Rh1BG053300

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0002562
Physical Location & Seq
Reverse (-)
7592355 .. 7595751
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG053300.1

Sequence Viewer

Length: 993 bp
ATGAAAAGTTTGCCACTGCAACATACATGTCGTCGCATAAGAAAGCTACGATGCTACGAGATTCTTTTAAGAAAATCTGATTTCGAATCGAAAAAGCCCCATTTTCTTCAACCCTCATCCTCTTCCACAACCTTCTATATCCTTTTCTCCATTCTTGTTCCAACTCACCAACTTGAGGAGAAGAAATCGCGACCTTGCAACGAGTCTCAGCGCAAACCCTATGGCATTTCACGCGAGGTCTATACCCTAATTGGCGAAAACGCCCTCCAACCAGTTGAGAAGGTTAGAGTTGTCAATGGCGTCCTGCCTGCTGGTGACTATTCCTTTGCTAAGAATAACAAGCACTGTCCGTCACGTCGTAAAGCTGTATACTTCTCTCATCTTATTGCTAGATGCTTTTATATTTCAATACATGATTATACATCAGGATACAGGTGCCAAGGTGGTTTGGCATTGGAGGGTCCTCAAGAGTCAAGACTCAAGAGGTTTAGTCTACCTGGCACGCTTCCACCTCAACTGGTGAAACTACATTACCTTAGAGAAATGAGGATTAGTGATATAAATGGAACAGAGCAGGATTTTCCTATGTTGAAAAACATGACTGGCCTAGTCAAATTGATACTGAGAAACTGTAACATTTCTGGAGAGATCCCTCCTTACCTATGGACACTGCAGAAACTGGAAATGCTGGATCTGAGTTTTAACAAGTTGATAGGAGAAATTCCAACAACAATCAGCTTAAAGCGTTTGAGATTCCTATTCTTAACTGGCAACTCTCTGAGTGGAAGTGTACCAGACTCAATCTTCATAGATGGAAGCAATGTGTATGTGTCGTCATTTTGGATGCTTCATATTATTCCTTTTTCTCTATTCATATATGCAATTTACTTTCTCATGTGTATATTATTACTCACTGGAATTCTAGGACATCATCACTTCAACCTTGTATGCAATTTGCGTTATGCTTGTAGAGAAGAAGCAAGTTGGTGCTAG

Protein Analysis

330

Amino Acids

38.04

Weight (kDa)

9.41

Isoelectric Point (pI)

