Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000922

Physical Location & Seq

Genomic Coordinates Reverse (-)

29668677 .. 29673965

Gene Structure 5289 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1219600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1992 bp

ATGCAAGACATTTTCGGATCAGTTCGCCGATCACTGGTGTTCCGCACAGCATCGGACAATGAAGACTCCCCAGGAGGAACCCTAGTCGACAAGATCAATTCCTGCATTCGGAAATCCAGAGTCTTCTCGAAGCCCTCTCCGCCGCTTCCGTCGCCGGCAATGGCAAAGGAAAACGCACCTCCGATCCGGTGGCGGAAGGGCGAGTTGATCGGCTGCGGTGCCTTTGGGCGTGTTTACATGGGCATGAATCTTGATTCCGGAGAGCTTCTTGCGGTGAAACAGGTTCTGATTGCGGCAAATGGCGCTTCAAAGGAGAAAGCACAGGCTCACATCAAGGAGCTTGAGGAAGAAGTAAAGCTTCTGAAGAATCTGTCTCATCCAAACATTGTAAGATATTTAGGTACGGTGAGGGAGGACGATACCTTGAATATTCTGTTGGAATTTGTCCCTGGTGGATCCATATCATCACTTTTGGGGAAATTTGGATCTTTCCCTGAGGCAGTAATACAAACGTATACCAAGCAGTTGCTATTGGGACTAGAATATTTACACAAAAATGGAATCATGCACAGGGACATTAAGGGAGCAAATATCCTTGTTGATAATAAAGGATGCATCAAACTTGCAGATTTTGGGGCATCCAAACAGGTCGTCGAGCTGGCAACCATTTCTGGTGCTAAGTCTATGAAGGGTACACCATACTGGATGGCTCCTGAAGTTATTCTCCAGACCGGTCATAGCTTCTCTGCTGACATATGGAGTGTTGGATGTACTGTGATTGAGATGGCCACTGGAAAGCCTCCTTGGAGCCAACAGTACCAAGAGGTTGCTGCTCTCTTTCATATAGGGACAACAAAGTCTCATCCACCAATCCCTGAGCATCTATCCTTTGAGGCGAAAGATTTTTTGCTAAAATGTCTGCAAAAGGAACCACATTTGAGGTCAGTTGCATCTGAGTTGCTTCAGCATCCATTCGTAACTGGGCAGAATATGGAATCAGATCCTGTTTTTCGTACTTCTGTCATGGAAGATTCAGAACTCCAATCTCCATCATATTCTCCAAACCTGCACACCTTCGAGATGTCAACTTGCCCAGATTCGGATGATATGTGTAATTTGGGCAGTTTGCAACACTCAACTATACATCCTAACAAGTTGCTGGAAAGTAGAGCCATTTGGGGAAGAAACAGCAATGATGATGATATGTGTCAGATTGATGACAAAGAAGATACTATGGTGGGAGAATTAAAGCTCAGTTCTTCTTTGATGCCTGACAACATCAAGAGCTTTAACCCAATGTGTGAACCCACTGATGATGAGTTGGTGTGCAAGTTTGTTGGAAGTTTAGAAACCAAACAAAATGGAATGGATCTGGGTCTGGATATCAATCACCAAACTGATTTTCCTGCCGGCAACTCTGGAACAATTGGAGAGGGAGAAAAAGATTTTTCATTTCCTTGTGGAACATCACTTGCGGAGGAAGACGATATACTTACTGAATCAAAAATCACAGCTTTTCTGGATGAGAAGGCCCTAGAACTTAAAAAGCTGCAAACACCATTATTTGAAGAGTTCTACAACAGCTTGAATGTAATTTGCTCTCCAAATTTTGTGGATAACTTGCCTGATGAACCCACCCCAAGGTACTTGAAATTGCCTCCCAAAAGTAGATCACCCAGTCGGGGGGTAGCTGATGCTCTTAGAACTGGAAGCCCTGGTAGTAATGGCAGGCGGGTTTCAAATATTGGTAATGCAAATGTTCAAACTGCACACGACATGCCATCACCTCCATGCAGTCAGCAAGAGCCAGGCAGTCCAAGTATGAGCTCCTCTGAGAGAGAGAGGAAGTGGAAAGAAGAGCTTGATCTAGAGCTTGCGAGAAAGCGAGAGATGATGCGCCAAGCAGGTGTAGGAGGGAAAACATTATCTCCAAAGGATCGAGGTTTAAATCGGCAGAGAGAGCGAACTAGGTTTGCATCTCCAGGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0000186 GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0097435 GO:0140096 GO:1901564 GO:1902531 GO:1902533

