Rh5BG032300

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002193
Physical Location & Seq
Reverse (-)
2253810 .. 2255195
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG032300.1

Sequence Viewer

Length: 798 bp
ATGTCTGTAACACAGAAAAAACAAGGAGGTAGCATTGGGATGGTGATGAATGCTGTATGGTATGAACCCATCAGCAACTCCTTAGAAGACAGATTAGCAGCTGAGAGGGCTCAGTCTTTCTATATCAACTGGTTCTTAGACCCAATTCTCCATGGAAGATACCCTGCAGAAATGCATGAGCTTCTAGGAGCTGATTTGCCTGTATTTTCCAAATTTGATCTGGAGATGCTGAAGAATGGATTAGACTTCATCGGTATCAATCACTACACCAGTTTTTACTCAAAAGACTGTATGTTTTCTCCATGTGAACCAGGACCAGGAGCTTCAAGGACAGAGGGTTATGCTTTGAGAACTGCACAAAAAGATGGAGTCTACATTGGAGAACCAACTTCAGTTGATTGGATATATGTGTATCCTCAAGGAATGGAGAAGATGGTAACATACATAAAAGAGAGGTACAATAATACACCAATGTTTATTACAGAAAATGGGTATGGTGTGGAGGAGAATCCAAATTCCACAAATGAAGAACTGTTCCATGATGTCAAGAGAGTGGAGTACTTGAGAAGTTACTTACAGGCACTAGCAGATACAATGAGAACAAAAGGAGCAGATGTAAGAGGGTACTTCGTGTGGTCATTGCTTGATAATTTCGAGTGGACAAGTGGATATACGGTCAAGTTTGGACTTCACCATGTTGATTATGGCACTCTCAAGAGAACTCCAAGACTCTCTGCAGCTTGGTACAGACAATTTATTGCTAATAACACCCTGCAGATTTTAAGTGCCCAGGAATGA

Protein Analysis

265

Amino Acids

30.47

Weight (kDa)

