RchiOBHm_Chr5g0024401

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000922
Physical Location & Seq
Reverse (-)
18549979 .. 18554540
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30415

Sequence Viewer

Length: 1929 bp
ATGTCTTCAATCCCCAGAGACGAAGCAGCCTCGATTCAATGGCAGAAGGGCGATTTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAATACCGAATCCGGAGAGCTTATTGCTGCAAAAGAGGTTTTGGTATTGGTCCAAGAGGGGTTTTCCCAATTGAATCCGGAGAGCTTATTTGCTATTAGTGAGGCTCATACCAAGGTGCTTCAAGCTTTAGAAAACCTGTCGCATCCGAAAATTATTAGATGTTTAGGAACCGAGAGGGAGGATGAAACCTTAACTATGCTGTTGGAATATGTACCTGGAGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTCTCTGTTCCTGTGATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTAGAATATTTACACAAAAAAGGAATCATACATGGGGACATTAAGGCAGCAAATATCCTTGTTGATAATAAAGGGCGCATTAAACTAGCAGATTTTGGTGCATCCAAACAGGTGCATTTCAGGCAGCCGTTTCAGGTGCAAAGCCTACAAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTTGTGGGACTGGTCATGACTTATATATGGAGCGTTGGATGCACTGTGATTGAGATGGCCACTGGAATTCCTCCTTGGAACCAACAGTTTGAAACGCATCCTTTTTTAACTGGGAAAGATGTGGAATCTCAGAATTCTGCAGTCATGGAAGTAGAAGTAGCATCCTCATTTTGCCAAACAAATTCTGACGAAATTGATAGCATGTGGGATGATGATGCAAACTCCAATTTTGTATCCAATCCAGCAAGTTGCGGAACTAGAAAATTGATTGTGCAGGGAGCCATTAAACAAGTGTTTTCAAAAAAATTTAAATTCTTTAAGAGGTCGAGAAGAACTACCAGGAATGGACCAGCGAAGGGTGAGTTCAGCTTTGCATCTATCAAGGTTGCCACACACAACTTTTCTGAGGAAAATTTGATTGGACAAGGGGGATTTGGACCTGTTTATAAGGGAAAATTGGTGACCGGACAAGAAGTAGCTGTGAAAAGCCTTTCATCATCTTCACATCAAGGAGATAAGGAGTTTAAGAATGAACTGAGACTCATGCAGAAACTCCAACATATAAACATTATTCAGATATTAGGATTTTGCATTCATGGTGGCGGAAGATTTATAATATACGAGTACATGGCAAACTCAAGTTTAAACAACCATTTATTTGGTTCATCCAGTGACTTGCTACTAGATTGGAATAAGCGTTTCAACATAATTGAAGGAATTTCTCAAGGATTGGTTTACTCGCACAAATACTCCAGATTGAGAGTAATTCATAGAGATATAAAAGCCAGTAATGTTCTGCTGGATGCACAAATGAATCCCAAAATTGCTGATTTTGGTTTAGCAAAAATTTTTGCACATGATGAAGAGACAGTAAAAATTACGGGAATTTGTGGGACAATCGGTTACATGCCTCCCGAGTACCTCATGGCAGGCATCCTTTCCATGAAAACCAATGTCTACAGTTATGGAGTGTTAATGCTTGAAATCATAAGTGGTAACAAAAATTTCAATGCTGACGGCGATTATTTGGTAAAAGATGCACGGCAGTTATGGCAAAAAGGTGCAGGGCTACAACTAGTGGATCCAAAACTGGAAGTTTCTCGTATTGACAAACATCAACTGTTAAGATGCATCCATGTTGGTCTGCTGTGCGCGGAAGAAAATGAAGATGACCGGCCCACCATGTCAGATGCGATATCTATGTTGAAAAATGAAGTGCCTTTGCCTGAACCAACAATGCCAGCATTTTCTAGATACAATAATTGGACAACTGCTAGTATGGAAACTATGTGCATATATGGTTTATCAATAAACAGTATGACGATTTCCGACTTTGGTGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

642

Amino Acids

71.45

Weight (kDa)

