Rorug05G0184900

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002562
Physical Location & Seq
Reverse (-)
17910354 .. 17914186
3833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0184900.1

Sequence Viewer

Length: 540 bp
ATGAAGGAGATCAGTGGTAGAAAACAAGGTGGAATGTCTCCATGTGCAGCATGCAAGCTTCTGAGAAGGAGGTGTGCGCAGGATTGTGTGTTTGCTCCTTATTTTCCAGCTGATGAGCCTCACAAATTTGCAAGTGTGCACAAGGTTTTTGGTGCTAGCAATGTCAACAAGATGCTACAGGAATTACCAGAGCACCAGCGCAGTGATGCAGTGAGTTCGATGGTGTACGAGGCAAACGCTCGAGTAAGAGACCCTGTGTACGGCTGTGTTGGTGCAATCTCATCTCTACAGCAACAGATCGACGTCCTCCAAACTCAACTGGCCATTGCACAAGCTGAGGTGGTGCACATGAAAATGAATCAATTGCCTTCTGCATCACCTCCCGCAGCCACATCAGAAAACACCTCTCCCATCTCAGTCAAGCAAATAATGCCTAATAATAATAATTCTCACCATCATTATCATCCTCATCAGACCACCAAATCCTTTTTTACAATGGACATGGTGGTAGATCAGGCCAATTCTTTGTGGTCATGCTAG

Protein Analysis

179

Amino Acids

19.81

Weight (kDa)

8.24

Isoelectric Point (pI)

