Rh2CG098900

Flavonoid 3-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000606
Physical Location & Seq
Reverse (-)
8209405 .. 8211489
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG098900.1

Sequence Viewer

Length: 822 bp
ATGAAATCCGGCCAAGAGATAAAATGGAAAGCAAAAATAGCAAAGCAAATTTTAAGGACGCATGATCATGTGTTTGCCTCCAGACCCCAAGCTGCAGCTGGCAAGTACACCACTTACAACCACCTCAACATCAGTTGGGCACCTCACGGCCCATATTGGCGCCAAGGCCGCAAAATCTACCTCTCCGAGCTATTCAACTCGAAGAGACTAGACTCTTTTGAGTACATTCGTGTGGAGGAAACTTGCGCTTTCCTCTCGCAGCTATGTGCCTTGTCAGGCAAGCCAGTCACCCTCAAGGAGCACCTCTCACGCCTCACTCTCGGCATTATAAGTAGGATTGTGTTGGGGAAGAAGTACTTCAGCGTGTCTGAGTCTGAGACTTCGATTGTGGCACTCGACGAGTTCCAGCAGATGCTGGACGAGTTGTTCTTGCTTAATGGGGTGTTGAACATAGGGGATTGGATACCCTGGCTCGAGCTTTTGGACTTGCAAGGTTATGTTAAGCGTATGAAGGCTTTAAAGAAAAAGTTCGACAGATTTCATGACCATGTTTTCGGTGAACACAGATCAAACAAGGAAGGTGTAAAGGAGTTTGTGCCAAAGGACATAGTAGATGTACTATTGAAGCTCGCTGATGATCCTAATATTGAAGTTAAGCTAAACTATGACAGTGTCAGGGAACTCACCCAGGATTTAATAGCGGAAGGCACTGATACCTTAGCGACCACTGTGGACTGGGCAATGTCCGAACTGATGAAACAACCACACCTCATCGAAAAAGCGATCGAAGAACTTGACAGAGTGATTGGGAGAGAGAGATGA

Protein Analysis

273

Amino Acids

31.54

Weight (kDa)

