Belongs to the DOCK family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 4512 |
| AarI | CACCTGC | 1 cut(s) | 766 |
| Acc36I | ACCTGC | 4 cut(s) | 766, 3100, 3430, 4786 |
| AccB1I | GGYRCC | 2 cut(s) | 297, 3882 |
| AccB7I | CCANNNNNTGG | 3 cut(s) | 48, 521, 1220 |
| AccBSI | CCGCTC | 1 cut(s) | 3353 |
| AccI | GTMKAC | 1 cut(s) | 4683 |
| AccII | CGCG | 3 cut(s) | 24, 1709, 4930 |
| AccIII | TCCGGA | 1 cut(s) | 4783 |
| AclI | AACGTT | 1 cut(s) | 2476 |
| AclWI | GGATC | 9 cut(s) | 338, 667, 1046, 1059, 1300, 2728, 2766, 4847, 5190 |
| AcoI | YGGCCR | 4 cut(s) | 36, 83, 148, 4275 |
| AcvI | CACGTG | 1 cut(s) | 4697 |
| AcyI | GRCGYC | 2 cut(s) | 298, 3883 |
| AfaI | GTAC | 8 cut(s) | 289, 526, 744, 1354, 3235, 3828, 4427, 5379 |
| AflII | CTTAAG | 1 cut(s) | 1855 |
| AflIII | ACRYGT | 1 cut(s) | 1707 |
| AgeI | ACCGGT | 2 cut(s) | 739, 1319 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3218 |
| AhlI | ACTAGT | 1 cut(s) | 505 |
| AjiI | CACGTC | 2 cut(s) | 4018, 5195 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 2730, 2762, 2865, 2897, 5097, 5129 |
| AleI | CACNNNNGTG | 3 cut(s) | 1553, 4311, 4476 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 4059, 4091 |
| Alw21I | GWGCWC | 6 cut(s) | 1945, 2472, 3084, 3405, 4318, 5270 |
| Alw26I | GTCTC | 5 cut(s) | 212, 789, 1400, 2691, 5201 |
| Alw44I | GTGCAC | 3 cut(s) | 1941, 2468, 5266 |
| AlwI | GGATC | 9 cut(s) | 338, 667, 1046, 1059, 1300, 2728, 2766, 4847, 5190 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 860, 4226 |
| Ama87I | CYCGRG | 1 cut(s) | 2112 |
| Aor13HI | TCCGGA | 1 cut(s) | 4783 |
| ApaLI | GTGCAC | 3 cut(s) | 1941, 2468, 5266 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1914, 1946 |
| AsiGI | ACCGGT | 2 cut(s) | 739, 1319 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 1442, 1841, 2394, 4467, 4900 |
| AspLEI | GCGC | 6 cut(s) | 300, 1854, 3620, 3885, 4930, 5338 |
| AspS9I | GGNCC | 8 cut(s) | 167, 200, 1232, 1322, 2279, 3143, 3786, 4005 |
| AsuC2I | CCSGG | 2 cut(s) | 2113, 2114 |
| AsuHPI | GGTGA | 6 cut(s) | 2399, 3149, 3884, 4658, 4724, 5297 |
| AsuII | TTCGAA | 1 cut(s) | 1933 |
| AvaI | CYCGRG | 1 cut(s) | 2112 |
| AvaII | GGWCC | 5 cut(s) | 167, 1232, 1322, 2279, 4005 |
| AxyI | CCTNAGG | 2 cut(s) | 292, 1236 |
| BaeGI | GKGCMC | 3 cut(s) | 1945, 2472, 5270 |
| BalI | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| BamHI | GGATCC | 1 cut(s) | 1051 |
