Rh5BG171100

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000922
Physical Location & Seq
Reverse (-)
17934419 .. 17943654
9236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG171100.1

Sequence Viewer

Length: 1044 bp
ATGTCTTCAATCCCCAGAGACGAAGCAGCCTCGATTCAATGGCAGAAGGGTGATTTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAATACCGAATCCGGAGAGCTTATTGCTGCAAAAGAGGCTCATACCAAGGTGCTTCAAGATGAAGTGAGGCTTTTAGAAAACCTGTCGCATCCGAAAATTATTAGATGTTTAGGAACCGAGAGGGAGGATGAAACCTTAACTATGCTGTTGGAATATGTACCTGGAGGATCCATACAAGCACTATTGGAGAAATTTGGATTTTTCTCTGTTCCTGTAATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTAGATTATTTACACAAAAAAGGAATCATACATGGGGACATTAAGGGAGCAAATATCCTTGTTGGTAATAAAGGGCGCATTAAACTAGCAGATTTTGGTGCATCCAAACAGGTGCATTTCAGGCAGCCATTTCGGGTGCAAAGCCTACAAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTCGTGGGACTGGACATGACTTATATATGGAGCGTTGGATGCACTGTGATTGAGATGGCCACTGGAAATCCTCCTTGGAACCAACAGTTTGAAACGGTTGCTACTTTCTTTTATTGTGTAGGGACAAGCAAGTCTCATCCACCAATCCCTGATCATCTTTCTCCTGCAGCAAAAGATTTTCTGCACGAATGTCTGCAAAAGGAACCAACTGTAAGGCCATCCGCATCTGAGTTGCTTAAGCATCCTTTTTTTACTGGGAAAGAAGTGGAATCTCAGAATTCTGCCGTCATGGGAAAATTGGTGACCGGACAAGAAGTAGCTGTGAAAAGCCTTTCATCATCTTCACATCAAGGAGATAAGGAGTTTAAGAATGAACTGAGACTCATGCAGAAACTCCAACATAGAAACATTATTCAGATATTAGGATTTTGCATTCATGGTGGCGGAAGATTTATAATATACGAGTACATGGCAAACACAAGTTTAAACAACCATTTATTTGGTGGGGGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

347

Amino Acids

38.59

Weight (kDa)

