Rorug05G0083100

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000922
Physical Location & Seq
Reverse (-)
7175620 .. 7180458
4839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0083100.1

Sequence Viewer

Length: 3111 bp
ATGGAGAGAGCTCAGTTCTTCCTCTTGTGCATGACATTGTTGCTTTGCATGGCCAGAGCACGAACCAATTTGACGACAGACCAGTCTGCTCTTCTTGCTCTCAAAGCTAATGTCACTAGTGACCCTCAAGACTTGTTGTCCAACTGGTCTACCAATACCTCCGTTTGCAACTGGGTTGGGGTTACTTGTGGTCCTGTCCATCTTAGAGTCGCTGCCTTGAATCTCTCTTATTTTGGGCTCACAGGTAGAATTCCTCCGGAGCTAGGAAACCTTTCATTTCTTGTTGTGCTACGCTTGAGAAACAACAGTTTTAATGGCACTTTGCCTGTGGAATTAGCTGGTTCGCGCCGATTGAAGTTGGTTAACTTCAAATACAATAACTTTATGGGCGAGATTCCATCATGGTTTGGGTCCTTATCCAGTCTTAAAGCCTTGAATTTGTATGGGAATCGGTTCACAGGATCCATACCTGCTGCTATCTTCAACTTATCTGCTCTGCAAGTATTTGATCTGAGAAATAACCAGCTATCAGGTAGCATACCGAGAGAAATCGGGAACTTAACAATGCTGAAGCATTTATACCTTGACAATAACAATTTCCAAGAAATTCCGGATGAGATCGGCAGTTTACATCAGCTGGATTTGCTGTCTATGCAATACAATGGCCTAAAAGGGCGTGTTCCTTTGGTTTTCTTCAACATGTCTTCTTTGACTATTTTGGGCCTTTCAGGAAACAGTTTCAGTGGTCGTCTTCCTGACAATATATGTCAGAATCTTCCTAGTATTCAAAGGTTGTACTTTTCTATCAACCAGTTTGATGGTCCACTTCCATCCCAATTATGGCAATGCAAACAGCTTCTTGTATTGTCATTATCTGTTAACAATTTCAGTGGAAGTATACCCAGAAATATTGGCAACTTGACTAAGTTACAGAAGATTTATCTAGACTTCAACAATTTGACAGGTACTATTCCGCATGAGATTGGCTATCTTCAAAATTTAGAGATATTGTCACTCCTTGCTAACTCATTTTCTGGGTCCATTCCTACCACACTCTGTGCCTTACCAGAACTCATGTCTCTTAACTTGGGCAAGAATAAGTTAACCATTGATACTTCTACTCCAGAAGCCAACATTTTCTCTTGTTTGGCCAATCTTAAAAATTTGTGGAGATTAAATTTGTCAAGCAATCCATTATATGCCACGCTTCCTGTTTCCCTCAAGAACTTCTCTACATCCATTCAATATCTGGATATGAGCAGTTGCAACATAGGGGGTAACATTCCCAACGGTATTGGCAACTTGAGCAACTTGACAGTCTTAGACTTGGGATACAATCAACTGAGTGGATCAATTCCAACTACAATGGGGAGACTACAGAACCTCCAAGGTTTGTACTTGACTGATAACAAATTGCAAGGATTTATTCCTGATGAACTTTGTCAACTAAACAATCTAGTTGACTTAGTTTTGGGTGGTAATCAACTTTCTGGTTCTGTACCTTCCTGCTTGGGTAATCTAACAGCTCTAAGAACTCTGTCATTAGGGTCCAATTCTTTAACTTCAACAGTACCATCTGCAGTGTGGGGACTTACATATATCTTACACATAAACTTGTCATCCAATTCTCTACTTGGTCCTATCTCAGCAGATGTTGGAAATTTAAGAGTTGTCACAAATATAGATTTGTCAAGCAACAACTTTTCAGGTACTATACCAAGCAACATTGGGTGGCTCGAAAACTTGGTTAATCTCTCCTTGGCAAATAACAATCTCGAAGGCCCAATTCCCAGTTCATTTGGCAACTTAGTAAGCCTGGAGCTCTTGGATCTATCCAGAAACAATCTATCTGGAATCATTCCAGAGTCTTTAGAAGCGCTCTTGCATCTCAGGTATCTGAATTTGTCTTTCAACAAACTCCATGGAGAAATTCCAACAGGTGGACCTTTCAAAAACTTCTCTGTTCAGTCATTTCTCTCAAATGATGGACTATGTGGTGCATCACAGCTTGATTTCCTACCATGCAGAACCCCTGTACAATATTCAAGGACAGCTAGTTCATCTATGTTGAAATATATTATTCCAGGGATGTTGGCAGCAATGCTTGTATTGACCTTCATATGGTTGTTCACACTACGCAAAAAAGGGGATGCAGAGGTTGCAACAGAGCATACCTTAATACCTGAACTTCTTCGGAAAAAAGTTACATACCAAGAAATTCTAAGTGCTACAAATGGCTTCAATGAAACCAACTTACTTGGCTCTGGGGGATTTGGCTCAGTGTATAAAGGAATACTTTCAAATGGGATAGCGGTTGCTATAAAGGTGTTCAATTTACAGCACGAAGATGCTTTCAAAAAATTTGATGTTGAAAGTGAACTGCTAAGCAATGTTTGTCATCACAATCTTATCAAAATCATCGGTAGTTGCAACCAAAGTCATTTCAAAGCCTTGGTACTTGACTACATGCCTAATGGGAGCCTCGAGAGGTTGCTGTATTCTGAAGAATACTGTTCTTTGAAAATCCTAGTGAGGTTGAACATAATGATAGATGTTGCATCGGCGTTGGAATACCTTCATCATGGTTATTCAATACCAATTGTCCATTGTGATTTGAAGCCAAGCAATATACTACTAGACAATGATATGGTTGCACATGTTGCTGATTTTGGCATTGCAAAACTCTTGGGTGGAGGGGATTCTGTGACCGAAACCATGACACTAGCTACAGTTGGGTATATGGCTCCAGAATATGGAATAGAAGGAATAATTACCAGAAGAGGGGATGTGTACAGTTTTGGTATAGTACTAATGGAGACTTTCACAAAAAAGAAGCCTACAGATGAGATATTTGTTGGGGAAATGAGTTTAAAGCGATGGGTTGCAGATTCGTTACTTTCAGATGCAGTACTTGAAGTTGTGGATGGCAGTTTACTTGGGAAAGAGGAAGATCATGATTTTGTGAATAAGAGGGATTGTTTATCTTCCATTATGAGGTTGGCACTAGATTGTTCTGCAGAATCAGTTGAAGAGAGGATAAGTATGCAAGGTGCTGCAGTCACACTCCATAAAATCAAGAACAATTTTTTGAAGGACATTGGAGGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

1036

Amino Acids

113.37

Weight (kDa)

6.02

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 26 - 63 1e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 119 - 176 1.6e-06 Leucine rich repeat
LRR_8 PF13855 141 - 200 9.2e-09 Leucine rich repeat
LRR_14 PF23598 162 - 266 2e-11 Leucine-rich repeat region
LRR_8 PF13855 190 - 246 2.4e-06 Leucine rich repeat
LRR_14 PF23598 278 - 395 4.6e-09 Leucine-rich repeat region
LRR_14 PF23598 414 - 517 1.3e-10 Leucine-rich repeat region
LRR_8 PF13855 436 - 496 2e-09 Leucine rich repeat
LRR_14 PF23598 546 - 640 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 581 - 640 2.4e-10 Leucine rich repeat
Pkinase PF00069 748 - 1016 2.8e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 751 - 1016 1.3e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 82
Acc36I ACCTGC 1 cut(s) 478
AccB7I CCANNNNNTGG 2 cut(s) 1940, 2753
AccI GTMKAC 2 cut(s) 149, 898
AccII CGCG 1 cut(s) 346
AccIII TCCGGA 2 cut(s) 256, 610
AciI CCGC 2 cut(s) 974, 2312
AclWI GGATC 4 cut(s) 456, 469, 1357, 1836
AcoI YGGCCR 2 cut(s) 51, 1149
AcuI CTGAAG 2 cut(s) 590, 2523
AdeI CACNNNGTG 1 cut(s) 1058
AfeI AGCGCT 1 cut(s) 1878
AfiI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2753, 2781, 2994
AflIII ACRYGT 2 cut(s) 699, 2656
AhdI GACNNNNNGTC 1 cut(s) 136
