Rw5G003110

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002193
Physical Location & Seq
Reverse (-)
2883715 .. 2887096
3382 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003110.1

Sequence Viewer

Length: 1545 bp
ATGGAGGCTGTATCAGCATTTCATGCTTTCCTCATTATTGAAATCATGTGTTTTTCGATTCACATAGTGTCATGTAATCACATATCCTTGAAAGGAAACTCGCCGAGCAACTTTCTCTTTGGAACTGCATCCTCTTCTTATCAGTTTGAAGGAGCTTTTCTGAATGATGGTAAAGGGCTCAACAACTGGGATGTCTTCACTCACAAGCCTGGTACTATCCTGGATGGAACTAATGGAGACACTGCTGTTGATCAGTACCATCTTTATAAGGAAGACTTGGGTCTCATGAACAACATTGGAGTCAACAGCTACCGTCTTTCTATATCATGGGCAAGAATTTTACCCAAAGGAAGATTTGGAAAAGTGAACAGAGCTGGCATTGATCACTATAACAAGTTCATCGACGCTCTTCTGCTTAGAGGAATCCAGCCCTTTGTGACATTGACCCACTACGACATACCTCAGGAACTTGAAGATAGATATGGAGCTTGGCTAAGTCCCCTACTGCAGGAAGATTTTAGATATTATGCAAATATATGTTTCAAATTCTTTGGAGACAGAGTCAAGTACTGGTCCACTTTCAATGAGCCCAATGTTGTGGTCATTCGTGGATATAGGTCAGGGATTTACCCACCATCTCATTGCTCTCTTCCTTTCGGAAATTGTACTTCTGGAGACTCAGAAAGAGAGCCTTTCATTGCAGCTCATAACATCATTCTATCCCATGCAGCTGCTGTGAATATATACCGAACCAAATATCAGAAAAAACAAGGAGGTAGCATTGGGATGGTGATGAATGCTATATGGTATGAACCCATCAGCAACTCCTTAGAAGACAGATTAGCAGCTGAGAGGGCTCAGTCTTTCTATATCAACTGGTTCTTAGACCCAATTCTCCATGGAAGATACCCTGCAGAAATGCATGAGCTTCTAGGAGCTGATTTGCCTGTATTTTCCAAATTTGATCTGGAGATGCTGAAGAATGGATTAGACTTCATCGGTATCAATCACTACACCAGTTTTTACTCAAAAGACTGTATGTTTTCTCCATGTGAACCAGGACCAGGAGCTTCAAGGACAGAGGGTTATGCTTTGAGAACTGCACAAAAAGATGGAGTCTACATTGGAGAACCAACTTCAGTTGATTGGATATATGTGTATCCTCAAGGAATGGAGAAGATGGTAACATACATAAAAGAGAGGTACAATAATACACCAATGTTTATTACAGAAAATGGGTATGGTGTGGAGGAGAATCCAAATTCCACAAATGAAGAACTGTTCCACGATGTCAAGAGAGTGGAGTACTTGAGAAGGTACTTACAGGCACTAGCAGATACAATGAGAACTAAAGGAGCAGATGTAAGAGGGTACTTTGTGTGGTCATTGCTTGATGATTTCGAGTGGATAAGTGGATATACGGTCAAGTTTGGACTTCACCACGTTGATTATGGCACTCTCAAGAGAACTCCAAGACTCTCTGCAGCTTGGTACAGACAGTTTATTGCTAATAACACCCTGCAGATTTTAAGTGCCCAGGAATGA

Protein Analysis

514

Amino Acids

58.79

Weight (kDa)

6.07

Isoelectric Point (pI)

