RchiOBHm_Chr5g0024321

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000922
Physical Location & Seq
Reverse (-)
18494798 .. 18498921
4124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30408

Sequence Viewer

Length: 1716 bp
ATGTCTTCAATCCAATGGCGGAAGGGCGATTTGATCGGTTCAGGGACCTTTGGCCGTGTTTACATGGGCATGGATACTGAATCCGGAGCGCTCATTGCTGCTAAAGAGGTCCAATTATTTTCATTGGCTTTGTGCTGTTTTGTGTTTGGTTATACGGATTTGGCTGAGTTGTTGGCTGACACTAAGGCCCTCCAAGAAGAAATGGAGCTTCTAGAAAACCTGTCTCATCCAAAAATTGTTTCTCTGGTTTTTAAGAGGTATTTGGGGATGGAAATGGAGGATGAAACCGTGACTATGTTGTTGGAATATGTCCCTGGTGGATCCATACAATCACTTCTCGAGAAATTTGGATCTTTCTCCATTCCTGGAGCAAATATCCTTGTTGATAATAAAGGACGCATTAAACTAGCAGATTTTGGGGCATCCAAACAGGTTGACTCCGCTGATATATGGAGTGTTGGATGTACTGTGATTGAGATGGCCATGGGAAATCCTCCTTGGAACAAACATTTCGAACGGGAACCAAATTTGAGGCCATCCGCGTCTGAGCTGCTTCAGCATCCTTTTTTAACTGGGAAACAAGGGAAATCTCAGTCTGGTGGTCATACTGCTTCTATGGAAATGAGTTCCTTTCAGGGGTGTCATGAAGTTATTAACCCCATCATGGAACTGGAAGGAGTGGTATCCATAGAGTCTGAAACATGTGATGAAGATATTGGTAGCACTTGGAGCGACCATAGTTCCGACTCCAATAATCCAGCCGCTCACGCCAATAAACATATCTTTTCAATATTCTTTAGAAAGTTTAAGAGACCAACAATAACTCCTAGTCATGGATCTACAAAGGGCAAGTTCAGCTATGCATCTATCAAGTTGGCCACATGCAACTTCTCTGAGGAAAACAAGCTCGGACAAGGGGGATTTGGATGTGTGTATAAGGGAATCGTGGAGACTGGACAACAGGTAGCTGTGAAAAGGCTTTCACTATATTCAGGGCAAGGATTTTCGGAGTTTAATAATGAACTGAGAACTATAGGTGAACTCCAACATAGAAACGTTATTCAGATTGTAGGATATTGCATTCATGGTGATGAGAGAATATTAATATACGAGTTCATGCCAAAAGTGAGTTTGGACTACTATTTACGAGATTCATCCAAAAGCTTGCTACTAGATTGGAGGAAGCGATTCAATATAATTAAAGGACTTACTCAAGGATTGGTTTACTTGCACGTATACTCCAGAATTAGAGGAATTCACAGAGATATAAAACCCACAAAAACTAGACGCATTGCTGGGACATGTGGTTACATGGCTCCTGAGTACGCATCCTTGGGAATTTTTGGAGTAAAATCTGATGTCTTTAGTTTTGGAGTGGCAATGCTTCAAATTCTAAGTGGTAGAAGTAGAAGATATTCTGACGTCTCCAAAACTCTTGCCTGGGATCTACCAGTCTATGCATGGGAGTTGTGGAAAAAAGGTGCAGGGCTAGAACTAATGGATCCAATGCTAGGCAATTCTTGTATTAACAAAGATCAATTCTTAAGATGCTTGCATGTTGGTCTACTATGTGTGGGGGCAAATGCAGATGATCGGCCAACCATGTCAGATGTGATATCTATGTTGACAATTAAAAGTGTGCAATTACCTCTGCCAAAAAGGCCAGGATATGGTATAGGAAATGAATCAGAAATTTCATCAACAGATGACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

571

Amino Acids

63.4

Weight (kDa)