68.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LOB PF03195 14 - 111 1.4e-43 Lateral organ boundaries (LOB) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 306
Acc16I TGCGCA 1 cut(s) 78
AciI CCGC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 2 cut(s) 227, 260
AfiI CCNNNNNNNGG 1 cut(s) 260
AluBI AGCT 3 cut(s) 58, 110, 335
AluI AGCT 3 cut(s) 58, 110, 335
Alw21I GWGCWC 3 cut(s) 141, 195, 348
Alw26I GTCTC 2 cut(s) 42, 243
Alw44I GTGCAC 2 cut(s) 137, 344
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 2 cut(s) 321, 516
ApaLI GTGCAC 2 cut(s) 137, 344
ApeKI GCWGC 2 cut(s) 47, 386
ApoI RAATTY 1 cut(s) 125
AspLEI GCGC 2 cut(s) 79, 201
AsuHPI GGTGA 2 cut(s) 369, 443
AsuNHI GCTAGC 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 240
BaeGI GKGCMC 2 cut(s) 141, 348
BalI TGGCCA 1 cut(s) 323
Bbv12I GWGCWC 3 cut(s) 141, 195, 348
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 59, 398
BccI CCATC 3 cut(s) 214, 419, 462
BceAI ACGGC 1 cut(s) 277
BcgI CGANNNNNNTGC 2 cut(s) 198, 232
BcoDI GTCTC 2 cut(s) 42, 243
BfaI CTAG 2 cut(s) 156, 538
BfmI CTRYAG 2 cut(s) 176, 287
BisI GCNGC 2 cut(s) 48, 387
BlsI GCNGC 2 cut(s) 49, 388
BmeT110I CYCGRG 1 cut(s) 240
BmsI GCATC 3 cut(s) 162, 196, 383
BmtI GCTAGC 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 324
Bse3DI GCAATG 2 cut(s) 166, 324
BseGI GGATG 1 cut(s) 463
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 2 cut(s) 166, 324
BseMII CTCAG 3 cut(s) 53, 327, 429
BseNI ACTGG 1 cut(s) 324
BseSI GKGCMC 2 cut(s) 141, 348
BseXI GCAGC 2 cut(s) 59, 398
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 2 cut(s) 323, 518
BsiHKAI GWGCWC 3 cut(s) 141, 195, 348
BsiHKCI CYCGRG 1 cut(s) 240
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 2 cut(s) 42, 243
BsnI GGCC 2 cut(s) 323, 518
Bso31I GGTCTC 1 cut(s) 243
BsoBI CYCGRG 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 141, 195, 348
Bsp143I GATC 3 cut(s) 9, 297, 511
BspACI CCGC 1 cut(s) 384
BspANI GGCC 2 cut(s) 323, 518
BspCNI CTCAG 3 cut(s) 54, 328, 428
BspOI GCTAGC 1 cut(s) 159
BspTNI GGTCTC 1 cut(s) 243
BsrDI GCAATG 2 cut(s) 166, 324
BsrI ACTGG 1 cut(s) 324
BssMI GATC 3 cut(s) 9, 297, 511
BssNI GRCGYC 1 cut(s) 303
BstACI GRCGYC 1 cut(s) 303
BstAPI GCANNNNNTGC 1 cut(s) 430
BstC8I GCNNGC 3 cut(s) 52, 56, 157
BstDEI CTNAG 3 cut(s) 62, 336, 415
BstF5I GGATG 1 cut(s) 463
BstHHI GCGC 2 cut(s) 79, 201
BstKTI GATC 3 cut(s) 12, 300, 514
BstMAI GTCTC 2 cut(s) 42, 243
BstMBI GATC 3 cut(s) 9, 297, 511
BstMWI GCNNNNNNNGC 1 cut(s) 430
BstNSI RCATGY 1 cut(s) 54
BstSFI CTRYAG 2 cut(s) 176, 287
BstSLI GKGCMC 2 cut(s) 141, 348
BstV1I GCAGC 2 cut(s) 59, 398
BsuRI GGCC 2 cut(s) 323, 518
BtsCI GGATG 1 cut(s) 463
BtsI GCAGTG 2 cut(s) 208, 216
BtsIMutI CAGTG 3 cut(s) 19, 208, 216
Cac8I GCNNGC 3 cut(s) 52, 56, 157
CfoI GCGC 2 cut(s) 79, 201
Csp6I GTAC 2 cut(s) 226, 259
CviAII CATG 5 cut(s) 42, 51, 349, 502, 534
CviJI RGCY 8 cut(s) 58, 110, 118, 264, 323, 335, 389, 518
CviKI_1 RGCY 8 cut(s) 58, 110, 118, 264, 323, 335, 389, 518
CviQI GTAC 2 cut(s) 226, 259
DdeI CTNAG 3 cut(s) 62, 336, 415
DpnI GATC 3 cut(s) 11, 299, 513
DpnII GATC 3 cut(s) 9, 297, 511
EaeI YGGCCR 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 240
FaeI CATG 5 cut(s) 45, 54, 352, 505, 537
FaiI YATR 5 cut(s) 43, 52, 350, 503, 535
FatI CATG 5 cut(s) 41, 50, 348, 501, 533
FauI CCCGC 1 cut(s) 391
Fnu4HI GCNGC 2 cut(s) 48, 387
FokI GGATG 1 cut(s) 450
Fsp4HI GCNGC 2 cut(s) 48, 387
FspBI CTAG 2 cut(s) 156, 538
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 2 cut(s) 78, 200
GluI GCNGC 2 cut(s) 48, 387
HaeIII GGCC 2 cut(s) 323, 518
HhaI GCGC 2 cut(s) 79, 201
Hin1I GRCGYC 1 cut(s) 303
Hin1II CATG 5 cut(s) 45, 54, 352, 505, 537
Hin6I GCGC 2 cut(s) 77, 199
HinP1I GCGC 2 cut(s) 77, 199
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 1 cut(s) 358
HphI GGTGA 2 cut(s) 369, 443
Hpy166II GTNNAC 5 cut(s) 139, 166, 226, 259, 346
Hpy188I TCNGA 3 cut(s) 63, 397, 474
Hpy8I GTNNAC 5 cut(s) 139, 166, 226, 259, 346
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 2 cut(s) 60, 378
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 9 cut(s) 47, 54, 131, 139, 209, 275, 329, 346, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 430
HpyF3I CTNAG 3 cut(s) 62, 336, 415
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 1 cut(s) 303
Hsp92II CATG 5 cut(s) 45, 54, 352, 505, 537
HspAI GCGC 2 cut(s) 77, 199
Kzo9I GATC 3 cut(s) 9, 297, 511
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 8 cut(s) 65, 120, 164, 201, 209, 267, 305, 500
Lsp1109I GCAGC 2 cut(s) 59, 398
LweI GCATC 3 cut(s) 162, 196, 383
MaeI CTAG 2 cut(s) 156, 538
MaeII ACGT 1 cut(s) 303
MalI GATC 3 cut(s) 11, 299, 513
MboI GATC 3 cut(s) 9, 297, 511
MfeI CAATTG 1 cut(s) 362
MhlI GDGCHC 3 cut(s) 141, 195, 348
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 5 cut(s) 125, 182, 362, 445, 520
MluNI TGGCCA 1 cut(s) 323
MnlI CCTC 8 cut(s) 63, 129, 223, 317, 331, 390, 415, 477
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MslI CAYNNNNRTG 1 cut(s) 353
Msp20I TGGCCA 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 110
MunI CAATTG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 430
NdeII GATC 3 cut(s) 9, 297, 511
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 5 cut(s) 45, 54, 352, 505, 537
NsbI TGCGCA 1 cut(s) 78
NspI RCATGY 1 cut(s) 54
PaeI GCATGC 1 cut(s) 54
PaeR7I CTCGAG 1 cut(s) 240
PfeI GAWTC 1 cut(s) 358
PkrI GCNGC 2 cut(s) 49, 388
PspXI VCTCGAGB 1 cut(s) 240
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 227, 260
RsaNI GTAC 2 cut(s) 226, 259
RseI CAYNNNNRTG 1 cut(s) 353
SatI GCNGC 2 cut(s) 48, 387
Sau3AI GATC 3 cut(s) 9, 297, 511
SduI GDGCHC 3 cut(s) 141, 195, 348
SfaNI GCATC 3 cut(s) 162, 196, 383
SfcI CTRYAG 2 cut(s) 176, 287
Sfr274I CTCGAG 1 cut(s) 240
SlaI CTCGAG 1 cut(s) 240
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SphI GCATGC 1 cut(s) 54
Sse9I AATT 5 cut(s) 125, 182, 362, 445, 520
SsiI CCGC 1 cut(s) 384
SspMI CTAG 2 cut(s) 156, 538
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 218, 241, 300
TasI AATT 5 cut(s) 125, 182, 362, 445, 520
TfiI GAWTC 1 cut(s) 358
TscAI CASTG 3 cut(s) 19, 208, 216
TseI GCWGC 2 cut(s) 47, 386
TspDTI ATGAA 3 cut(s) 17, 365, 371
TspRI CASTG 3 cut(s) 19, 208, 216
VneI GTGCAC 2 cut(s) 137, 344
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 54
XhoI CTCGAG 1 cut(s) 240
XspI CTAG 2 cut(s) 156, 538
ZraI GACGTC 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.