| BanI | GGYRCC | 2 cut(s) | 297, 3882 |
| BanII | GRGCYC | 4 cut(s) | 3405, 3732, 3929, 4085 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 4818, 4850 |
| BbrPI | CACGTG | 1 cut(s) | 4697 |
| BbsI | GAAGAC | 7 cut(s) | 659, 2397, 3583, 3595, 4031, 5004, 5409 |
| Bbv12I | GWGCWC | 6 cut(s) | 1945, 2472, 3084, 3405, 4318, 5270 |
| BbvCI | CCTCAGC | 1 cut(s) | 4374 |
| BceAI | ACGGC | 4 cut(s) | 157, 3896, 4403, 5151 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 5284, 5318 |
| BciVI | GTATCC | 3 cut(s) | 1518, 1697, 2261 |
| BclI | TGATCA | 1 cut(s) | 2665 |
| BcnI | CCSGG | 2 cut(s) | 2113, 2114 |
| BcoDI | GTCTC | 5 cut(s) | 212, 789, 1400, 2691, 5201 |
| BcuI | ACTAGT | 1 cut(s) | 505 |
| BfoI | RGCGCY | 3 cut(s) | 301, 3886, 5339 |
| BfrI | CTTAAG | 1 cut(s) | 1855 |
| BfuAI | ACCTGC | 4 cut(s) | 766, 3100, 3430, 4786 |
| BfuI | GTATCC | 3 cut(s) | 1518, 1697, 2261 |
| BglII | AGATCT | 2 cut(s) | 465, 4159 |
| BlpI | GCTNAGC | 2 cut(s) | 5, 4538 |
| Bme18I | GGWCC | 5 cut(s) | 167, 1232, 1322, 2279, 4005 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3218 |
| BmeT110I | CYCGRG | 1 cut(s) | 2112 |
| BmgBI | CACGTC | 2 cut(s) | 4018, 5195 |
| BmgT120I | GGNCC | 8 cut(s) | 167, 200, 1232, 1322, 2279, 3143, 3786, 4005 |
| BmrI | ACTGGG | 4 cut(s) | 133, 2501, 3735, 3924 |
| BmuI | ACTGGG | 4 cut(s) | 133, 2501, 3735, 3924 |
| BpiI | GAAGAC | 7 cut(s) | 659, 2397, 3583, 3595, 4031, 5004, 5409 |
| BplI | GAGNNNNNCTC | 8 cut(s) | 2055, 2087, 3298, 3330, 3300, 3332, 4340, 4372 |
| BpmI | CTGGAG | 6 cut(s) | 1985, 2057, 3716, 3920, 4842, 5183 |
| Bpu10I | CCTNAGC | 2 cut(s) | 3321, 4374 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 5, 4538 |
| Bpu14I | TTCGAA | 1 cut(s) | 1933 |
| BpuEI | CTTGAG | 9 cut(s) | 2342, 3213, 3325, 3339, 3439, 4440, 4604, 4968, 5108 |
| BpuMI | CCSGG | 2 cut(s) | 2113, 2114 |
| BsaAI | YACGTR | 3 cut(s) | 4322, 4697, 5147 |
| BsaBI | GATNNNNATC | 4 cut(s) | 2670, 4197, 4761, 5440 |
| BsaHI | GRCGYC | 2 cut(s) | 298, 3883 |
| BsaI | GGTCTC | 2 cut(s) | 789, 2691 |
| BsaWI | WCCGGW | 4 cut(s) | 739, 1319, 4783, 5282 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1590, 1620, 4059, 4089 |
| Bse118I | RCCGGY | 2 cut(s) | 739, 1319 |
| Bse21I | CCTNAGG | 2 cut(s) | 292, 1236 |
| Bse3DI | GCAATG | 7 cut(s) | 1509, 1754, 1998, 2062, 2310, 3578, 5328 |
| Bse8I | GATNNNNATC | 4 cut(s) | 2670, 4197, 4761, 5440 |
| BseAI | TCCGGA | 1 cut(s) | 4783 |