6.42

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 7 - 120 5e-09 Protein kinase domain
Pkinase PF00069 298 - 425 6.8e-20 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 300 - 423 3.8e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1425
AccB7I CCANNNNNTGG 1 cut(s) 1670
AccBSI CCGCTC 1 cut(s) 764
AccI GTMKAC 2 cut(s) 1236, 1564
AccII CGCG 1 cut(s) 542
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 4 cut(s) 19, 441, 540, 762
AclI AACGTT 1 cut(s) 1056
AclWI GGATC 7 cut(s) 315, 328, 358, 844, 1452, 1496, 1509
AcoI YGGCCR 4 cut(s) 52, 480, 876, 1595
AcsI RAATTY 6 cut(s) 344, 526, 1254, 1338, 1389, 1692
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 1422
AfaI GTAC 2 cut(s) 466, 1325
AfeI AGCGCT 1 cut(s) 90
AfiI CCNNNNNNNGG 5 cut(s) 636, 664, 833, 1511, 1670
AflII CTTAAG 1 cut(s) 1543
AflIII ACRYGT 3 cut(s) 701, 1301, 1710
AgsI TTSAA 4 cut(s) 9, 789, 1192, 1388
AjnI CCWGG 4 cut(s) 313, 364, 1439, 1663
AjuI GAANNNNNNNTTGG 4 cut(s) 223, 255, 481, 513
AluBI AGCT 6 cut(s) 208, 550, 858, 907, 968, 1164
AluI AGCT 6 cut(s) 208, 550, 858, 907, 968, 1164
Alw26I GTCTC 4 cut(s) 228, 805, 944, 1429
AlwI GGATC 7 cut(s) 315, 328, 358, 844, 1452, 1496, 1509
Ama87I CYCGRG 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 90
AoxI GGCC 7 cut(s) 52, 186, 480, 533, 876, 1595, 1661
ApeKI GCWGC 2 cut(s) 98, 550
ApoI RAATTY 6 cut(s) 344, 526, 1254, 1338, 1389, 1692
AseI ATTAAT 1 cut(s) 1103
Asp700I GAANNNNTTC 2 cut(s) 1187, 1414
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 3 cut(s) 45, 109, 187
AsuHPI GGTGA 2 cut(s) 1049, 1100
AsuII TTCGAA 1 cut(s) 513
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 2 cut(s) 45, 109
BalI TGGCCA 2 cut(s) 482, 878
BamHI GGATCC 2 cut(s) 320, 1501
BbvI GCAGC 2 cut(s) 85, 537
BccI CCATC 4 cut(s) 262, 472, 544, 668
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 4 cut(s) 315, 366, 1441, 1665
BciVI GTATCC 2 cut(s) 67, 694
BcoDI GTCTC 4 cut(s) 228, 805, 944, 1429
BfaI CTAG 7 cut(s) 212, 407, 828, 1172, 1284, 1490, 1511
BfmI CTRYAG 1 cut(s) 1032
BfoI RGCGCY 1 cut(s) 92
BfrI CTTAAG 1 cut(s) 1543
BfuI GTATCC 2 cut(s) 67, 694
BglI GCCNNNNNGGC 1 cut(s) 1660
BisI GCNGC 3 cut(s) 99, 551, 762
BlsI GCNGC 3 cut(s) 100, 552, 763
Bme1390I CCNGG 4 cut(s) 315, 366, 1441, 1665
Bme18I GGWCC 2 cut(s) 45, 109
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 3 cut(s) 45, 109, 187
BmiI GGNNCC 5 cut(s) 46, 322, 522, 1317, 1503
BmrFI CCNGG 4 cut(s) 315, 366, 1441, 1665
BmrI ACTGGG 1 cut(s) 582
BmsI GCATC 5 cut(s) 431, 568, 872, 1337, 1538
BmuI ACTGGG 1 cut(s) 582
BpmI CTGGAG 2 cut(s) 387, 1225
Bpu14I TTCGAA 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 1197
BsaAI YACGTR 1 cut(s) 1234
BsaHI GRCGYC 1 cut(s) 1422
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 5 cut(s) 313, 483, 497, 1332, 1440
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 4 cut(s) 808, 838, 1223, 1253
Bsc4I CCNNNNNNNGG 5 cut(s) 636, 664, 833, 1511, 1670
Bse1I ACTGG 4 cut(s) 577, 675, 958, 1451
Bse3DI GCAATG 3 cut(s) 93, 1290, 1386
