Rh5BG032400

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002193
Physical Location & Seq
Reverse (-)
2255716 .. 2257583
1868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG032400.1

Sequence Viewer

Length: 417 bp
ATGTGTTTTTCGATTCTCGTAGTGTCATGTAATCACATATCCTTGAAAGAAAACTCGCCGAGCAACTTTCTCTTTGGAACTGCATCCTCTTCTTATCAGTTTGAAGGAGCTTTTCTGAATGATGGTAAAGGTCTCAACAACTGGGATATCTTCACTCACAAGCCTGTCTTCACATATCTAAGCAATCTCTACTATAACTATGAACCAGAAGGAAGATTTGGAAAAGTGAACAGAGCTGGCATTGATCACTATAACAAGTTCATCGATGCTCTTCTGCTTAGAGGTCTTCTTTGTTACTATTGTTTATGTACTCATCCAATTTATTTAAGTTGCTGCATTACTAAAGTGTTGTCAGAAAACTTGTGTTCAATGCTCATACACAAGTATGACATGTATCTCCTTCCTCAACATATTTAG

Protein Analysis

138

Amino Acids

15.9

Weight (kDa)

6.97

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 17 - 56 2e-11 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 310
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 3 cut(s) 46, 104, 369
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AluBI AGCT 2 cut(s) 110, 236
AluI AGCT 2 cut(s) 110, 236
Alw26I GTCTC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 160, 278
BbvI GCAGC 1 cut(s) 320
BccI CCATC 1 cut(s) 116
BclI TGATCA 1 cut(s) 244
BcoDI GTCTC 1 cut(s) 137
BisI GCNGC 1 cut(s) 334
BlsI GCNGC 1 cut(s) 335
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 2 cut(s) 92, 256
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 2 cut(s) 160, 278
Bsa29I ATCGAT 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 264
BseGI GGATG 2 cut(s) 83, 313
BseNI ACTGG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 264
BsmAI GTCTC 1 cut(s) 137
Bso31I GGTCTC 1 cut(s) 137
Bsp143I GATC 1 cut(s) 244
BspDI ATCGAT 1 cut(s) 264
BspQI GCTCTTC 1 cut(s) 276
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 146
BssMI GATC 1 cut(s) 244
Bst6I CTCTTC 2 cut(s) 94, 276
BstC8I GCNNGC 1 cut(s) 238
BstDEI CTNAG 2 cut(s) 179, 278
BstF5I GGATG 2 cut(s) 83, 313
BstKTI GATC 1 cut(s) 247
BstMAI GTCTC 1 cut(s) 137
BstMBI GATC 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 394
BstV1I GCAGC 1 cut(s) 320
BstV2I GAAGAC 2 cut(s) 160, 278
Bsu15I ATCGAT 1 cut(s) 264
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 2 cut(s) 83, 313
Cac8I GCNNGC 1 cut(s) 238
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 309
CviAII CATG 2 cut(s) 27, 391
CviJI RGCY 3 cut(s) 110, 163, 236
CviKI_1 RGCY 3 cut(s) 110, 163, 236
CviQI GTAC 1 cut(s) 309
DdeI CTNAG 2 cut(s) 179, 278
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eam1104I CTCTTC 2 cut(s) 94, 276
EarI CTCTTC 2 cut(s) 94, 276
Eco31I GGTCTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 148
EcoRV GATATC 1 cut(s) 148
FaeI CATG 2 cut(s) 30, 394
FalI AAGNNNNNCTT 2 cut(s) 152, 184
FatI CATG 2 cut(s) 26, 390
FbaI TGATCA 1 cut(s) 244
Fnu4HI GCNGC 1 cut(s) 334
FokI GGATG 2 cut(s) 70, 300
Fsp4HI GCNGC 1 cut(s) 334
GluI GCNGC 1 cut(s) 334
Hin1II CATG 2 cut(s) 30, 394
HinfI GANTC 1 cut(s) 13
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 117, 355
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 98, 203, 410
HpyCH4V TGCA 2 cut(s) 83, 336
HpyF3I CTNAG 2 cut(s) 179, 278
Hsp92II CATG 2 cut(s) 30, 394
Ksp22I TGATCA 1 cut(s) 244
Kzo9I GATC 1 cut(s) 244
LguI GCTCTTC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 107
LpnPI CCDG 4 cut(s) 127, 177, 219, 222
Lsp1109I GCAGC 1 cut(s) 320
LweI GCATC 2 cut(s) 92, 256
MaeIII GTNAC 1 cut(s) 293
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 6 cut(s) 81, 142, 160, 225, 263, 278
MluCI AATT 1 cut(s) 318
MnlI CCTC 3 cut(s) 97, 275, 414
MseI TTAA 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 384
NdeII GATC 1 cut(s) 244
NlaIII CATG 2 cut(s) 30, 394
NmeAIII GCCGAG 1 cut(s) 84
NspI RCATGY 1 cut(s) 394
PciI ACATGT 1 cut(s) 390
PciSI GCTCTTC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 335
PscI ACATGT 1 cut(s) 390
RsaI GTAC 1 cut(s) 310
RsaNI GTAC 1 cut(s) 309
RseI CAYNNNNRTG 1 cut(s) 384
SapI GCTCTTC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 1 cut(s) 334
Sau3AI GATC 1 cut(s) 244
SetI ASST 4 cut(s) 112, 133, 238, 286
SfaNI GCATC 2 cut(s) 92, 256
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 1 cut(s) 318
TaqI TCGA 2 cut(s) 11, 264
TasI AATT 1 cut(s) 318
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TseI GCWGC 1 cut(s) 333
TspDTI ATGAA 2 cut(s) 216, 250
XceI RCATGY 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.