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

664

Amino Acids

73.1

Weight (kDa)

5.72

Isoelectric Point (pI)

52.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	65 - 326	4.6e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	67 - 322	9.3e-45	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	179 - 254	2.7e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000922)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09000 AT1G09000 AT1G54960 AT1G54960
fragaria_vesca	FvH4_2g08110 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14790 FvH4_4g12990 FvH4_4g34210 FvH4_6g05800 FvH4_6g05800
malus_domestica	MD04G1205500.v1.1 MD12G1219600.v1.1
prunus_persica	Prupe.6G326300_v2.0.a1 Prupe.6G326300_v2.0.a1 Prupe.6G326300_v2.0.a1
pyrus_communis	pycom04g18180 pycom12g20510
rosa_chinensis	RchiOBHm_Chr3g0454721 RchiOBHm_Chr5g0024321 RchiOBHm_Chr5g0024331 RchiOBHm_Chr5g0024401 RchiOBHm_Chr5g0025361
rosa_laevigata	RLG00000032792 RLG00000032796
rosa_multiflora	Rmu_co7974142.1_g000001 Rmu_co8231501.1_g000001 Rmu_co8476593.1_g000001 Rmu_sc0005861.1_g000005 Rmu_sc0014903.1_g000002 Rmu_sc0015218.1_g000003
rosa_roxburghii	Rroxscaffold_1G00054820 Rroxscaffold_1G00054830 Rroxscaffold_1G00055870 Rroxscaffold_6G00424830
rosa_rugosa	Rorug03G0001600 Rorug05G0083100
rosa_samantha	Rh3AG061400 Rh3AG061600 Rh3BG063200 Rh3BG063400 Rh3CG062100 Rh3CG062300 Rh3DG063100 Rh5AG173900 Rh5AG174200 Rh5BG171100 Rh5BG171800 Rh5CG187000 Rh5CG187100 Rh5CG187200 Rh5DG173300 Rh5DG245400
rosa_wichuraiana	Rw3G004800 Rw5G015780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1895
AasI	GACNNNNNNGTC	1 cut(s)	856
Acc36I	ACCTGC	2 cut(s)	1074, 1895
AccB1I	GGYRCC	1 cut(s)	218
AccI	GTMKAC	2 cut(s)	87, 515
AccIII	TCCGGA	1 cut(s)	257
AciI	CCGC	9 cut(s)	43, 140, 143, 193, 216, 272, 293, 1475, 1732
AclWI	GGATC	8 cut(s)	25, 178, 450, 463, 493, 995, 1377, 1944
AcoI	YGGCCR	1 cut(s)	786
AcsI	RAATTY	3 cut(s)	440, 479, 1606
AcuI	CTGAAG	3 cut(s)	383, 735, 947
AfaI	GTAC	6 cut(s)	403, 694, 772, 818, 1015, 1646
AfiI	CCNNNNNNNGG	6 cut(s)	34, 108, 1099, 1641, 1682, 1683
AgeI	ACCGGT	1 cut(s)	731
AgsI	TTSAA	7 cut(s)	309, 427, 1568, 1588, 1651, 1740, 1763