AhlI ACTAGT 1 cut(s) 116
AjnI CCWGG 2 cut(s) 1815, 2083
AjuI GAANNNNNNNTTGG 2 cut(s) 1777, 1809
AloI GAACNNNNNNTCC 2 cut(s) 2041, 2073
Alw21I GWGCWC 3 cut(s) 13, 61, 1824
Alw26I GTCTC 3 cut(s) 1083, 1366, 2810
AlwI GGATC 4 cut(s) 456, 469, 1357, 1836
Ama87I CYCGRG 1 cut(s) 2483
Aor13HI TCCGGA 2 cut(s) 256, 610
Aor51HI AGCGCT 1 cut(s) 1878
AoxI GGCC 5 cut(s) 51, 664, 721, 1149, 1780
ApeKI GCWGC 4 cut(s) 212, 473, 2096, 3053
ArsI GACNNNNNNTTYG 2 cut(s) 96, 128
Asp700I GAANNNNTTC 5 cut(s) 271, 452, 2190, 2296, 2574
AspLEI GCGC 2 cut(s) 348, 1879
AspS9I GGNCC 9 cut(s) 191, 411, 721, 821, 1038, 1548, 1637, 1781, 1943
AvaI CYCGRG 1 cut(s) 2483
AvaII GGWCC 7 cut(s) 191, 411, 821, 1038, 1548, 1637, 1943
BalI TGGCCA 2 cut(s) 53, 1151
BamHI GGATCC 1 cut(s) 461
BanII GRGCYC 3 cut(s) 13, 240, 1824
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BbsI GAAGAC 2 cut(s) 696, 743
Bbv12I GWGCWC 3 cut(s) 13, 61, 1824
BbvI GCAGC 4 cut(s) 199, 460, 2108, 3040
BccI CCATC 8 cut(s) 207, 406, 812, 838, 1582, 1979, 2871, 2918
BciT130I CCWGG 2 cut(s) 1817, 2085
BciVI GTATCC 1 cut(s) 1325
BcoDI GTCTC 3 cut(s) 1083, 1366, 2810
BcuI ACTAGT 1 cut(s) 116
BfmI CTRYAG 6 cut(s) 1376, 1578, 2727, 2838, 3015, 3054
BfoI RGCGCY 1 cut(s) 1880
BfuAI ACCTGC 1 cut(s) 478
BfuI GTATCC 1 cut(s) 1325
BisI GCNGC 4 cut(s) 213, 474, 2097, 3054
BlpI GCTNAGC 1 cut(s) 2384
BlsI GCNGC 4 cut(s) 214, 475, 2098, 3055
BmcAI AGTACT 2 cut(s) 2808, 2910
Bme1390I CCNGG 2 cut(s) 1817, 2085
Bme18I GGWCC 7 cut(s) 191, 411, 821, 1038, 1548, 1637, 1943
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 1 cut(s) 2483
BmgT120I GGNCC 9 cut(s) 191, 411, 721, 821, 1038, 1548, 1637, 1781, 1943
BmiI GGNNCC 6 cut(s) 412, 463, 1039, 1549, 2480, 2745
BmrFI CCNGG 2 cut(s) 1817, 2085
BmrI ACTGGG 2 cut(s) 181, 1785
BmsI GCATC 6 cut(s) 1894, 2009, 2140, 2338, 2567, 2893
BmuI ACTGGG 2 cut(s) 181, 1785
BpiI GAAGAC 2 cut(s) 696, 743
BpmI CTGGAG 3 cut(s) 1107, 1838, 2730
Bpu1102I GCTNAGC 1 cut(s) 2384
BpuEI CTTGAG 4 cut(s) 111, 316, 1205, 1324
BsaJI CCNNGG 5 cut(s) 1387, 1758, 1921, 2084, 2451
BsaWI WCCGGW 2 cut(s) 256, 610
BsaXI ACNNNNNCTCC 4 cut(s) 1105, 1135, 1368, 1398
Bsc4I CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2753, 2781, 2994
Bse1I ACTGG 6 cut(s) 82, 149, 176, 420, 811, 1791
Bse3DI GCAATG 4 cut(s) 851, 2106, 2395, 2673