7.01

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 235 1.4e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 222 4.4e-26 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 70 - 174 6.3e-08 ABC1 atypical kinase-like domain
Pkinase PF00069 329 - 593 2.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 331 - 592 4.4e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1029, 1196
AccI GTMKAC 1 cut(s) 1539
AccII CGCG 1 cut(s) 1736
AccIII TCCGGA 2 cut(s) 107, 172
AciI CCGC 3 cut(s) 834, 1185, 1736
AclWI GGATC 5 cut(s) 312, 325, 355, 1658, 1671
AcoI YGGCCR 2 cut(s) 76, 639
AcuI CTGAAG 1 cut(s) 596
AfaI GTAC 4 cut(s) 309, 555, 1208, 1502
AfiI CCNNNNNNNGG 2 cut(s) 550, 938
AhlI ACTAGT 1 cut(s) 1657
AjnI CCWGG 2 cut(s) 310, 920
AjuI GAANNNNNNNTTGG 4 cut(s) 640, 672, 984, 1016
AluBI AGCT 5 cut(s) 115, 180, 221, 951, 1061
AluI AGCT 5 cut(s) 115, 180, 221, 951, 1061
Alw26I GTCTC 3 cut(s) 12, 1114, 1442
AlwI GGATC 5 cut(s) 312, 325, 355, 1658, 1671
Ama87I CYCGRG 1 cut(s) 1496
Aor13HI TCCGGA 2 cut(s) 107, 172
AoxI GGCC 4 cut(s) 69, 76, 639, 1757
ApeKI GCWGC 4 cut(s) 26, 122, 446, 523
AspLEI GCGC 2 cut(s) 477, 1736
AspS9I GGNCC 5 cut(s) 69, 145, 929, 1019, 1758
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 3 cut(s) 370, 953, 1054
AvaI CYCGRG 1 cut(s) 1496
AvaII GGWCC 3 cut(s) 145, 929, 1019
BalI TGGCCA 1 cut(s) 641
BamHI GGATCC 2 cut(s) 317, 1663
BbvI GCAGC 4 cut(s) 38, 109, 458, 535
BccI CCATC 2 cut(s) 561, 631
BceAI ACGGC 4 cut(s) 63, 511, 1615, 1640
BciT130I CCWGG 2 cut(s) 312, 922
BciVI GTATCC 1 cut(s) 826
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 12, 1114, 1442
BcuI ACTAGT 1 cut(s) 1657
BfaI CTAG 7 cut(s) 401, 485, 840, 1265, 1658, 1833, 1857
BfmI CTRYAG 2 cut(s) 720, 1540
BfuI GTATCC 1 cut(s) 826
BisI GCNGC 4 cut(s) 27, 123, 447, 524
BlsI GCNGC 4 cut(s) 28, 124, 448, 525
Bme1390I CCNGG 3 cut(s) 67, 312, 922
Bme18I GGWCC 3 cut(s) 145, 929, 1019
BmeT110I CYCGRG 1 cut(s) 1496
BmgT120I GGNCC 5 cut(s) 69, 145, 929, 1019, 1758
BmiI GGNNCC 8 cut(s) 64, 70, 265, 319, 572, 662, 862, 1665
BmrFI CCNGG 3 cut(s) 67, 312, 922
BmrI ACTGGG 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 702
BpmI CTGGAG 2 cut(s) 333, 1318
BpuEI CTTGAG 2 cut(s) 1204, 1290
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 66, 207, 656
BsaWI WCCGGW 3 cut(s) 107, 172, 1046
BsaXI ACNNNNNCTCC 2 cut(s) 1316, 1346
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 938
Bse118I RCCGGY 1 cut(s) 1755
Bse1I ACTGG 7 cut(s) 568, 597, 649, 697, 1251, 1368, 1677
