Rroxscaffold_1G00054820

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000922
Physical Location & Seq
Forward (+)
76200262 .. 76203304
3043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054820.1

Sequence Viewer

Length: 858 bp
ATGTCTTCAATCCCCAGAGACGAAGCAACCTCGATTCAATGGCGGAAGGGCGATTTGATCGGTTCCGGGGCTTTTGGCCGTGTTTACATGGGCATGAATACTAAATCCGGAGAGCTTATTGCTGTAAAAGAGGCTCATACCAAGGTGCTTCAAGAAGAAGTGAGGCTTTTAGAAAACCTGTCTCATCCAAAAATTGTTAGATATTTAGGAACCGAGAGGGAGGATGAAACCTTGACTATGCTGTTGGAATATGTACCTGGTGGATCCATACAAGCACTATTGGAGAAATTTGGATCGTTTTCTGTTCCTGTAATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTCGAATATTTACACGAAAAAGGAATCATACATGGGGACATTAAGGGAGCAAATATCCTTGTTGATAATAAAGGGCGCATCAAACTAGCAGATTTTGGTACATCCAAACAGGCAGCCATTTCAGGTGCAAAGCCTACGAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTTGTGGGACTGGTCATGACTTTTCTGCAGATATATGGAGCGTTGGATGCACCGTGATTGAGATGGCCACTGGAAATCCTCCTTGGAACCAACAGTTTGAAACGGTTGCTACTTTCTTTTATTGTGTAGGGACAAGCAAGTCTCATCCACCAATCCCTGAGCATCTTTCTCCTGCAGCAAGAGATTTTCTGCACGAATGTCTGCAAAAGGAACCAACTGTAAGGCCATCCGCATCTGAGTTGCTTAAGCATCCTTTCTTAACTGGGAAAGAAGTGGAATCTCAGAATTCTGCCGTCATGGTTGGTATTGAATGTGGTCATATAATTTGTTATGTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

285

Amino Acids

31.4

Weight (kDa)