AjnI	CCWGG	5 cut(s)	70, 448, 1714, 1807, 1981
AjuI	GAANNNNNNNTTGG	6 cut(s)	419, 451, 787, 819, 1386, 1418
Alw21I	GWGCWC	1 cut(s)	1829
Alw26I	GTCTC	2 cut(s)	378, 864
AlwI	GGATC	8 cut(s)	25, 178, 450, 463, 493, 995, 1377, 1944
AlwNI	CAGNNNCTG	2 cut(s)	286, 1004
Aor13HI	TCCGGA	1 cut(s)	257
AoxI	GGCC	2 cut(s)	786, 1530
ApeKI	GCWGC	3 cut(s)	213, 830, 1549
ApoI	RAATTY	3 cut(s)	440, 479, 1606
ArsI	GACNNNNNNTTYG	2 cut(s)	636, 668
AsiGI	ACCGGT	1 cut(s)	731
Asp700I	GAANNNNTTC	1 cut(s)	720
AspLEI	GCGC	2 cut(s)	305, 1899
AspS9I	GGNCC	1 cut(s)	1531
AsuHPI	GGTGA	5 cut(s)	286, 418, 1382, 1665, 1776
AxyI	CCTNAGG	1 cut(s)	495
BaeI	ACNNNNGTAYC	2 cut(s)	1221, 1254
BalI	TGGCCA	1 cut(s)	788
BamHI	GGATCC	1 cut(s)	455
BanI	GGYRCC	1 cut(s)	218
BanII	GRGCYC	1 cut(s)	1829
BarI	GAAGNNNNNNTAC	2 cut(s)	1473, 1505
BbsI	GAAGAC	3 cut(s)	69, 115, 1488
Bbv12I	GWGCWC	1 cut(s)	1829
BbvI	GCAGC	3 cut(s)	200, 817, 1536
BccI	CCATC	4 cut(s)	700, 778, 1057, 1789
BciT130I	CCWGG	5 cut(s)	72, 450, 1716, 1809, 1983
BcoDI	GTCTC	2 cut(s)	378, 864
BfaI	CTAG	5 cut(s)	83, 539, 1535, 1868, 1968
BfoI	RGCGCY	1 cut(s)	306
BfuAI	ACCTGC	2 cut(s)	1074, 1895
BisI	GCNGC	5 cut(s)	143, 214, 294, 831, 1550
BlsI	GCNGC	5 cut(s)	144, 215, 295, 832, 1551
Bme1390I	CCNGG	5 cut(s)	72, 450, 1716, 1809, 1983
BmgT120I	GGNCC	1 cut(s)	1531
BmiI	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
BmrFI	CCNGG	5 cut(s)	72, 450, 1716, 1809, 1983
BmrI	ACTGGG	2 cut(s)	990, 1671
BmuI	ACTGGG	2 cut(s)	990, 1671
BpiI	GAAGAC	3 cut(s)	69, 115, 1488
BpmI	CTGGAG	2 cut(s)	710, 1965
Bpu10I	CCTNAGC	1 cut(s)	876
BpuEI	CTTGAG	1 cut(s)	362
BsaBI	GATNNNNATC	2 cut(s)	460, 1386
BsaJI	CCNNGG	5 cut(s)	70, 448, 803, 1640, 1714
BsaWI	WCCGGW	3 cut(s)	186, 257, 731
BsaXI	ACNNNNNCTCC	2 cut(s)	1912, 1942
Bsc4I	CCNNNNNNNGG	6 cut(s)	34, 108, 1099, 1641, 1682, 1683
Bse118I	RCCGGY	3 cut(s)	154, 731, 1409
Bse1I	ACTGG	6 cut(s)	39, 707, 796, 985, 1677, 1711
Bse21I	CCTNAGG	1 cut(s)	495
Bse3DI	GCAATG	2 cut(s)	165, 1198
Bse8I	GATNNNNATC	2 cut(s)	460, 1386
BseAI	TCCGGA	1 cut(s)	257
BseBI	CCWGG	5 cut(s)	72, 450, 1716, 1809, 1983
BseDI	CCNNGG	5 cut(s)	70, 448, 803, 1640, 1714
