Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000922

Physical Location & Seq

Genomic Coordinates Reverse (-)

29163327 .. 29168739

Gene Structure 5413 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1205500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGCAAGATATATTCGGATCAGTCCGCCGATCACTGGTGTTCCGCACAGCATCGGAGAACGAGGACTCCCCAGGAGGAACCCTAGTCGACAGGATCAATTCCTGCATTCGGAAATCCAGAGTCTTCTCGAAGCCCTCGCCGCCGCTTCCATTGCCGGCAATGGCGAAGGAAAACGCACCTCCGATCCGGTGGCGGAAGGGCGAGTTGATCGGCTGCGGTGCCTTTGGGCGTGTGTATATGGGAATGAATCTTGATTCCGGAGAGCTTCTTGCTGTTAAACAGGTTCTGATTGCTGCAAATAGTGCTTCAAAGGAGAAAGCACAGGCTCACATCAAGGAGCTTGAGGAAGAAGTGAAGCTTCTAAAGAATCTGTCTCATCCGAACATCGTTAGATATTTGGGTACAGTGAGGGAAGAGGACACCTTAAATATTCTGTTGGAATTTGTCCCTGGTGGATCCATATCATCGCTTTTGGGTAAATTTGGATCTTTTCCTGAGGCTGTTATAAGAACCTACACCAACCAGTTGTTATTGGGGCTAGAATATTTACACAAAAATGGAATCATGCACAGGGACATTAAGGGAGCAAATATCCTTGTTGATAATAAGGGATGCATCAAACTTGCAGATTTTGGGGCATCCAAACAGGTCGTAGAGCTGGCAACCATTTCTGGGGCTAAGTCTATGAAGGGTACACCATACTGGATGGCTCCTGAAGTTATTCTCCAGACTGGTCATAGCTTCTCGGCTGATATATGGAGTGTTGGATGTACTGTGATTGAGATGGCCACTGGAAAGCCTCCTTGGAGCCAACAGTACCAAGAGGTTGCTGCTCTCTTCCATATAGGGACAACAAAGTCTCATCCACCAATCCCTGAGCATCTTTCCTTTGAGGCAAAAGATTTTTTGCTAAAATGTCTGCAAAAGGAACCACATTTGAGGTCGGTGGCATCTGAGTTGCTTCAGCATCCATTCGTAACTGGGGAACATATGGAATCTCATCCTGTTTTTCGTACTTCTGTCATGGAAGATTCAGAACTCCGATCTCCATCATATTCTCCCAACCTGCACACCTTTGAGATGTCAACTTGCCCCGATTCAGATGATATGTGTAATTTGGGCAGTTTGCAACACTCAACTACACATCCTAACGAGTTCCTGGAAAGCAGACCCATTTGGGGAAGAAACAGCTGTGATGATGACATGTGTCAGATTGATGACAAAGACGATACTATGGTGGGCGAAGTAAAGCTCGGTTCTTCTTTGATGCTTGGCAACATCAAGAGTTTTAACCCAATGTGCGAACCCACTGATGATGAGTTGGTGTGCAAGTTTGGTGGAAATTTAGAAAAAGAACAAAACGGAACGGATCTGGATACCACTCACCAAATTGATTTTCCTGCCGGAAACTGCGGAACAATTGGAGACGGAGATAAAGGTTTTTCATTTCCTTGCGGTGCATCACTTGTGGAGGAAGATGATATACTTACTGAATCAAAAATCACGGCATTTTTGGATGAGAAGGCCCTAGAACTTAAAAAGCTGCAAACGCCATTATTTGAAGAGTTCTACAACAGCTTGAATGTAATTTGCTCTCCAAATTTTGCGGATAACTCGCCTGATGAACGCACCTCAAGGTACTTGAAATTACCTCCCAAAAGTAGATCACCTAGTCGGGGAGTAGCTGATGCTCTTATTACTGGAAACCCTGGGAGCAATGCCAGGCGCGTTTCAAATATTGGCAATGCAAATGTTCAAATTGCACAGGACATGCCATCACCTCCACGCAGTCAGCAAGAGCCAGGCAGTCCAAGTATAAGCTCCTCTGAAAGAGAGAGGAAGTGGAAAGAAGAGCTTGATCTTGAGCTTGCAAGAAAGCGAGAGATGATGCGCCAAGCAGGTGTAGGAGGGAAGACATCATCTCCGAAGGATCGAGGTTTAAATAGGCAGAGAGAACGTACTAGGTTTGCTTCTCCAGGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000165 GO:0000186 GO:0000226 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0006996 GO:0007010 GO:0007017 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030865 GO:0031098 GO:0031122 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043622 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0071900 GO:0071902 GO:0080090 GO:0097435 GO:0140096 GO:1901564 GO:1902531 GO:1902533

KEGG Pathways

Metabolic & Signaling

ko04016 map04016

Pfam Domains

Protein Families

Pkinase

Protein Analysis

662

Amino Acids

72.97

Weight (kDa)

5.83

Isoelectric Point (pI)

51.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	65 - 326	2.5e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	67 - 322	6.6e-45	Protein tyrosine and serine/threonine kinase
Pkinase_fungal	PF17667	179 - 254	2.9e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000922)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09000 AT1G09000 AT1G54960 AT1G54960
fragaria_vesca	FvH4_2g08110 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14780 FvH4_3g14790 FvH4_4g12990 FvH4_4g34210 FvH4_6g05800 FvH4_6g05800
malus_domestica	MD04G1205500.v1.1 MD12G1219600.v1.1
prunus_persica	Prupe.6G326300_v2.0.a1 Prupe.6G326300_v2.0.a1 Prupe.6G326300_v2.0.a1
pyrus_communis	pycom04g18180 pycom12g20510
rosa_chinensis	RchiOBHm_Chr3g0454721 RchiOBHm_Chr5g0024321 RchiOBHm_Chr5g0024331 RchiOBHm_Chr5g0024401 RchiOBHm_Chr5g0025361
rosa_laevigata	RLG00000032792 RLG00000032796
rosa_multiflora	Rmu_co7974142.1_g000001 Rmu_co8231501.1_g000001 Rmu_co8476593.1_g000001 Rmu_sc0005861.1_g000005 Rmu_sc0014903.1_g000002 Rmu_sc0015218.1_g000003
rosa_roxburghii	Rroxscaffold_1G00054820 Rroxscaffold_1G00054830 Rroxscaffold_1G00055870 Rroxscaffold_6G00424830
rosa_rugosa	Rorug03G0001600 Rorug05G0083100
rosa_samantha	Rh3AG061400 Rh3AG061600 Rh3BG063200 Rh3BG063400 Rh3CG062100 Rh3CG062300 Rh3DG063100 Rh5AG173900 Rh5AG174200 Rh5BG171100 Rh5BG171800 Rh5CG187000 Rh5CG187100 Rh5CG187200 Rh5DG173300 Rh5DG245400
rosa_wichuraiana	Rw3G004800 Rw5G015780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	506
AarI	CACCTGC	1 cut(s)	1889
AasI	GACNNNNNNGTC	1 cut(s)	856
Acc36I	ACCTGC	2 cut(s)	1074, 1889
AccB1I	GGYRCC	1 cut(s)	218
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	1 cut(s)	1728
AccIII	TCCGGA	1 cut(s)	257
AciI	CCGC	9 cut(s)	25, 43, 140, 143, 193, 216, 1413, 1455, 1607
AclWI	GGATC	8 cut(s)	25, 101, 178, 450, 463, 493, 1377, 1938
AcoI	YGGCCR	1 cut(s)	786
AcsI	RAATTY	4 cut(s)	440, 479, 1342, 1600
AcuI	CTGAAG	2 cut(s)	735, 947
AfaI	GTAC	7 cut(s)	403, 694, 772, 818, 1015, 1640, 1960
AfiI	CCNNNNNNNGG	5 cut(s)	34, 108, 672, 1178, 1676
AflIII	ACRYGT	1 cut(s)	1203
AgsI	TTSAA	6 cut(s)	309, 1562, 1582, 1645, 1734, 1757
AjnI	CCWGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
AjuI	GAANNNNNNNTTGG	4 cut(s)	787, 819, 1380, 1412
AloI	GAACNNNNNNTCC	2 cut(s)	50, 82
Alw26I	GTCTC	3 cut(s)	378, 864, 1419