8.68

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 260 2.1e-54 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 256 1.3e-31 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 49 - 155 1.6e-07 ABC1 atypical kinase-like domain
Pkinase PF00069 277 - 342 7.7e-09 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 278 - 342 1e-12 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 983
AasI GACNNNNNNGTC 1 cut(s) 658
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 2 cut(s) 750, 972
AclWI GGATC 2 cut(s) 258, 271
AcoI YGGCCR 2 cut(s) 76, 585
AcsI RAATTY 2 cut(s) 287, 805
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 3 cut(s) 255, 501, 995
AfiI CCNNNNNNNGG 1 cut(s) 496
AflII CTTAAG 1 cut(s) 764
AgsI TTSAA 4 cut(s) 9, 38, 152, 620
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 2 cut(s) 115, 848
AluI AGCT 2 cut(s) 115, 848
Alw26I GTCTC 3 cut(s) 12, 666, 901
AlwI GGATC 2 cut(s) 258, 271
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 4 cut(s) 69, 76, 585, 743
ApeKI GCWGC 4 cut(s) 26, 122, 469, 695
ApoI RAATTY 2 cut(s) 287, 805
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 69
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 3 cut(s) 62, 316, 841
BalI TGGCCA 1 cut(s) 587
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 4 cut(s) 38, 109, 481, 707
BccI CCATC 3 cut(s) 507, 577, 754
BceAI ACGGC 2 cut(s) 63, 797
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 679
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 12, 666, 901
BfaI CTAG 2 cut(s) 347, 431
BfmI CTRYAG 1 cut(s) 693
BfrI CTTAAG 1 cut(s) 764
BisI GCNGC 4 cut(s) 27, 123, 470, 696
BlsI GCNGC 4 cut(s) 28, 124, 471, 697
Bme1390I CCNGG 2 cut(s) 67, 258
BmgT120I GGNCC 1 cut(s) 69
BmiI GGNNCC 7 cut(s) 64, 70, 211, 265, 518, 608, 732
BmrFI CCNGG 2 cut(s) 67, 258
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 5 cut(s) 193, 455, 557, 761, 778
BmuI ACTGGG 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 66, 141, 602
BsaWI WCCGGW 2 cut(s) 107, 833
Bsc4I CCNNNNNNNGG 1 cut(s) 496
Bse1I ACTGG 4 cut(s) 514, 543, 595, 787
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 66, 141, 602
BseGI GGATG 8 cut(s) 184, 229, 446, 518, 572, 664, 746, 769
BseLI CCNNNNNNNGG 1 cut(s) 496
BseMII CTCAG 3 cut(s) 747, 815, 896
BseNI ACTGG 4 cut(s) 514, 543, 595, 787
BseXI GCAGC 4 cut(s) 38, 109, 481, 707
BsgI GTGCAG 1 cut(s) 695
BshFI GGCC 4 cut(s) 71, 78, 587, 745
BsiSI CCGG 3 cut(s) 66, 108, 834
BslFI GGGAC 3 cut(s) 395, 549, 664
BslI CCNNNNNNNGG 1 cut(s) 496
BsmAI GTCTC 3 cut(s) 12, 666, 901
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 395, 549, 664
BsmI GAATGC 1 cut(s) 960
BsnI GGCC 4 cut(s) 71, 78, 587, 745
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 57, 263, 679
BspACI CCGC 2 cut(s) 750, 972
BspANI GGCC 4 cut(s) 71, 78, 587, 745
BspCNI CTCAG 3 cut(s) 748, 814, 897
BspEI TCCGGA 1 cut(s) 107
BspLI GGNNCC 7 cut(s) 64, 70, 211, 265, 518, 608, 732
BspMAI CTGCAG 1 cut(s) 697
BspPI GGATC 2 cut(s) 258, 271
BspTI CTTAAG 1 cut(s) 764
BsrI ACTGG 4 cut(s) 514, 543, 595, 787
BssECI CCNNGG 3 cut(s) 66, 141, 602
BssMI GATC 3 cut(s) 57, 263, 679
BssT1I CCWWGG 2 cut(s) 141, 602
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 4 cut(s) 574, 615, 625, 739
BstAFI CTTAAG 1 cut(s) 764
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 756, 801, 905
BstEII GGTNACC 1 cut(s) 829
BstF5I GGATG 8 cut(s) 184, 229, 446, 518, 572, 664, 746, 769
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 3 cut(s) 60, 266, 682
BstMAI GTCTC 3 cut(s) 12, 666, 901
BstMBI GATC 3 cut(s) 57, 263, 679
BstMWI GCNNNNNNNGC 4 cut(s) 131, 337, 466, 567
BstNI CCWGG 1 cut(s) 258
BstPI GGTNACC 1 cut(s) 829
BstSCI CCNGG 2 cut(s) 65, 256
BstSFI CTRYAG 1 cut(s) 693
BstV1I GCAGC 4 cut(s) 38, 109, 481, 707
BstX2I RGATCY 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 1028
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 4 cut(s) 71, 78, 587, 745
BtsCI GGATG 8 cut(s) 184, 229, 446, 518, 572, 664, 746, 769
BtsIMutI CAGTG 2 cut(s) 570, 588
Cac8I GCNNGC 1 cut(s) 342
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 69
Csp6I GTAC 3 cut(s) 254, 500, 994
CviAII CATG 8 cut(s) 88, 94, 377, 544, 817, 913, 965, 997
CviQI GTAC 3 cut(s) 254, 500, 994
DdeI CTNAG 3 cut(s) 756, 801, 905
DpnI GATC 3 cut(s) 59, 265, 681
DpnII GATC 3 cut(s) 57, 263, 679
DraI TTTAAA 1 cut(s) 1014
DrdI GACNNNNNNGTC 1 cut(s) 658
DseDI GACNNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 2 cut(s) 76, 585
EciI GGCGGA 1 cut(s) 987
Eco130I CCWWGG 2 cut(s) 141, 602
Eco57I CTGAAG 1 cut(s) 542