52.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 202 - 241 8e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 435
AccI GTMKAC 2 cut(s) 369, 493
AccII CGCG 2 cut(s) 190, 234
AclWI GGATC 2 cut(s) 643, 699
AcsI RAATTY 2 cut(s) 720, 918
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 792
AfiI CCNNNNNNNGG 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 4 cut(s) 110, 408, 592, 940
AjiI CACGTC 1 cut(s) 356
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 3 cut(s) 46, 365, 738
AluI AGCT 3 cut(s) 46, 365, 738
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 2 cut(s) 643, 699
AlwNI CAGNNNCTG 1 cut(s) 679
AoxI GGCC 1 cut(s) 604
ApoI RAATTY 2 cut(s) 720, 918
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 3 cut(s) 158, 326, 532
AsuII TTCGAA 1 cut(s) 84
AvaII GGWCC 1 cut(s) 461
BanI GGYRCC 1 cut(s) 435
BccI CCATC 1 cut(s) 806
BciT130I CCWGG 1 cut(s) 498
BciVI GTATCC 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 4 cut(s) 390, 608, 923, 991
BfmI CTRYAG 1 cut(s) 671
BfuI GTATCC 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 498
Bme18I GGWCC 1 cut(s) 461
BmgBI CACGTC 1 cut(s) 356
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 2 cut(s) 437, 462
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 3 cut(s) 41, 383, 834
BpmI CTGGAG 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 84
BpuEI CTTGAG 3 cut(s) 194, 450, 464
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 439
Bsc4I CCNNNNNNNGG 1 cut(s) 175
Bse1I ACTGG 6 cut(s) 272, 522, 607, 684, 772, 919
Bse3DI GCAATG 1 cut(s) 826
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 2 cut(s) 116, 849
BseLI CCNNNNNNNGG 1 cut(s) 175
BseMI GCAATG 1 cut(s) 826
BseMII CTCAG 4 cut(s) 221, 614, 686, 770
BseNI ACTGG 6 cut(s) 272, 522, 607, 684, 772, 919
BseRI GAGGAG 1 cut(s) 191
Bsh1236I CGCG 2 cut(s) 190, 234
BshFI GGCC 1 cut(s) 606
BshNI GGYRCC 1 cut(s) 435
BslI CCNNNNNNNGG 1 cut(s) 175
BsmAI GTCTC 1 cut(s) 210
BsnI GGCC 1 cut(s) 606
Bsp119I TTCGAA 1 cut(s) 84
Bsp143I GATC 2 cut(s) 648, 691
Bsp68I TCGCGA 1 cut(s) 190
BspANI GGCC 1 cut(s) 606
BspCNI CTCAG 4 cut(s) 220, 615, 687, 771
BspFNI CGCG 2 cut(s) 190, 234
BspLI GGNNCC 2 cut(s) 437, 462
BspMAI CTGCAG 1 cut(s) 675
BspPI GGATC 2 cut(s) 643, 699
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 435
BsrDI GCAATG 1 cut(s) 826
BsrI ACTGG 6 cut(s) 272, 522, 607, 684, 772, 919
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 2 cut(s) 648, 691
BssNAI GTATAC 1 cut(s) 370
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 1 cut(s) 439
Bst1107I GTATAC 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 347, 632
Bst6I CTCTTC 1 cut(s) 127
BstACI GRCGYC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 2 cut(s) 309, 503
BstDEI CTNAG 6 cut(s) 207, 330, 536, 623, 695, 779
BstF5I GGATG 2 cut(s) 116, 849
BstFNI CGCG 2 cut(s) 190, 234
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 2 cut(s) 651, 694
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 2 cut(s) 648, 691
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 671
BstUI CGCG 2 cut(s) 190, 234
BstX2I RGATCY 2 cut(s) 648, 691
BstYI RGATCY 2 cut(s) 648, 691
BstZ17I GTATAC 1 cut(s) 370
BsuI GTATCC 1 cut(s) 422
BsuRI GGCC 1 cut(s) 606
BtrI CACGTC 1 cut(s) 356
BtsCI GGATG 2 cut(s) 116, 849
BtsI GCAGTG 2 cut(s) 14, 668
BtsIMutI CAGTG 4 cut(s) 14, 343, 668, 912
BtuMI TCGCGA 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 309, 503
CaiI CAGNNNCTG 1 cut(s) 679
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 461
CseI GACGC 1 cut(s) 289
Csp6I GTAC 1 cut(s) 791
CviAII CATG 4 cut(s) 27, 413, 598, 895
CviJI RGCY 5 cut(s) 46, 97, 365, 606, 738
CviKI_1 RGCY 5 cut(s) 46, 97, 365, 606, 738
CviQI GTAC 1 cut(s) 791
DdeI CTNAG 6 cut(s) 207, 330, 536, 623, 695, 779