AlwI	GGATC	8 cut(s)	25, 101, 178, 450, 463, 493, 1377, 1938
AlwNI	CAGNNNCTG	1 cut(s)	286
Aor13HI	TCCGGA	1 cut(s)	257
AoxI	GGCC	2 cut(s)	786, 1524
ApeKI	GCWGC	4 cut(s)	213, 293, 830, 1543
ApoI	RAATTY	4 cut(s)	440, 479, 1342, 1600
Asp700I	GAANNNNTTC	1 cut(s)	720
AspLEI	GCGC	2 cut(s)	1728, 1893
AspS9I	GGNCC	1 cut(s)	1525
AsuHPI	GGTGA	3 cut(s)	1376, 1659, 1770
AxyI	CCTNAGG	1 cut(s)	495
BaeI	ACNNNNGTAYC	2 cut(s)	1221, 1254
BalI	TGGCCA	1 cut(s)	788
BamHI	GGATCC	1 cut(s)	455
BanI	GGYRCC	1 cut(s)	218
BarI	GAAGNNNNNNTAC	2 cut(s)	1467, 1499
BbsI	GAAGAC	2 cut(s)	115, 1919
BbvI	GCAGC	4 cut(s)	200, 280, 817, 1530
BccI	CCATC	4 cut(s)	700, 778, 1057, 1783
BceAI	ACGGC	1 cut(s)	1521
BciT130I	CCWGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
BciVI	GTATCC	1 cut(s)	1369
BcoDI	GTCTC	3 cut(s)	378, 864, 1419
BfaI	CTAG	5 cut(s)	83, 539, 1529, 1671, 1962
BfuAI	ACCTGC	2 cut(s)	1074, 1889
BfuI	GTATCC	1 cut(s)	1369
BisI	GCNGC	6 cut(s)	140, 143, 214, 294, 831, 1544
BlsI	GCNGC	6 cut(s)	141, 144, 215, 295, 832, 1545
Bme1390I	CCNGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
BmgT120I	GGNCC	1 cut(s)	1525
BmiI	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
BmrFI	CCNGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
BmrI	ACTGGG	1 cut(s)	990
BmuI	ACTGGG	1 cut(s)	990
BoxI	GACNNNNGTC	1 cut(s)	1206
BpiI	GAAGAC	2 cut(s)	115, 1919
BpmI	CTGGAG	2 cut(s)	710, 1959
Bpu10I	CCTNAGC	1 cut(s)	876
BpuEI	CTTGAG	3 cut(s)	362, 1618, 1883
BsaBI	GATNNNNATC	2 cut(s)	460, 1048
BsaJI	CCNNGG	5 cut(s)	70, 448, 803, 1708, 1709
BsaWI	WCCGGW	2 cut(s)	186, 257
BsaXI	ACNNNNNCTCC	4 cut(s)	50, 80, 1906, 1936
Bsc4I	CCNNNNNNNGG	5 cut(s)	34, 108, 672, 1178, 1676
Bse118I	RCCGGY	1 cut(s)	154
Bse1I	ACTGG	7 cut(s)	39, 523, 707, 736, 796, 985, 1705
Bse21I	CCTNAGG	1 cut(s)	495
Bse3DI	GCAATG	4 cut(s)	149, 165, 1723, 1750
Bse8I	GATNNNNATC	2 cut(s)	460, 1048
BseAI	TCCGGA	1 cut(s)	257
BseBI	CCWGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
BseDI	CCNNGG	5 cut(s)	70, 448, 803, 1708, 1709
BseJI	GATNNNNATC	2 cut(s)	460, 1048
BseLI	CCNNNNNNNGG	5 cut(s)	34, 108, 672, 1178, 1676
BseMI	GCAATG	4 cut(s)	149, 165, 1723, 1750
BseMII	CTCAG	3 cut(s)	486, 867, 945
BseNI	ACTGG	7 cut(s)	39, 523, 707, 736, 796, 985, 1705
BseRI	GAGGAG	1 cut(s)	1813
BseXI	GCAGC	4 cut(s)	200, 280, 817, 1530
BsgI	GTGCAG	1 cut(s)	1052
Bsh1236I	CGCG	1 cut(s)	1728
BshFI	GGCC	2 cut(s)	788, 1526
BshNI	GGYRCC	1 cut(s)	218
BsiSI	CCGG	4 cut(s)	155, 187, 258, 1404
BslFI	GGGAC	3 cut(s)	431, 587, 862
BslI	CCNNNNNNNGG	5 cut(s)	34, 108, 672, 1178, 1676
BsmAI	GTCTC	3 cut(s)	378, 864, 1419
