RchiOBHm_Chr5g0024331

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000922
Physical Location & Seq
Reverse (-)
18519807 .. 18525316
5510 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30409

Sequence Viewer

Length: 1749 bp
ATGTCTTCAATGCCCCGAGACAAAGCAACCTCGATTCAATGGCGGAAGGGCGAATTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAATACTGAATCCGGAGAGCTTATTGCTGTAAAAGAGGTGTTGGTATTGGTATTGGAGGGGTTTTCCCAATTGAATCCGGAGAGCCTATTTGCTATTAGAGAGTGTTCTTCTTTTGTTACTAGTCAATTTAAATTGATTTGTCTTATGTGGAGGTTTTTCCTAATTAATTTCAGTCATGAAAGTTTTGGTTCTGCAGTTTGCTCATACCAACGTTCTTCAACAAGAAGTGAGGCTTTTAGAAAACCTGTCTCATCCGAAAATTGTTGTGAGTGGGAATTTCTTGTATCTGTTTTAACTAGTGATGACTTCCACATTTCTGAACCCGTGCTTGGTTTTGGTTTCTTAGAGATATTTAGGAACCGAGAGGGAGGACGAAACGTCGAAACCTTAACTATGCTGTTGGAATACGTACCTGGTGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTCTCTGTTCCTGGAGCAAATATCCTTGTTGATAAAAAATTAGGGCGCATCAAACTAGCAGATTTGGGTGCAACCAAACAGGCAGCCATTTCAAGTGCAAAGCCTACGAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTCGTGGGACTGGTCATGACTTATATATGGAGCGTTGGATGCACTCATCCTTTCTTAACTGGGAAAGAAGTGGAATCTCAGAATTCTGCCGTCAAGTAGAAGAAACTACAAGAAATAGATCGGCCAAAGGTGAGTTCAGCTATGCATCTATCATGGTGGCCACAGGCAATTTTTCTGAGCAAAATAAGATTGGACAAGGGGGATTTGGAGCTGTTTATACGGGAAAATTGGTGACCGGACAAGAAGTAGCAGTGAAAAGGCTTTCAAAATCTTCAAATCAAGGAAAATTGCAGTTTAAGAATGAACTGAGACTCATGCGTGAACTCCAACATAGAAACATTATTCGGATATTAGGATTTTGCATTCATGTTGACGAAAGAGTTCTAATATATGAGTACATGGCAAACGCAAGTTTGGACTACCATTTATTTGGTTCATCCAGTGACTTGCTACTAGATTGGAGTAAGTGTTTCAACATAATTGAAGGAATTGCTCAAGGATTGTTCTACTTGCACAAATACTCCAGACTGCTAATTCATAATGTAAAAGCTAGTAATGTTCTACTTGATGCACAAATGTATCCCAAAGTTGCTGATTTTGGTTTAGCAAAGATTTGTGCAGATGATGAAGAGACAGTAGAAACTGCGGCAATTTGTGGGACAATGGGTTACATGGCTCCCGAATACCTCATAACGGGCATCCTGTCCATGAAAACCTGTGGAAGTAAAGCCACTTGTGCATCTTACTTCATGCTTAAGTCAAAGATTGTGACTTACTTCATACTTAAGTTGTTGATAGGTCAAAGATTGTGGCTTACTTTCCAACATATGATAGTTTGTGCATTTCGTCCTGTTATAAGAGAGAGGGTATTCTCAATTGTTGCTCTCCTTATTGCAAAAGAGAAATTGTTGTATCCTTTTGCTATAGTGAAATCCTTCTACAATTTGCCTATCATCTTGTGCTTCACTCATTTTCATAGTTTCTACTTCAGTTGTAATTGGGTGCCCCATACCACAACGTTCCTAACAAAACTGATGTCTACAGCTATGGAGTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

582

Amino Acids

65.74

Weight (kDa)

