Rh5AG033600

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002193
Physical Location & Seq
Reverse (-)
2278580 .. 2278996
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG033600.1

Sequence Viewer

Length: 336 bp
ATGCATGAGCTTCTAGGAGCTGATTTGCCTGTGTCTTCCAAGTTTGATCTGGAGATGCTTGAGAATGGATTAGACTTCATCGGTATCAATCACCATACCAGTTTTTATGCAAAAGACTGTCTGTTTTCTCCATGTGAACCAGGACCAGGAGCTGCAAGGATAAAGGGGTATACTTTGAGAACTGAACAAAAAGATGGAGTCTACATTGGAGAACCAACTACAATTGATTGGCTATATGTGTGTCCTCAAGGAATGGAGAAGATGGTAACATACATAAAAGAAAGATACAACAAACCAATGTTTATTACAGAAAATGGTAAGCTAACAATTCTTTGA

Protein Analysis

111

Amino Acids

12.59

Weight (kDa)

5.28

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 9 - 106 3.4e-09 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 170, 201
AfiI CCNNNNNNNGG 1 cut(s) 146
AjnI CCWGG 2 cut(s) 139, 145
AluBI AGCT 4 cut(s) 10, 20, 152, 322
AluI AGCT 4 cut(s) 10, 20, 152, 322
AlwNI CAGNNNCTG 1 cut(s) 152
ApeKI GCWGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 83
AvaII GGWCC 1 cut(s) 143
BbsI GAAGAC 1 cut(s) 27
BbvI GCAGC 1 cut(s) 139
BccI CCATC 2 cut(s) 188, 256
BciT130I CCWGG 2 cut(s) 141, 147
BfaI CTAG 1 cut(s) 14
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 2 cut(s) 141, 147
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 141, 147
BmsI GCATC 1 cut(s) 45
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 2 cut(s) 80, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 141, 147
BseLI CCNNNNNNNGG 1 cut(s) 146
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 139
BslI CCNNNNNNNGG 1 cut(s) 146
Bsp143I GATC 1 cut(s) 46
BsrI ACTGG 1 cut(s) 99
BssMI GATC 1 cut(s) 46
BssNAI GTATAC 1 cut(s) 171
Bst1107I GTATAC 1 cut(s) 171
Bst2UI CCWGG 2 cut(s) 141, 147
Bst4CI ACNGT 1 cut(s) 119
BstKTI GATC 1 cut(s) 49
BstMBI GATC 1 cut(s) 46
BstNI CCWGG 2 cut(s) 141, 147
BstSCI CCNGG 2 cut(s) 139, 145
BstV1I GCAGC 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 27
BstZ17I GTATAC 1 cut(s) 171
CaiI CAGNNNCTG 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 143
CviAII CATG 2 cut(s) 5, 132
CviJI RGCY 5 cut(s) 10, 20, 152, 232, 322
CviKI_1 RGCY 5 cut(s) 10, 20, 152, 232, 322
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 139, 145
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 8, 135
FaiI YATR 9 cut(s) 6, 96, 108, 133, 171, 235, 237, 271, 275
FatI CATG 2 cut(s) 4, 131
FblI GTMKAC 2 cut(s) 170, 201
Fnu4HI GCNGC 1 cut(s) 153
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 1 cut(s) 14
GluI GCNGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 71
Hin1II CATG 2 cut(s) 8, 135
HinfI GANTC 1 cut(s) 198
HphI GGTGA 1 cut(s) 83
Hpy166II GTNNAC 3 cut(s) 137, 171, 202
Hpy188III TCNNGA 1 cut(s) 50
Hpy8I GTNNAC 3 cut(s) 137, 171, 202
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 3 cut(s) 4, 110, 155
Hsp92II CATG 2 cut(s) 8, 135
Kzo9I GATC 1 cut(s) 46
LmnI GCTCC 2 cut(s) 17, 149
LpnPI CCDG 7 cut(s) 35, 42, 112, 126, 132, 153, 159
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 45
MaeI CTAG 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 2 cut(s) 27, 271
MfeI CAATTG 1 cut(s) 222
MluCI AATT 2 cut(s) 222, 327
MlyI GAGTC 1 cut(s) 207
MnlI CCTC 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 6
MspR9I CCNGG 2 cut(s) 141, 147
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 2 cut(s) 141, 147
NdeII GATC 1 cut(s) 46
NlaIII CATG 2 cut(s) 8, 135
NsiI ATGCAT 1 cut(s) 6
PkrI GCNGC 1 cut(s) 154
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 139, 145
PspGI CCWGG 2 cut(s) 139, 145
PspPI GGNCC 1 cut(s) 143
PstNI CAGNNNCTG 1 cut(s) 152
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 141, 147
SetI ASST 4 cut(s) 12, 22, 154, 324
SfaNI GCATC 1 cut(s) 45
SinI GGWCC 1 cut(s) 143
SmlI CTYRAG 2 cut(s) 59, 246
SmoI CTYRAG 2 cut(s) 59, 246
Sse9I AATT 2 cut(s) 222, 327
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 139, 145
TaaI ACNGT 1 cut(s) 119
TasI AATT 2 cut(s) 222, 327
TseI GCWGC 1 cut(s) 152
TspDTI ATGAA 1 cut(s) 67
VpaK11BI GGWCC 1 cut(s) 143
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XmiI GTMKAC 2 cut(s) 170, 201
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.