| BseJI | GATNNNNATC | 4 cut(s) | 2670, 4197, 4761, 5440 |
| BseMI | GCAATG | 7 cut(s) | 1509, 1754, 1998, 2062, 2310, 3578, 5328 |
| BseRI | GAGGAG | 3 cut(s) | 357, 2030, 3760 |
| BseSI | GKGCMC | 3 cut(s) | 1945, 2472, 5270 |
| BseYI | CCCAGC | 1 cut(s) | 5044 |
| BsgI | GTGCAG | 3 cut(s) | 125, 873, 2138 |
| Bsh1236I | CGCG | 3 cut(s) | 24, 1709, 4930 |
| BshNI | GGYRCC | 2 cut(s) | 297, 3882 |
| BshTI | ACCGGT | 2 cut(s) | 739, 1319 |
| BsiHKAI | GWGCWC | 6 cut(s) | 1945, 2472, 3084, 3405, 4318, 5270 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2112 |
| BsiSI | CCGG | 6 cut(s) | 35, 740, 1320, 2113, 4784, 5283 |
| BslFI | GGGAC | 3 cut(s) | 469, 2502, 4139 |
| BsmAI | GTCTC | 5 cut(s) | 212, 789, 1400, 2691, 5201 |
| BsmBI | CGTCTC | 1 cut(s) | 5201 |
| BsmFI | GGGAC | 3 cut(s) | 469, 2502, 4139 |
| BsmI | GAATGC | 3 cut(s) | 3068, 4485, 5273 |
| Bso31I | GGTCTC | 2 cut(s) | 789, 2691 |
| BsoBI | CYCGRG | 1 cut(s) | 2112 |
| Bsp119I | TTCGAA | 1 cut(s) | 1933 |
| Bsp1286I | GDGCHC | 9 cut(s) | 1945, 2472, 3084, 3405, 3732, 3929, 4085, 4318, 5270 |
| Bsp13I | TCCGGA | 1 cut(s) | 4783 |
| Bsp1407I | TGTACA | 2 cut(s) | 3826, 5377 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 5, 4538 |
| Bsp19I | CCATGG | 2 cut(s) | 2094, 3254 |
| BspEI | TCCGGA | 1 cut(s) | 4783 |
| BspFNI | CGCG | 3 cut(s) | 24, 1709, 4930 |
| BspHI | TCATGA | 5 cut(s) | 916, 2668, 2761, 3534, 3741 |
| BspMAI | CTGCAG | 4 cut(s) | 777, 3441, 4117, 4372 |
| BspMI | ACCTGC | 4 cut(s) | 766, 3100, 3430, 4786 |
| BspPI | GGATC | 9 cut(s) | 338, 667, 1046, 1059, 1300, 2728, 2766, 4847, 5190 |
| BspQI | GCTCTTC | 1 cut(s) | 5154 |
| BspT104I | TTCGAA | 1 cut(s) | 1933 |
| BspT107I | GGYRCC | 2 cut(s) | 297, 3882 |
| BspTI | CTTAAG | 1 cut(s) | 1855 |
| BspTNI | GGTCTC | 2 cut(s) | 789, 2691 |
| BsrBI | CCGCTC | 1 cut(s) | 3353 |
| BsrDI | GCAATG | 7 cut(s) | 1509, 1754, 1998, 2062, 2310, 3578, 5328 |
| BsrFI | RCCGGY | 2 cut(s) | 739, 1319 |
| BsrGI | TGTACA | 2 cut(s) | 3826, 5377 |
| BssAI | RCCGGY | 2 cut(s) | 739, 1319 |
| BssNAI | GTATAC | 1 cut(s) | 4684 |
| BssNI | GRCGYC | 2 cut(s) | 298, 3883 |
| BssT1I | CCWWGG | 5 cut(s) | 252, 1511, 1946, 2094, 3254 |
| Bst1107I | GTATAC | 1 cut(s) | 4684 |
| Bst6I | CTCTTC | 7 cut(s) | 547, 1935, 2369, 2728, 3858, 5003, 5154 |
| BstACI | GRCGYC | 2 cut(s) | 298, 3883 |
| BstAFI | CTTAAG | 1 cut(s) | 1855 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 772, 4226 |