8.71

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 189 - 243 1.6e-07 Protein kinase domain
Pkinase PF00069 289 - 460 9.2e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 291 - 468 2e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1547
AccB1I GGYRCC 1 cut(s) 1693
AccI GTMKAC 1 cut(s) 1730
AccIII TCCGGA 2 cut(s) 107, 172
AciI CCGC 2 cut(s) 43, 1337
AclI AACGTT 2 cut(s) 307, 1709
AclWI GGATC 3 cut(s) 510, 523, 553
AcoI YGGCCR 3 cut(s) 76, 813, 849
AcsI RAATTY 3 cut(s) 371, 539, 773
AcuI CTGAAG 2 cut(s) 705, 1663
AfaI GTAC 3 cut(s) 507, 664, 1088
AfiI CCNNNNNNNGG 3 cut(s) 425, 659, 1384
AflII CTTAAG 2 cut(s) 1445, 1475
AgsI TTSAA 9 cut(s) 9, 38, 169, 315, 642, 957, 966, 1165, 1175
AhdI GACNNNNNGTC 1 cut(s) 473
AhlI ACTAGT 2 cut(s) 215, 392
AjnI CCWGG 2 cut(s) 508, 559
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 809, 841
AluBI AGCT 5 cut(s) 115, 831, 902, 1241, 1736
AluI AGCT 5 cut(s) 115, 831, 902, 1241, 1736
Alw26I GTCTC 4 cut(s) 12, 349, 994, 1316
AlwI GGATC 3 cut(s) 510, 523, 553
Ama87I CYCGRG 1 cut(s) 15
Aor13HI TCCGGA 2 cut(s) 107, 172
AoxI GGCC 4 cut(s) 69, 76, 813, 849
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 3 cut(s) 371, 539, 773
ArsI GACNNNNNNTTYG 2 cut(s) 1712, 1744
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 3 cut(s) 690, 1071, 1625
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 1 cut(s) 69
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 2 cut(s) 833, 934
AvaI CYCGRG 1 cut(s) 15
BaeGI GKGCMC 1 cut(s) 1698
BaeI ACNNNNGTAYC 2 cut(s) 1253, 1286
BalI TGGCCA 1 cut(s) 851
BamHI GGATCC 1 cut(s) 515
BanI GGYRCC 1 cut(s) 1693
BbvI GCAGC 1 cut(s) 644
BccI CCATC 1 cut(s) 670
BceAI ACGGC 2 cut(s) 63, 765
BciT130I CCWGG 2 cut(s) 510, 561
BciVI GTATCC 2 cut(s) 1281, 1614
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 4 cut(s) 12, 349, 994, 1316
BcuI ACTAGT 2 cut(s) 215, 392
BfaI CTAG 5 cut(s) 216, 393, 605, 1145, 1242
BfmI CTRYAG 3 cut(s) 288, 1614, 1731
BfrI CTTAAG 2 cut(s) 1445, 1475
BfuI GTATCC 2 cut(s) 1281, 1614
BisI GCNGC 2 cut(s) 633, 1338
BlsI GCNGC 2 cut(s) 634, 1339
Bme1390I CCNGG 3 cut(s) 67, 510, 561
BmeRI GACNNNNNGTC 1 cut(s) 473
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 7 cut(s) 64, 70, 455, 517, 681, 1368, 1695
BmrFI CCNGG 3 cut(s) 67, 510, 561
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 6 cut(s) 606, 720, 845, 1249, 1398, 1439
BmuI ACTGGG 1 cut(s) 760
BpmI CTGGAG 2 cut(s) 582, 1198
BpuEI CTTGAG 1 cut(s) 1170
BpuMI CCSGG 1 cut(s) 67
BsaAI YACGTR 1 cut(s) 505
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 3 cut(s) 107, 172, 926