BseJI	GATNNNNATC	2 cut(s)	460, 1386
BseLI	CCNNNNNNNGG	6 cut(s)	34, 108, 1099, 1641, 1682, 1683
BseMI	GCAATG	2 cut(s)	165, 1198
BseMII	CTCAG	5 cut(s)	486, 867, 945, 1267, 1824
BseNI	ACTGG	6 cut(s)	39, 707, 796, 985, 1677, 1711
BseRI	GAGGAG	1 cut(s)	1819
BseXI	GCAGC	3 cut(s)	200, 817, 1536
BsgI	GTGCAG	2 cut(s)	1052, 1752
BshFI	GGCC	2 cut(s)	788, 1532
BshNI	GGYRCC	1 cut(s)	218
BshTI	ACCGGT	1 cut(s)	731
BsiHKAI	GWGCWC	1 cut(s)	1829
BsiSI	CCGG	5 cut(s)	155, 187, 258, 732, 1410
BslFI	GGGAC	4 cut(s)	431, 549, 587, 862
BslI	CCNNNNNNNGG	6 cut(s)	34, 108, 1099, 1641, 1682, 1683
BsmAI	GTCTC	2 cut(s)	378, 864
BsmFI	GGGAC	4 cut(s)	431, 549, 587, 862
BsmI	GAATGC	1 cut(s)	105
BsnI	GGCC	2 cut(s)	788, 1532
Bsp1286I	GDGCHC	1 cut(s)	1829
Bsp13I	TCCGGA	1 cut(s)	257
BspACI	CCGC	9 cut(s)	43, 140, 143, 193, 216, 272, 293, 1475, 1732
BspANI	GGCC	2 cut(s)	788, 1532
BspCNI	CTCAG	5 cut(s)	487, 868, 946, 1266, 1825
BspEI	TCCGGA	1 cut(s)	257
BspLI	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
BspMI	ACCTGC	2 cut(s)	1074, 1895
BspPI	GGATC	8 cut(s)	25, 178, 450, 463, 493, 995, 1377, 1944
BspQI	GCTCTTC	1 cut(s)	1851
BspT107I	GGYRCC	1 cut(s)	218
BsrDI	GCAATG	2 cut(s)	165, 1198
BsrFI	RCCGGY	3 cut(s)	154, 731, 1409
BsrI	ACTGG	6 cut(s)	39, 707, 796, 985, 1677, 1711
BssAI	RCCGGY	3 cut(s)	154, 731, 1409
BssECI	CCNNGG	5 cut(s)	70, 448, 803, 1640, 1714
BssNAI	GTATAC	1 cut(s)	516
BssT1I	CCWWGG	2 cut(s)	803, 1640
Bst1107I	GTATAC	1 cut(s)	516
Bst2UI	CCWGG	5 cut(s)	72, 450, 1716, 1809, 1983
Bst4CI	ACNGT	3 cut(s)	406, 775, 816
Bst6I	CTCTTC	2 cut(s)	1563, 1851
BstC8I	GCNNGC	5 cut(s)	156, 660, 1411, 1730, 1875
BstDEI	CTNAG	7 cut(s)	495, 678, 876, 954, 1253, 1700, 1833
BstH2I	RGCGCY	1 cut(s)	306
BstHHI	GCGC	2 cut(s)	305, 1899
BstMAI	GTCTC	2 cut(s)	378, 864
BstMWI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1960
BstNI	CCWGG	5 cut(s)	72, 450, 1716, 1809, 1983
BstNSI	RCATGY	1 cut(s)	1780
BstSCI	CCNGG	5 cut(s)	70, 448, 1714, 1807, 1981
BstV1I	GCAGC	3 cut(s)	200, 817, 1536
BstV2I	GAAGAC	3 cut(s)	69, 115, 1488
BstX2I	RGATCY	4 cut(s)	455, 485, 1000, 1369
BstYI	RGATCY	4 cut(s)	455, 485, 1000, 1369
BstZ17I	GTATAC	1 cut(s)	516
