Rorug05G0152300

Auxin responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001295
Physical Location & Seq
Reverse (-)
14118043 .. 14119630
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0152300.1

Sequence Viewer

Length: 1191 bp
ATGGAGCGAAGCAATCCAAGTAGATGCGTGCAGCGAATGCGGGGCAGCAGGGGCTGCGGTGGTAGTAGTGGTGTAGGAGCAGCGAAGGCAAACTGCAGGGGCAGCAGCAGAGCTGGTCGCAGGGGCGCGCTAGCAGGCTGGAACGCAGAGCTGCAGGGAGGGGGCAGCAAGCAGCGATACAGGGGCGTGTGGCTGCGGGAGCAGCTCGCAGGGCAGCAGGGGCTGCTGTGCAAGGTTGCAGGCGGGGCAGCAGTAGGCGCAGGGGCGCACGCGGGGCAGGTTGCAGCGATGCTAGCACGGGCTAGGGGCTGCGGCAGTTTTTGGCGATATGAAATTCTTTTCGGGTTTTGGCTTTTTGGCTTTTGTGGTTTAGGGCTCCTAGGGGCTTTTTACCCGTTTTCAAGAGATCACTCAGACAAGAATTCAATCCGGCAAGAGCTCTATGTTTGGGAATCAGTGGCTGCATCAGCAAAGAAGGTGCTGGGGCTGCGATACCGGTTGCTTCCCTTGTTCTACACATCAATGTATCAAGCACACAAGAAGGGGACCCCAATTGCAAGGCCCGTATTCTTCTCATTTCCTGAAGATACCAATACATACGACATCAGTTCACAGTTTCTGATTGGTAAAGGCGTAATGGTGTCACCTGTGTTGCAGCAAGGAGCAAATTCAGTTGAGGCGTATTTTCCTGCTGGAAATTGGTTCGATCTCTTCAACTACACATCAGTCAGTGTTGATTCCGGAAAGAATGTTACGTTGGATGCACCGCCTGATCACATCAACGTGCATGTCCACGAAGGAAACATATTGGCCTTGCAAGGAGAGACCTTGACAACACAAGCAGCCCGAAACACTACATTCGAACTTTTGGTGGTCACTGGTAGTAGCAATGAGAGCACCGGAGAATTGTTCTTGGATGATGGAGAGGAAGTCGAAATGGGAGGAGAGGGAGGGAAGTGGAGTTTTGTGAGGTTTCGTTCTGCACGAGCACAAAATGGAAGTCTAGTTTTGAGCTCAAAGGTCGAAAATGGAGAATTTGCATTAAGCCAAGGTTGGATCATTGATAAAATTACCGTCCTTGGGTTGGACAAAGTTAATGCAACACAGTCCAGTGTATCCCTTCCTATAGGAAAGGAGTTTGAGTTGGAGCTGAAGCCGAATCAGACACTGGCGAAAACACTCTTTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

396

Amino Acids

42.81

Weight (kDa)