| BstAUI | TGTACA | 2 cut(s) | 3826, 5377 |
| BstBAI | YACGTR | 3 cut(s) | 4322, 4697, 5147 |
| BstBI | TTCGAA | 1 cut(s) | 1933 |
| BstDSI | CCRYGG | 3 cut(s) | 625, 2094, 3254 |
| BstEII | GGTNACC | 1 cut(s) | 3248 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1080, 4586 |
| BstFNI | CGCG | 3 cut(s) | 24, 1709, 4930 |
| BstH2I | RGCGCY | 3 cut(s) | 301, 3886, 5339 |
| BstHHI | GCGC | 6 cut(s) | 300, 1854, 3620, 3885, 4930, 5338 |
| BstMAI | GTCTC | 5 cut(s) | 212, 789, 1400, 2691, 5201 |
| BstNSI | RCATGY | 6 cut(s) | 692, 1581, 1733, 2038, 3106, 4073 |
| BstPI | GGTNACC | 1 cut(s) | 3248 |
| BstSLI | GKGCMC | 3 cut(s) | 1945, 2472, 5270 |
| BstSNI | TACGTA | 2 cut(s) | 4322, 5147 |
| BstUI | CGCG | 3 cut(s) | 24, 1709, 4930 |
| BstV2I | GAAGAC | 7 cut(s) | 659, 2397, 3583, 3595, 4031, 5004, 5409 |
| BstX2I | RGATCY | 3 cut(s) | 465, 1051, 4159 |
| BstYI | RGATCY | 3 cut(s) | 465, 1051, 4159 |
| BstZ17I | GTATAC | 1 cut(s) | 4684 |
| Bsu36I | CCTNAGG | 2 cut(s) | 292, 1236 |
| BsuI | GTATCC | 3 cut(s) | 1518, 1697, 2261 |
| BtgI | CCRYGG | 3 cut(s) | 625, 2094, 3254 |
| BtgZI | GCGATG | 2 cut(s) | 3010, 3475 |
| BtrI | CACGTC | 2 cut(s) | 4018, 5195 |
| BtsI | GCAGTG | 4 cut(s) | 86, 113, 3693, 5268 |
| BveI | ACCTGC | 4 cut(s) | 766, 3100, 3430, 4786 |
| CaiI | CAGNNNCTG | 2 cut(s) | 860, 4226 |
| CciI | TCATGA | 5 cut(s) | 916, 2668, 2761, 3534, 3741 |
| CfoI | GCGC | 6 cut(s) | 300, 1854, 3620, 3885, 4930, 5338 |
| Cfr10I | RCCGGY | 2 cut(s) | 739, 1319 |
| Cfr13I | GGNCC | 8 cut(s) | 167, 200, 1232, 1322, 2279, 3143, 3786, 4005 |
| Cfr9I | CCCGGG | 1 cut(s) | 2112 |
| CseI | GACGC | 3 cut(s) | 653, 1698, 3508 |
| Csp6I | GTAC | 8 cut(s) | 288, 525, 743, 1353, 3234, 3827, 4426, 5378 |
| CspAI | ACCGGT | 2 cut(s) | 739, 1319 |
| CviQI | GTAC | 8 cut(s) | 288, 525, 743, 1353, 3234, 3827, 4426, 5378 |
| DinI | GGCGCC | 2 cut(s) | 299, 3884 |
| DraI | TTTAAA | 2 cut(s) | 1983, 2511 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3218 |
| EaeI | YGGCCR | 4 cut(s) | 36, 83, 148, 4275 |
| Eam1104I | CTCTTC | 7 cut(s) | 547, 1935, 2369, 2728, 3858, 5003, 5154 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3218 |
| EarI | CTCTTC | 7 cut(s) | 547, 1935, 2369, 2728, 3858, 5003, 5154 |
| EciI | GGCGGA | 5 cut(s) | 159, 272, 2076, 2388, 5029 |
| Ecl136II | GAGCTC | 1 cut(s) | 3403 |
| Eco105I | TACGTA | 2 cut(s) | 4322, 5147 |
| Eco130I | CCWWGG | 5 cut(s) | 252, 1511, 1946, 2094, 3254 |