7.14

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 11 - 273 3.4e-34 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000606)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g08780 FvH4_1g08790 FvH4_1g08830 FvH4_6g25820
malus_domestica MD02G1092100.v1.1 MD02G1092500.v1.1 MD02G1093200.v1.1 MD15G1218600.v1.1
prunus_persica Prupe.7G197500_v2.0.a1 Prupe.7G197700_v2.0.a1 Prupe.7G197900_v2.0.a1 Prupe.7G198000_v2.0.a1 Prupe.7G198100_v2.0.a1 Prupe.7G198300_v2.0.a1
pyrus_communis pycom02g07340 pycom15g19370
rosa_chinensis RchiOBHm_Chr2g0094941 RchiOBHm_Chr2g0094971 RchiOBHm_Chr2g0095051 RchiOBHm_Chr2g0095221 RchiOBHm_Chr2g0095231 RchiOBHm_Chr2g0095261 RchiOBHm_Chr2g0103351 RchiOBHm_Chr2g0104551 RchiOBHm_Chr6g0297411
rosa_laevigata RLG00000016533 RLG00000016545 RLG00000016547 RLG00000016548 RLG00000017226
rosa_multiflora Rmu_sc0001555.1_g000008 Rmu_sc0003274.1_g000003 Rmu_sc0004316.1_g000013 Rmu_ssc0000183.1_g000015 Rmu_ssc0000183.1_g000017 Rmu_ssc0000183.1_g000023
rosa_roxburghii Rroxscaffold_2G00116650 Rroxscaffold_2G00139710 Rroxscaffold_2G00146510 Rroxscaffold_2G00146530 Rroxscaffold_2G00146690 Rroxscaffold_2G00146750 Rroxscaffold_3G00256890 Rroxscaffold_4G00297070 Rroxscaffold_4G00303160 Rroxscaffold_5G00374070 Rroxscaffold_6G00419510 Rroxscaffold_7G00186670 Rroxscaffold_7G00198970
rosa_rugosa Rorug01G0110700 Rorug02G0048900 Rorug02G0048900 Rorug02G0114200
rosa_samantha Rh1BG006000 Rh2AG162400 Rh2AG171200 Rh2BG096800 Rh2BG098400 Rh2CG098700 Rh2CG098900 Rh2CG100200 Rh2CG100400 Rh2CG169600 Rh2DG095400 Rh2DG095500 Rh2DG095600 Rh2DG098000 Rh2DG098100 Rh2DG168400 Rh4DG392900 Rh5DG019100 Rh5DG019200 Rh5DG460500 Rh6AG073600 Rh7DG484100
rosa_wichuraiana Rw2G007210 Rw2G007290 Rw2G007310 Rw2G007330 Rw2G012760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
AccB1I GGYRCC 2 cut(s) 139, 159
AciI CCGC 2 cut(s) 169, 701
AclWI GGATC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 4 cut(s) 107, 224, 356, 618
AgsI TTSAA 4 cut(s) 196, 448, 625, 650
AjnI CCWGG 2 cut(s) 467, 687
AloI GAACNNNNNNTCC 2 cut(s) 410, 442
AluBI AGCT 7 cut(s) 92, 98, 190, 262, 478, 628, 658
AluI AGCT 7 cut(s) 92, 98, 190, 262, 478, 628, 658
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 199, 371
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 3 cut(s) 10, 148, 166
ApeKI GCWGC 3 cut(s) 92, 95, 259
ApoI RAATTY 1 cut(s) 48
ArsI GACNNNNNNTTYG 2 cut(s) 536, 568
Asp700I GAANNNNTTC 2 cut(s) 356, 527
AspLEI GCGC 2 cut(s) 162, 248
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 280, 569, 676
AvaI CYCGRG 1 cut(s) 473
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 2 cut(s) 139, 159
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 79, 107, 271
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 469, 689
BciVI GTATCC 1 cut(s) 456
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 199, 371
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 163
BfuI GTATCC 1 cut(s) 456
BisI GCNGC 4 cut(s) 93, 96, 169, 260
BlsI GCNGC 4 cut(s) 94, 97, 170, 261
BmcAI AGTACT 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 469, 689
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 2 cut(s) 141, 161
BmrFI CCNGG 2 cut(s) 469, 689
BmrI ACTGGG 1 cut(s) 745
BmsI GCATC 1 cut(s) 402
BmuI ACTGGG 1 cut(s) 745
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 1 cut(s) 718