9.06

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_31_2nd PF01055 127 - 173 3.1e-12 Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd PF21365 182 - 270 3.1e-25 Glycosyl hydrolase family 31 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 268
AccII CGCG 2 cut(s) 128, 272
AccIII TCCGGA 1 cut(s) 740
AciI CCGC 7 cut(s) 40, 57, 196, 243, 272, 312, 767
AclWI GGATC 1 cut(s) 1064
AcsI RAATTY 4 cut(s) 333, 421, 667, 1034
AcuI CTGAAG 2 cut(s) 603, 1172
AfiI CCNNNNNNNGG 2 cut(s) 1080, 1084
AgeI ACCGGT 1 cut(s) 495
AgsI TTSAA 3 cut(s) 402, 426, 715
AjuI GAANNNNNNNTTGG 2 cut(s) 740, 772
AluBI AGCT 7 cut(s) 113, 151, 205, 439, 1014, 1150, 1188
AluI AGCT 7 cut(s) 113, 151, 205, 439, 1014, 1150, 1188
Alw21I GWGCWC 4 cut(s) 441, 899, 991, 1016
Alw26I GTCTC 1 cut(s) 818
AlwI GGATC 1 cut(s) 1064
AlwNI CAGNNNCTG 5 cut(s) 54, 223, 461, 619, 1168
Aor13HI TCCGGA 1 cut(s) 740
AoxI GGCC 2 cut(s) 560, 810
ApoI RAATTY 4 cut(s) 333, 421, 667, 1034
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
AsiGI ACCGGT 1 cut(s) 495
AspA2I CCTAGG 1 cut(s) 379
AspLEI GCGC 4 cut(s) 128, 130, 260, 268
AspS9I GGNCC 2 cut(s) 546, 561
AsuHPI GGTGA 1 cut(s) 636
AsuII TTCGAA 1 cut(s) 861
AsuNHI GCTAGC 2 cut(s) 130, 292
AvaII GGWCC 1 cut(s) 546
AvrII CCTAGG 1 cut(s) 379
BanII GRGCYC 3 cut(s) 378, 441, 1016
BauI CACGAG 1 cut(s) 984
Bbv12I GWGCWC 4 cut(s) 441, 899, 991, 1016
BccI CCATC 1 cut(s) 914
BciVI GTATCC 1 cut(s) 1126
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 1 cut(s) 818
BfaI CTAG 6 cut(s) 131, 293, 303, 380, 1004, 1189
BfmI CTRYAG 3 cut(s) 94, 152, 1125
BfuAI ACCTGC 1 cut(s) 268
BfuI GTATCC 1 cut(s) 1126
BlnI CCTAGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 546, 561
BmiI GGNNCC 3 cut(s) 377, 547, 548
BmsI GCATC 4 cut(s) 14, 279, 473, 751
BmtI GCTAGC 2 cut(s) 134, 296
Bpu14I TTCGAA 1 cut(s) 861
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 3 cut(s) 379, 1048, 1078
BsaWI WCCGGW 3 cut(s) 495, 740, 899
BsaXI ACNNNNNCTCC 4 cut(s) 915, 945, 1127, 1157
Bsc4I CCNNNNNNNGG 2 cut(s) 1080, 1084
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 3 cut(s) 883, 1110, 1173
Bse3DI GCAATG 1 cut(s) 895
BseAI TCCGGA 1 cut(s) 740
BseDI CCNNGG 3 cut(s) 379, 1048, 1078
BseGI GGATG 2 cut(s) 766, 922
BseLI CCNNNNNNNGG 2 cut(s) 1080, 1084
BseMI GCAATG 1 cut(s) 895
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 3 cut(s) 883, 1110, 1173
BsePI GCGCGC 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 957
BseYI CCCAGC 1 cut(s) 481
BsgI GTGCAG 2 cut(s) 50, 966
Bsh1236I CGCG 2 cut(s) 128, 272
BshFI GGCC 2 cut(s) 562, 812
BshTI ACCGGT 1 cut(s) 495
BsiHKAI GWGCWC 4 cut(s) 441, 899, 991, 1016
BsiSI CCGG 4 cut(s) 430, 496, 741, 900
BslFI GGGAC 1 cut(s) 559
BslI CCNNNNNNNGG 2 cut(s) 1080, 1084
BsmAI GTCTC 1 cut(s) 818
BsmFI GGGAC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 2 cut(s) 562, 812
Bso31I GGTCTC 1 cut(s) 818
Bsp119I TTCGAA 1 cut(s) 861
Bsp1286I GDGCHC 5 cut(s) 378, 441, 899, 991, 1016
Bsp13I TCCGGA 1 cut(s) 740
Bsp143I GATC 4 cut(s) 406, 706, 772, 1056
BspACI CCGC 7 cut(s) 40, 57, 196, 243, 272, 312, 767
BspANI GGCC 2 cut(s) 562, 812
BspCNI CTCAG 1 cut(s) 425