BsmBI	CGTCTC	1 cut(s)	1419
BsmFI	GGGAC	3 cut(s)	431, 587, 862
BsmI	GAATGC	1 cut(s)	105
BsnI	GGCC	2 cut(s)	788, 1526
Bsp13I	TCCGGA	1 cut(s)	257
BspACI	CCGC	9 cut(s)	25, 43, 140, 143, 193, 216, 1413, 1455, 1607
BspANI	GGCC	2 cut(s)	788, 1526
BspCNI	CTCAG	3 cut(s)	487, 868, 946
BspEI	TCCGGA	1 cut(s)	257
BspFNI	CGCG	1 cut(s)	1728
BspLI	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
BspMI	ACCTGC	2 cut(s)	1074, 1889
BspPI	GGATC	8 cut(s)	25, 101, 178, 450, 463, 493, 1377, 1938
BspQI	GCTCTTC	1 cut(s)	1845
BspT107I	GGYRCC	1 cut(s)	218
BsrDI	GCAATG	4 cut(s)	149, 165, 1723, 1750
BsrFI	RCCGGY	1 cut(s)	154
BsrI	ACTGG	7 cut(s)	39, 523, 707, 736, 796, 985, 1705
BssAI	RCCGGY	1 cut(s)	154
BssECI	CCNNGG	5 cut(s)	70, 448, 803, 1708, 1709
BssT1I	CCWWGG	1 cut(s)	803
Bst2UI	CCWGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
Bst4CI	ACNGT	3 cut(s)	406, 775, 816
Bst6I	CTCTTC	4 cut(s)	408, 842, 1557, 1845
BstAPI	GCANNNNNTGC	1 cut(s)	302
BstC8I	GCNNGC	3 cut(s)	156, 660, 1869
BstDEI	CTNAG	4 cut(s)	495, 678, 876, 954
BstFNI	CGCG	1 cut(s)	1728
BstHHI	GCGC	2 cut(s)	1728, 1893
BstMAI	GTCTC	3 cut(s)	378, 864, 1419
BstMWI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1549
BstNI	CCWGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
BstNSI	RCATGY	2 cut(s)	1207, 1774
BstPAI	GACNNNNGTC	1 cut(s)	1206
BstSCI	CCNGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
BstUI	CGCG	1 cut(s)	1728
BstV1I	GCAGC	4 cut(s)	200, 280, 817, 1530
BstV2I	GAAGAC	2 cut(s)	115, 1919
BstX2I	RGATCY	3 cut(s)	455, 485, 1369
BstYI	RGATCY	3 cut(s)	455, 485, 1369
Bsu36I	CCTNAGG	1 cut(s)	495
BsuI	GTATCC	1 cut(s)	1369
BsuRI	GGCC	2 cut(s)	788, 1526
BtgZI	GCGATG	1 cut(s)	450
BtsIMutI	CAGTG	4 cut(s)	32, 411, 789, 1308
BveI	ACCTGC	2 cut(s)	1074, 1889
Cac8I	GCNNGC	3 cut(s)	156, 660, 1869
CaiI	CAGNNNCTG	1 cut(s)	286
CfoI	GCGC	2 cut(s)	1728, 1893
Cfr10I	RCCGGY	1 cut(s)	154
Cfr13I	GGNCC	1 cut(s)	1525
Csp6I	GTAC	7 cut(s)	402, 693, 771, 817, 1014, 1639, 1959
CspCI	CAANNNNNGTGG	2 cut(s)	1318, 1353
CviAII	CATG	4 cut(s)	565, 1024, 1204, 1771
CviQI	GTAC	7 cut(s)	402, 693, 771, 817, 1014, 1639, 1959
DdeI	CTNAG	4 cut(s)	495, 678, 876, 954
DraI	TTTAAA	1 cut(s)	1941
DrdI	GACNNNNNNGTC	1 cut(s)	856
DseDI	GACNNNNNNGTC	1 cut(s)	856
EaeI	YGGCCR	1 cut(s)	786
Eam1104I	CTCTTC	4 cut(s)	408, 842, 1557, 1845
EarI	CTCTTC	4 cut(s)	408, 842, 1557, 1845
EciI	GGCGGA	2 cut(s)	14, 208
Eco130I	CCWWGG	1 cut(s)	803
Eco57I	CTGAAG	2 cut(s)	735, 947
Eco81I	CCTNAGG	1 cut(s)	495
EcoO109I	RGGNCCY	1 cut(s)	1525
EcoRII	CCWGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
