Rroxscaffold_1G00054830

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000922
Physical Location & Seq
Forward (+)
76210614 .. 76212281
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00054830.1

Sequence Viewer

Length: 1200 bp
ATGGAACAGGAAGTAGTGGCCTCCTCATTTTGCCAAACAAATTCGGATGAAATTTACATCACGTTGGATGATGATGCAAACTCCGATTTTGTATCCGACCTAGCAAGTGTCGGAACTAGAAAATTGATTGTAGAGGGAGCCATTAAACAAGTGTTTTCAAAAATTCATAAATTCTTTAAGAGGTCGGGAAGAACTACAAGAAATAGATCGGCCAAGGGTGAGTTCAGCTATGCATCTATCATGGTGGCCACAGGCAACTTTTCTGAGGAAAATAAGATCGGACAAGGGGGATTTGGAGCTGTTTATAAGGGAAAATTGGTGACCGGACAAGAAGTGGCTGTGAAAAGGCTTTCAAAATATTCAAATCAAGGAAAATTGCAGTTTAAGAATGAACTGAGACTCATGCGTGAACTCCAACATAGAAACATTATTCGGATATTAGGGTTTTGCATTCATGTTGACGAAAGAGTTCTAATATATGAGTACATGGCAAACGCAAGTTCATCGAGTGACTTGCTACTAGATTGGAATAAGCGTTTCAACATAATTGAAGGAATTGCTCAAGGATTGGTTTACTTGCATAAATTCTCCAGATTGAGAGTAATTCATAGAGATGTAAAAGCTAGTAATGTTCTACTTGATGCACAAATGAATCCCAAAGTTGCTGATTTTGGTATAGCAAAGATTTGTGCAGATGATGAAGAGACAGTAGAAACTGCGGCAATTTGTGGGACAATGGGTTACATGGCTCCCGAAAACATCATAAATGGCATCCTTTCCGTGAAAACCGATGTCTACAGTTATGGAGTGTTAATGCTTGAAATCATAAGTGGTAACAAAAATTTCAAAGCTGACGGCAATTATTTGGTAAAAGATGCATGGCTGTTATGGCAAAAAGGTGCAGGGCTACAACTAGTGGATCCAAAACTGGAAGTTTCTCGTATTAACAAGCATCAACTGTTAAGATGTATCCATGTTGGTCTGCTGTGCGCGGAAGAAAATGAAGATGACCGGCCCACCATGTCAGATGCGATATCTATGTTGAAAAATGAAGTGCCTTTGCCTGAACCAAAAATGCCAGCATTTTCTAGATACAGTAATCTGACTACTGCTAGTATAGATGAAAATGGACCGGAAACTACGTCCATATATAGTTTATCAATAAACAGTATGACGATTTCCGACTTTGGTGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

399

Amino Acids

44.53

Weight (kDa)