BseAI TCCGGA 2 cut(s) 256, 610
BseBI CCWGG 2 cut(s) 1817, 2085
BseDI CCNNGG 5 cut(s) 1387, 1758, 1921, 2084, 2451
BseGI GGATG 8 cut(s) 619, 830, 1235, 1619, 2094, 2155, 2791, 2929
BseLI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2753, 2781, 2994
BseMI GCAATG 4 cut(s) 851, 2106, 2395, 2673
BseMII CTCAG 6 cut(s) 26, 503, 1334, 1659, 1903, 2292
BseNI ACTGG 6 cut(s) 82, 149, 176, 420, 811, 1791
BseXI GCAGC 4 cut(s) 199, 460, 2108, 3040
Bsh1236I CGCG 1 cut(s) 346
BshFI GGCC 5 cut(s) 53, 666, 723, 1151, 1782
BsiHKAI GWGCWC 3 cut(s) 13, 61, 1824
BsiHKCI CYCGRG 1 cut(s) 2483
BsiSI CCGG 2 cut(s) 257, 611
BslFI GGGAC 1 cut(s) 1602
BslI CCNNNNNNNGG 7 cut(s) 263, 840, 1940, 2121, 2753, 2781, 2994
BsmAI GTCTC 3 cut(s) 1083, 1366, 2810
BsmFI GGGAC 1 cut(s) 1602
BsnI GGCC 5 cut(s) 53, 666, 723, 1151, 1782
BsoBI CYCGRG 1 cut(s) 2483
Bsp1286I GDGCHC 4 cut(s) 13, 61, 240, 1824
Bsp13I TCCGGA 2 cut(s) 256, 610
Bsp1407I TGTACA 2 cut(s) 2035, 2790
Bsp143I GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
Bsp1720I GCTNAGC 1 cut(s) 2384
Bsp19I CCATGG 1 cut(s) 1921
BspACI CCGC 2 cut(s) 974, 2312
BspANI GGCC 5 cut(s) 53, 666, 723, 1151, 1782
BspCNI CTCAG 6 cut(s) 25, 504, 1335, 1658, 1902, 2291
BspEI TCCGGA 2 cut(s) 256, 610
BspFNI CGCG 1 cut(s) 346
BspHI TCATGA 1 cut(s) 2953
BspLI GGNNCC 6 cut(s) 412, 463, 1039, 1549, 2480, 2745
BspMAI CTGCAG 3 cut(s) 1582, 3019, 3058
BspMI ACCTGC 1 cut(s) 478
BspPI GGATC 4 cut(s) 456, 469, 1357, 1836
BspQI GCTCTTC 1 cut(s) 96
BsrDI GCAATG 4 cut(s) 851, 2106, 2395, 2673
BsrGI TGTACA 2 cut(s) 2035, 2790
BsrI ACTGG 6 cut(s) 82, 149, 176, 420, 811, 1791
BssECI CCNNGG 5 cut(s) 1387, 1758, 1921, 2084, 2451
BssMI GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
BssNAI GTATAC 1 cut(s) 899
BssT1I CCWWGG 4 cut(s) 1387, 1758, 1921, 2451
Bst1107I GTATAC 1 cut(s) 899
Bst2UI CCWGG 2 cut(s) 1817, 2085
Bst4CI ACNGT 8 cut(s) 308, 737, 1292, 1318, 1570, 2513, 2731, 2795
Bst6I CTCTTC 3 cut(s) 96, 2773, 3024
BstAPI GCANNNNNTGC 2 cut(s) 2159, 2660
BstAUI TGTACA 2 cut(s) 2035, 2790
BstDSI CCRYGG 1 cut(s) 1921
BstF5I GGATG 8 cut(s) 619, 830, 1235, 1619, 2094, 2155, 2791, 2929
BstFNI CGCG 1 cut(s) 346
BstH2I RGCGCY 1 cut(s) 1880
BstHHI GCGC 2 cut(s) 348, 1879
BstKTI GATC 6 cut(s) 464, 511, 621, 1352, 1831, 2953
BstMAI GTCTC 3 cut(s) 1083, 1366, 2810
BstMBI GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