BspEI TCCGGA 1 cut(s) 740
BspFNI CGCG 2 cut(s) 128, 272
BspLI GGNNCC 3 cut(s) 377, 547, 548
BspMAI CTGCAG 2 cut(s) 98, 156
BspMI ACCTGC 1 cut(s) 268
BspOI GCTAGC 2 cut(s) 134, 296
BspPI GGATC 1 cut(s) 1064
BspT104I TTCGAA 1 cut(s) 861
BspTNI GGTCTC 1 cut(s) 818
BsrDI GCAATG 1 cut(s) 895
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 3 cut(s) 883, 1110, 1173
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 3 cut(s) 379, 1048, 1078
BssHII GCGCGC 1 cut(s) 126
BssMI GATC 4 cut(s) 406, 706, 772, 1056
BssSI CACGAG 1 cut(s) 984
BssT1I CCWWGG 3 cut(s) 379, 1048, 1078
Bst2BI CACGAG 1 cut(s) 984
Bst4CI ACNGT 3 cut(s) 615, 1075, 1107
Bst6I CTCTTC 1 cut(s) 716
BstAPI GCANNNNNTGC 3 cut(s) 37, 54, 223
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 9 cut(s) 29, 128, 132, 136, 170, 207, 241, 270, 294
BstDEI CTNAG 1 cut(s) 412
BstF5I GGATG 2 cut(s) 766, 922
BstFNI CGCG 2 cut(s) 128, 272
BstHHI GCGC 4 cut(s) 128, 130, 260, 268
BstKTI GATC 4 cut(s) 409, 709, 775, 1059
BstMAI GTCTC 1 cut(s) 818
BstMBI GATC 4 cut(s) 406, 706, 772, 1056
BstNSI RCATGY 1 cut(s) 791
BstSFI CTRYAG 3 cut(s) 94, 152, 1125
BstUI CGCG 2 cut(s) 128, 272
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 2 cut(s) 562, 812
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 2 cut(s) 766, 922
BtsIMutI CAGTG 5 cut(s) 462, 736, 876, 1117, 1166
BveI ACCTGC 1 cut(s) 268
Cac8I GCNNGC 9 cut(s) 29, 128, 132, 136, 170, 207, 241, 270, 294
CaiI CAGNNNCTG 5 cut(s) 54, 223, 461, 619, 1168
CfoI GCGC 4 cut(s) 128, 130, 260, 268
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 2 cut(s) 546, 561
CspAI ACCGGT 1 cut(s) 495
CviAII CATG 1 cut(s) 788
DdeI CTNAG 1 cut(s) 412
DpnI GATC 4 cut(s) 408, 708, 774, 1058
DpnII GATC 4 cut(s) 406, 706, 772, 1056
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
Ecl136II GAGCTC 2 cut(s) 439, 1014
Eco130I CCWWGG 3 cut(s) 379, 1048, 1078
Eco24I GRGCYC 3 cut(s) 378, 441, 1016
Eco31I GGTCTC 1 cut(s) 818
Eco47I GGWCC 1 cut(s) 546
Eco53kI GAGCTC 2 cut(s) 439, 1014
Eco57I CTGAAG 2 cut(s) 603, 1172
EcoICRI GAGCTC 2 cut(s) 439, 1014
EcoO109I RGGNCCY 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 421
EcoT14I CCWWGG 3 cut(s) 379, 1048, 1078
EcoT38I GRGCYC 3 cut(s) 378, 441, 1016
ErhI CCWWGG 3 cut(s) 379, 1048, 1078
FaeI CATG 1 cut(s) 791
FaiI YATR 6 cut(s) 330, 444, 598, 789, 806, 1127
FaqI GGGAC 1 cut(s) 559
FatI CATG 1 cut(s) 787
FauI CCCGC 4 cut(s) 33, 189, 236, 265
FbaI TGATCA 1 cut(s) 772
FokI GGATG 2 cut(s) 773, 929
FriOI GRGCYC 3 cut(s) 378, 441, 1016
FspBI CTAG 6 cut(s) 131, 293, 303, 380, 1004, 1189
GlaI GCGC 4 cut(s) 127, 129, 259, 267
GsaI CCCAGC 1 cut(s) 485
HaeIII GGCC 2 cut(s) 562, 812
HapII CCGG 4 cut(s) 430, 496, 741, 900
HhaI GCGC 4 cut(s) 128, 130, 260, 268
Hin1II CATG 1 cut(s) 791
Hin6I GCGC 4 cut(s) 126, 128, 258, 266
HinP1I GCGC 4 cut(s) 126, 128, 258, 266
HinfI GANTC 3 cut(s) 452, 737, 1159
HpaII CCGG 4 cut(s) 430, 496, 741, 900
HphI GGTGA 1 cut(s) 636
Hpy166II GTNNAC 2 cut(s) 611, 793
Hpy188I TCNGA 3 cut(s) 415, 621, 1164
Hpy188III TCNNGA 3 cut(s) 402, 581, 741
Hpy8I GTNNAC 2 cut(s) 611, 793
HpyAV CCTTC 5 cut(s) 79, 469, 535, 791, 1130