6.42

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 88 - 288 4.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 90 - 343 7.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 306
AccI GTMKAC 1 cut(s) 795
AccII CGCG 1 cut(s) 992
AciI CCGC 2 cut(s) 719, 992
AclWI GGATC 2 cut(s) 914, 927
AcoI YGGCCR 2 cut(s) 210, 246
AcsI RAATTY 6 cut(s) 40, 51, 162, 170, 584, 841
AfaI GTAC 1 cut(s) 485
AgsI TTSAA 8 cut(s) 159, 354, 363, 541, 551, 821, 847, 1045
AhlI ACTAGT 1 cut(s) 913
AjuI GAANNNNNNNTTGG 2 cut(s) 206, 238
AluBI AGCT 4 cut(s) 228, 299, 623, 851
AluI AGCT 4 cut(s) 228, 299, 623, 851
Alw26I GTCTC 2 cut(s) 391, 698
AlwI GGATC 2 cut(s) 914, 927
AoxI GGCC 4 cut(s) 18, 210, 246, 1013
ApoI RAATTY 6 cut(s) 40, 51, 162, 170, 584, 841
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 992
AspS9I GGNCC 2 cut(s) 1014, 1130
AsuHPI GGTGA 2 cut(s) 230, 331
AvaII GGWCC 1 cut(s) 1130
BalI TGGCCA 1 cut(s) 248
BamHI GGATCC 1 cut(s) 919
BceAI ACGGC 1 cut(s) 871
BcgI CGANNNNNNTGC 4 cut(s) 486, 496, 520, 530
BciVI GTATCC 2 cut(s) 103, 980
BcoDI GTCTC 2 cut(s) 391, 698
BcuI ACTAGT 1 cut(s) 913
BfaI CTAG 7 cut(s) 101, 117, 521, 624, 914, 1089, 1113
BfmI CTRYAG 1 cut(s) 796
BfuI GTATCC 2 cut(s) 103, 980
BisI GCNGC 1 cut(s) 720
BlsI GCNGC 1 cut(s) 721
Bme18I GGWCC 1 cut(s) 1130
BmgT120I GGNCC 2 cut(s) 1014, 1130
BmiI GGNNCC 3 cut(s) 139, 750, 921
BmsI GCATC 7 cut(s) 64, 242, 631, 780, 865, 961, 1018
BpmI CTGGAG 1 cut(s) 574
BpuEI CTTGAG 1 cut(s) 546
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 2 cut(s) 323, 1132
Bse118I RCCGGY 1 cut(s) 1011
Bse1I ACTGG 1 cut(s) 933
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 3 cut(s) 52, 73, 771
BseMII CTCAG 2 cut(s) 255, 386
BseNI ACTGG 1 cut(s) 933
BseRI GAGGAG 1 cut(s) 13
BsgI GTGCAG 2 cut(s) 711, 921
Bsh1236I CGCG 1 cut(s) 992
BshFI GGCC 4 cut(s) 20, 212, 248, 1015
BsiSI CCGG 3 cut(s) 324, 1012, 1133
BslFI GGGAC 1 cut(s) 745
BsmAI GTCTC 2 cut(s) 391, 698
BsmFI GGGAC 1 cut(s) 745
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 4 cut(s) 20, 212, 248, 1015
Bsp143I GATC 3 cut(s) 206, 276, 919
BspACI CCGC 2 cut(s) 719, 992
BspANI GGCC 4 cut(s) 20, 212, 248, 1015
BspCNI CTCAG 2 cut(s) 256, 387
BspFNI CGCG 1 cut(s) 992
BspLI GGNNCC 3 cut(s) 139, 750, 921
BspPI GGATC 2 cut(s) 914, 927
BsrFI RCCGGY 1 cut(s) 1011
BsrI ACTGG 1 cut(s) 933
BssAI RCCGGY 1 cut(s) 1011
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 206, 276, 919
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 5 cut(s) 709, 800, 960, 1097, 1169
Bst6I CTCTTC 1 cut(s) 696
BstC8I GCNNGC 1 cut(s) 1080
BstDEI CTNAG 2 cut(s) 264, 395
BstEII GGTNACC 1 cut(s) 319
BstF5I GGATG 3 cut(s) 52, 73, 771
BstFNI CGCG 1 cut(s) 992
BstHHI GCGC 1 cut(s) 992
BstKTI GATC 3 cut(s) 209, 279, 922
BstMAI GTCTC 2 cut(s) 391, 698
BstMBI GATC 3 cut(s) 206, 276, 919
BstMWI GCNNNNNNNGC 1 cut(s) 889
BstPI GGTNACC 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 796
BstUI CGCG 1 cut(s) 992
BstX2I RGATCY 1 cut(s) 919
BstYI RGATCY 1 cut(s) 919
BsuI GTATCC 2 cut(s) 103, 980
BsuRI GGCC 4 cut(s) 20, 212, 248, 1015
BtsCI GGATG 3 cut(s) 52, 73, 771
Cac8I GCNNGC 1 cut(s) 1080
CfoI GCGC 1 cut(s) 992
Cfr10I RCCGGY 1 cut(s) 1011
Cfr13I GGNCC 2 cut(s) 1014, 1130
Csp6I GTAC 1 cut(s) 484
CviAII CATG 8 cut(s) 241, 403, 455, 487, 745, 879, 974, 1021
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 2 cut(s) 264, 395
DpnI GATC 3 cut(s) 208, 278, 921
DpnII GATC 3 cut(s) 206, 276, 919
EaeI YGGCCR 2 cut(s) 210, 246
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 213
Eco32I GATATC 1 cut(s) 1035
Eco47I GGWCC 1 cut(s) 1130
Eco91I GGTNACC 1 cut(s) 319
EcoO65I GGTNACC 1 cut(s) 319
EcoRV GATATC 1 cut(s) 1035
EcoT14I CCWWGG 1 cut(s) 213
EcoT22I ATGCAT 2 cut(s) 235, 880
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 8 cut(s) 244, 406, 458, 490, 748, 882, 977, 1024
FaqI GGGAC 1 cut(s) 745