6.37

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 259 2.8e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 255 2.5e-42 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 49 - 152 9.8e-08 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 655
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 2 cut(s) 43, 747
AclWI GGATC 3 cut(s) 258, 271, 301
AcoI YGGCCR 2 cut(s) 76, 582
AcsI RAATTY 2 cut(s) 287, 802
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 3 cut(s) 255, 445, 490
AfiI CCNNNNNNNGG 1 cut(s) 485
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 5 cut(s) 9, 38, 152, 617, 827
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Alw26I GTCTC 3 cut(s) 12, 186, 663
AlwI GGATC 3 cut(s) 258, 271, 301
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 3 cut(s) 76, 582, 740
ApeKI GCWGC 2 cut(s) 458, 692
ApoI RAATTY 2 cut(s) 287, 802
AspLEI GCGC 1 cut(s) 423
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 316
BalI TGGCCA 1 cut(s) 584
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 470, 704
BccI CCATC 3 cut(s) 496, 574, 751
BceAI ACGGC 2 cut(s) 63, 794
BciT130I CCWGG 1 cut(s) 258
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 12, 186, 663
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 2 cut(s) 543, 690
BfrI CTTAAG 1 cut(s) 761
BisI GCNGC 2 cut(s) 459, 693
BlsI GCNGC 2 cut(s) 460, 694
Bme1390I CCNGG 2 cut(s) 67, 258
BmiI GGNNCC 6 cut(s) 64, 211, 265, 507, 605, 729
BmrFI CCNGG 2 cut(s) 67, 258
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 5 cut(s) 432, 554, 688, 758, 775
BmuI ACTGGG 1 cut(s) 789
Bpu10I CCTNAGC 1 cut(s) 675
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 66, 141, 599
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse1I ACTGG 4 cut(s) 503, 532, 592, 784
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 66, 141, 599
BseGI GGATG 8 cut(s) 184, 229, 446, 507, 569, 661, 743, 766
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 3 cut(s) 666, 744, 812
BseNI ACTGG 4 cut(s) 503, 532, 592, 784
BseXI GCAGC 2 cut(s) 470, 704
BsgI GTGCAG 1 cut(s) 692
BshFI GGCC 3 cut(s) 78, 584, 742
BsiSI CCGG 2 cut(s) 66, 108
BslFI GGGAC 3 cut(s) 395, 538, 661
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 3 cut(s) 12, 186, 663
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 395, 538, 661
BsnI GGCC 3 cut(s) 78, 584, 742
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 3 cut(s) 57, 263, 293
BspACI CCGC 2 cut(s) 43, 747
BspANI GGCC 3 cut(s) 78, 584, 742
BspCNI CTCAG 3 cut(s) 667, 745, 811
BspEI TCCGGA 1 cut(s) 107
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 6 cut(s) 64, 211, 265, 507, 605, 729
BspMAI CTGCAG 2 cut(s) 547, 694
BspPI GGATC 3 cut(s) 258, 271, 301
BspTI CTTAAG 1 cut(s) 761
BsrI ACTGG 4 cut(s) 503, 532, 592, 784
BssECI CCNNGG 3 cut(s) 66, 141, 599
BssMI GATC 3 cut(s) 57, 263, 293
BssT1I CCWWGG 2 cut(s) 141, 599
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 4 cut(s) 571, 612, 622, 736
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 675, 753, 798
BstF5I GGATG 8 cut(s) 184, 229, 446, 507, 569, 661, 743, 766
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 3 cut(s) 60, 266, 296
BstMAI GTCTC 3 cut(s) 12, 186, 663
BstMBI GATC 3 cut(s) 57, 263, 293
BstMWI GCNNNNNNNGC 2 cut(s) 337, 564
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 65, 256
BstSFI CTRYAG 2 cut(s) 543, 690
BstV1I GCAGC 2 cut(s) 470, 704
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BsuRI GGCC 3 cut(s) 78, 584, 742
BtsCI GGATG 8 cut(s) 184, 229, 446, 507, 569, 661, 743, 766
BtsIMutI CAGTG 1 cut(s) 585
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 1 cut(s) 532
CfoI GCGC 1 cut(s) 423
CsiI ACCWGGT 1 cut(s) 256
Csp6I GTAC 3 cut(s) 254, 444, 489
CviAII CATG 5 cut(s) 88, 94, 377, 533, 814
CviQI GTAC 3 cut(s) 254, 444, 489
DdeI CTNAG 3 cut(s) 675, 753, 798
DpnI GATC 3 cut(s) 59, 265, 295