BstMWI GCNNNNNNNGC 7 cut(s) 95, 104, 643, 652, 1305, 2159, 2660
BstNI CCWGG 2 cut(s) 1817, 2085
BstNSI RCATGY 3 cut(s) 703, 2470, 2660
BstSCI CCNGG 2 cut(s) 1815, 2083
BstSFI CTRYAG 6 cut(s) 1376, 1578, 2727, 2838, 3015, 3054
BstUI CGCG 1 cut(s) 346
BstV1I GCAGC 4 cut(s) 199, 460, 2108, 3040
BstV2I GAAGAC 2 cut(s) 696, 743
BstX2I RGATCY 2 cut(s) 461, 1828
BstXI CCANNNNNNTGG 1 cut(s) 818
BstYI RGATCY 2 cut(s) 461, 1828
BstZ17I GTATAC 1 cut(s) 899
BsuI GTATCC 1 cut(s) 1325
BsuRI GGCC 5 cut(s) 53, 666, 723, 1151, 1782
BtgI CCRYGG 1 cut(s) 1921
BtgZI GCGATG 1 cut(s) 2890
BtsCI GGATG 8 cut(s) 619, 830, 1235, 1619, 2094, 2155, 2791, 2929
BtsI GCAGTG 1 cut(s) 1587
BtsIMutI CAGTG 4 cut(s) 748, 895, 1587, 2286
BveI ACCTGC 1 cut(s) 478
CciI TCATGA 1 cut(s) 2953
CfoI GCGC 2 cut(s) 348, 1879
Cfr13I GGNCC 9 cut(s) 191, 411, 721, 821, 1038, 1548, 1637, 1781, 1943
CspCI CAANNNNNGTGG 2 cut(s) 871, 906
DpnI GATC 6 cut(s) 463, 510, 620, 1351, 1830, 2952
DpnII GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
DraI TTTAAA 1 cut(s) 2871
DraIII CACNNNGTG 1 cut(s) 1058
DrdI GACNNNNNNGTC 1 cut(s) 82
DriI GACNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 82
EaeI YGGCCR 2 cut(s) 51, 1149
Eam1104I CTCTTC 3 cut(s) 96, 2773, 3024
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 3 cut(s) 96, 2773, 3024
Ecl136II GAGCTC 2 cut(s) 11, 1822
Eco130I CCWWGG 4 cut(s) 1387, 1758, 1921, 2451
Eco24I GRGCYC 3 cut(s) 13, 240, 1824
Eco47I GGWCC 7 cut(s) 191, 411, 821, 1038, 1548, 1637, 1943
Eco47III AGCGCT 1 cut(s) 1878
Eco53kI GAGCTC 2 cut(s) 11, 1822
Eco57I CTGAAG 2 cut(s) 590, 2523
Eco88I CYCGRG 1 cut(s) 2483
EcoICRI GAGCTC 2 cut(s) 11, 1822
EcoO109I RGGNCCY 1 cut(s) 411
EcoRI GAATTC 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 1815, 2083
EcoT14I CCWWGG 4 cut(s) 1387, 1758, 1921, 2451
EcoT38I GRGCYC 3 cut(s) 13, 240, 1824
ErhI CCWWGG 4 cut(s) 1387, 1758, 1921, 2451
FalI AAGNNNNNCTT 2 cut(s) 2280, 2312
FaqI GGGAC 1 cut(s) 1602
FauNDI CATATG 1 cut(s) 2120
FblI GTMKAC 2 cut(s) 149, 898
Fnu4HI GCNGC 4 cut(s) 213, 474, 2097, 3054
FokI GGATG 8 cut(s) 626, 817, 1222, 1606, 2101, 2162, 2798, 2936
FriOI GRGCYC 3 cut(s) 13, 240, 1824
Fsp4HI GCNGC 4 cut(s) 213, 474, 2097, 3054
GlaI GCGC 2 cut(s) 347, 1878
GluI GCNGC 4 cut(s) 213, 474, 2097, 3054
GsuI CTGGAG 3 cut(s) 1107, 1838, 2730
HaeII RGCGCY 1 cut(s) 1880