EcoT14I	CCWWGG	1 cut(s)	803
EcoT22I	ATGCAT	1 cut(s)	617
ErhI	CCWWGG	1 cut(s)	803
Esp3I	CGTCTC	1 cut(s)	1419
FaeI	CATG	4 cut(s)	568, 1027, 1207, 1774
FalI	AAGNNNNNCTT	4 cut(s)	342, 374, 1839, 1871
FaqI	GGGAC	3 cut(s)	431, 587, 862
FatI	CATG	4 cut(s)	564, 1023, 1203, 1770
FauNDI	CATATG	1 cut(s)	990
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	6 cut(s)	140, 143, 214, 294, 831, 1544
Fsp4HI	GCNGC	6 cut(s)	140, 143, 214, 294, 831, 1544
FspBI	CTAG	5 cut(s)	83, 539, 1529, 1671, 1962
GlaI	GCGC	2 cut(s)	1727, 1892
GluI	GCNGC	6 cut(s)	140, 143, 214, 294, 831, 1544
GsuI	CTGGAG	2 cut(s)	710, 1959
HaeIII	GGCC	2 cut(s)	788, 1526
HapII	CCGG	4 cut(s)	155, 187, 258, 1404
HhaI	GCGC	2 cut(s)	1728, 1893
Hin1II	CATG	4 cut(s)	568, 1027, 1207, 1774
Hin6I	GCGC	2 cut(s)	1726, 1891
HinP1I	GCGC	2 cut(s)	1726, 1891
HincII	GTYRAC	2 cut(s)	88, 1086
HindII	GTYRAC	2 cut(s)	88, 1086
HindIII	AAGCTT	1 cut(s)	356
HpaII	CCGG	4 cut(s)	155, 187, 258, 1404
HphI	GGTGA	3 cut(s)	1376, 1659, 1770
Hpy166II	GTNNAC	3 cut(s)	88, 695, 1086
Hpy8I	GTNNAC	3 cut(s)	88, 695, 1086
HpyAV	CCTTC	5 cut(s)	160, 190, 682, 1516, 1921
HpyCH4III	ACNGT	3 cut(s)	406, 775, 816
HpyCH4IV	ACGT	1 cut(s)	1957
HpyF10VI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1549
HpyF3I	CTNAG	4 cut(s)	495, 678, 876, 954
HpySE526I	ACGT	1 cut(s)	1957
Hsp92II	CATG	4 cut(s)	568, 1027, 1207, 1774
HspAI	GCGC	2 cut(s)	1726, 1891
Kpn2I	TCCGGA	1 cut(s)	257
KroI	GCCGGC	1 cut(s)	154
KroNI	GCCGGC	1 cut(s)	156
LguI	GCTCTTC	1 cut(s)	1845
LmnI	GCTCC	6 cut(s)	337, 584, 715, 807, 1713, 1826
Lsp1109I	GCAGC	4 cut(s)	200, 280, 817, 1530
MaeI	CTAG	5 cut(s)	83, 539, 1529, 1671, 1962
MaeII	ACGT	1 cut(s)	1957
MaeIII	GTNAC	1 cut(s)	976
MfeI	CAATTG	1 cut(s)	1419
MflI	RGATCY	3 cut(s)	455, 485, 1369
MlsI	TGGCCA	1 cut(s)	788
MluNI	TGGCCA	1 cut(s)	788
MlyI	GAGTC	2 cut(s)	59, 129
MmeI	TCCRAC	2 cut(s)	417, 745
Mox20I	TGGCCA	1 cut(s)	788
Mph1103I	ATGCAT	1 cut(s)	617
MroI	TCCGGA	1 cut(s)	257
MroNI	GCCGGC	1 cut(s)	154
MroXI	GAANNNNTTC	1 cut(s)	720
MscI	TGGCCA	1 cut(s)	788
MseI	TTAA	6 cut(s)	276, 425, 579, 1290, 1536, 1940
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	788
MspA1I	CMGCKG	1 cut(s)	1191
MspI	CCGG	4 cut(s)	155, 187, 258, 1404
MspR9I	CCNGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
MunI	CAATTG	1 cut(s)	1419
Mva1269I	GAATGC	1 cut(s)	105
MvaI	CCWGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
MvnI	CGCG	1 cut(s)	1728
MwoI	GCNNNNNNNGC	4 cut(s)	139, 151, 302, 1549
NaeI	GCCGGC	1 cut(s)	156
NdeI	CATATG	1 cut(s)	990