Bsu36I	CCTNAGG	1 cut(s)	495
BsuRI	GGCC	2 cut(s)	788, 1532
BtsIMutI	CAGTG	3 cut(s)	32, 789, 1308
BveI	ACCTGC	2 cut(s)	1074, 1895
Cac8I	GCNNGC	5 cut(s)	156, 660, 1411, 1730, 1875
CaiI	CAGNNNCTG	2 cut(s)	286, 1004
CfoI	GCGC	2 cut(s)	305, 1899
Cfr10I	RCCGGY	3 cut(s)	154, 731, 1409
Cfr13I	GGNCC	1 cut(s)	1531
Csp6I	GTAC	6 cut(s)	402, 693, 771, 817, 1014, 1645
CspAI	ACCGGT	1 cut(s)	731
CspCI	CAANNNNNGTGG	2 cut(s)	1593, 1628
CviAII	CATG	6 cut(s)	238, 244, 565, 1024, 1777, 1791
CviQI	GTAC	6 cut(s)	402, 693, 771, 817, 1014, 1645
DdeI	CTNAG	7 cut(s)	495, 678, 876, 954, 1253, 1700, 1833
DraI	TTTAAA	1 cut(s)	1947
DrdI	GACNNNNNNGTC	1 cut(s)	856
DseDI	GACNNNNNNGTC	1 cut(s)	856
EaeI	YGGCCR	1 cut(s)	786
Eam1104I	CTCTTC	2 cut(s)	1563, 1851
EarI	CTCTTC	2 cut(s)	1563, 1851
EciI	GGCGGA	2 cut(s)	129, 208
Ecl136II	GAGCTC	1 cut(s)	1827
Eco130I	CCWWGG	2 cut(s)	803, 1640
Eco24I	GRGCYC	1 cut(s)	1829
Eco32I	GATATC	1 cut(s)	1384
Eco53kI	GAGCTC	1 cut(s)	1827
Eco57I	CTGAAG	3 cut(s)	383, 735, 947
Eco81I	CCTNAGG	1 cut(s)	495
EcoICRI	GAGCTC	1 cut(s)	1827
EcoO109I	RGGNCCY	1 cut(s)	1531
EcoRII	CCWGG	5 cut(s)	70, 448, 1714, 1807, 1981
EcoRV	GATATC	1 cut(s)	1384
EcoT14I	CCWWGG	2 cut(s)	803, 1640
EcoT22I	ATGCAT	1 cut(s)	617
EcoT38I	GRGCYC	1 cut(s)	1829
ErhI	CCWWGG	2 cut(s)	803, 1640
FaeI	CATG	6 cut(s)	241, 247, 568, 1027, 1780, 1794
FalI	AAGNNNNNCTT	4 cut(s)	342, 374, 1845, 1877
FaqI	GGGAC	4 cut(s)	431, 549, 587, 862
FatI	CATG	6 cut(s)	237, 243, 564, 1023, 1776, 1790
FauI	CCCGC	1 cut(s)	1725
FauNDI	CATATG	1 cut(s)	755
FblI	GTMKAC	2 cut(s)	87, 515
Fnu4HI	GCNGC	5 cut(s)	143, 214, 294, 831, 1550
FriOI	GRGCYC	1 cut(s)	1829
Fsp4HI	GCNGC	5 cut(s)	143, 214, 294, 831, 1550
FspBI	CTAG	5 cut(s)	83, 539, 1535, 1868, 1968
GlaI	GCGC	2 cut(s)	304, 1898
GluI	GCNGC	5 cut(s)	143, 214, 294, 831, 1550
GsuI	CTGGAG	2 cut(s)	710, 1965
HaeII	RGCGCY	1 cut(s)	306
HaeIII	GGCC	2 cut(s)	788, 1532
HapII	CCGG	5 cut(s)	155, 187, 258, 732, 1410
HhaI	GCGC	2 cut(s)	305, 1899
Hin1II	CATG	6 cut(s)	241, 247, 568, 1027, 1780, 1794
Hin6I	GCGC	2 cut(s)	303, 1897
HinP1I	GCGC	2 cut(s)	303, 1897
HincII	GTYRAC	2 cut(s)	88, 1086
HindII	GTYRAC	2 cut(s)	88, 1086
HindIII	AAGCTT	1 cut(s)	356