HaeIII GGCC 5 cut(s) 53, 666, 723, 1151, 1782
HapII CCGG 2 cut(s) 257, 611
HhaI GCGC 2 cut(s) 348, 1879
Hin6I GCGC 2 cut(s) 346, 1877
HinP1I GCGC 2 cut(s) 346, 1877
HincII GTYRAC 5 cut(s) 364, 880, 1104, 1445, 1462
HindII GTYRAC 5 cut(s) 364, 880, 1104, 1445, 1462
HpaI GTTAAC 3 cut(s) 364, 880, 1104
HpaII CCGG 2 cut(s) 257, 611
Hpy188I TCNGA 6 cut(s) 513, 771, 1899, 2196, 2503, 2902
HpyAV CCTTC 6 cut(s) 1512, 1772, 2125, 2585, 2756, 3085
HpyCH4III ACNGT 8 cut(s) 308, 737, 1292, 1318, 1570, 2513, 2731, 2795
HpyF10VI GCNNNNNNNGC 7 cut(s) 95, 104, 643, 652, 1305, 2159, 2660
HspAI GCGC 2 cut(s) 346, 1877
Kpn2I TCCGGA 2 cut(s) 256, 610
KspAI GTTAAC 3 cut(s) 364, 880, 1104
Kzo9I GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 4 cut(s) 259, 1819, 2478, 2749
Lsp1109I GCAGC 4 cut(s) 199, 460, 2108, 3040
LweI GCATC 6 cut(s) 1894, 2009, 2140, 2338, 2567, 2893
MalI GATC 6 cut(s) 463, 510, 620, 1351, 1830, 2952
MboI GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
MfeI CAATTG 1 cut(s) 2598
MflI RGATCY 2 cut(s) 461, 1828
MhlI GDGCHC 4 cut(s) 13, 61, 240, 1824
MlsI TGGCCA 2 cut(s) 53, 1151
MluNI TGGCCA 2 cut(s) 53, 1151
MlyI GAGTC 2 cut(s) 216, 1874
MmeI TCCRAC 5 cut(s) 165, 1382, 1636, 1958, 2547
Mox20I TGGCCA 2 cut(s) 53, 1151
MroI TCCGGA 2 cut(s) 256, 610
MroXI GAANNNNTTC 5 cut(s) 271, 452, 2190, 2296, 2574
MscI TGGCCA 2 cut(s) 53, 1151
MslI CAYNNNNRTG 1 cut(s) 2346
Msp20I TGGCCA 2 cut(s) 53, 1151
MspA1I CMGCKG 1 cut(s) 637
MspI CCGG 2 cut(s) 257, 611
MspR9I CCNGG 2 cut(s) 1817, 2085
MunI CAATTG 1 cut(s) 2598
MvaI CCWGG 2 cut(s) 1817, 2085
MvnI CGCG 1 cut(s) 346
MwoI GCNNNNNNNGC 7 cut(s) 95, 104, 643, 652, 1305, 2159, 2660
NcoI CCATGG 1 cut(s) 1921
NdeI CATATG 1 cut(s) 2120
NdeII GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
NlaIV GGNNCC 6 cut(s) 412, 463, 1039, 1549, 2480, 2745
NmuCI GTSAC 6 cut(s) 112, 119, 1011, 1672, 2704, 3058
NspI RCATGY 3 cut(s) 703, 2470, 2660
PaeR7I CTCGAG 1 cut(s) 2483
PagI TCATGA 1 cut(s) 2953
PciI ACATGT 2 cut(s) 699, 2656
PciSI GCTCTTC 1 cut(s) 96
PdmI GAANNNNTTC 5 cut(s) 271, 452, 2190, 2296, 2574
PfeI GAWTC 8 cut(s) 220, 394, 448, 772, 1854, 2699, 2888, 3020
PflMI CCANNNNNTGG 2 cut(s) 1940, 2753
PkrI GCNGC 4 cut(s) 214, 475, 2098, 3055
PleI GAGTC 2 cut(s) 215, 1873
PpsI GAGTC 2 cut(s) 215, 1873
PpuMI RGGWCCY 1 cut(s) 411
PscI ACATGT 2 cut(s) 699, 2656