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 4 cut(s) 315, 366, 1441, 1665
BseDI CCNNGG 5 cut(s) 313, 483, 497, 1332, 1440
BseLI CCNNNNNNNGG 5 cut(s) 636, 664, 833, 1511, 1670
BseMI GCAATG 3 cut(s) 93, 1290, 1386
BseMII CTCAG 6 cut(s) 156, 537, 605, 885, 1016, 1311
BseNI ACTGG 4 cut(s) 577, 675, 958, 1451
BseXI GCAGC 2 cut(s) 85, 537
BseYI CCCAGC 1 cut(s) 1295
BsgI GTGCAG 1 cut(s) 1503
Bsh1236I CGCG 1 cut(s) 542
BshFI GGCC 7 cut(s) 54, 188, 482, 535, 878, 1597, 1663
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 84
BslFI GGGAC 3 cut(s) 58, 296, 1312
BslI CCNNNNNNNGG 5 cut(s) 636, 664, 833, 1511, 1670
BsmAI GTCTC 4 cut(s) 228, 805, 944, 1429
BsmBI CGTCTC 1 cut(s) 1429
BsmFI GGGAC 3 cut(s) 58, 296, 1312
BsmI GAATGC 1 cut(s) 1080
BsnI GGCC 7 cut(s) 54, 188, 482, 535, 878, 1597, 1663
Bso31I GGTCTC 1 cut(s) 805
BsoBI CYCGRG 1 cut(s) 338
Bsp119I TTCGAA 1 cut(s) 513
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 4 cut(s) 19, 441, 540, 762
BspANI GGCC 7 cut(s) 54, 188, 482, 535, 878, 1597, 1663
BspCNI CTCAG 6 cut(s) 157, 538, 604, 886, 1017, 1312
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 542
BspHI TCATGA 1 cut(s) 643
BspLI GGNNCC 5 cut(s) 46, 322, 522, 1317, 1503
BspPI GGATC 7 cut(s) 315, 328, 358, 844, 1452, 1496, 1509
BspT104I TTCGAA 1 cut(s) 513
BspTI CTTAAG 1 cut(s) 1543
BspTNI GGTCTC 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 764
BsrDI GCAATG 3 cut(s) 93, 1290, 1386
BsrI ACTGG 4 cut(s) 577, 675, 958, 1451
BssECI CCNNGG 5 cut(s) 313, 483, 497, 1332, 1440
BssMI GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
BssNAI GTATAC 1 cut(s) 1237
BssNI GRCGYC 1 cut(s) 1422
BssT1I CCWWGG 3 cut(s) 483, 497, 1332
Bst1107I GTATAC 1 cut(s) 1237
Bst2UI CCWGG 4 cut(s) 315, 366, 1441, 1665
Bst4CI ACNGT 2 cut(s) 289, 469
BstACI GRCGYC 1 cut(s) 1422
BstAFI CTTAAG 1 cut(s) 1543
BstBAI YACGTR 1 cut(s) 1234
BstBI TTCGAA 1 cut(s) 513
BstC8I GCNNGC 2 cut(s) 1166, 1553
BstDEI CTNAG 8 cut(s) 165, 183, 546, 591, 894, 1025, 1320, 1394
BstDSI CCRYGG 1 cut(s) 483
BstFNI CGCG 1 cut(s) 542
BstH2I RGCGCY 1 cut(s) 92
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 8 cut(s) 36, 323, 353, 839, 1447, 1504, 1537, 1594
BstMAI GTCTC 4 cut(s) 228, 805, 944, 1429
BstMBI GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
BstMWI GCNNNNNNNGC 6 cut(s) 95, 556, 729, 767, 855, 1660
BstNI CCWGG 4 cut(s) 315, 366, 1441, 1665
BstNSI RCATGY 5 cut(s) 705, 885, 1305, 1559, 1714
BstSCI CCNGG 4 cut(s) 313, 364, 1439, 1663
BstSFI CTRYAG 1 cut(s) 1032
BstUI CGCG 1 cut(s) 542
BstV1I GCAGC 2 cut(s) 85, 537
BstX2I RGATCY 5 cut(s) 320, 350, 836, 1444, 1501
BstYI RGATCY 5 cut(s) 320, 350, 836, 1444, 1501
BstZ17I GTATAC 1 cut(s) 1237
BsuI GTATCC 2 cut(s) 67, 694
BsuRI GGCC 7 cut(s) 54, 188, 482, 535, 878, 1597, 1663
BtgI CCRYGG 1 cut(s) 483
Cac8I GCNNGC 2 cut(s) 1166, 1553
CciI TCATGA 1 cut(s) 643
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 45, 109, 187
CseI GACGC 3 cut(s) 405, 531, 1296
Csp6I GTAC 2 cut(s) 465, 1324
CviQI GTAC 2 cut(s) 465, 1324
