Rmu_sc0005861.1_g000005

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005861.1
OG0000922
Physical Location & Seq
Reverse (-)
12842 .. 18987
6146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005861.1_g000005.1.cds

Sequence Viewer

Length: 1989 bp
atggcaatctcgccttatggcgcaaggcttctcacaaaacgccctggaatcgactacgatgagacatattttctcgtaatggatgacattgcactccactatcctgtcagtttggctcatacgaaggtgcttcaagatgaagtgaggcttttagaaaacctgtcgcatccgaaaattattagatgtttaggaaccgagagggaggatgaaaccttaactatgctgttggaatatgtacctggaggatccatacaagcactattggagaaatttggatctttctctgttcctgtaataaaaagattcaccaagcagttgttgctggcactagaatatttacacaaaaaaggaatcatacatggggacattaagggagcaaatatccttgttgataataaagggcgcattaaactagcagattttgccgtttcaggtgcaaagcctacaaggggtacaccatactggatggctcctgaagtcatttgtgggactgggacaagcaagtctcatccaccaatccctgatcatctttctcctgcagcaaaagattttctgcacgaatgtctgcaaaaggaaccaactgtaaggccatccgcgtctgagttgcttaagcatccttttttaactgggaaagaagtggaatctcagaattctgccgtcatggaagtagaagtagcatcctcattttgccaaacaaattctgacgaaattgatatcatgtgggacgatgatgcaaactccaattttgtatccaatccagctagtggcggaactagaaaattcattgtgcagggagccattaaacaagtgttttcaaaaaattttaaattctttaagaggtcgagaagaactacaagaaatggaccagcgaagggtgagttcagctttgcatctatcaaggatgccacacacaacttttctgaggaaaatttgattggacaagggggatttggacctgtttataagggaaaattggtgaccggacaagaagtagctgtgaaacgcctttcatcatcttcacatcaaggagataaggagtttaagaatgaactgagactcatgcagaaactccaacatagaaacattattcagatattaggattttgcattcatggtggcggaagatgtataatatacgagtacatggcaaacacaagtttaaacaaccatttatttggttcatccagtgacttgctactagattggaataagcgtttcaacataattgaaggaattgctcaaggattggtttacttgcacaaatactccagattgagagtaattcatagagatataaaagccagtaatgttctactggatgcacaaatgaatcccaaaattgctgattttggtttagcaaaaatttttgcagatgatgaagagacggtaaaaattacagaaatttgtgggacaatcggttacatgccacccgagtacctcataacaggcatcctttccatgaaaaccgatgtctacagttatggagtgttaatgcttgaaatcataagtgatgatgaagagacagtaaaaattacggaaatttgtgggacattcggttacatgcctcccgagtacctcatggcaggcatcctttccatgaaaaccgatgtctacagttatggagtgttaatgcttgaaatcataagtggtaacaaaaatttgaatgctgacggcaattatttggtaaaagatgcatggcagttatggcaaaaaggtgcagggctacaactagtggatccaaaactggaagtttctcgtattaacaaacatcaactgttaagatgcatccatgttggtctgctgtgcgcggaagaaaatgaagatgaccggcccaccatgtcagatgcgatatctatgttgaaaaatgaagtgcctttgcctgaaccaacaatgccagcattttctagatacagtaatctgcctactactatgttcatagaatgttcatcaataaacagtatgacgatttctgactttgttggacgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

662

Amino Acids

73.93

Weight (kDa)