HpaII	CCGG	5 cut(s)	155, 187, 258, 732, 1410
HphI	GGTGA	5 cut(s)	286, 418, 1382, 1665, 1776
Hpy166II	GTNNAC	6 cut(s)	88, 235, 516, 695, 1086, 1304
Hpy8I	GTNNAC	6 cut(s)	88, 235, 516, 695, 1086, 1304
Hpy99I	CGWCG	2 cut(s)	154, 656
HpyAV	CCTTC	4 cut(s)	190, 682, 1084, 1522
HpyCH4III	ACNGT	3 cut(s)	406, 775, 816
HpyCH4IV	ACGT	1 cut(s)	512
HpyF10VI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1960
HpyF3I	CTNAG	7 cut(s)	495, 678, 876, 954, 1253, 1700, 1833
HpySE526I	ACGT	1 cut(s)	512
Hsp92II	CATG	6 cut(s)	241, 247, 568, 1027, 1780, 1794
HspAI	GCGC	2 cut(s)	303, 1897
Kpn2I	TCCGGA	1 cut(s)	257
KroI	GCCGGC	2 cut(s)	154, 1409
KroNI	GCCGGC	2 cut(s)	156, 1411
LguI	GCTCTTC	1 cut(s)	1851
LmnI	GCTCC	5 cut(s)	337, 584, 715, 807, 1832
Lsp1109I	GCAGC	3 cut(s)	200, 817, 1536
MaeI	CTAG	5 cut(s)	83, 539, 1535, 1868, 1968
MaeII	ACGT	1 cut(s)	512
MaeIII	GTNAC	1 cut(s)	976
MfeI	CAATTG	1 cut(s)	1425
MflI	RGATCY	4 cut(s)	455, 485, 1000, 1369
MhlI	GDGCHC	1 cut(s)	1829
MlsI	TGGCCA	1 cut(s)	788
MluCI	AATT	9 cut(s)	97, 440, 479, 1114, 1244, 1425, 1593, 1606, 1652
MluNI	TGGCCA	1 cut(s)	788
MlyI	GAGTC	2 cut(s)	59, 129
MmeI	TCCRAC	3 cut(s)	417, 745, 1318
Mox20I	TGGCCA	1 cut(s)	788
Mph1103I	ATGCAT	1 cut(s)	617
MroI	TCCGGA	1 cut(s)	257
MroNI	GCCGGC	2 cut(s)	154, 1409
MroXI	GAANNNNTTC	1 cut(s)	720
MscI	TGGCCA	1 cut(s)	788
MseI	TTAA	5 cut(s)	579, 1247, 1290, 1542, 1946
MslI	CAYNNNNRTG	3 cut(s)	242, 555, 1789
Msp20I	TGGCCA	1 cut(s)	788
MspI	CCGG	5 cut(s)	155, 187, 258, 732, 1410
MspR9I	CCNGG	5 cut(s)	72, 450, 1716, 1809, 1983
MunI	CAATTG	1 cut(s)	1425
Mva1269I	GAATGC	1 cut(s)	105
MvaI	CCWGG	5 cut(s)	72, 450, 1716, 1809, 1983
MwoI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1960
NaeI	GCCGGC	2 cut(s)	156, 1411
NdeI	CATATG	1 cut(s)	755
NgoMIV	GCCGGC	2 cut(s)	154, 1409
NlaIII	CATG	6 cut(s)	241, 247, 568, 1027, 1780, 1794
NlaIV	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
NsiI	ATGCAT	1 cut(s)	617
NspI	RCATGY	1 cut(s)	1780
PaqCI	CACCTGC	1 cut(s)	1895
PciSI	GCTCTTC	1 cut(s)	1851
PctI	GAATGC	1 cut(s)	105
PdiI	GCCGGC	2 cut(s)	156, 1411
PdmI	GAANNNNTTC	1 cut(s)	720
PfeI	GAWTC	8 cut(s)	247, 254, 367, 561, 995, 1031, 1097, 1499