37.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 34 - 505 2e-150 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccI GTMKAC 1 cut(s) 1119
AcsI RAATTY 4 cut(s) 336, 545, 959, 1261
AcuI CTGAAG 2 cut(s) 998, 1122
AdeI CACNNNGTG 1 cut(s) 67
AfaI GTAC 9 cut(s) 214, 257, 569, 667, 1205, 1307, 1319, 1373, 1493
AfiI CCNNNNNNNGG 2 cut(s) 508, 1064
AgsI TTSAA 7 cut(s) 41, 91, 149, 473, 544, 583, 1074
AjnI CCWGG 5 cut(s) 208, 219, 1057, 1063, 1536
Alw26I GTCTC 4 cut(s) 231, 287, 549, 669
AlwNI CAGNNNCTG 1 cut(s) 734
ApeKI GCWGC 5 cut(s) 701, 728, 731, 845, 1484
ApoI RAATTY 4 cut(s) 336, 545, 959, 1261
AspS9I GGNCC 2 cut(s) 573, 1061
AsuHPI GGTGA 2 cut(s) 802, 1430
AvaII GGWCC 2 cut(s) 573, 1061
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 1537
BanII GRGCYC 3 cut(s) 180, 591, 859
BbsI GAAGAC 3 cut(s) 187, 279, 840
BbvI GCAGC 5 cut(s) 713, 718, 740, 857, 1496
BccI CCATC 8 cut(s) 161, 218, 267, 643, 781, 824, 1106, 1174
BciT130I CCWGG 5 cut(s) 210, 221, 1059, 1065, 1538
BciVI GTATCC 1 cut(s) 1170
BclI TGATCA 2 cut(s) 250, 382
BcoDI GTCTC 4 cut(s) 231, 287, 549, 669
BfaI CTAG 2 cut(s) 932, 1331
BfmI CTRYAG 4 cut(s) 506, 912, 1482, 1520
BfuI GTATCC 1 cut(s) 1170
BisI GCNGC 5 cut(s) 702, 729, 732, 846, 1485
BlsI GCNGC 5 cut(s) 703, 730, 733, 847, 1486
BmcAI AGTACT 2 cut(s) 569, 1307
Bme1390I CCNGG 5 cut(s) 210, 221, 1059, 1065, 1538
Bme18I GGWCC 2 cut(s) 573, 1061
BmgT120I GGNCC 2 cut(s) 573, 1061
BmrFI CCNGG 5 cut(s) 210, 221, 1059, 1065, 1538
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 2 cut(s) 137, 963
BmuI ACTGGG 1 cut(s) 196
BoxI GACNNNNGTC 1 cut(s) 279
BpiI GAAGAC 3 cut(s) 187, 279, 840
BpmI CTGGAG 2 cut(s) 693, 989
BpuEI CTTGAG 3 cut(s) 1149, 1330, 1445
BsaI GGTCTC 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 898, 1536
BsaXI ACNNNNNCTCC 4 cut(s) 546, 576, 1347, 1377
Bsc4I CCNNNNNNNGG 2 cut(s) 508, 1064
Bse1I ACTGG 4 cut(s) 191, 575, 881, 1017
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 3 cut(s) 640, 696, 1385
BseBI CCWGG 5 cut(s) 210, 221, 1059, 1065, 1538
BseDI CCNNGG 2 cut(s) 898, 1536
BseGI GGATG 4 cut(s) 128, 196, 229, 792
BseLI CCNNNNNNNGG 2 cut(s) 508, 1064
BseMI GCAATG 3 cut(s) 640, 696, 1385
BseMII CTCAG 4 cut(s) 476, 693, 840, 872
BseNI ACTGG 4 cut(s) 191, 575, 881, 1017
BseRI GAGGAG 1 cut(s) 1265
BseSI GKGCMC 1 cut(s) 1537
BseXI GCAGC 5 cut(s) 713, 718, 740, 857, 1496
BsgI GTGCAG 1 cut(s) 1086
BslFI GGGAC 1 cut(s) 483
BslI CCNNNNNNNGG 2 cut(s) 508, 1064
BsmAI GTCTC 4 cut(s) 231, 287, 549, 669
BsmFI GGGAC 1 cut(s) 483
BsmI GAATGC 1 cut(s) 802
Bso31I GGTCTC 1 cut(s) 287
Bsp1286I GDGCHC 4 cut(s) 180, 591, 859, 1537
Bsp143I GATC 3 cut(s) 250, 382, 964
Bsp19I CCATGG 1 cut(s) 898
BspCNI CTCAG 4 cut(s) 475, 692, 841, 871
BspHI TCATGA 1 cut(s) 285
BspMAI CTGCAG 4 cut(s) 510, 916, 1486, 1524
BspQI GCTCTTC 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 287
BsrDI GCAATG 3 cut(s) 640, 696, 1385
BsrI ACTGG 4 cut(s) 191, 575, 881, 1017
BssECI CCNNGG 2 cut(s) 898, 1536
BssMI GATC 3 cut(s) 250, 382, 964
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 5 cut(s) 210, 221, 1059, 1065, 1538
Bst4CI ACNGT 5 cut(s) 314, 1037, 1281, 1423, 1500
Bst6I CTCTTC 3 cut(s) 139, 414, 654