| Eco24I | GRGCYC | 4 cut(s) | 3405, 3732, 3929, 4085 |
| Eco31I | GGTCTC | 2 cut(s) | 789, 2691 |
| Eco47I | GGWCC | 5 cut(s) | 167, 1232, 1322, 2279, 4005 |
| Eco53kI | GAGCTC | 1 cut(s) | 3403 |
| Eco72I | CACGTG | 1 cut(s) | 4697 |
| Eco81I | CCTNAGG | 2 cut(s) | 292, 1236 |
| Eco88I | CYCGRG | 1 cut(s) | 2112 |
| Eco91I | GGTNACC | 1 cut(s) | 3248 |
| EcoICRI | GAGCTC | 1 cut(s) | 3403 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1080, 4586 |
| EcoO109I | RGGNCCY | 1 cut(s) | 200 |
| EcoO65I | GGTNACC | 1 cut(s) | 3248 |
| EcoRI | GAATTC | 3 cut(s) | 1258, 5214, 5411 |
| EcoT14I | CCWWGG | 5 cut(s) | 252, 1511, 1946, 2094, 3254 |
| EcoT22I | ATGCAT | 1 cut(s) | 2435 |
| EcoT38I | GRGCYC | 4 cut(s) | 3405, 3732, 3929, 4085 |
| EgeI | GGCGCC | 2 cut(s) | 299, 3884 |
| EheI | GGCGCC | 2 cut(s) | 299, 3884 |
| ErhI | CCWWGG | 5 cut(s) | 252, 1511, 1946, 2094, 3254 |
| Esp3I | CGTCTC | 1 cut(s) | 5201 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 1826, 1858, 1930, 1962, 3355, 3387, 4449, 4481 |
| FaqI | GGGAC | 3 cut(s) | 469, 2502, 4139 |
| FauI | CCCGC | 3 cut(s) | 698, 2772, 4515 |
| FauNDI | CATATG | 1 cut(s) | 4528 |
| FbaI | TGATCA | 1 cut(s) | 2665 |
| FblI | GTMKAC | 1 cut(s) | 4683 |
| FriOI | GRGCYC | 4 cut(s) | 3405, 3732, 3929, 4085 |
| GlaI | GCGC | 6 cut(s) | 299, 1853, 3619, 3884, 4929, 5337 |
| GsaI | CCCAGC | 1 cut(s) | 5048 |
| GsuI | CTGGAG | 6 cut(s) | 1985, 2057, 3716, 3920, 4842, 5183 |
| HaeII | RGCGCY | 3 cut(s) | 301, 3886, 5339 |
| HapII | CCGG | 6 cut(s) | 35, 740, 1320, 2113, 4784, 5283 |
| HgaI | GACGC | 3 cut(s) | 653, 1698, 3508 |
| HhaI | GCGC | 6 cut(s) | 300, 1854, 3620, 3885, 4930, 5338 |
| Hin1I | GRCGYC | 2 cut(s) | 298, 3883 |
| Hin6I | GCGC | 6 cut(s) | 298, 1852, 3618, 3883, 4928, 5336 |
| HinP1I | GCGC | 6 cut(s) | 298, 1852, 3618, 3883, 4928, 5336 |
| HincII | GTYRAC | 6 cut(s) | 1129, 2164, 2229, 4411, 5056, 5245 |
| HindII | GTYRAC | 6 cut(s) | 1129, 2164, 2229, 4411, 5056, 5245 |
| HindIII | AAGCTT | 3 cut(s) | 1338, 4120, 4130 |
| HpaI | GTTAAC | 1 cut(s) | 5056 |
| HpaII | CCGG | 6 cut(s) | 35, 740, 1320, 2113, 4784, 5283 |
| HphI | GGTGA | 6 cut(s) | 2399, 3149, 3884, 4658, 4724, 5297 |
| Hpy99I | CGWCG | 3 cut(s) | 178, 992, 4915 |
| HpyCH4IV | ACGT | 7 cut(s) | 1199, 2476, 4017, 4321, 4696, 5146, 5194 |
| HpySE526I | ACGT | 7 cut(s) | 1199, 2476, 4017, 4321, 4696, 5146, 5194 |
| Hsp92I | GRCGYC | 2 cut(s) | 298, 3883 |