Psp124BI GAGCTC 2 cut(s) 13, 1824
Psp5II RGGWCCY 1 cut(s) 411
Psp6I CCWGG 2 cut(s) 1815, 2083
PspGI CCWGG 2 cut(s) 1815, 2083
PspN4I GGNNCC 6 cut(s) 412, 463, 1039, 1549, 2480, 2745
PspPI GGNCC 9 cut(s) 191, 411, 721, 821, 1038, 1548, 1637, 1781, 1943
PspPPI RGGWCCY 1 cut(s) 411
PstI CTGCAG 3 cut(s) 1582, 3019, 3058
PsuI RGATCY 2 cut(s) 461, 1828
PvuII CAGCTG 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 2346
SacI GAGCTC 2 cut(s) 13, 1824
SapI GCTCTTC 1 cut(s) 96
SatI GCNGC 4 cut(s) 213, 474, 2097, 3054
Sau3AI GATC 6 cut(s) 461, 508, 618, 1349, 1828, 2950
Sau96I GGNCC 9 cut(s) 191, 411, 721, 821, 1038, 1548, 1637, 1781, 1943
ScaI AGTACT 2 cut(s) 2808, 2910
SchI GAGTC 2 cut(s) 216, 1874
ScrFI CCNGG 2 cut(s) 1817, 2085
SduI GDGCHC 4 cut(s) 13, 61, 240, 1824
SfaNI GCATC 6 cut(s) 1894, 2009, 2140, 2338, 2567, 2893
SfcI CTRYAG 6 cut(s) 1376, 1578, 2727, 2838, 3015, 3054
Sfr274I CTCGAG 1 cut(s) 2483
SinI GGWCC 7 cut(s) 191, 411, 821, 1038, 1548, 1637, 1943
SlaI CTCGAG 1 cut(s) 2483
SmiMI CAYNNNNRTG 1 cut(s) 2346
SmlI CTYRAG 5 cut(s) 126, 295, 1220, 1303, 2483
SmoI CTYRAG 5 cut(s) 126, 295, 1220, 1303, 2483
SpeI ACTAGT 1 cut(s) 116
SsiI CCGC 2 cut(s) 974, 2312
SspI AATATT 2 cut(s) 910, 2042
SstI GAGCTC 2 cut(s) 13, 1824
StyD4I CCNGG 2 cut(s) 1815, 2083
StyI CCWWGG 4 cut(s) 1387, 1758, 1921, 2451
TaaI ACNGT 8 cut(s) 308, 737, 1292, 1318, 1570, 2513, 2731, 2795
TaqI TCGA 3 cut(s) 1737, 1776, 2484
TaqII GACCGA 1 cut(s) 2723
TatI WGTACW 6 cut(s) 795, 1395, 2035, 2790, 2806, 2908
TfiI GAWTC 8 cut(s) 220, 394, 448, 772, 1854, 2699, 2888, 3020
TscAI CASTG 4 cut(s) 748, 895, 1587, 2286
TseFI GTSAC 6 cut(s) 112, 119, 1011, 1672, 2704, 3058
TseI GCWGC 4 cut(s) 212, 473, 2096, 3053
Tsp45I GTSAC 6 cut(s) 112, 119, 1011, 1672, 2704, 3058
TspDTI ATGAA 7 cut(s) 264, 1449, 1785, 2049, 2107, 2259, 2567
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 4 cut(s) 748, 895, 1587, 2286
Van91I CCANNNNNTGG 2 cut(s) 1940, 2753
VpaK11BI GGWCC 7 cut(s) 191, 411, 821, 1038, 1548, 1637, 1943
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 3 cut(s) 703, 2470, 2660
XcmI CCANNNNNNNNNTGG 4 cut(s) 837, 1246, 1581, 2995
XhoI CTCGAG 1 cut(s) 2483
XmiI GTMKAC 2 cut(s) 149, 898
XmnI GAANNNNTTC 5 cut(s) 271, 452, 2190, 2296, 2574
ZrmI AGTACT 2 cut(s) 2808, 2910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.