Rorug06G0123300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002118
Physical Location & Seq
Forward (+)
17110539 .. 17113136
2598 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0123300.1

Sequence Viewer

Length: 570 bp
ATGGTTAATAATGAAACATTCCCAAAATTTCAATTTTCATTCCACAATAGCACATTATTTCTCTCTAGATCTTTCTCAGTAGCCGAAACCCGAAAGCTTGACGCCGAGACTGATTCACCAAACCCAAAAAAGGAGAATGGGGTCCAAGACGGAACGCCGAAGTTACAAGCAGTGGCTCAGAGGCGGTGGCTGGCCTTTTATCCTAAGGTAGATCCTTTCGATCTCAATGACTATAGATATGAGGGCCCTAGACTTCTGGGTTTACCCCCTCCTCAGGAATGCAATGCTACCGTCACAGTTACCTTTCTCACAGAGAGGCAAATTGCTAAGCTTCCGGATGCATCTACCTTGGACTCCTGTCAAGTTGTGGTTTTGGTTCTGGTGTCTGAGGCAGCTGCTGATGCACTTGACAACAATCAAACATCAACTATCCAGAATGCGGTAGATGGTGGTTTTGAATTGAATGTGCAGAATGATTATACTGCTCTTTGCAACATTTGCGTGGACTCAGGAGGATTTTGTGGGCAAAACACTACTAATGCTCAGTTCATTTGCAATTGCCCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WHIM1

Protein Analysis

189

Amino Acids

20.82

Weight (kDa)