BpuEI CTTGAG 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 160
BsaJI CCNNGG 3 cut(s) 163, 467, 687
Bse1I ACTGG 2 cut(s) 284, 740
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 2 cut(s) 469, 689
BseDI CCNNGG 3 cut(s) 163, 467, 687
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 2 cut(s) 360, 366
BseNI ACTGG 2 cut(s) 284, 740
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 3 cut(s) 79, 107, 271
Bsh1285I CGRYCG 1 cut(s) 786
BshFI GGCC 3 cut(s) 12, 150, 168
BshNI GGYRCC 2 cut(s) 139, 159
BsiEI CGRYCG 1 cut(s) 786
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 9
BsmAI GTCTC 2 cut(s) 199, 371
BsnI GGCC 3 cut(s) 12, 150, 168
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 142, 303
Bsp143I GATC 4 cut(s) 64, 566, 637, 783
BspACI CCGC 2 cut(s) 169, 701
BspANI GGCC 3 cut(s) 12, 150, 168
BspCNI CTCAG 2 cut(s) 361, 367
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 2 cut(s) 141, 161
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 1 cut(s) 632
BspT107I GGYRCC 2 cut(s) 139, 159
BsrDI GCAATG 1 cut(s) 747
BsrI ACTGG 2 cut(s) 284, 740
BssECI CCNNGG 3 cut(s) 163, 467, 687
BssMI GATC 4 cut(s) 64, 566, 637, 783
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 163
Bst2UI CCWGG 2 cut(s) 469, 689
Bst4CI ACNGT 2 cut(s) 671, 730
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 160
BstC8I GCNNGC 3 cut(s) 100, 281, 630
BstDEI CTNAG 3 cut(s) 369, 375, 718
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 2 cut(s) 162, 248
BstKTI GATC 4 cut(s) 67, 569, 640, 786
BstMAI GTCTC 2 cut(s) 199, 371
BstMBI GATC 4 cut(s) 64, 566, 637, 783
BstMCI CGRYCG 1 cut(s) 786
BstMWI GCNNNNNNNGC 2 cut(s) 38, 168
BstNI CCWGG 2 cut(s) 469, 689
BstSCI CCNGG 2 cut(s) 467, 687
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 3 cut(s) 79, 107, 271
BsuI GTATCC 1 cut(s) 456
BsuRI GGCC 3 cut(s) 12, 150, 168
BtsIMutI CAGTG 3 cut(s) 676, 708, 726
Cac8I GCNNGC 3 cut(s) 100, 281, 630
CaiI CAGNNNCTG 1 cut(s) 415
CciI TCATGA 1 cut(s) 541
CfoI GCGC 2 cut(s) 162, 248
Cfr13I GGNCC 1 cut(s) 149
CseI GACGC 1 cut(s) 67
Csp6I GTAC 4 cut(s) 106, 223, 355, 617
CviAII CATG 4 cut(s) 62, 68, 542, 548
CviQI GTAC 4 cut(s) 106, 223, 355, 617
DdeI CTNAG 3 cut(s) 369, 375, 718
DinI GGCGCC 1 cut(s) 161
DpnI GATC 4 cut(s) 66, 568, 639, 785
DpnII GATC 4 cut(s) 64, 566, 637, 783
DraI TTTAAA 1 cut(s) 519
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 343
Eco88I CYCGRG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 467, 687
EcoT14I CCWWGG 1 cut(s) 163
EgeI GGCGCC 1 cut(s) 161
EheI GGCGCC 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 163
FaeI CATG 4 cut(s) 65, 71, 545, 551
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FatI CATG 4 cut(s) 61, 67, 541, 547
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 4 cut(s) 93, 96, 169, 260
Fsp4HI GCNGC 4 cut(s) 93, 96, 169, 260
FspBI CTAG 1 cut(s) 209
GlaI GCGC 2 cut(s) 161, 247
GluI GCNGC 4 cut(s) 93, 96, 169, 260
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 3 cut(s) 12, 150, 168
HapII CCGG 1 cut(s) 9
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 162, 248
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 4 cut(s) 65, 71, 545, 551
Hin6I GCGC 2 cut(s) 160, 246
HinP1I GCGC 2 cut(s) 160, 246
HinfI GANTC 2 cut(s) 212, 371
HpaII CCGG 1 cut(s) 9
HphI GGTGA 3 cut(s) 280, 569, 676
Hpy166II GTNNAC 3 cut(s) 108, 560, 733
Hpy188I TCNGA 4 cut(s) 187, 370, 376, 748