BstAPI GCANNNNNTGC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 8 cut(s) 416, 462, 494, 679, 829, 849, 858, 883
BstDSI CCRYGG 1 cut(s) 898
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 4 cut(s) 128, 196, 229, 792
BstKTI GATC 3 cut(s) 253, 385, 967
BstMAI GTCTC 4 cut(s) 231, 287, 549, 669
BstMBI GATC 3 cut(s) 250, 382, 964
BstMWI GCNNNNNNNGC 3 cut(s) 14, 23, 854
BstNI CCWGG 5 cut(s) 210, 221, 1059, 1065, 1538
BstPAI GACNNNNGTC 1 cut(s) 279
BstSCI CCNGG 5 cut(s) 208, 219, 1057, 1063, 1536
BstSFI CTRYAG 4 cut(s) 506, 912, 1482, 1520
BstSLI GKGCMC 1 cut(s) 1537
BstV1I GCAGC 5 cut(s) 713, 718, 740, 857, 1496
BstV2I GAAGAC 3 cut(s) 187, 279, 840
BstXI CCANNNNNNTGG 1 cut(s) 598
Bsu36I CCTNAGG 1 cut(s) 462
BsuI GTATCC 1 cut(s) 1170
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 4 cut(s) 128, 196, 229, 792
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 376
CaiI CAGNNNCTG 1 cut(s) 734
CciI TCATGA 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 573, 1061
CseI GACGC 1 cut(s) 413
Csp6I GTAC 9 cut(s) 213, 256, 568, 666, 1204, 1306, 1318, 1372, 1492
CviAII CATG 9 cut(s) 23, 46, 72, 286, 327, 725, 899, 923, 1050
CviQI GTAC 9 cut(s) 213, 256, 568, 666, 1204, 1306, 1318, 1372, 1492
DdeI CTNAG 8 cut(s) 416, 462, 494, 679, 829, 849, 858, 883
DpnI GATC 3 cut(s) 252, 384, 966
DpnII GATC 3 cut(s) 250, 382, 964
DraIII CACNNNGTG 1 cut(s) 67
Eam1104I CTCTTC 3 cut(s) 139, 414, 654
EarI CTCTTC 3 cut(s) 139, 414, 654
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 3 cut(s) 180, 591, 859
Eco31I GGTCTC 1 cut(s) 287
Eco47I GGWCC 2 cut(s) 573, 1061
Eco57I CTGAAG 2 cut(s) 998, 1122
Eco81I CCTNAGG 1 cut(s) 462
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoRII CCWGG 5 cut(s) 208, 219, 1057, 1063, 1536
EcoT14I CCWWGG 1 cut(s) 898
EcoT22I ATGCAT 1 cut(s) 924
EcoT38I GRGCYC 3 cut(s) 180, 591, 859
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 9 cut(s) 26, 49, 75, 289, 330, 728, 902, 926, 1053
FalI AAGNNNNNCTT 4 cut(s) 260, 292, 676, 708
FaqI GGGAC 1 cut(s) 483
FatI CATG 9 cut(s) 22, 45, 71, 285, 326, 724, 898, 922, 1049
FbaI TGATCA 2 cut(s) 250, 382
FblI GTMKAC 1 cut(s) 1119
Fnu4HI GCNGC 5 cut(s) 702, 729, 732, 846, 1485
FokI GGATG 4 cut(s) 115, 203, 236, 799
FriOI GRGCYC 3 cut(s) 180, 591, 859
Fsp4HI GCNGC 5 cut(s) 702, 729, 732, 846, 1485
FspBI CTAG 2 cut(s) 932, 1331
GluI GCNGC 5 cut(s) 702, 729, 732, 846, 1485
GsuI CTGGAG 2 cut(s) 693, 989
HgaI GACGC 1 cut(s) 413
Hin1II CATG 9 cut(s) 26, 49, 75, 289, 330, 728, 902, 926, 1053
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HinfI GANTC 8 cut(s) 58, 300, 423, 561, 677, 1116, 1255, 1476
HphI GGTGA 2 cut(s) 802, 1430
Hpy166II GTNNAC 5 cut(s) 304, 367, 576, 1055, 1120
Hpy188I TCNGA 4 cut(s) 162, 659, 682, 762
Hpy188III TCNNGA 6 cut(s) 286, 464, 672, 968, 1294, 1462
Hpy8I GTNNAC 5 cut(s) 304, 367, 576, 1055, 1120
Hpy99I CGWCG 1 cut(s) 407
HpyAV CCTTC 2 cut(s) 143, 1308
HpyCH4III ACNGT 5 cut(s) 314, 1037, 1281, 1423, 1500
HpyCH4IV ACGT 1 cut(s) 1443
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 23, 854
HpyF3I CTNAG 8 cut(s) 416, 462, 494, 679, 829, 849, 858, 883
HpySE526I ACGT 1 cut(s) 1443
Hsp92II CATG 9 cut(s) 26, 49, 75, 289, 330, 728, 902, 926, 1053
Ksp22I TGATCA 2 cut(s) 250, 382
Kzo9I GATC 3 cut(s) 250, 382, 964