BseAI TCCGGA 2 cut(s) 107, 172
BseBI CCWGG 2 cut(s) 312, 922
BseDI CCNNGG 3 cut(s) 66, 207, 656
BseLI CCNNNNNNNGG 2 cut(s) 550, 938
BseMII CTCAG 3 cut(s) 725, 978, 1109
BseNI ACTGG 7 cut(s) 568, 597, 649, 697, 1251, 1368, 1677
BseXI GCAGC 4 cut(s) 38, 109, 458, 535
BsgI GTGCAG 2 cut(s) 875, 1665
Bsh1236I CGCG 1 cut(s) 1736
BshFI GGCC 4 cut(s) 71, 78, 641, 1759
BsiHKCI CYCGRG 1 cut(s) 1496
BsiSI CCGG 5 cut(s) 66, 108, 173, 1047, 1756
BslFI GGGAC 3 cut(s) 449, 603, 1489
BslI CCNNNNNNNGG 2 cut(s) 550, 938
BsmAI GTCTC 3 cut(s) 12, 1114, 1442
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 449, 603, 1489
BsmI GAATGC 1 cut(s) 1173
BsnI GGCC 4 cut(s) 71, 78, 641, 1759
BsoBI CYCGRG 1 cut(s) 1496
Bsp13I TCCGGA 2 cut(s) 107, 172
Bsp143I GATC 4 cut(s) 57, 317, 347, 1663
BspACI CCGC 3 cut(s) 834, 1185, 1736
BspANI GGCC 4 cut(s) 71, 78, 641, 1759
BspCNI CTCAG 3 cut(s) 724, 979, 1110
BspEI TCCGGA 2 cut(s) 107, 172
BspFNI CGCG 1 cut(s) 1736
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 8 cut(s) 64, 70, 265, 319, 572, 662, 862, 1665
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 5 cut(s) 312, 325, 355, 1658, 1671
BsrFI RCCGGY 1 cut(s) 1755
BsrI ACTGG 7 cut(s) 568, 597, 649, 697, 1251, 1368, 1677
BssAI RCCGGY 1 cut(s) 1755
BssECI CCNNGG 3 cut(s) 66, 207, 656
BssMI GATC 4 cut(s) 57, 317, 347, 1663
BssT1I CCWWGG 2 cut(s) 207, 656
Bst2UI CCWGG 2 cut(s) 312, 922
Bst4CI ACNGT 6 cut(s) 628, 669, 1453, 1544, 1704, 1898
Bst6I CTCTTC 1 cut(s) 1440
BstAPI GCANNNNNTGC 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 396, 1513, 1824
BstDEI CTNAG 3 cut(s) 711, 987, 1118
BstEII GGTNACC 1 cut(s) 1042
BstFNI CGCG 1 cut(s) 1736
BstHHI GCGC 2 cut(s) 477, 1736
BstKTI GATC 4 cut(s) 60, 320, 350, 1666
BstMAI GTCTC 3 cut(s) 12, 1114, 1442
BstMBI GATC 4 cut(s) 57, 317, 347, 1663
BstMWI GCNNNNNNNGC 4 cut(s) 391, 520, 621, 1633
BstNI CCWGG 2 cut(s) 312, 922
BstNSI RCATGY 2 cut(s) 787, 1492
BstPI GGTNACC 1 cut(s) 1042
BstSCI CCNGG 3 cut(s) 65, 310, 920
BstSFI CTRYAG 2 cut(s) 720, 1540
BstUI CGCG 1 cut(s) 1736
BstV1I GCAGC 4 cut(s) 38, 109, 458, 535
BstX2I RGATCY 3 cut(s) 317, 347, 1663
BstXI CCANNNNNNTGG 1 cut(s) 1241
BstYI RGATCY 3 cut(s) 317, 347, 1663
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 4 cut(s) 71, 78, 641, 1759
BtsIMutI CAGTG 3 cut(s) 624, 642, 1258
Cac8I GCNNGC 3 cut(s) 396, 1513, 1824
CciI TCATGA 1 cut(s) 597
CfoI GCGC 2 cut(s) 477, 1736
Cfr10I RCCGGY 1 cut(s) 1755
Cfr13I GGNCC 5 cut(s) 69, 145, 929, 1019, 1758
Csp6I GTAC 4 cut(s) 308, 554, 1207, 1501