BsaXI ACNNNNNCTCC 4 cut(s) 1144, 1174, 1196, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 659, 1384
Bse1I ACTGG 4 cut(s) 677, 706, 755, 1131
BseAI TCCGGA 2 cut(s) 107, 172
BseBI CCWGG 2 cut(s) 510, 561
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 6 cut(s) 347, 681, 735, 737, 1127, 1389
BseLI CCNNNNNNNGG 3 cut(s) 425, 659, 1384
BseMII CTCAG 3 cut(s) 783, 858, 989
BseNI ACTGG 4 cut(s) 677, 706, 755, 1131
BseSI GKGCMC 1 cut(s) 1698
BseXI GCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 1329
BshFI GGCC 4 cut(s) 71, 78, 815, 851
BshNI GGYRCC 1 cut(s) 1693
BsiHKCI CYCGRG 1 cut(s) 15
BsiSI CCGG 4 cut(s) 66, 108, 173, 927
BslFI GGGAC 2 cut(s) 712, 1363
BslI CCNNNNNNNGG 3 cut(s) 425, 659, 1384
BsmAI GTCTC 4 cut(s) 12, 349, 994, 1316
BsmFI GGGAC 2 cut(s) 712, 1363
BsmI GAATGC 1 cut(s) 1053
BsnI GGCC 4 cut(s) 71, 78, 815, 851
BsoBI CYCGRG 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 1698
Bsp13I TCCGGA 2 cut(s) 107, 172
Bsp143I GATC 4 cut(s) 57, 515, 545, 809
BspACI CCGC 2 cut(s) 43, 1337
BspANI GGCC 4 cut(s) 71, 78, 815, 851
BspCNI CTCAG 3 cut(s) 782, 859, 990
BspEI TCCGGA 2 cut(s) 107, 172
BspHI TCATGA 2 cut(s) 271, 706
BspLI GGNNCC 7 cut(s) 64, 70, 455, 517, 681, 1368, 1695
BspMAI CTGCAG 1 cut(s) 292
BspPI GGATC 3 cut(s) 510, 523, 553
BspT107I GGYRCC 1 cut(s) 1693
BspTI CTTAAG 2 cut(s) 1445, 1475
BsrI ACTGG 4 cut(s) 677, 706, 755, 1131
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 4 cut(s) 57, 515, 545, 809
Bst2UI CCWGG 2 cut(s) 510, 561
Bst4CI ACNGT 1 cut(s) 1327
Bst6I CTCTTC 1 cut(s) 1314
BstAFI CTTAAG 2 cut(s) 1445, 1475
BstBAI YACGTR 1 cut(s) 505
BstDEI CTNAG 4 cut(s) 439, 769, 867, 998
BstEII GGTNACC 1 cut(s) 922
BstF5I GGATG 6 cut(s) 347, 681, 735, 737, 1127, 1389
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 4 cut(s) 60, 518, 548, 812
BstMAI GTCTC 4 cut(s) 12, 349, 994, 1316
BstMBI GATC 4 cut(s) 57, 515, 545, 809
BstMWI GCNNNNNNNGC 2 cut(s) 730, 1427
BstNI CCWGG 2 cut(s) 510, 561
BstPI GGTNACC 1 cut(s) 922
BstSCI CCNGG 3 cut(s) 65, 508, 559
BstSFI CTRYAG 3 cut(s) 288, 1614, 1731
BstSLI GKGCMC 1 cut(s) 1698
BstSNI TACGTA 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 644
BstX2I RGATCY 2 cut(s) 515, 545
BstXI CCANNNNNNTGG 1 cut(s) 1121
BstYI RGATCY 2 cut(s) 515, 545
BsuI GTATCC 2 cut(s) 1281, 1614
BsuRI GGCC 4 cut(s) 71, 78, 815, 851
BtsCI GGATG 6 cut(s) 347, 681, 735, 737, 1127, 1389
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 2 cut(s) 948, 1138
CciI TCATGA 2 cut(s) 271, 706
CfoI GCGC 1 cut(s) 597
Cfr13I GGNCC 1 cut(s) 69
CsiI ACCWGGT 1 cut(s) 508
Csp6I GTAC 3 cut(s) 506, 663, 1087
CspCI CAANNNNNGTGG 2 cut(s) 1481, 1516
CviQI GTAC 3 cut(s) 506, 663, 1087
DdeI CTNAG 4 cut(s) 439, 769, 867, 998