6.25

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 963
AasI GACNNNNNNGTC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 2 cut(s) 1473, 1611
AccII CGCG 2 cut(s) 596, 1808
AciI CCGC 4 cut(s) 594, 768, 1119, 1808
AclWI GGATC 5 cut(s) 240, 253, 283, 1730, 1743
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 5 cut(s) 237, 454, 1142, 1436, 1574
AfiI CCNNNNNNNGG 3 cut(s) 449, 764, 872
AflII CTTAAG 1 cut(s) 608
AgsI TTSAA 8 cut(s) 134, 816, 1219, 1229, 1499, 1637, 1663, 1861
AhlI ACTAGT 1 cut(s) 1729
AjnI CCWGG 2 cut(s) 43, 238
AjuI GAANNNNNNNTTGG 2 cut(s) 918, 950
AluBI AGCT 3 cut(s) 761, 885, 995
AluI AGCT 3 cut(s) 761, 885, 995
Alw26I GTCTC 5 cut(s) 56, 510, 1048, 1376, 1514
AlwI GGATC 5 cut(s) 240, 253, 283, 1730, 1743
Ama87I CYCGRG 2 cut(s) 1430, 1568
AoxI GGCC 2 cut(s) 587, 1829
ApeKI GCWGC 1 cut(s) 539
AspLEI GCGC 3 cut(s) 23, 405, 1808
AspS9I GGNCC 3 cut(s) 863, 953, 1830
AsuHPI GGTGA 3 cut(s) 298, 887, 988
AvaI CYCGRG 2 cut(s) 1430, 1568
AvaII GGWCC 2 cut(s) 863, 953
BaeI ACNNNNGTAYC 2 cut(s) 1418, 1451
BamHI GGATCC 2 cut(s) 245, 1735
BbvI GCAGC 1 cut(s) 551
BccI CCATC 2 cut(s) 460, 598
BceAI ACGGC 3 cut(s) 410, 641, 1687
BciT130I CCWGG 2 cut(s) 45, 240
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 5 cut(s) 56, 510, 1048, 1376, 1514
BcuI ACTAGT 1 cut(s) 1729
BfaI CTAG 7 cut(s) 329, 413, 762, 774, 1199, 1730, 1905
BfmI CTRYAG 3 cut(s) 537, 1474, 1612
BfrI CTTAAG 1 cut(s) 608
BfuI GTATCC 1 cut(s) 760
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 45, 240
Bme18I GGWCC 2 cut(s) 863, 953
BmeT110I CYCGRG 2 cut(s) 1430, 1568
BmgT120I GGNCC 3 cut(s) 863, 953, 1830
BmiI GGNNCC 6 cut(s) 193, 247, 471, 576, 796, 1737
BmrFI CCNGG 2 cut(s) 45, 240
BmrI ACTGGG 2 cut(s) 501, 636
BmuI ACTGGG 2 cut(s) 501, 636
BpmI CTGGAG 2 cut(s) 261, 1252
BpuEI CTTGAG 1 cut(s) 1224
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 980
BsaXI ACNNNNNCTCC 2 cut(s) 1250, 1280
Bsc4I CCNNNNNNNGG 3 cut(s) 449, 764, 872
Bse118I RCCGGY 1 cut(s) 1827
Bse1I ACTGG 7 cut(s) 467, 496, 631, 1185, 1302, 1320, 1749
Bse3DI GCAATG 1 cut(s) 87
BseBI CCWGG 2 cut(s) 45, 240
BseDI CCNNGG 1 cut(s) 43
BseLI CCNNNNNNNGG 3 cut(s) 449, 764, 872
BseMI GCAATG 1 cut(s) 87
BseMII CTCAG 4 cut(s) 591, 659, 912, 1043
BseNI ACTGG 7 cut(s) 467, 496, 631, 1185, 1302, 1320, 1749
BseXI GCAGC 1 cut(s) 551
BsgI GTGCAG 3 cut(s) 539, 809, 1737
Bsh1236I CGCG 2 cut(s) 596, 1808
BshFI GGCC 2 cut(s) 589, 1831
BsiHKCI CYCGRG 2 cut(s) 1430, 1568
BsiSI CCGG 2 cut(s) 981, 1828
BslFI GGGAC 6 cut(s) 377, 502, 508, 737, 1423, 1561
BslI CCNNNNNNNGG 3 cut(s) 449, 764, 872
BsmAI GTCTC 5 cut(s) 56, 510, 1048, 1376, 1514
BsmBI CGTCTC 1 cut(s) 1376
BsmFI GGGAC 6 cut(s) 377, 502, 508, 737, 1423, 1561
BsmI GAATGC 2 cut(s) 1107, 1669
BsnI GGCC 2 cut(s) 589, 1831
BsoBI CYCGRG 2 cut(s) 1430, 1568
Bsp143I GATC 4 cut(s) 245, 275, 523, 1735
BspACI CCGC 4 cut(s) 594, 768, 1119, 1808
BspANI GGCC 2 cut(s) 589, 1831
BspCNI CTCAG 4 cut(s) 592, 658, 913, 1044
BspFNI CGCG 2 cut(s) 596, 1808
BspLI GGNNCC 6 cut(s) 193, 247, 471, 576, 796, 1737