DpnII GATC 3 cut(s) 57, 263, 293
DrdI GACNNNNNNGTC 1 cut(s) 655
DseDI GACNNNNNNGTC 1 cut(s) 655
EaeI YGGCCR 2 cut(s) 76, 582
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 2 cut(s) 141, 599
Eco57I CTGAAG 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 802
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 141, 599
ErhI CCWWGG 2 cut(s) 141, 599
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 5 cut(s) 91, 97, 380, 536, 817
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 3 cut(s) 395, 538, 661
FatI CATG 5 cut(s) 87, 93, 376, 532, 813
Fnu4HI GCNGC 2 cut(s) 459, 693
FokI GGATG 8 cut(s) 171, 236, 433, 514, 576, 648, 730, 753
Fsp4HI GCNGC 2 cut(s) 459, 693
FspBI CTAG 1 cut(s) 431
GlaI GCGC 1 cut(s) 422
GluI GCNGC 2 cut(s) 459, 693
HaeIII GGCC 3 cut(s) 78, 584, 742
HapII CCGG 2 cut(s) 66, 108
HhaI GCGC 1 cut(s) 423
Hin1II CATG 5 cut(s) 91, 97, 380, 536, 817
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HinfI GANTC 4 cut(s) 34, 321, 369, 794
HpaII CCGG 2 cut(s) 66, 108
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 85, 491
Hpy188I TCNGA 2 cut(s) 754, 801
Hpy188III TCNNGA 4 cut(s) 108, 152, 509, 533
Hpy8I GTNNAC 2 cut(s) 85, 491
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 4 cut(s) 571, 612, 622, 736
HpyCH4V TGCA 6 cut(s) 473, 545, 567, 692, 709, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 564
HpyF3I CTNAG 3 cut(s) 675, 753, 798
Hsp92II CATG 5 cut(s) 91, 97, 380, 536, 817
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 107
Kzo9I GATC 3 cut(s) 57, 263, 293
LmnI GCTCC 3 cut(s) 392, 511, 555
Lsp1109I GCAGC 2 cut(s) 470, 704
LweI GCATC 5 cut(s) 432, 554, 688, 758, 775
MabI ACCWGGT 1 cut(s) 256
MaeI CTAG 1 cut(s) 431
MalI GATC 3 cut(s) 59, 265, 295
MboI GATC 3 cut(s) 57, 263, 293
MboII GAAGA 1 cut(s) 167
MflI RGATCY 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 4 cut(s) 192, 287, 802, 840
MluNI TGGCCA 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 225, 541
MnlI CCTC 7 cut(s) 40, 124, 156, 210, 214, 477, 606
Mox20I TGGCCA 1 cut(s) 584
MroI TCCGGA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 584
MseI TTAA 3 cut(s) 387, 762, 776
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 584
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 2 cut(s) 66, 108
MspR9I CCNGG 2 cut(s) 67, 258
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 2 cut(s) 337, 564
NciI CCSGG 1 cut(s) 67
NdeII GATC 3 cut(s) 57, 263, 293
NlaIII CATG 5 cut(s) 91, 97, 380, 536, 817
NlaIV GGNNCC 6 cut(s) 64, 211, 265, 507, 605, 729
PagI TCATGA 1 cut(s) 532
PfeI GAWTC 4 cut(s) 34, 321, 369, 794
PkrI GCNGC 2 cut(s) 460, 694
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 6 cut(s) 64, 211, 265, 507, 605, 729
PstI CTGCAG 2 cut(s) 547, 694
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 3 cut(s) 255, 445, 490
RsaNI GTAC 3 cut(s) 254, 444, 489
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 3 cut(s) 387, 762, 776
SatI GCNGC 2 cut(s) 459, 693
Sau3AI GATC 3 cut(s) 57, 263, 293
ScrFI CCNGG 2 cut(s) 67, 258
SetI ASST 7 cut(s) 32, 117, 147, 180, 233, 259, 472
SexAI ACCWGGT 1 cut(s) 256
SfaNI GCATC 5 cut(s) 432, 554, 688, 758, 775
SfcI CTRYAG 2 cut(s) 543, 690
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 4 cut(s) 192, 287, 802, 840
SsiI CCGC 2 cut(s) 43, 747
SspI AATATT 1 cut(s) 353
SspMI CTAG 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 65, 256
StyI CCWWGG 2 cut(s) 141, 599
TaaI ACNGT 4 cut(s) 571, 612, 622, 736
TaqI TCGA 2 cut(s) 32, 348
TasI AATT 4 cut(s) 192, 287, 802, 840
TfiI GAWTC 4 cut(s) 34, 321, 369, 794
Tru1I TTAA 3 cut(s) 387, 762, 776
Tru9I TTAA 3 cut(s) 387, 762, 776
TscAI CASTG 1 cut(s) 592
TseI GCWGC 2 cut(s) 458, 692
TspDTI ATGAA 2 cut(s) 110, 240
TspRI CASTG 1 cut(s) 592
Vha464I CTTAAG 1 cut(s) 761
XapI RAATTY 2 cut(s) 287, 802
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.