CviQI GTAC 4 cut(s) 308, 554, 1207, 1501
DdeI CTNAG 3 cut(s) 711, 987, 1118
DpnI GATC 4 cut(s) 59, 319, 349, 1665
DpnII GATC 4 cut(s) 57, 317, 347, 1663
DraI TTTAAA 2 cut(s) 892, 1227
EaeI YGGCCR 2 cut(s) 76, 639
Eam1104I CTCTTC 1 cut(s) 1440
EarI CTCTTC 1 cut(s) 1440
EciI GGCGGA 1 cut(s) 1200
Eco130I CCWWGG 2 cut(s) 207, 656
Eco32I GATATC 1 cut(s) 1779
Eco47I GGWCC 3 cut(s) 145, 929, 1019
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 1496
Eco91I GGTNACC 1 cut(s) 1042
EcoO109I RGGNCCY 1 cut(s) 69
EcoO65I GGTNACC 1 cut(s) 1042
EcoRI GAATTC 2 cut(s) 648, 715
EcoRII CCWGG 2 cut(s) 310, 920
EcoRV GATATC 1 cut(s) 1779
EcoT14I CCWWGG 2 cut(s) 207, 656
EcoT22I ATGCAT 1 cut(s) 1715
ErhI CCWWGG 2 cut(s) 207, 656
Esp3I CGTCTC 1 cut(s) 12
FaqI GGGAC 3 cut(s) 449, 603, 1489
FblI GTMKAC 1 cut(s) 1539
Fnu4HI GCNGC 4 cut(s) 27, 123, 447, 524
Fsp4HI GCNGC 4 cut(s) 27, 123, 447, 524
FspBI CTAG 7 cut(s) 401, 485, 840, 1265, 1658, 1833, 1857
GlaI GCGC 2 cut(s) 476, 1735
GluI GCNGC 4 cut(s) 27, 123, 447, 524
GsuI CTGGAG 2 cut(s) 333, 1318
HaeIII GGCC 4 cut(s) 71, 78, 641, 1759
HapII CCGG 5 cut(s) 66, 108, 173, 1047, 1756
HhaI GCGC 2 cut(s) 477, 1736
Hin6I GCGC 2 cut(s) 475, 1734
HinP1I GCGC 2 cut(s) 475, 1734
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 8 cut(s) 34, 104, 169, 375, 423, 707, 1122, 1396
HpaII CCGG 5 cut(s) 66, 108, 173, 1047, 1756
HphI GGTGA 3 cut(s) 370, 953, 1054
Hpy166II GTNNAC 5 cut(s) 85, 556, 1318, 1540, 1922
Hpy188I TCNGA 7 cut(s) 243, 714, 769, 988, 1158, 1771, 1912
Hpy188III TCNNGA 8 cut(s) 108, 173, 574, 598, 909, 1335, 1496, 1833
Hpy8I GTNNAC 5 cut(s) 85, 556, 1318, 1540, 1922
HpyAV CCTTC 3 cut(s) 40, 931, 1289
HpyCH4III ACNGT 6 cut(s) 628, 669, 1453, 1544, 1704, 1898
HpyCH4IV ACGT 1 cut(s) 1924
HpyF10VI GCNNNNNNNGC 4 cut(s) 391, 520, 621, 1633
HpyF3I CTNAG 3 cut(s) 711, 987, 1118
HpySE526I ACGT 1 cut(s) 1924
HspAI GCGC 2 cut(s) 475, 1734
Kpn2I TCCGGA 2 cut(s) 107, 172
Kzo9I GATC 4 cut(s) 57, 317, 347, 1663
LmnI GCTCC 3 cut(s) 576, 612, 860
Lsp1109I GCAGC 4 cut(s) 38, 109, 458, 535
MaeI CTAG 7 cut(s) 401, 485, 840, 1265, 1658, 1833, 1857
MaeII ACGT 1 cut(s) 1924
MaeIII GTNAC 4 cut(s) 1042, 1253, 1484, 1577
MalI GATC 4 cut(s) 59, 319, 349, 1665
MboI GATC 4 cut(s) 57, 317, 347, 1663
MboII GAAGA 6 cut(s) 924, 1074, 1200, 1457, 1751, 1760
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 3 cut(s) 317, 347, 1663
MlsI TGGCCA 1 cut(s) 641
MluNI TGGCCA 1 cut(s) 641
MlyI GAGTC 1 cut(s) 1116
MmeI TCCRAC 3 cut(s) 279, 598, 1162