Hpy188III TCNNGA 2 cut(s) 81, 542
Hpy8I GTNNAC 3 cut(s) 108, 560, 733
Hpy99I CGWCG 1 cut(s) 401
HpyAV CCTTC 3 cut(s) 505, 572, 698
HpyCH4III ACNGT 2 cut(s) 671, 730
HpyCH4V TGCA 2 cut(s) 95, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 168
HpyF3I CTNAG 3 cut(s) 369, 375, 718
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 4 cut(s) 65, 71, 545, 551
HspAI GCGC 2 cut(s) 160, 246
KasI GGCGCC 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 64, 566, 637, 783
LmnI GCTCC 1 cut(s) 298
Lsp1109I GCAGC 3 cut(s) 79, 107, 271
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 286
MalI GATC 4 cut(s) 66, 568, 639, 785
MboI GATC 4 cut(s) 64, 566, 637, 783
MboII GAAGA 3 cut(s) 214, 361, 800
MhlI GDGCHC 2 cut(s) 142, 303
MluCI AATT 1 cut(s) 48
Mly113I GGCGCC 1 cut(s) 160
MlyI GAGTC 2 cut(s) 206, 380
MroXI GAANNNNTTC 2 cut(s) 356, 527
MseI TTAA 6 cut(s) 53, 435, 501, 518, 654, 695
MslI CAYNNNNRTG 3 cut(s) 66, 230, 546
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 469, 689
MvaI CCWGG 2 cut(s) 469, 689
MwoI GCNNNNNNNGC 2 cut(s) 38, 168
NarI GGCGCC 1 cut(s) 160
NdeII GATC 4 cut(s) 64, 566, 637, 783
NlaIII CATG 4 cut(s) 65, 71, 545, 551
NlaIV GGNNCC 2 cut(s) 141, 161
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 1 cut(s) 286
PaeR7I CTCGAG 1 cut(s) 473
PagI TCATGA 1 cut(s) 541
PdmI GAANNNNTTC 2 cut(s) 356, 527
PflFI GACNNNGTC 1 cut(s) 671
PkrI GCNGC 4 cut(s) 94, 97, 170, 261
Ple19I CGATCG 1 cut(s) 786
PleI GAGTC 2 cut(s) 206, 379
PluTI GGCGCC 1 cut(s) 163
PpsI GAGTC 2 cut(s) 206, 379
PsiI TTATAA 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 467, 687
PspGI CCWGG 2 cut(s) 467, 687
PspN4I GGNNCC 2 cut(s) 141, 161
PspPI GGNCC 1 cut(s) 149
PspXI VCTCGAGB 1 cut(s) 473
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 671
PvuI CGATCG 1 cut(s) 786
PvuII CAGCTG 1 cut(s) 98
RsaI GTAC 4 cut(s) 107, 224, 356, 618
RsaNI GTAC 4 cut(s) 106, 223, 355, 617
RseI CAYNNNNRTG 3 cut(s) 66, 230, 546
SaqAI TTAA 6 cut(s) 53, 435, 501, 518, 654, 695
SatI GCNGC 4 cut(s) 93, 96, 169, 260
Sau3AI GATC 4 cut(s) 64, 566, 637, 783
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 206, 380
ScrFI CCNGG 2 cut(s) 469, 689
SduI GDGCHC 2 cut(s) 142, 303
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 93
SfoI GGCGCC 1 cut(s) 161
Sfr274I CTCGAG 1 cut(s) 473
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 3 cut(s) 66, 230, 546
SmlI CTYRAG 2 cut(s) 293, 473
SmoI CTYRAG 2 cut(s) 293, 473
Sse9I AATT 1 cut(s) 48
SsiI CCGC 2 cut(s) 169, 701
SspDI GGCGCC 1 cut(s) 159
SspI AATATT 1 cut(s) 646
SspMI CTAG 1 cut(s) 209
StyD4I CCNGG 2 cut(s) 467, 687
StyI CCWWGG 1 cut(s) 163
TaaI ACNGT 2 cut(s) 671, 730
TaqI TCGA 7 cut(s) 200, 383, 396, 474, 531, 774, 786
TasI AATT 1 cut(s) 48
TatI WGTACW 4 cut(s) 105, 222, 354, 616
TauI GCSGC 1 cut(s) 171
Tru1I TTAA 6 cut(s) 53, 435, 501, 518, 654, 695
Tru9I TTAA 6 cut(s) 53, 435, 501, 518, 654, 695
TscAI CASTG 3 cut(s) 676, 715, 733
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 3 cut(s) 92, 95, 259
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 17, 524, 530, 770
TspRI CASTG 3 cut(s) 676, 715, 733
Tth111I GACNNNGTC 1 cut(s) 671
XapI RAATTY 1 cut(s) 48
XcmI CCANNNNNNNNNTGG 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 473
XmnI GAANNNNTTC 2 cut(s) 356, 527
XspI CTAG 1 cut(s) 209
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.