PinAI	ACCGGT	1 cut(s)	731
PkrI	GCNGC	5 cut(s)	144, 215, 295, 832, 1551
PleI	GAGTC	2 cut(s)	59, 128
PpsI	GAGTC	2 cut(s)	59, 128
Psp124BI	GAGCTC	1 cut(s)	1829
Psp6I	CCWGG	5 cut(s)	70, 448, 1714, 1807, 1981
PspGI	CCWGG	5 cut(s)	70, 448, 1714, 1807, 1981
PspN4I	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
PspPI	GGNCC	1 cut(s)	1531
PstNI	CAGNNNCTG	2 cut(s)	286, 1004
PsuI	RGATCY	4 cut(s)	455, 485, 1000, 1369
RsaI	GTAC	6 cut(s)	403, 694, 772, 818, 1015, 1646
RsaNI	GTAC	6 cut(s)	402, 693, 771, 817, 1014, 1645
RseI	CAYNNNNRTG	3 cut(s)	242, 555, 1789
SacI	GAGCTC	1 cut(s)	1829
SalI	GTCGAC	1 cut(s)	86
SapI	GCTCTTC	1 cut(s)	1851
SaqAI	TTAA	5 cut(s)	579, 1247, 1290, 1542, 1946
SatI	GCNGC	5 cut(s)	143, 214, 294, 831, 1550
Sau96I	GGNCC	1 cut(s)	1531
SchI	GAGTC	2 cut(s)	59, 129
ScrFI	CCNGG	5 cut(s)	72, 450, 1716, 1809, 1983
SduI	GDGCHC	1 cut(s)	1829
SmiMI	CAYNNNNRTG	3 cut(s)	242, 555, 1789
SmlI	CTYRAG	1 cut(s)	341
SmoI	CTYRAG	1 cut(s)	341
Sse9I	AATT	9 cut(s)	97, 440, 479, 1114, 1244, 1425, 1593, 1606, 1652
SsiI	CCGC	9 cut(s)	43, 140, 143, 193, 216, 272, 293, 1475, 1732
SspI	AATATT	3 cut(s)	430, 545, 1744
SspMI	CTAG	5 cut(s)	83, 539, 1535, 1868, 1968
SstI	GAGCTC	1 cut(s)	1829
StyD4I	CCNGG	5 cut(s)	70, 448, 1714, 1807, 1981
StyI	CCWWGG	2 cut(s)	803, 1640
TaaI	ACNGT	3 cut(s)	406, 775, 816
TaiI	ACGT	1 cut(s)	515
TaqI	TCGA	5 cut(s)	87, 128, 654, 1077, 1939
TasI	AATT	9 cut(s)	97, 440, 479, 1114, 1244, 1425, 1593, 1606, 1652
TatI	WGTACW	1 cut(s)	770
TauI	GCSGC	2 cut(s)	145, 296
TfiI	GAWTC	8 cut(s)	247, 254, 367, 561, 995, 1031, 1097, 1499
Tru1I	TTAA	5 cut(s)	579, 1247, 1290, 1542, 1946
Tru9I	TTAA	5 cut(s)	579, 1247, 1290, 1542, 1946
TscAI	CASTG	3 cut(s)	39, 796, 1315
TseI	GCWGC	3 cut(s)	213, 830, 1549
TspDTI	ATGAA	6 cut(s)	75, 260, 701, 830, 1440, 1644
TspGWI	ACGGA	1 cut(s)	138
TspRI	CASTG	3 cut(s)	39, 796, 1315
XapI	RAATTY	3 cut(s)	440, 479, 1606
XbaI	TCTAGA	1 cut(s)	1867
XceI	RCATGY	1 cut(s)	1780
XmiI	GTMKAC	2 cut(s)	87, 515
XmnI	GAANNNNTTC	1 cut(s)	720
XspI	CTAG	5 cut(s)	83, 539, 1535, 1868, 1968
Zsp2I	ATGCAT	1 cut(s)	617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.