DpnI GATC 2 cut(s) 650, 693
DpnII GATC 2 cut(s) 648, 691
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 461
EcoRI GAATTC 1 cut(s) 918
EcoRII CCWGG 1 cut(s) 496
EcoT14I CCWWGG 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 439
FaeI CATG 4 cut(s) 30, 416, 601, 898
FatI CATG 4 cut(s) 26, 412, 597, 894
FblI GTMKAC 2 cut(s) 369, 493
FokI GGATG 2 cut(s) 103, 856
FspBI CTAG 4 cut(s) 390, 608, 923, 991
GlaI GCGC 1 cut(s) 212
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 1 cut(s) 606
HgaI GACGC 1 cut(s) 289
HhaI GCGC 1 cut(s) 213
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 30, 416, 601, 898
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HinfI GANTC 7 cut(s) 61, 86, 203, 470, 477, 753, 797
HphI GGTGA 3 cut(s) 158, 326, 532
Hpy166II GTNNAC 3 cut(s) 370, 494, 791
Hpy188I TCNGA 3 cut(s) 79, 696, 780
Hpy188III TCNNGA 6 cut(s) 189, 426, 467, 474, 481, 642
Hpy8I GTNNAC 3 cut(s) 370, 494, 791
Hpy99I CGWCG 2 cut(s) 36, 360
HpyAV CCTTC 2 cut(s) 142, 274
HpyCH4III ACNGT 2 cut(s) 347, 632
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 5 cut(s) 19, 198, 673, 881, 951
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 6 cut(s) 207, 330, 536, 623, 695, 779
HpySE526I ACGT 1 cut(s) 355
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 30, 416, 601, 898
HspAI GCGC 1 cut(s) 211
Kzo9I GATC 2 cut(s) 648, 691
LweI GCATC 3 cut(s) 41, 383, 834
MaeI CTAG 4 cut(s) 390, 608, 923, 991
MaeII ACGT 1 cut(s) 355
MaeIII GTNAC 3 cut(s) 314, 351, 632
MalI GATC 2 cut(s) 650, 693
MboI GATC 2 cut(s) 648, 691
MboII GAAGA 5 cut(s) 98, 114, 193, 796, 986
MflI RGATCY 2 cut(s) 648, 691
MluCI AATT 6 cut(s) 249, 614, 720, 882, 918, 952
MlyI GAGTC 4 cut(s) 212, 471, 479, 791
MmeI TCCRAC 3 cut(s) 185, 292, 749
MseI TTAA 4 cut(s) 68, 702, 740, 764
MspR9I CCNGG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 2 cut(s) 190, 234
MwoI GCNNNNNNNGC 1 cut(s) 231
NdeII GATC 2 cut(s) 648, 691
NlaIII CATG 4 cut(s) 30, 416, 601, 898
NlaIV GGNNCC 2 cut(s) 437, 462
NmuCI GTSAC 2 cut(s) 314, 351
NruI TCGCGA 1 cut(s) 190
NspI RCATGY 1 cut(s) 30
NspV TTCGAA 1 cut(s) 84
PciI ACATGT 1 cut(s) 26
PfeI GAWTC 3 cut(s) 61, 86, 753
PleI GAGTC 4 cut(s) 211, 471, 478, 791
PpsI GAGTC 4 cut(s) 211, 471, 478, 791
PpuMI RGGWCCY 1 cut(s) 461
PscI ACATGT 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 2 cut(s) 437, 462
PspPI GGNCC 1 cut(s) 461
PspPPI RGGWCCY 1 cut(s) 461
PstI CTGCAG 1 cut(s) 675
PstNI CAGNNNCTG 1 cut(s) 679
PsuI RGATCY 2 cut(s) 648, 691
RruI TCGCGA 1 cut(s) 190
RsaI GTAC 1 cut(s) 792
RsaNI GTAC 1 cut(s) 791
SaqAI TTAA 4 cut(s) 68, 702, 740, 764
Sau3AI GATC 2 cut(s) 648, 691
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 4 cut(s) 212, 471, 479, 791
ScrFI CCNGG 1 cut(s) 498
SfaNI GCATC 3 cut(s) 41, 383, 834
SfcI CTRYAG 1 cut(s) 671
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 1 cut(s) 461
SmlI CTYRAG 3 cut(s) 173, 465, 479
SmoI CTYRAG 3 cut(s) 173, 465, 479
Sse9I AATT 6 cut(s) 249, 614, 720, 882, 918, 952
SspMI CTAG 4 cut(s) 390, 608, 923, 991
StyD4I CCNGG 1 cut(s) 496
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 2 cut(s) 347, 632
TaiI ACGT 1 cut(s) 358
TaqI TCGA 2 cut(s) 84, 89
TasI AATT 6 cut(s) 249, 614, 720, 882, 918, 952
TfiI GAWTC 3 cut(s) 61, 86, 753
Tru1I TTAA 4 cut(s) 68, 702, 740, 764
Tru9I TTAA 4 cut(s) 68, 702, 740, 764
TscAI CASTG 4 cut(s) 21, 350, 675, 919
TseFI GTSAC 2 cut(s) 314, 351
Tsp45I GTSAC 2 cut(s) 314, 351
TspDTI ATGAA 4 cut(s) 17, 796, 839, 862
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 4 cut(s) 21, 350, 675, 919
VpaK11BI GGWCC 1 cut(s) 461
XapI RAATTY 2 cut(s) 720, 918
XceI RCATGY 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 369, 493
XspI CTAG 4 cut(s) 390, 608, 923, 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.