| HspAI | GCGC | 6 cut(s) | 298, 1852, 3618, 3883, 4928, 5336 |
| KasI | GGCGCC | 2 cut(s) | 297, 3882 |
| Kpn2I | TCCGGA | 1 cut(s) | 4783 |
| Ksp22I | TGATCA | 1 cut(s) | 2665 |
| KspAI | GTTAAC | 1 cut(s) | 5056 |
| LguI | GCTCTTC | 1 cut(s) | 5154 |
| MaeII | ACGT | 7 cut(s) | 1199, 2476, 4017, 4321, 4696, 5146, 5194 |
| MbiI | CCGCTC | 1 cut(s) | 3353 |
| MfeI | CAATTG | 1 cut(s) | 2408 |
| MflI | RGATCY | 3 cut(s) | 465, 1051, 4159 |
| MhlI | GDGCHC | 9 cut(s) | 1945, 2472, 3084, 3405, 3732, 3929, 4085, 4318, 5270 |
| MlsI | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| MluI | ACGCGT | 1 cut(s) | 1707 |
| MluNI | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| Mly113I | GGCGCC | 2 cut(s) | 298, 3883 |
| MlyI | GAGTC | 9 cut(s) | 815, 1034, 1163, 2135, 2355, 3829, 4406, 4754, 4941 |
| MmeI | TCCRAC | 3 cut(s) | 1656, 1794, 2257 |
| Mox20I | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| Mph1103I | ATGCAT | 1 cut(s) | 2435 |
| MroI | TCCGGA | 1 cut(s) | 4783 |
| MroXI | GAANNNNTTC | 5 cut(s) | 1442, 1841, 2394, 4467, 4900 |
| MscI | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| Msp20I | TGGCCA | 3 cut(s) | 85, 150, 4277 |
| MspA1I | CMGCKG | 3 cut(s) | 4112, 4373, 4537 |
| MspCI | CTTAAG | 1 cut(s) | 1855 |
| MspI | CCGG | 6 cut(s) | 35, 740, 1320, 2113, 4784, 5283 |
| MunI | CAATTG | 1 cut(s) | 2408 |
| Mva1269I | GAATGC | 3 cut(s) | 3068, 4485, 5273 |
| MvnI | CGCG | 3 cut(s) | 24, 1709, 4930 |
| NarI | GGCGCC | 2 cut(s) | 298, 3883 |
| NciI | CCSGG | 2 cut(s) | 2113, 2114 |
| NcoI | CCATGG | 2 cut(s) | 2094, 3254 |
| NdeI | CATATG | 1 cut(s) | 4528 |
| NsiI | ATGCAT | 1 cut(s) | 2435 |
| NspI | RCATGY | 6 cut(s) | 692, 1581, 1733, 2038, 3106, 4073 |
| NspV | TTCGAA | 1 cut(s) | 1933 |
| OliI | CACNNNNGTG | 3 cut(s) | 1553, 4311, 4476 |
| PaeI | GCATGC | 3 cut(s) | 692, 1581, 3106 |
| PagI | TCATGA | 5 cut(s) | 916, 2668, 2761, 3534, 3741 |
| PaqCI | CACCTGC | 1 cut(s) | 766 |
| PciSI | GCTCTTC | 1 cut(s) | 5154 |
| PctI | GAATGC | 3 cut(s) | 3068, 4485, 5273 |
| PdmI | GAANNNNTTC | 5 cut(s) | 1442, 1841, 2394, 4467, 4900 |
| PflMI | CCANNNNNTGG | 3 cut(s) | 48, 521, 1220 |
| PfoI | TCCNGGA | 1 cut(s) | 3335 |
| PinAI | ACCGGT | 2 cut(s) | 739, 1319 |
| PleI | GAGTC | 9 cut(s) | 814, 1034, 1163, 2134, 2355, 3829, 4406, 4753, 4940 |
| PluTI | GGCGCC | 2 cut(s) | 301, 3886 |
| PmaCI | CACGTG | 1 cut(s) | 4697 |
| PmlI | CACGTG | 1 cut(s) | 4697 |