Mox20I TGGCCA 1 cut(s) 641
Mph1103I ATGCAT 1 cut(s) 1715
MroI TCCGGA 2 cut(s) 107, 172
MscI TGGCCA 1 cut(s) 641
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 641
MspI CCGG 5 cut(s) 66, 108, 173, 1047, 1756
MspR9I CCNGG 3 cut(s) 67, 312, 922
MssI GTTTAAAC 1 cut(s) 1227
MunI CAATTG 1 cut(s) 164
Mva1269I GAATGC 1 cut(s) 1173
MvaI CCWGG 2 cut(s) 312, 922
MvnI CGCG 1 cut(s) 1736
MwoI GCNNNNNNNGC 4 cut(s) 391, 520, 621, 1633
NciI CCSGG 1 cut(s) 67
NdeII GATC 4 cut(s) 57, 317, 347, 1663
NlaIV GGNNCC 8 cut(s) 64, 70, 265, 319, 572, 662, 862, 1665
NmuCI GTSAC 2 cut(s) 1042, 1253
NsiI ATGCAT 1 cut(s) 1715
NspI RCATGY 2 cut(s) 787, 1492
PagI TCATGA 1 cut(s) 597
PctI GAATGC 1 cut(s) 1173
PfeI GAWTC 7 cut(s) 34, 104, 169, 375, 423, 707, 1396
PkrI GCNGC 4 cut(s) 28, 124, 448, 525
PleI GAGTC 1 cut(s) 1116
PmeI GTTTAAAC 1 cut(s) 1227
PpsI GAGTC 1 cut(s) 1116
PsiI TTATAA 2 cut(s) 1029, 1196
Psp6I CCWGG 2 cut(s) 310, 920
PspEI GGTNACC 1 cut(s) 1042
PspGI CCWGG 2 cut(s) 310, 920
PspN4I GGNNCC 8 cut(s) 64, 70, 265, 319, 572, 662, 862, 1665
PspPI GGNCC 5 cut(s) 69, 145, 929, 1019, 1758
PstI CTGCAG 1 cut(s) 724
PsuI RGATCY 3 cut(s) 317, 347, 1663
RsaI GTAC 4 cut(s) 309, 555, 1208, 1502
RsaNI GTAC 4 cut(s) 308, 554, 1207, 1501
RseI CAYNNNNRTG 1 cut(s) 92
SatI GCNGC 4 cut(s) 27, 123, 447, 524
Sau3AI GATC 4 cut(s) 57, 317, 347, 1663
Sau96I GGNCC 5 cut(s) 69, 145, 929, 1019, 1758
SchI GAGTC 1 cut(s) 1116
ScrFI CCNGG 3 cut(s) 67, 312, 922
SfcI CTRYAG 2 cut(s) 720, 1540
SinI GGWCC 3 cut(s) 145, 929, 1019
SmiI ATTTAAAT 1 cut(s) 892
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 1219, 1305
SmoI CTYRAG 2 cut(s) 1219, 1305
SpeI ACTAGT 1 cut(s) 1657
SsiI CCGC 3 cut(s) 834, 1185, 1736
SspI AATATT 1 cut(s) 407
SspMI CTAG 7 cut(s) 401, 485, 840, 1265, 1658, 1833, 1857
StyD4I CCNGG 3 cut(s) 65, 310, 920
StyI CCWWGG 2 cut(s) 207, 656
SwaI ATTTAAAT 1 cut(s) 892
TaaI ACNGT 6 cut(s) 628, 669, 1453, 1544, 1704, 1898
TaiI ACGT 1 cut(s) 1927
TaqI TCGA 2 cut(s) 32, 908
TatI WGTACW 1 cut(s) 1206
TfiI GAWTC 7 cut(s) 34, 104, 169, 375, 423, 707, 1396
TscAI CASTG 3 cut(s) 631, 649, 1258
TseFI GTSAC 2 cut(s) 1042, 1253
TseI GCWGC 4 cut(s) 26, 122, 446, 523
Tsp45I GTSAC 2 cut(s) 1042, 1253
TspRI CASTG 3 cut(s) 631, 649, 1258
VpaK11BI GGWCC 3 cut(s) 145, 929, 1019
XbaI TCTAGA 1 cut(s) 1832
XceI RCATGY 2 cut(s) 787, 1492
XmiI GTMKAC 1 cut(s) 1539
XspI CTAG 7 cut(s) 401, 485, 840, 1265, 1658, 1833, 1857
Zsp2I ATGCAT 1 cut(s) 1715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.