FatI CATG 8 cut(s) 240, 402, 454, 486, 744, 878, 973, 1020
FblI GTMKAC 1 cut(s) 795
Fnu4HI GCNGC 1 cut(s) 720
FokI GGATG 3 cut(s) 59, 80, 758
Fsp4HI GCNGC 1 cut(s) 720
FspBI CTAG 7 cut(s) 101, 117, 521, 624, 914, 1089, 1113
GlaI GCGC 1 cut(s) 991
GluI GCNGC 1 cut(s) 720
GsuI CTGGAG 1 cut(s) 574
HaeIII GGCC 4 cut(s) 20, 212, 248, 1015
HapII CCGG 3 cut(s) 324, 1012, 1133
HhaI GCGC 1 cut(s) 992
Hin1II CATG 8 cut(s) 244, 406, 458, 490, 748, 882, 977, 1024
Hin6I GCGC 1 cut(s) 990
HinP1I GCGC 1 cut(s) 990
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HinfI GANTC 2 cut(s) 399, 652
HpaII CCGG 3 cut(s) 324, 1012, 1133
HphI GGTGA 2 cut(s) 230, 331
Hpy166II GTNNAC 5 cut(s) 410, 460, 574, 796, 1193
Hpy188III TCNNGA 4 cut(s) 186, 591, 752, 1089
Hpy8I GTNNAC 5 cut(s) 410, 460, 574, 796, 1193
HpyAV CCTTC 1 cut(s) 545
HpyCH4III ACNGT 5 cut(s) 709, 800, 960, 1097, 1169
HpyCH4IV ACGT 3 cut(s) 62, 1142, 1195
HpyCH4V TGCA 9 cut(s) 77, 233, 379, 450, 580, 644, 692, 878, 902
HpyF10VI GCNNNNNNNGC 1 cut(s) 889
HpyF3I CTNAG 2 cut(s) 264, 395
HpySE526I ACGT 3 cut(s) 62, 1142, 1195
Hsp92II CATG 8 cut(s) 244, 406, 458, 490, 748, 882, 977, 1024
HspAI GCGC 1 cut(s) 990
Kzo9I GATC 3 cut(s) 206, 276, 919
LmnI GCTCC 3 cut(s) 137, 296, 754
LpnPI CCDG 9 cut(s) 237, 337, 604, 888, 914, 1025, 1077, 1092, 1146
LweI GCATC 7 cut(s) 64, 242, 631, 780, 865, 961, 1018
MaeI CTAG 7 cut(s) 101, 117, 521, 624, 914, 1089, 1113
MaeII ACGT 3 cut(s) 62, 1142, 1195
MaeIII GTNAC 4 cut(s) 319, 509, 740, 833
MalI GATC 3 cut(s) 208, 278, 921
MboI GATC 3 cut(s) 206, 276, 919
MboII GAAGA 4 cut(s) 201, 713, 1007, 1016
MflI RGATCY 1 cut(s) 919
MlsI TGGCCA 1 cut(s) 248
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 4 cut(s) 45, 91, 120, 439
MnlI CCTC 5 cut(s) 31, 34, 127, 174, 259
Mox20I TGGCCA 1 cut(s) 248
Mph1103I ATGCAT 2 cut(s) 235, 880
MroXI GAANNNNTTC 1 cut(s) 468
MscI TGGCCA 1 cut(s) 248
MseI TTAA 7 cut(s) 144, 177, 384, 812, 945, 962, 1198
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 248
MspI CCGG 3 cut(s) 324, 1012, 1133
Mva1269I GAATGC 1 cut(s) 450
MvnI CGCG 1 cut(s) 992
MwoI GCNNNNNNNGC 1 cut(s) 889
NdeII GATC 3 cut(s) 206, 276, 919
NlaIII CATG 8 cut(s) 244, 406, 458, 490, 748, 882, 977, 1024
NlaIV GGNNCC 3 cut(s) 139, 750, 921
NmuCI GTSAC 2 cut(s) 319, 509
NsiI ATGCAT 2 cut(s) 235, 880
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 652
PkrI GCNGC 1 cut(s) 721
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PsiI TTATAA 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 319
PspN4I GGNNCC 3 cut(s) 139, 750, 921
PspPI GGNCC 2 cut(s) 1014, 1130
PsuI RGATCY 1 cut(s) 919
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 1 cut(s) 612
SaqAI TTAA 7 cut(s) 144, 177, 384, 812, 945, 962, 1198
SatI GCNGC 1 cut(s) 720
Sau3AI GATC 3 cut(s) 206, 276, 919
Sau96I GGNCC 2 cut(s) 1014, 1130
SchI GAGTC 1 cut(s) 393
SfaNI GCATC 7 cut(s) 64, 242, 631, 780, 865, 961, 1018
SfcI CTRYAG 1 cut(s) 796
SinI GGWCC 1 cut(s) 1130
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 561
SmoI CTYRAG 1 cut(s) 561
SpeI ACTAGT 1 cut(s) 913
SsiI CCGC 2 cut(s) 719, 992
SspI AATATT 1 cut(s) 359
SspMI CTAG 7 cut(s) 101, 117, 521, 624, 914, 1089, 1113
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 5 cut(s) 709, 800, 960, 1097, 1169
TaiI ACGT 3 cut(s) 65, 1145, 1198
TaqI TCGA 1 cut(s) 506
TatI WGTACW 1 cut(s) 483
TauI GCSGC 1 cut(s) 722
TfiI GAWTC 1 cut(s) 652
Tru1I TTAA 7 cut(s) 144, 177, 384, 812, 945, 962, 1198
Tru9I TTAA 7 cut(s) 144, 177, 384, 812, 945, 962, 1198
TseFI GTSAC 2 cut(s) 319, 509
Tsp45I GTSAC 2 cut(s) 319, 509
TspGWI ACGGA 1 cut(s) 769
VpaK11BI GGWCC 1 cut(s) 1130
XapI RAATTY 6 cut(s) 40, 51, 162, 170, 584, 841
XbaI TCTAGA 1 cut(s) 1088
XmiI GTMKAC 1 cut(s) 795
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 7 cut(s) 101, 117, 521, 624, 914, 1089, 1113
Zsp2I ATGCAT 2 cut(s) 235, 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.