HpyCH4III ACNGT 3 cut(s) 615, 1075, 1107
HpyCH4IV ACGT 2 cut(s) 755, 783
HpyF3I CTNAG 1 cut(s) 412
HpySE526I ACGT 2 cut(s) 755, 783
Hsp92II CATG 1 cut(s) 791
HspAI GCGC 4 cut(s) 126, 128, 258, 266
KflI GGGWCCC 1 cut(s) 546
Kpn2I TCCGGA 1 cut(s) 740
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 4 cut(s) 406, 706, 772, 1056
LmnI GCTCC 6 cut(s) 4, 77, 199, 381, 662, 1147
LweI GCATC 4 cut(s) 14, 279, 473, 751
MaeI CTAG 6 cut(s) 131, 293, 303, 380, 1004, 1189
MaeII ACGT 2 cut(s) 755, 783
MaeIII GTNAC 3 cut(s) 642, 751, 874
MalI GATC 4 cut(s) 408, 708, 774, 1058
MboI GATC 4 cut(s) 406, 706, 772, 1056
MboII GAAGA 3 cut(s) 562, 596, 703
MfeI CAATTG 1 cut(s) 552
MhlI GDGCHC 5 cut(s) 378, 441, 899, 991, 1016
MluCI AATT 8 cut(s) 333, 421, 552, 667, 697, 905, 1034, 1068
MmeI TCCRAC 4 cut(s) 738, 1034, 1065, 1125
MnlI CCTC 7 cut(s) 152, 670, 919, 935, 940, 944, 963
MroI TCCGGA 1 cut(s) 740
MseI TTAA 2 cut(s) 1043, 1095
MslI CAYNNNNRTG 2 cut(s) 521, 782
MspI CCGG 4 cut(s) 430, 496, 741, 900
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 42
MvnI CGCG 2 cut(s) 128, 272
NdeII GATC 4 cut(s) 406, 706, 772, 1056
NheI GCTAGC 2 cut(s) 130, 292
NlaIII CATG 1 cut(s) 791
NlaIV GGNNCC 3 cut(s) 377, 547, 548
NmuCI GTSAC 2 cut(s) 642, 874
NspI RCATGY 1 cut(s) 791
NspV TTCGAA 1 cut(s) 861
PauI GCGCGC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 982
PctI GAATGC 1 cut(s) 42
PfeI GAWTC 3 cut(s) 452, 737, 1159
PinAI ACCGGT 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 546
Psp124BI GAGCTC 2 cut(s) 441, 1016
Psp5II RGGWCCY 1 cut(s) 546
PspFI CCCAGC 1 cut(s) 481
PspN4I GGNNCC 3 cut(s) 377, 547, 548
PspPI GGNCC 2 cut(s) 546, 561
PspPPI RGGWCCY 1 cut(s) 546
PstI CTGCAG 2 cut(s) 98, 156
PstNI CAGNNNCTG 5 cut(s) 54, 223, 461, 619, 1168
PteI GCGCGC 1 cut(s) 126
RseI CAYNNNNRTG 2 cut(s) 521, 782
SacI GAGCTC 2 cut(s) 441, 1016
SaqAI TTAA 2 cut(s) 1043, 1095
Sau3AI GATC 4 cut(s) 406, 706, 772, 1056
Sau96I GGNCC 2 cut(s) 546, 561
SduI GDGCHC 5 cut(s) 378, 441, 899, 991, 1016
SfaNI GCATC 4 cut(s) 14, 279, 473, 751
SfcI CTRYAG 3 cut(s) 94, 152, 1125
SfuI TTCGAA 1 cut(s) 861
SinI GGWCC 1 cut(s) 546
SmiMI CAYNNNNRTG 2 cut(s) 521, 782
Sse9I AATT 8 cut(s) 333, 421, 552, 667, 697, 905, 1034, 1068
SsiI CCGC 7 cut(s) 40, 57, 196, 243, 272, 312, 767
SspMI CTAG 6 cut(s) 131, 293, 303, 380, 1004, 1189
SstI GAGCTC 2 cut(s) 441, 1016
StyI CCWWGG 3 cut(s) 379, 1048, 1078
TaaI ACNGT 3 cut(s) 615, 1075, 1107
TaiI ACGT 2 cut(s) 758, 786
TaqI TCGA 4 cut(s) 705, 861, 933, 1023
TasI AATT 8 cut(s) 333, 421, 552, 667, 697, 905, 1034, 1068
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 3 cut(s) 452, 737, 1159
Tru1I TTAA 2 cut(s) 1043, 1095
Tru9I TTAA 2 cut(s) 1043, 1095
TscAI CASTG 5 cut(s) 462, 736, 883, 1117, 1173
TseFI GTSAC 2 cut(s) 642, 874
Tsp45I GTSAC 2 cut(s) 642, 874
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 5 cut(s) 462, 736, 883, 1117, 1173
VpaK11BI GGWCC 1 cut(s) 546
XapI RAATTY 4 cut(s) 333, 421, 667, 1034
XceI RCATGY 1 cut(s) 791
XmaJI CCTAGG 1 cut(s) 379
XspI CTAG 6 cut(s) 131, 293, 303, 380, 1004, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.