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 5 cut(s) 240, 253, 283, 1730, 1743
BspTI CTTAAG 1 cut(s) 608
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 1 cut(s) 1827
BsrI ACTGG 7 cut(s) 467, 496, 631, 1185, 1302, 1320, 1749
BssAI RCCGGY 1 cut(s) 1827
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 4 cut(s) 245, 275, 523, 1735
Bst2UI CCWGG 2 cut(s) 45, 240
Bst4CI ACNGT 8 cut(s) 583, 1387, 1478, 1525, 1616, 1776, 1913, 1958
Bst6I CTCTTC 2 cut(s) 1374, 1512
BstAFI CTTAAG 1 cut(s) 608
BstAPI GCANNNNNTGC 2 cut(s) 319, 422
BstC8I GCNNGC 3 cut(s) 324, 1585, 1896
BstDEI CTNAG 4 cut(s) 600, 645, 921, 1052
BstEII GGTNACC 1 cut(s) 976
BstFNI CGCG 2 cut(s) 596, 1808
BstHHI GCGC 3 cut(s) 23, 405, 1808
BstKTI GATC 4 cut(s) 248, 278, 526, 1738
BstMAI GTCTC 5 cut(s) 56, 510, 1048, 1376, 1514
BstMBI GATC 4 cut(s) 245, 275, 523, 1735
BstMWI GCNNNNNNNGC 3 cut(s) 319, 422, 1705
BstNI CCWGG 2 cut(s) 45, 240
BstNSI RCATGY 2 cut(s) 1426, 1564
BstPI GGTNACC 1 cut(s) 976
BstSCI CCNGG 2 cut(s) 43, 238
BstSFI CTRYAG 3 cut(s) 537, 1474, 1612
BstUI CGCG 2 cut(s) 596, 1808
BstV1I GCAGC 1 cut(s) 551
BstX2I RGATCY 3 cut(s) 245, 275, 1735
BstXI CCANNNNNNTGG 1 cut(s) 1175
BstYI RGATCY 3 cut(s) 245, 275, 1735
BsuI GTATCC 1 cut(s) 760
BsuRI GGCC 2 cut(s) 589, 1831
BtsIMutI CAGTG 1 cut(s) 1192
Cac8I GCNNGC 3 cut(s) 324, 1585, 1896
CfoI GCGC 3 cut(s) 23, 405, 1808
Cfr10I RCCGGY 1 cut(s) 1827
Cfr13I GGNCC 3 cut(s) 863, 953, 1830
CseI GACGC 1 cut(s) 585
Csp6I GTAC 5 cut(s) 236, 453, 1141, 1435, 1573
CviQI GTAC 5 cut(s) 236, 453, 1141, 1435, 1573
DdeI CTNAG 4 cut(s) 600, 645, 921, 1052
DpnI GATC 4 cut(s) 247, 277, 525, 1737
DpnII GATC 4 cut(s) 245, 275, 523, 1735
DraI TTTAAA 2 cut(s) 826, 1161
DrdI GACNNNNNNGTC 1 cut(s) 502
DseDI GACNNNNNNGTC 1 cut(s) 502
Eam1104I CTCTTC 2 cut(s) 1374, 1512
EarI CTCTTC 2 cut(s) 1374, 1512
EciI GGCGGA 2 cut(s) 783, 1134
Eco32I GATATC 2 cut(s) 715, 1851
Eco47I GGWCC 2 cut(s) 863, 953
Eco57I CTGAAG 1 cut(s) 495
Eco88I CYCGRG 2 cut(s) 1430, 1568
Eco91I GGTNACC 1 cut(s) 976
EcoO65I GGTNACC 1 cut(s) 976
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 43, 238
EcoRV GATATC 2 cut(s) 715, 1851
EcoT22I ATGCAT 2 cut(s) 1696, 1787
Esp3I CGTCTC 1 cut(s) 1376
FalI AAGNNNNNCTT 2 cut(s) 132, 164
FaqI GGGAC 6 cut(s) 377, 502, 508, 737, 1423, 1561
FbaI TGATCA 1 cut(s) 523
FblI GTMKAC 2 cut(s) 1473, 1611
Fnu4HI GCNGC 1 cut(s) 540
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 7 cut(s) 329, 413, 762, 774, 1199, 1730, 1905
GlaI GCGC 3 cut(s) 22, 404, 1807
GluI GCNGC 1 cut(s) 540
GsuI CTGGAG 2 cut(s) 261, 1252
HaeIII GGCC 2 cut(s) 589, 1831
HapII CCGG 2 cut(s) 981, 1828
HgaI GACGC 1 cut(s) 585
HhaI GCGC 3 cut(s) 23, 405, 1808
Hin6I GCGC 3 cut(s) 21, 403, 1806
HinP1I GCGC 3 cut(s) 21, 403, 1806
HinfI GANTC 6 cut(s) 48, 303, 351, 641, 1056, 1330
HpaII CCGG 2 cut(s) 981, 1828
HphI GGTGA 3 cut(s) 298, 887, 988
Hpy166II GTNNAC 4 cut(s) 455, 1252, 1474, 1612
Hpy188I TCNGA 8 cut(s) 171, 601, 648, 703, 922, 1092, 1843, 1972
Hpy188III TCNNGA 6 cut(s) 134, 473, 843, 1269, 1568, 1905
Hpy8I GTNNAC 4 cut(s) 455, 1252, 1474, 1612