DdeI CTNAG 8 cut(s) 165, 183, 546, 591, 894, 1025, 1320, 1394
DpnI GATC 8 cut(s) 35, 322, 352, 838, 1446, 1503, 1536, 1593
DpnII GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
EaeI YGGCCR 4 cut(s) 52, 480, 876, 1595
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 483, 497, 1332
Eco31I GGTCTC 1 cut(s) 805
Eco32I GATATC 1 cut(s) 1617
Eco47I GGWCC 2 cut(s) 45, 109
Eco47III AGCGCT 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 2 cut(s) 45, 187
EcoRI GAATTC 1 cut(s) 1254
EcoRII CCWGG 4 cut(s) 313, 364, 1439, 1663
EcoRV GATATC 1 cut(s) 1617
EcoT14I CCWWGG 3 cut(s) 483, 497, 1332
EcoT22I ATGCAT 2 cut(s) 865, 1462
ErhI CCWWGG 3 cut(s) 483, 497, 1332
Esp3I CGTCTC 1 cut(s) 1429
FaqI GGGAC 3 cut(s) 58, 296, 1312
FblI GTMKAC 2 cut(s) 1236, 1564
Fnu4HI GCNGC 3 cut(s) 99, 551, 762
Fsp4HI GCNGC 3 cut(s) 99, 551, 762
FspBI CTAG 7 cut(s) 212, 407, 828, 1172, 1284, 1490, 1511
GlaI GCGC 1 cut(s) 90
GluI GCNGC 3 cut(s) 99, 551, 762
GsaI CCCAGC 1 cut(s) 1299
GsuI CTGGAG 2 cut(s) 387, 1225
HaeII RGCGCY 1 cut(s) 92
HaeIII GGCC 7 cut(s) 54, 188, 482, 535, 878, 1597, 1663
HapII CCGG 1 cut(s) 84
HgaI GACGC 3 cut(s) 405, 531, 1296
HhaI GCGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 1422
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 2 cut(s) 436, 1626
HindII GTYRAC 2 cut(s) 436, 1626
HindIII AAGCTT 1 cut(s) 1162
HinfI GANTC 8 cut(s) 80, 437, 692, 746, 942, 1151, 1188, 1685
HpaII CCGG 1 cut(s) 84
HphI GGTGA 2 cut(s) 1049, 1100
Hpy166II GTNNAC 7 cut(s) 61, 436, 1040, 1225, 1237, 1565, 1626
Hpy188III TCNNGA 7 cut(s) 84, 212, 338, 340, 644, 1242, 1319
Hpy8I GTNNAC 7 cut(s) 61, 436, 1040, 1225, 1237, 1565, 1626
HpyAV CCTTC 2 cut(s) 16, 668
HpyCH4III ACNGT 2 cut(s) 289, 469
HpyCH4IV ACGT 3 cut(s) 1056, 1233, 1422
HpyCH4V TGCA 9 cut(s) 863, 885, 1080, 1231, 1460, 1484, 1555, 1586, 1642
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 556, 729, 767, 855, 1660
HpyF3I CTNAG 8 cut(s) 165, 183, 546, 591, 894, 1025, 1320, 1394
HpySE526I ACGT 3 cut(s) 1056, 1233, 1422
Hsp92I GRCGYC 1 cut(s) 1422
HspAI GCGC 1 cut(s) 89
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
LmnI GCTCC 5 cut(s) 86, 205, 368, 729, 1321
Lsp1109I GCAGC 2 cut(s) 85, 537
LweI GCATC 5 cut(s) 431, 568, 872, 1337, 1538
MaeI CTAG 7 cut(s) 212, 407, 828, 1172, 1284, 1490, 1511
MaeII ACGT 3 cut(s) 1056, 1233, 1422
MaeIII GTNAC 2 cut(s) 289, 1307
MalI GATC 8 cut(s) 35, 322, 352, 838, 1446, 1503, 1536, 1593
MbiI CCGCTC 1 cut(s) 764
MboI GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
MboII GAAGA 3 cut(s) 209, 722, 1422
MflI RGATCY 5 cut(s) 320, 350, 836, 1444, 1501
MlsI TGGCCA 2 cut(s) 482, 878
MluNI TGGCCA 2 cut(s) 482, 878
MlyI GAGTC 3 cut(s) 431, 701, 740
MmeI TCCRAC 4 cut(s) 282, 439, 768, 1069
Mox20I TGGCCA 2 cut(s) 482, 878
Mph1103I ATGCAT 2 cut(s) 865, 1462
MroI TCCGGA 1 cut(s) 83
MroXI GAANNNNTTC 2 cut(s) 1187, 1414
MscI TGGCCA 2 cut(s) 482, 878
MslI CAYNNNNRTG 2 cut(s) 68, 1089
Msp20I TGGCCA 2 cut(s) 482, 878
MspA1I CMGCKG 1 cut(s) 443
MspCI CTTAAG 1 cut(s) 1543