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 5 cut(s) 152, 485, 935, 1067, 1355
Lsp1109I GCAGC 5 cut(s) 713, 718, 740, 857, 1496
LweI GCATC 2 cut(s) 137, 963
MaeI CTAG 2 cut(s) 932, 1331
MaeII ACGT 1 cut(s) 1443
MaeIII GTNAC 2 cut(s) 436, 1183
MalI GATC 3 cut(s) 252, 384, 966
MboI GATC 3 cut(s) 250, 382, 964
MhlI GDGCHC 4 cut(s) 180, 591, 859, 1537
MluCI AATT 6 cut(s) 336, 545, 661, 891, 959, 1261
MlyI GAGTC 5 cut(s) 309, 570, 671, 1125, 1470
Mph1103I ATGCAT 1 cut(s) 924
MseI TTAA 1 cut(s) 1529
MslI CAYNNNNRTG 1 cut(s) 785
MspA1I CMGCKG 2 cut(s) 731, 848
MspR9I CCNGG 5 cut(s) 210, 221, 1059, 1065, 1538
Mva1269I GAATGC 1 cut(s) 802
MvaI CCWGG 5 cut(s) 210, 221, 1059, 1065, 1538
MwoI GCNNNNNNNGC 3 cut(s) 14, 23, 854
NcoI CCATGG 1 cut(s) 898
NdeII GATC 3 cut(s) 250, 382, 964
NlaIII CATG 9 cut(s) 26, 49, 75, 289, 330, 728, 902, 926, 1053
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 924
PagI TCATGA 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 414
PctI GAATGC 1 cut(s) 802
PfeI GAWTC 3 cut(s) 58, 423, 1255
PflFI GACNNNGTC 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 5 cut(s) 703, 730, 733, 847, 1486
PleI GAGTC 5 cut(s) 308, 569, 671, 1124, 1470
PpsI GAGTC 5 cut(s) 308, 569, 671, 1124, 1470
PshAI GACNNNNGTC 1 cut(s) 279
PsiI TTATAA 1 cut(s) 267
Psp6I CCWGG 5 cut(s) 208, 219, 1057, 1063, 1536
PspGI CCWGG 5 cut(s) 208, 219, 1057, 1063, 1536
PspPI GGNCC 2 cut(s) 573, 1061
PstI CTGCAG 4 cut(s) 510, 916, 1486, 1524
PstNI CAGNNNCTG 1 cut(s) 734
PsyI GACNNNGTC 1 cut(s) 560
PvuII CAGCTG 2 cut(s) 731, 848
RsaI GTAC 9 cut(s) 214, 257, 569, 667, 1205, 1307, 1319, 1373, 1493
RsaNI GTAC 9 cut(s) 213, 256, 568, 666, 1204, 1306, 1318, 1372, 1492
RseI CAYNNNNRTG 1 cut(s) 785
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 1 cut(s) 1529
SatI GCNGC 5 cut(s) 702, 729, 732, 846, 1485
Sau3AI GATC 3 cut(s) 250, 382, 964
Sau96I GGNCC 2 cut(s) 573, 1061
ScaI AGTACT 2 cut(s) 569, 1307
SchI GAGTC 5 cut(s) 309, 570, 671, 1125, 1470
ScrFI CCNGG 5 cut(s) 210, 221, 1059, 1065, 1538
SduI GDGCHC 4 cut(s) 180, 591, 859, 1537
SfaNI GCATC 2 cut(s) 137, 963
SfcI CTRYAG 4 cut(s) 506, 912, 1482, 1520
SinI GGWCC 2 cut(s) 573, 1061
SmiMI CAYNNNNRTG 1 cut(s) 785
SmlI CTYRAG 3 cut(s) 1164, 1309, 1460
SmoI CTYRAG 3 cut(s) 1164, 1309, 1460
Sse9I AATT 6 cut(s) 336, 545, 661, 891, 959, 1261
SspMI CTAG 2 cut(s) 932, 1331
StyD4I CCNGG 5 cut(s) 208, 219, 1057, 1063, 1536
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 5 cut(s) 314, 1037, 1281, 1423, 1500
TaiI ACGT 1 cut(s) 1446
TaqI TCGA 3 cut(s) 56, 402, 1401
TasI AATT 6 cut(s) 336, 545, 661, 891, 959, 1261
TatI WGTACW 3 cut(s) 567, 665, 1305
TfiI GAWTC 3 cut(s) 58, 423, 1255
Tru1I TTAA 1 cut(s) 1529
Tru9I TTAA 1 cut(s) 1529
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 5 cut(s) 701, 728, 731, 845, 1484
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 8 cut(s) 11, 302, 388, 685, 809, 825, 985, 1287
TspRI CASTG 1 cut(s) 247
Tth111I GACNNNGTC 1 cut(s) 560
VpaK11BI GGWCC 2 cut(s) 573, 1061
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 4 cut(s) 336, 545, 959, 1261
XcmI CCANNNNNNNNNTGG 2 cut(s) 964, 1448
XmiI GTMKAC 1 cut(s) 1119
XspI CTAG 2 cut(s) 932, 1331
ZrmI AGTACT 2 cut(s) 569, 1307
Zsp2I ATGCAT 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.