DpnI GATC 4 cut(s) 59, 517, 547, 811
DpnII GATC 4 cut(s) 57, 515, 545, 809
DraI TTTAAA 1 cut(s) 226
DriI GACNNNNNGTC 1 cut(s) 473
EaeI YGGCCR 3 cut(s) 76, 813, 849
Eam1104I CTCTTC 1 cut(s) 1314
Eam1105I GACNNNNNGTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 1314
EciI GGCGGA 1 cut(s) 58
Eco105I TACGTA 1 cut(s) 505
Eco57I CTGAAG 2 cut(s) 705, 1663
Eco88I CYCGRG 1 cut(s) 15
Eco91I GGTNACC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 69
EcoO65I GGTNACC 1 cut(s) 922
EcoRI GAATTC 1 cut(s) 773
EcoRII CCWGG 2 cut(s) 508, 559
EcoT22I ATGCAT 1 cut(s) 838
FalI AAGNNNNNCTT 2 cut(s) 313, 345
FaqI GGGAC 2 cut(s) 712, 1363
FauNDI CATATG 1 cut(s) 1518
FblI GTMKAC 1 cut(s) 1730
Fnu4HI GCNGC 2 cut(s) 633, 1338
FokI GGATG 6 cut(s) 334, 688, 724, 742, 1114, 1376
Fsp4HI GCNGC 2 cut(s) 633, 1338
FspBI CTAG 5 cut(s) 216, 393, 605, 1145, 1242
GlaI GCGC 1 cut(s) 596
GluI GCNGC 2 cut(s) 633, 1338
GsuI CTGGAG 2 cut(s) 582, 1198
HaeIII GGCC 4 cut(s) 71, 78, 815, 851
HapII CCGG 4 cut(s) 66, 108, 173, 927
HhaI GCGC 1 cut(s) 597
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HinfI GANTC 5 cut(s) 34, 104, 169, 765, 1002
HpaII CCGG 4 cut(s) 66, 108, 173, 927
HphI GGTGA 2 cut(s) 833, 934
Hpy166II GTNNAC 5 cut(s) 85, 665, 1013, 1063, 1731
Hpy188I TCNGA 5 cut(s) 352, 415, 772, 868, 1038
Hpy188III TCNNGA 7 cut(s) 108, 173, 272, 683, 707, 1215, 1370
Hpy8I GTNNAC 5 cut(s) 85, 665, 1013, 1063, 1731
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 40, 1169, 1636
HpyCH4III ACNGT 1 cut(s) 1327
HpyCH4IV ACGT 4 cut(s) 307, 474, 504, 1709
HpyF10VI GCNNNNNNNGC 2 cut(s) 730, 1427
HpyF3I CTNAG 4 cut(s) 439, 769, 867, 998
HpySE526I ACGT 4 cut(s) 307, 474, 504, 1709
HspAI GCGC 1 cut(s) 595
Kpn2I TCCGGA 2 cut(s) 107, 172
Kzo9I GATC 4 cut(s) 57, 515, 545, 809
LmnI GCTCC 5 cut(s) 563, 685, 721, 899, 1372
Lsp1109I GCAGC 1 cut(s) 644
LweI GCATC 6 cut(s) 606, 720, 845, 1249, 1398, 1439
MabI ACCWGGT 1 cut(s) 508
MaeI CTAG 5 cut(s) 216, 393, 605, 1145, 1242
MaeII ACGT 4 cut(s) 307, 474, 504, 1709
MaeIII GTNAC 5 cut(s) 211, 922, 1133, 1358, 1459
MalI GATC 4 cut(s) 59, 517, 547, 811
MboI GATC 4 cut(s) 57, 515, 545, 809
MboII GAAGA 5 cut(s) 195, 303, 803, 954, 1331
MfeI CAATTG 2 cut(s) 164, 1566
MflI RGATCY 2 cut(s) 515, 545
MhlI GDGCHC 1 cut(s) 1698
MlsI TGGCCA 1 cut(s) 851
MluNI TGGCCA 1 cut(s) 851
MlyI GAGTC 1 cut(s) 996
MmeI TCCRAC 4 cut(s) 477, 707, 1042, 1537
Mox20I TGGCCA 1 cut(s) 851
Mph1103I ATGCAT 1 cut(s) 838
MroI TCCGGA 2 cut(s) 107, 172
MroXI GAANNNNTTC 3 cut(s) 690, 1071, 1625
MscI TGGCCA 1 cut(s) 851
MseI TTAA 9 cut(s) 225, 261, 389, 485, 747, 987, 1446, 1476, 1747