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 4 cut(s) 315, 366, 1441, 1665
Mva1269I GAATGC 1 cut(s) 1080
MvaI CCWGG 4 cut(s) 315, 366, 1441, 1665
MvnI CGCG 1 cut(s) 542
MwoI GCNNNNNNNGC 6 cut(s) 95, 556, 729, 767, 855, 1660
NcoI CCATGG 1 cut(s) 483
NdeII GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
NlaIV GGNNCC 5 cut(s) 46, 322, 522, 1317, 1503
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 2 cut(s) 865, 1462
NspI RCATGY 5 cut(s) 705, 885, 1305, 1559, 1714
NspV TTCGAA 1 cut(s) 513
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 1 cut(s) 643
PciI ACATGT 3 cut(s) 701, 1301, 1710
PctI GAATGC 1 cut(s) 1080
PdmI GAANNNNTTC 2 cut(s) 1187, 1414
PfeI GAWTC 5 cut(s) 80, 942, 1151, 1188, 1685
PflMI CCANNNNNTGG 1 cut(s) 1670
PfoI TCCNGGA 1 cut(s) 364
PkrI GCNGC 3 cut(s) 100, 552, 763
PleI GAGTC 3 cut(s) 431, 700, 740
PpsI GAGTC 3 cut(s) 431, 700, 740
Ppu21I YACGTR 1 cut(s) 1234
PpuMI RGGWCCY 1 cut(s) 45
PscI ACATGT 3 cut(s) 701, 1301, 1710
PshBI ATTAAT 1 cut(s) 1103
Psp1406I AACGTT 1 cut(s) 1056
Psp5II RGGWCCY 1 cut(s) 45
Psp6I CCWGG 4 cut(s) 313, 364, 1439, 1663
PspFI CCCAGC 1 cut(s) 1295
PspGI CCWGG 4 cut(s) 313, 364, 1439, 1663
PspN4I GGNNCC 5 cut(s) 46, 322, 522, 1317, 1503
PspPI GGNCC 3 cut(s) 45, 109, 187
PspPPI RGGWCCY 1 cut(s) 45
PsuI RGATCY 5 cut(s) 320, 350, 836, 1444, 1501
RsaI GTAC 2 cut(s) 466, 1325
RsaNI GTAC 2 cut(s) 465, 1324
RseI CAYNNNNRTG 2 cut(s) 68, 1089
SatI GCNGC 3 cut(s) 99, 551, 762
Sau3AI GATC 8 cut(s) 33, 320, 350, 836, 1444, 1501, 1534, 1591
Sau96I GGNCC 3 cut(s) 45, 109, 187
SchI GAGTC 3 cut(s) 431, 701, 740
ScrFI CCNGG 4 cut(s) 315, 366, 1441, 1665
SfaNI GCATC 5 cut(s) 431, 568, 872, 1337, 1538
SfcI CTRYAG 1 cut(s) 1032
Sfr274I CTCGAG 1 cut(s) 338
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 2 cut(s) 45, 109
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 68, 1089
SmlI CTYRAG 3 cut(s) 338, 1212, 1543
SmoI CTYRAG 3 cut(s) 338, 1212, 1543
SsiI CCGC 4 cut(s) 19, 441, 540, 762
SspI AATATT 2 cut(s) 792, 1101
SspMI CTAG 7 cut(s) 212, 407, 828, 1172, 1284, 1490, 1511
StyD4I CCNGG 4 cut(s) 313, 364, 1439, 1663
StyI CCWWGG 3 cut(s) 483, 497, 1332
TaaI ACNGT 2 cut(s) 289, 469
TaiI ACGT 3 cut(s) 1059, 1236, 1425
TaqI TCGA 2 cut(s) 339, 513
TatI WGTACW 1 cut(s) 464
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 5 cut(s) 80, 942, 1151, 1188, 1685
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 2 cut(s) 98, 550
Tsp45I GTSAC 1 cut(s) 289
TspGWI ACGGA 1 cut(s) 170
Van91I CCANNNNNTGG 1 cut(s) 1670
Vha464I CTTAAG 1 cut(s) 1543
VpaK11BI GGWCC 2 cut(s) 45, 109
VspI ATTAAT 1 cut(s) 1103
XapI RAATTY 6 cut(s) 344, 526, 1254, 1338, 1389, 1692
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 5 cut(s) 705, 885, 1305, 1559, 1714
XcmI CCANNNNNNNNNTGG 2 cut(s) 667, 1458
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 2 cut(s) 1236, 1564
XmnI GAANNNNTTC 2 cut(s) 1187, 1414
XspI CTAG 7 cut(s) 212, 407, 828, 1172, 1284, 1490, 1511
ZraI GACGTC 1 cut(s) 1423
Zsp2I ATGCAT 2 cut(s) 865, 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.