4.56

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAK_assoc PF14380 105 - 187 8.3e-14 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 334
AciI CCGC 2 cut(s) 184, 440
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 26
AcyI GRCGYC 1 cut(s) 102
AfiI CCNNNNNNNGG 3 cut(s) 130, 274, 439
AgsI TTSAA 3 cut(s) 32, 458, 463
AluBI AGCT 3 cut(s) 97, 331, 395
AluI AGCT 3 cut(s) 97, 331, 395
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 398
Aor13HI TCCGGA 1 cut(s) 334
AoxI GGCC 2 cut(s) 192, 244
ApaI GGGCCC 1 cut(s) 248
ApeKI GCWGC 2 cut(s) 392, 395
ApoI RAATTY 1 cut(s) 26
AspS9I GGNCC 3 cut(s) 142, 244, 245
AsuHPI GGTGA 1 cut(s) 108
AvaII GGWCC 1 cut(s) 142
AxyI CCTNAGG 2 cut(s) 204, 273
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 248
BbvI GCAGC 2 cut(s) 382, 404
BccI CCATC 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 2 cut(s) 66, 249
BfmI CTRYAG 1 cut(s) 232
BglII AGATCT 1 cut(s) 68
BisI GCNGC 2 cut(s) 393, 396
BlpI GCTNAGC 1 cut(s) 327
BlsI GCNGC 2 cut(s) 394, 397
Bme18I GGWCC 1 cut(s) 142
BmgT120I GGNCC 3 cut(s) 142, 244, 245
BmiI GGNNCC 2 cut(s) 143, 246
BmsI GCATC 3 cut(s) 328, 350, 391
BoxI GACNNNNGTC 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 348
BsaWI WCCGGW 1 cut(s) 334
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 274, 439
Bse21I CCTNAGG 2 cut(s) 204, 273
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 348
BseGI GGATG 1 cut(s) 343
BseLI CCNNNNNNNGG 3 cut(s) 130, 274, 439
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 6 cut(s) 90, 191, 287, 378, 522, 557
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 2 cut(s) 382, 404
BsgI GTGCAG 1 cut(s) 488
BshFI GGCC 2 cut(s) 194, 246
BsiSI CCGG 1 cut(s) 335
BslI CCNNNNNNNGG 3 cut(s) 130, 274, 439
BsmAI GTCTC 1 cut(s) 101
BsmI GAATGC 2 cut(s) 284, 442
BsnI GGCC 2 cut(s) 194, 246
Bsp120I GGGCCC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 248
Bsp13I TCCGGA 1 cut(s) 334
Bsp143I GATC 3 cut(s) 68, 211, 220
Bsp1720I GCTNAGC 1 cut(s) 327
BspACI CCGC 2 cut(s) 184, 440
BspANI GGCC 2 cut(s) 194, 246
BspCNI CTCAG 6 cut(s) 89, 190, 286, 379, 521, 556
BspEI TCCGGA 1 cut(s) 334
BspLI GGNNCC 2 cut(s) 143, 246
BspPI GGATC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 289
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 3 cut(s) 68, 211, 220
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 348
Bst4CI ACNGT 2 cut(s) 292, 298
BstACI GRCGYC 1 cut(s) 102
BstAPI GCANNNNNTGC 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 8 cut(s) 76, 177, 204, 273, 327, 387, 508, 543
BstF5I GGATG 1 cut(s) 343
BstKTI GATC 3 cut(s) 71, 214, 223
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 3 cut(s) 68, 211, 220
BstMWI GCNNNNNNNGC 2 cut(s) 401, 498
BstPAI GACNNNNGTC 1 cut(s) 357
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 2 cut(s) 382, 404
BstX2I RGATCY 2 cut(s) 68, 211
BstYI RGATCY 2 cut(s) 68, 211
Bsu36I CCTNAGG 2 cut(s) 204, 273
BsuRI GGCC 2 cut(s) 194, 246
BtsCI GGATG 1 cut(s) 343
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 192
CaiI CAGNNNCTG 1 cut(s) 398
Cfr13I GGNCC 3 cut(s) 142, 244, 245
CseI GACGC 1 cut(s) 110
CviJI RGCY 8 cut(s) 83, 97, 176, 190, 194, 246, 331, 395
CviKI_1 RGCY 8 cut(s) 83, 97, 176, 190, 194, 246, 331, 395
DdeI CTNAG 8 cut(s) 76, 177, 204, 273, 327, 387, 508, 543
DpnI GATC 3 cut(s) 70, 213, 222
DpnII GATC 3 cut(s) 68, 211, 220
Eco130I CCWWGG 1 cut(s) 348
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 142
Eco81I CCTNAGG 2 cut(s) 204, 273
EcoO109I RGGNCCY 2 cut(s) 244, 245
EcoT14I CCWWGG 1 cut(s) 348
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 348
FaiI YATR 3 cut(s) 234, 240, 480
Fnu4HI GCNGC 2 cut(s) 393, 396
FokI GGATG 1 cut(s) 350
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 2 cut(s) 393, 396
FspBI CTAG 2 cut(s) 66, 249
GluI GCNGC 2 cut(s) 393, 396
HaeIII GGCC 2 cut(s) 194, 246
HapII CCGG 1 cut(s) 335
HgaI GACGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 102
HindIII AAGCTT 2 cut(s) 95, 329
HinfI GANTC 3 cut(s) 113, 353, 506
HpaII CCGG 1 cut(s) 335
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 2 cut(s) 263, 505
Hpy188I TCNGA 2 cut(s) 180, 388
Hpy188III TCNNGA 5 cut(s) 66, 275, 335, 433, 510
Hpy8I GTNNAC 2 cut(s) 263, 505
HpyCH4III ACNGT 2 cut(s) 292, 298
HpyCH4V TGCA 6 cut(s) 282, 341, 404, 469, 492, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 498
HpyF3I CTNAG 8 cut(s) 76, 177, 204, 273, 327, 387, 508, 543
Hsp92I GRCGYC 1 cut(s) 102
Kpn2I TCCGGA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 68, 211, 220
LpnPI CCDG 8 cut(s) 176, 242, 260, 348, 365, 370, 446, 495
Lsp1109I GCAGC 2 cut(s) 382, 404
LweI GCATC 3 cut(s) 328, 350, 391
MaeI CTAG 2 cut(s) 66, 249
MaeIII GTNAC 3 cut(s) 162, 292, 298
MalI GATC 3 cut(s) 70, 213, 222
MboI GATC 3 cut(s) 68, 211, 220
MfeI CAATTG 1 cut(s) 556
MflI RGATCY 2 cut(s) 68, 211
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 6 cut(s) 26, 32, 321, 458, 556, 564
MlyI GAGTC 2 cut(s) 347, 500
MnlI CCTC 7 cut(s) 174, 235, 279, 282, 309, 382, 506
Mph1103I ATGCAT 1 cut(s) 343
MroI TCCGGA 1 cut(s) 334
MseI TTAA 2 cut(s) 6, 568
MslI CAYNNNNRTG 1 cut(s) 500
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 1 cut(s) 335
MunI CAATTG 1 cut(s) 556
Mva1269I GAATGC 2 cut(s) 284, 442
MwoI GCNNNNNNNGC 2 cut(s) 401, 498
NdeII GATC 3 cut(s) 68, 211, 220
NlaIV GGNNCC 2 cut(s) 143, 246
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 343
PctI GAATGC 2 cut(s) 284, 442
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 2 cut(s) 394, 397
PleI GAGTC 2 cut(s) 347, 500
PpsI GAGTC 2 cut(s) 347, 500
PshAI GACNNNNGTC 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 143, 246
PspOMI GGGCCC 1 cut(s) 244
PspPI GGNCC 3 cut(s) 142, 244, 245
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 2 cut(s) 68, 211
PvuII CAGCTG 1 cut(s) 395
RseI CAYNNNNRTG 1 cut(s) 500
SaqAI TTAA 2 cut(s) 6, 568
SatI GCNGC 2 cut(s) 393, 396
Sau3AI GATC 3 cut(s) 68, 211, 220
Sau96I GGNCC 3 cut(s) 142, 244, 245
SchI GAGTC 2 cut(s) 347, 500
SduI GDGCHC 1 cut(s) 248
SetI ASST 6 cut(s) 99, 210, 305, 333, 350, 397
SfaNI GCATC 3 cut(s) 328, 350, 391
SfcI CTRYAG 1 cut(s) 232
SinI GGWCC 1 cut(s) 142
SmiMI CAYNNNNRTG 1 cut(s) 500
Sse9I AATT 6 cut(s) 26, 32, 321, 458, 556, 564
SsiI CCGC 2 cut(s) 184, 440
SspMI CTAG 2 cut(s) 66, 249
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 2 cut(s) 292, 298
TaqI TCGA 1 cut(s) 219
TasI AATT 6 cut(s) 26, 32, 321, 458, 556, 564
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 2 cut(s) 6, 568
Tru9I TTAA 2 cut(s) 6, 568
TscAI CASTG 1 cut(s) 177
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 2 cut(s) 392, 395
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 3 cut(s) 27, 27, 538
TspGWI ACGGA 1 cut(s) 165
TspRI CASTG 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 142
XapI RAATTY 1 cut(s) 26
XbaI TCTAGA 1 cut(s) 65
XspI CTAG 2 cut(s) 66, 249
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.