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 851
MspCI CTTAAG 2 cut(s) 1445, 1475
MspI CCGG 4 cut(s) 66, 108, 173, 927
MspR9I CCNGG 3 cut(s) 67, 510, 561
MunI CAATTG 2 cut(s) 164, 1566
Mva1269I GAATGC 1 cut(s) 1053
MvaI CCWGG 2 cut(s) 510, 561
MwoI GCNNNNNNNGC 2 cut(s) 730, 1427
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1518
NdeII GATC 4 cut(s) 57, 515, 545, 809
NlaIV GGNNCC 7 cut(s) 64, 70, 455, 517, 681, 1368, 1695
NmuCI GTSAC 3 cut(s) 922, 1133, 1459
NsiI ATGCAT 1 cut(s) 838
PagI TCATGA 2 cut(s) 271, 706
PctI GAATGC 1 cut(s) 1053
PdmI GAANNNNTTC 3 cut(s) 690, 1071, 1625
PfeI GAWTC 4 cut(s) 34, 104, 169, 765
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 2 cut(s) 634, 1339
PleI GAGTC 1 cut(s) 996
PpsI GAGTC 1 cut(s) 996
Ppu21I YACGTR 1 cut(s) 505
PshBI ATTAAT 1 cut(s) 261
PsiI TTATAA 1 cut(s) 1547
Psp1406I AACGTT 2 cut(s) 307, 1709
Psp6I CCWGG 2 cut(s) 508, 559
PspEI GGTNACC 1 cut(s) 922
PspGI CCWGG 2 cut(s) 508, 559
PspN4I GGNNCC 7 cut(s) 64, 70, 455, 517, 681, 1368, 1695
PspPI GGNCC 1 cut(s) 69
PstI CTGCAG 1 cut(s) 292
PsuI RGATCY 2 cut(s) 515, 545
RsaI GTAC 3 cut(s) 507, 664, 1088
RsaNI GTAC 3 cut(s) 506, 663, 1087
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 9 cut(s) 225, 261, 389, 485, 747, 987, 1446, 1476, 1747
SatI GCNGC 2 cut(s) 633, 1338
Sau3AI GATC 4 cut(s) 57, 515, 545, 809
Sau96I GGNCC 1 cut(s) 69
SchI GAGTC 1 cut(s) 996
ScrFI CCNGG 3 cut(s) 67, 510, 561
SduI GDGCHC 1 cut(s) 1698
SexAI ACCWGGT 1 cut(s) 508
SfaNI GCATC 6 cut(s) 606, 720, 845, 1249, 1398, 1439
SfcI CTRYAG 3 cut(s) 288, 1614, 1731
SmiI ATTTAAAT 1 cut(s) 226
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 3 cut(s) 1185, 1445, 1475
SmoI CTYRAG 3 cut(s) 1185, 1445, 1475
SnaBI TACGTA 1 cut(s) 505
SpeI ACTAGT 2 cut(s) 215, 392
SsiI CCGC 2 cut(s) 43, 1337
SspMI CTAG 5 cut(s) 216, 393, 605, 1145, 1242
StyD4I CCNGG 3 cut(s) 65, 508, 559
SwaI ATTTAAAT 1 cut(s) 226
TaaI ACNGT 1 cut(s) 1327
TaiI ACGT 4 cut(s) 310, 477, 507, 1712
TaqI TCGA 2 cut(s) 32, 477
TatI WGTACW 1 cut(s) 1086
TauI GCSGC 1 cut(s) 1340
TfiI GAWTC 4 cut(s) 34, 104, 169, 765
Tru1I TTAA 9 cut(s) 225, 261, 389, 485, 747, 987, 1446, 1476, 1747
Tru9I TTAA 9 cut(s) 225, 261, 389, 485, 747, 987, 1446, 1476, 1747
TscAI CASTG 2 cut(s) 948, 1138
TseFI GTSAC 3 cut(s) 922, 1133, 1459
TseI GCWGC 1 cut(s) 632
Tsp45I GTSAC 3 cut(s) 922, 1133, 1459
TspRI CASTG 2 cut(s) 948, 1138
Vha464I CTTAAG 2 cut(s) 1445, 1475
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 3 cut(s) 371, 539, 773
XmiI GTMKAC 1 cut(s) 1730
XmnI GAANNNNTTC 3 cut(s) 690, 1071, 1625
XspI CTAG 5 cut(s) 216, 393, 605, 1145, 1242
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.