| PpsI | GAGTC | 9 cut(s) | 814, 1034, 1163, 2134, 2355, 3829, 4406, 4753, 4940 |
| Ppu21I | YACGTR | 3 cut(s) | 4322, 4697, 5147 |
| PsiI | TTATAA | 1 cut(s) | 4512 |
| Psp124BI | GAGCTC | 1 cut(s) | 3405 |
| Psp1406I | AACGTT | 1 cut(s) | 2476 |
| PspCI | CACGTG | 1 cut(s) | 4697 |
| PspEI | GGTNACC | 1 cut(s) | 3248 |
| PspFI | CCCAGC | 1 cut(s) | 5044 |
| PspPI | GGNCC | 8 cut(s) | 167, 200, 1232, 1322, 2279, 3143, 3786, 4005 |
| PstI | CTGCAG | 4 cut(s) | 777, 3441, 4117, 4372 |
| PstNI | CAGNNNCTG | 2 cut(s) | 860, 4226 |
| PsuI | RGATCY | 3 cut(s) | 465, 1051, 4159 |
| PvuII | CAGCTG | 3 cut(s) | 4112, 4373, 4537 |
| RsaI | GTAC | 8 cut(s) | 289, 526, 744, 1354, 3235, 3828, 4427, 5379 |
| RsaNI | GTAC | 8 cut(s) | 288, 525, 743, 1353, 3234, 3827, 4426, 5378 |
| SacI | GAGCTC | 1 cut(s) | 3405 |
| SapI | GCTCTTC | 1 cut(s) | 5154 |
| Sau96I | GGNCC | 8 cut(s) | 167, 200, 1232, 1322, 2279, 3143, 3786, 4005 |
| SchI | GAGTC | 9 cut(s) | 815, 1034, 1163, 2135, 2355, 3829, 4406, 4754, 4941 |
| SduI | GDGCHC | 9 cut(s) | 1945, 2472, 3084, 3405, 3732, 3929, 4085, 4318, 5270 |
| SfoI | GGCGCC | 2 cut(s) | 299, 3884 |
| SfuI | TTCGAA | 1 cut(s) | 1933 |
| SinI | GGWCC | 5 cut(s) | 167, 1232, 1322, 2279, 4005 |
| SmaI | CCCGGG | 1 cut(s) | 2114 |
| SnaBI | TACGTA | 2 cut(s) | 4322, 5147 |
| SpeI | ACTAGT | 1 cut(s) | 505 |
| SphI | GCATGC | 3 cut(s) | 692, 1581, 3106 |
| SspDI | GGCGCC | 2 cut(s) | 297, 3882 |
| SspI | AATATT | 2 cut(s) | 610, 3384 |
| SstI | GAGCTC | 1 cut(s) | 3405 |
| StyI | CCWWGG | 5 cut(s) | 252, 1511, 1946, 2094, 3254 |
| TaiI | ACGT | 7 cut(s) | 1202, 2479, 4020, 4324, 4699, 5149, 5197 |
| TatI | WGTACW | 2 cut(s) | 3826, 5377 |
| TauI | GCSGC | 4 cut(s) | 24, 2792, 3479, 5336 |
| TspGWI | ACGGA | 5 cut(s) | 323, 976, 1340, 3519, 5363 |
| TspMI | CCCGGG | 1 cut(s) | 2112 |
| Van91I | CCANNNNNTGG | 3 cut(s) | 48, 521, 1220 |
| Vha464I | CTTAAG | 1 cut(s) | 1855 |
| VneI | GTGCAC | 3 cut(s) | 1941, 2468, 5266 |
| VpaK11BI | GGWCC | 5 cut(s) | 167, 1232, 1322, 2279, 4005 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1080, 4586 |
| XbaI | TCTAGA | 1 cut(s) | 2902 |
| XceI | RCATGY | 6 cut(s) | 692, 1581, 1733, 2038, 3106, 4073 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 393 |
| XmaI | CCCGGG | 1 cut(s) | 2112 |
| XmiI | GTMKAC | 1 cut(s) | 4683 |
| XmnI | GAANNNNTTC | 5 cut(s) | 1442, 1841, 2394, 4467, 4900 |
| Zsp2I | ATGCAT | 1 cut(s) | 2435 |