Eco91I GGTNACC 1 cut(s) 829
EcoO109I RGGNCCY 1 cut(s) 69
EcoO65I GGTNACC 1 cut(s) 829
EcoRI GAATTC 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 141, 602
ErhI CCWWGG 2 cut(s) 141, 602
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 8 cut(s) 91, 97, 380, 547, 820, 916, 968, 1000
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 3 cut(s) 395, 549, 664
FatI CATG 8 cut(s) 87, 93, 376, 543, 816, 912, 964, 996
FbaI TGATCA 1 cut(s) 679
Fnu4HI GCNGC 4 cut(s) 27, 123, 470, 696
FokI GGATG 8 cut(s) 171, 236, 433, 525, 579, 651, 733, 756
Fsp4HI GCNGC 4 cut(s) 27, 123, 470, 696
FspBI CTAG 2 cut(s) 347, 431
GlaI GCGC 1 cut(s) 422
GluI GCNGC 4 cut(s) 27, 123, 470, 696
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 4 cut(s) 71, 78, 587, 745
HapII CCGG 3 cut(s) 66, 108, 834
HhaI GCGC 1 cut(s) 423
Hin1II CATG 8 cut(s) 91, 97, 380, 547, 820, 916, 968, 1000
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HinfI GANTC 6 cut(s) 34, 104, 321, 369, 797, 909
HpaII CCGG 3 cut(s) 66, 108, 834
HphI GGTGA 3 cut(s) 62, 316, 841
Hpy166II GTNNAC 2 cut(s) 85, 502
Hpy188I TCNGA 4 cut(s) 189, 757, 804, 945
Hpy188III TCNNGA 3 cut(s) 108, 152, 520
Hpy8I GTNNAC 2 cut(s) 85, 502
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 4 cut(s) 574, 615, 625, 739
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 337, 466, 567
HpyF3I CTNAG 3 cut(s) 756, 801, 905
Hsp92II CATG 8 cut(s) 91, 97, 380, 547, 820, 916, 968, 1000
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 679
Kzo9I GATC 3 cut(s) 57, 263, 679
LmnI GCTCC 3 cut(s) 392, 522, 558
Lsp1109I GCAGC 4 cut(s) 38, 109, 481, 707
LweI GCATC 5 cut(s) 193, 455, 557, 761, 778
MaeI CTAG 2 cut(s) 347, 431
MaeIII GTNAC 1 cut(s) 829
MalI GATC 3 cut(s) 59, 265, 681
MboI GATC 3 cut(s) 57, 263, 679
MboII GAAGA 2 cut(s) 861, 987
MflI RGATCY 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 587
MluCI AATT 4 cut(s) 192, 287, 805, 824
MluNI TGGCCA 1 cut(s) 587
MlyI GAGTC 1 cut(s) 903
MmeI TCCRAC 3 cut(s) 225, 544, 949
MnlI CCTC 7 cut(s) 40, 124, 156, 210, 214, 254, 609
Mox20I TGGCCA 1 cut(s) 587
MroI TCCGGA 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 527
MscI TGGCCA 1 cut(s) 587
MseI TTAA 7 cut(s) 233, 387, 426, 765, 894, 1013, 1042
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 587
MspCI CTTAAG 1 cut(s) 764
MspI CCGG 3 cut(s) 66, 108, 834
MspR9I CCNGG 2 cut(s) 67, 258
MssI GTTTAAAC 1 cut(s) 1014
Mva1269I GAATGC 1 cut(s) 960
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 4 cut(s) 131, 337, 466, 567
NciI CCSGG 1 cut(s) 67
NdeII GATC 3 cut(s) 57, 263, 679
NlaIII CATG 8 cut(s) 91, 97, 380, 547, 820, 916, 968, 1000
NlaIV GGNNCC 7 cut(s) 64, 70, 211, 265, 518, 608, 732
NmuCI GTSAC 1 cut(s) 829
PctI GAATGC 1 cut(s) 960
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 5 cut(s) 34, 104, 321, 369, 797
PkrI GCNGC 4 cut(s) 28, 124, 471, 697
PleI GAGTC 1 cut(s) 903
PmeI GTTTAAAC 1 cut(s) 1014
PpsI GAGTC 1 cut(s) 903
PsiI TTATAA 1 cut(s) 983
Psp6I CCWGG 1 cut(s) 256
PspEI GGTNACC 1 cut(s) 829
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 7 cut(s) 64, 70, 211, 265, 518, 608, 732
PspPI GGNCC 1 cut(s) 69
PstI CTGCAG 1 cut(s) 697
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 3 cut(s) 255, 501, 995
RsaNI GTAC 3 cut(s) 254, 500, 994
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 7 cut(s) 233, 387, 426, 765, 894, 1013, 1042
SatI GCNGC 4 cut(s) 27, 123, 470, 696
Sau3AI GATC 3 cut(s) 57, 263, 679
Sau96I GGNCC 1 cut(s) 69
SchI GAGTC 1 cut(s) 903
ScrFI CCNGG 2 cut(s) 67, 258
SetI ASST 7 cut(s) 117, 147, 180, 233, 259, 459, 850
SfaNI GCATC 5 cut(s) 193, 455, 557, 761, 778
SfcI CTRYAG 1 cut(s) 693
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 4 cut(s) 192, 287, 805, 824
SsiI CCGC 2 cut(s) 750, 972
SspMI CTAG 2 cut(s) 347, 431
StyD4I CCNGG 2 cut(s) 65, 256
StyI CCWWGG 2 cut(s) 141, 602
TaaI ACNGT 4 cut(s) 574, 615, 625, 739
TaqI TCGA 1 cut(s) 32
TasI AATT 4 cut(s) 192, 287, 805, 824
TatI WGTACW 1 cut(s) 993
TfiI GAWTC 5 cut(s) 34, 104, 321, 369, 797
Tru1I TTAA 7 cut(s) 233, 387, 426, 765, 894, 1013, 1042
Tru9I TTAA 7 cut(s) 233, 387, 426, 765, 894, 1013, 1042
TscAI CASTG 2 cut(s) 577, 595
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 4 cut(s) 26, 122, 469, 695
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 6 cut(s) 110, 171, 240, 852, 915, 953
TspRI CASTG 2 cut(s) 577, 595
Vha464I CTTAAG 1 cut(s) 764
XapI RAATTY 2 cut(s) 287, 805
XcmI CCANNNNNNNNNTGG 1 cut(s) 1028
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 2 cut(s) 347, 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.