5.45

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 4 - 256 2.6e-64 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 372
AcsI RAATTY 2 cut(s) 212, 514
AcuI CTGAAG 2 cut(s) 251, 375
AfaI GTAC 4 cut(s) 458, 560, 626, 746
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 1 cut(s) 327
AjnI CCWGG 3 cut(s) 310, 316, 789
AluBI AGCT 5 cut(s) 101, 181, 191, 323, 740
AluI AGCT 5 cut(s) 101, 181, 191, 323, 740
ApeKI GCWGC 2 cut(s) 98, 737
ApoI RAATTY 2 cut(s) 212, 514
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 2 cut(s) 55, 683
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 790
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 110, 749
BccI CCATC 4 cut(s) 34, 77, 359, 427
BciT130I CCWGG 3 cut(s) 312, 318, 791
BciVI GTATCC 1 cut(s) 423
BfaI CTAG 2 cut(s) 185, 584
BfmI CTRYAG 3 cut(s) 165, 735, 773
BfuI GTATCC 1 cut(s) 423
BisI GCNGC 2 cut(s) 99, 738
BlsI GCNGC 2 cut(s) 100, 739
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 3 cut(s) 312, 318, 791
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 314
BmrFI CCNGG 3 cut(s) 312, 318, 791
BmsI GCATC 1 cut(s) 216
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 242
BpuEI CTTGAG 3 cut(s) 402, 583, 698
BsaJI CCNNGG 2 cut(s) 151, 789
BsaXI ACNNNNNCTCC 2 cut(s) 600, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 134, 270
Bse3DI GCAATG 1 cut(s) 638
BseBI CCWGG 3 cut(s) 312, 318, 791
BseDI CCNNGG 2 cut(s) 151, 789
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 2 cut(s) 93, 125
BseNI ACTGG 2 cut(s) 134, 270
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 2 cut(s) 110, 749
BsgI GTGCAG 1 cut(s) 339
BslI CCNNNNNNNGG 1 cut(s) 317
BsmI GAATGC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 112, 790
Bsp143I GATC 1 cut(s) 217
Bsp19I CCATGG 1 cut(s) 151
BspCNI CTCAG 2 cut(s) 94, 124
BspMAI CTGCAG 3 cut(s) 169, 739, 777
BsrDI GCAATG 1 cut(s) 638
BsrI ACTGG 2 cut(s) 134, 270
BssECI CCNNGG 2 cut(s) 151, 789
BssMI GATC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 3 cut(s) 312, 318, 791
Bst4CI ACNGT 3 cut(s) 290, 534, 676
BstDEI CTNAG 4 cut(s) 82, 102, 111, 136
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 1 cut(s) 220
BstMBI GATC 1 cut(s) 217
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 3 cut(s) 312, 318, 791
BstSCI CCNGG 3 cut(s) 310, 316, 789
BstSFI CTRYAG 3 cut(s) 165, 735, 773
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 2 cut(s) 110, 749
BstV2I GAAGAC 1 cut(s) 93
BsuI GTATCC 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 314
Csp6I GTAC 4 cut(s) 457, 559, 625, 745
CviAII CATG 5 cut(s) 152, 176, 303, 539, 695
CviJI RGCY 6 cut(s) 101, 110, 181, 191, 323, 740
CviKI_1 RGCY 6 cut(s) 101, 110, 181, 191, 323, 740
CviQI GTAC 4 cut(s) 457, 559, 625, 745
DdeI CTNAG 4 cut(s) 82, 102, 111, 136
DpnI GATC 1 cut(s) 219
DpnII GATC 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 112
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 2 cut(s) 251, 375
EcoRII CCWGG 3 cut(s) 310, 316, 789
EcoT14I CCWWGG 1 cut(s) 151
EcoT22I ATGCAT 1 cut(s) 177
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 5 cut(s) 155, 179, 306, 542, 698
FatI CATG 5 cut(s) 151, 175, 302, 538, 694
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 2 cut(s) 99, 738
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 2 cut(s) 99, 738
FspBI CTAG 2 cut(s) 185, 584
GluI GCNGC 2 cut(s) 99, 738
GsuI CTGGAG 1 cut(s) 242
Hin1II CATG 5 cut(s) 155, 179, 306, 542, 698
HinfI GANTC 3 cut(s) 369, 508, 729
HphI GGTGA 2 cut(s) 55, 683
Hpy166II GTNNAC 3 cut(s) 308, 373, 660
Hpy188III TCNNGA 3 cut(s) 221, 547, 715
Hpy8I GTNNAC 3 cut(s) 308, 373, 660
HpyCH4III ACNGT 3 cut(s) 290, 534, 676
HpyCH4V TGCA 5 cut(s) 167, 175, 356, 737, 775
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 4 cut(s) 82, 102, 111, 136
Hsp92II CATG 5 cut(s) 155, 179, 306, 542, 698
Kzo9I GATC 1 cut(s) 217
LmnI GCTCC 3 cut(s) 188, 320, 608
Lsp1109I GCAGC 2 cut(s) 110, 749
LweI GCATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 185, 584
MaeIII GTNAC 3 cut(s) 7, 436, 569
MalI GATC 1 cut(s) 219
MboI GATC 1 cut(s) 217
MboII GAAGA 5 cut(s) 98, 168, 244, 442, 539
MhlI GDGCHC 2 cut(s) 112, 790
MluCI AATT 5 cut(s) 144, 212, 514, 649, 752
MlyI GAGTC 2 cut(s) 378, 723
MnlI CCTC 7 cut(s) 20, 99, 328, 426, 447, 496, 614
Mph1103I ATGCAT 1 cut(s) 177
MseI TTAA 1 cut(s) 782
MslI CAYNNNNRTG 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 101
MspR9I CCNGG 3 cut(s) 312, 318, 791
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 3 cut(s) 312, 318, 791
MwoI GCNNNNNNNGC 1 cut(s) 107
NcoI CCATGG 1 cut(s) 151
NdeII GATC 1 cut(s) 217
NlaIII CATG 5 cut(s) 155, 179, 306, 542, 698
NsiI ATGCAT 1 cut(s) 177
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 1 cut(s) 508
PkrI GCNGC 2 cut(s) 100, 739
PleI GAGTC 2 cut(s) 377, 723
PpsI GAGTC 2 cut(s) 377, 723
Psp6I CCWGG 3 cut(s) 310, 316, 789
PspGI CCWGG 3 cut(s) 310, 316, 789
PspPI GGNCC 1 cut(s) 314
PstI CTGCAG 3 cut(s) 169, 739, 777
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 4 cut(s) 458, 560, 626, 746
RsaNI GTAC 4 cut(s) 457, 559, 625, 745
RseI CAYNNNNRTG 1 cut(s) 38
SaqAI TTAA 1 cut(s) 782
SatI GCNGC 2 cut(s) 99, 738
Sau3AI GATC 1 cut(s) 217
Sau96I GGNCC 1 cut(s) 314
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 2 cut(s) 378, 723
ScrFI CCNGG 3 cut(s) 312, 318, 791
SduI GDGCHC 2 cut(s) 112, 790
SetI ASST 7 cut(s) 31, 103, 183, 193, 325, 458, 742
SfaNI GCATC 1 cut(s) 216
SfcI CTRYAG 3 cut(s) 165, 735, 773
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 3 cut(s) 417, 562, 713
SmoI CTYRAG 3 cut(s) 417, 562, 713
Sse9I AATT 5 cut(s) 144, 212, 514, 649, 752
SspMI CTAG 2 cut(s) 185, 584
StyD4I CCNGG 3 cut(s) 310, 316, 789
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 3 cut(s) 290, 534, 676
TaqI TCGA 1 cut(s) 654
TasI AATT 5 cut(s) 144, 212, 514, 649, 752
TatI WGTACW 1 cut(s) 558
TfiI GAWTC 1 cut(s) 508
Tru1I TTAA 1 cut(s) 782
Tru9I TTAA 1 cut(s) 782
TseI GCWGC 2 cut(s) 98, 737
TspDTI ATGAA 4 cut(s) 62, 78, 238, 540
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 2 cut(s) 212, 514
XcmI CCANNNNNNNNNTGG 2 cut(s) 217, 701
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 2 cut(s) 185, 584
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.