cut(s) 343, 481, 512
MnlI CCTC 8 cut(s) 58, 61, 64, 67, 125, 147, 226, 241
MroNI GCCGGC 1 cut(s) 157
MseI TTAA 2 cut(s) 267, 554
MspI CCGG 2 cut(s) 51, 158
MunI CAATTG 2 cut(s) 343, 481
Mva1269I GAATGC 2 cut(s) 466, 529
MwoI GCNNNNNNNGC 4 cut(s) 56, 111, 226, 269
NaeI GCCGGC 1 cut(s) 159
NdeII GATC 3 cut(s) 145, 280, 391
NgoMIV GCCGGC 1 cut(s) 157
PctI GAATGC 2 cut(s) 466, 529
PdiI GCCGGC 1 cut(s) 159
PfeI GAWTC 2 cut(s) 128, 571
Pfl23II CGTACG 1 cut(s) 453
PkrI GCNGC 5 cut(s) 222, 225, 228, 370, 528
PspLI CGTACG 1 cut(s) 453
PstI CTGCAG 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 27
RigI GGCCGGCC 1 cut(s) 161
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 267, 554
SatI GCNGC 5 cut(s) 221, 224, 227, 369, 527
Sau3AI GATC 3 cut(s) 145, 280, 391
SfaNI GCATC 1 cut(s) 264
SfcI CTRYAG 1 cut(s) 118
Sse9I AATT 3 cut(s) 343, 481, 512
SsiI CCGC 3 cut(s) 11, 221, 369
SspI AATATT 1 cut(s) 361
StyI CCWWGG 1 cut(s) 495
TaiI ACGT 1 cut(s) 42
TaqI TCGA 3 cut(s) 152, 438, 559
TasI AATT 3 cut(s) 343, 481, 512
TauI GCSGC 2 cut(s) 223, 371
TfiI GAWTC 2 cut(s) 128, 571
Tru1I TTAA 2 cut(s) 267, 554
Tru9I TTAA 2 cut(s) 267, 554
TseI GCWGC 3 cut(s) 223, 226, 526
TspDTI ATGAA 1 cut(s) 525
XapI RAATTY 1 cut(s) 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.