HpyAV CCTTC 3 cut(s) 118, 865, 1223
HpyCH4III ACNGT 8 cut(s) 583, 1387, 1478, 1525, 1616, 1776, 1913, 1958
HpyCH4IV ACGT 1 cut(s) 1984
HpyF10VI GCNNNNNNNGC 3 cut(s) 319, 422, 1705
HpyF3I CTNAG 4 cut(s) 600, 645, 921, 1052
HpySE526I ACGT 1 cut(s) 1984
HspAI GCGC 3 cut(s) 21, 403, 1806
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 4 cut(s) 245, 275, 523, 1735
LmnI GCTCC 3 cut(s) 374, 475, 794
Lsp1109I GCAGC 1 cut(s) 551
MaeI CTAG 7 cut(s) 329, 413, 762, 774, 1199, 1730, 1905
MaeII ACGT 1 cut(s) 1984
MaeIII GTNAC 5 cut(s) 976, 1187, 1418, 1556, 1649
MalI GATC 4 cut(s) 247, 277, 525, 1737
MboI GATC 4 cut(s) 245, 275, 523, 1735
MboII GAAGA 7 cut(s) 858, 1008, 1134, 1391, 1529, 1823, 1832
MflI RGATCY 3 cut(s) 245, 275, 1735
MlyI GAGTC 1 cut(s) 1050
MmeI TCCRAC 3 cut(s) 207, 1096, 1960
Mph1103I ATGCAT 2 cut(s) 1696, 1787
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 2 cut(s) 981, 1828
MspR9I CCNGG 2 cut(s) 45, 240
MssI GTTTAAAC 1 cut(s) 1161
Mva1269I GAATGC 2 cut(s) 1107, 1669
MvaI CCWGG 2 cut(s) 45, 240
MvnI CGCG 2 cut(s) 596, 1808
MwoI GCNNNNNNNGC 3 cut(s) 319, 422, 1705
NdeII GATC 4 cut(s) 245, 275, 523, 1735
NlaIV GGNNCC 6 cut(s) 193, 247, 471, 576, 796, 1737
NmuCI GTSAC 2 cut(s) 976, 1187
NsiI ATGCAT 2 cut(s) 1696, 1787
NspI RCATGY 2 cut(s) 1426, 1564
PctI GAATGC 2 cut(s) 1107, 1669
PfeI GAWTC 5 cut(s) 48, 303, 351, 641, 1330
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 1 cut(s) 541
PleI GAGTC 1 cut(s) 1050
PmeI GTTTAAAC 1 cut(s) 1161
PpsI GAGTC 1 cut(s) 1050
PsiI TTATAA 1 cut(s) 963
Psp6I CCWGG 2 cut(s) 43, 238
PspEI GGTNACC 1 cut(s) 976
PspGI CCWGG 2 cut(s) 43, 238
PspN4I GGNNCC 6 cut(s) 193, 247, 471, 576, 796, 1737
PspPI GGNCC 3 cut(s) 863, 953, 1830
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 3 cut(s) 245, 275, 1735
RsaI GTAC 5 cut(s) 237, 454, 1142, 1436, 1574
RsaNI GTAC 5 cut(s) 236, 453, 1141, 1435, 1573
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 4 cut(s) 245, 275, 523, 1735
Sau96I GGNCC 3 cut(s) 863, 953, 1830
SchI GAGTC 1 cut(s) 1050
ScrFI CCNGG 2 cut(s) 45, 240
SfcI CTRYAG 3 cut(s) 537, 1474, 1612
SinI GGWCC 2 cut(s) 863, 953
SmlI CTYRAG 2 cut(s) 608, 1239
SmoI CTYRAG 2 cut(s) 608, 1239
SpeI ACTAGT 1 cut(s) 1729
SsiI CCGC 4 cut(s) 594, 768, 1119, 1808
SspI AATATT 1 cut(s) 335
SspMI CTAG 7 cut(s) 329, 413, 762, 774, 1199, 1730, 1905
StyD4I CCNGG 2 cut(s) 43, 238
TaaI ACNGT 8 cut(s) 583, 1387, 1478, 1525, 1616, 1776, 1913, 1958
TaiI ACGT 1 cut(s) 1987
TaqI TCGA 2 cut(s) 51, 842
TatI WGTACW 1 cut(s) 1140
TfiI GAWTC 5 cut(s) 48, 303, 351, 641, 1330
TscAI CASTG 1 cut(s) 1192
TseFI GTSAC 2 cut(s) 976, 1187
TseI GCWGC 1 cut(s) 539
Tsp45I GTSAC 2 cut(s) 976, 1187
TspGWI ACGGA 1 cut(s) 1550
TspRI CASTG 1 cut(s) 1192
Van91I CCANNNNNTGG 1 cut(s) 764
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 863, 953
XbaI TCTAGA 1 cut(s) 1904
XceI RCATGY 2 cut(s) 1426, 1564
XmiI GTMKAC 2 cut(s) 1473, 1611
XspI CTAG 7 cut(s) 329, 413, 762, 774, 1199, 1730, 1905
Zsp2I ATGCAT 2 cut(s) 1696, 1787
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.