NgoMIV	GCCGGC	1 cut(s)	154
NlaIII	CATG	4 cut(s)	568, 1027, 1207, 1774
NlaIV	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
NmeAIII	GCCGAG	1 cut(s)	725
NsiI	ATGCAT	1 cut(s)	617
NspI	RCATGY	2 cut(s)	1207, 1774
PaqCI	CACCTGC	1 cut(s)	1889
PasI	CCCWGGG	1 cut(s)	1709
PciI	ACATGT	1 cut(s)	1203
PciSI	GCTCTTC	1 cut(s)	1845
PctI	GAATGC	1 cut(s)	105
PdiI	GCCGGC	1 cut(s)	156
PdmI	GAANNNNTTC	1 cut(s)	720
PfeI	GAWTC	8 cut(s)	247, 254, 367, 561, 995, 1031, 1097, 1493
PfoI	TCCNGGA	1 cut(s)	1158
PkrI	GCNGC	6 cut(s)	141, 144, 215, 295, 832, 1545
PleI	GAGTC	2 cut(s)	59, 128
PpsI	GAGTC	2 cut(s)	59, 128
PscI	ACATGT	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	1206
PsiI	TTATAA	1 cut(s)	506
Psp6I	CCWGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
PspGI	CCWGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
PspN4I	GGNNCC	6 cut(s)	79, 220, 457, 711, 809, 930
PspPI	GGNCC	1 cut(s)	1525
PstNI	CAGNNNCTG	1 cut(s)	286
PsuI	RGATCY	3 cut(s)	455, 485, 1369
PvuII	CAGCTG	1 cut(s)	1191
RsaI	GTAC	7 cut(s)	403, 694, 772, 818, 1015, 1640, 1960
RsaNI	GTAC	7 cut(s)	402, 693, 771, 817, 1014, 1639, 1959
RseI	CAYNNNNRTG	1 cut(s)	555
SalI	GTCGAC	1 cut(s)	86
SapI	GCTCTTC	1 cut(s)	1845
SaqAI	TTAA	6 cut(s)	276, 425, 579, 1290, 1536, 1940
SatI	GCNGC	6 cut(s)	140, 143, 214, 294, 831, 1544
Sau96I	GGNCC	1 cut(s)	1525
SchI	GAGTC	2 cut(s)	59, 129
ScrFI	CCNGG	7 cut(s)	72, 450, 1160, 1710, 1723, 1803, 1977
SmiMI	CAYNNNNRTG	1 cut(s)	555
SmlI	CTYRAG	3 cut(s)	341, 1633, 1862
SmoI	CTYRAG	3 cut(s)	341, 1633, 1862
SsiI	CCGC	9 cut(s)	25, 43, 140, 143, 193, 216, 1413, 1455, 1607
SspI	AATATT	3 cut(s)	430, 545, 1738
SspMI	CTAG	5 cut(s)	83, 539, 1529, 1671, 1962
StyD4I	CCNGG	7 cut(s)	70, 448, 1158, 1708, 1721, 1801, 1975
StyI	CCWWGG	1 cut(s)	803
TaaI	ACNGT	3 cut(s)	406, 775, 816
TaiI	ACGT	1 cut(s)	1960
TaqI	TCGA	3 cut(s)	87, 128, 1933
TatI	WGTACW	1 cut(s)	770
TauI	GCSGC	2 cut(s)	142, 145
TfiI	GAWTC	8 cut(s)	247, 254, 367, 561, 995, 1031, 1097, 1493
Tru1I	TTAA	6 cut(s)	276, 425, 579, 1290, 1536, 1940
Tru9I	TTAA	6 cut(s)	276, 425, 579, 1290, 1536, 1940
TscAI	CASTG	4 cut(s)	39, 411, 796, 1315
TseI	GCWGC	4 cut(s)	213, 293, 830, 1543
TspDTI	ATGAA	4 cut(s)	260, 701, 1434, 1638
TspGWI	ACGGA	3 cut(s)	1377, 1382, 1443
TspRI	CASTG	4 cut(s)	39, 411, 796, 1315
XapI	RAATTY	4 cut(s)	440, 479, 1342, 1600
XceI	RCATGY	2 cut(s)	1207, 1774
XmiI	GTMKAC	1 cut(s)	87
XmnI	GAANNNNTTC	1 cut(s)	720
XspI	CTAG	5 cut(s)	83, 539, 1529, 1671, 1962
Zsp2I	ATGCAT	1 cut(s)	617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.