Rh5AG174200

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000922
Physical Location & Seq
Reverse (-)
19246433 .. 19251553
5121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG174200.1

Sequence Viewer

Length: 822 bp
ATGTCTTCAATCCCCAGAGACGAAGCAGCCTCGATTCAATGGCAGAAGGGCGATTTGATCGGTTCCGGGGCCTTTGGCCGTGTTTACATGGGCATGAATACCGAATCCGGAGAGCTTATTGCCGCAAAAGAGGCTCATACCAAGGTACTTCAAGATGAAGTGAGGCTTTTAGAAAACCTGTCGCATCCGAAAATTATTAGATGTTTAGGAACCGAGAGGGAGGATGAAACCTTAACTATGCTGTTGGAATATGTACCTGGAGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTCTCAGTTCCTGTAATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTAGAATATTTACACAAAAAAGGAATCATACATGGGGACATTAAGGGAGCAAATATCCTTGTTGATAATAAAGGGCGCATTAAACTAGCAGATTTTGGTGCATCCAAACAGGCAGCCGTTTCAGGTGCAAAGCCTACAAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTTGTGGGACTGGTCATGACTTCTCTGCAGATATATGGAGCGTTGGATGCACTGTGATTGAGATGGCCACTGGAAATCCTCCTTGGAACCAACAGTTTGAAGCGGGACAAGCAAGTCTCATCCACCAATCCCTGATCATCTTTCTCCTGCAGCAAAAGATTTTCTGCACGAATGTCTGCAAAAAGATGCATGAAAGGATAAAAGAGCCTGCCCCAGATCATTCATTAACATGGCAATACACCGGCCCATACATTTCTGTTACCATCCAAAGCACCCAAGTCGAACTGGAAGTACTGGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

273

Amino Acids

30.11

Weight (kDa)

6.45

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 224 6.1e-58 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 17 - 204 5.7e-35 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 48 - 152 8.1e-08 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 630
AccIII TCCGGA 1 cut(s) 107
AciI CCGC 2 cut(s) 123, 620
AclWI GGATC 3 cut(s) 258, 271, 301
AcoI YGGCCR 2 cut(s) 76, 582
AcsI RAATTY 1 cut(s) 287
AcuI CTGAAG 1 cut(s) 531
AfaI GTAC 4 cut(s) 147, 255, 490, 810
AfiI CCNNNNNNNGG 1 cut(s) 485
AgsI TTSAA 4 cut(s) 9, 38, 152, 617
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 2 cut(s) 583, 615
AluBI AGCT 2 cut(s) 115, 818
AluI AGCT 2 cut(s) 115, 818
Alw26I GTCTC 2 cut(s) 12, 638
AlwI GGATC 3 cut(s) 258, 271, 301
AlwNI CAGNNNCTG 1 cut(s) 308
Aor13HI TCCGGA 1 cut(s) 107
AoxI GGCC 4 cut(s) 69, 76, 582, 760
ApeKI GCWGC 3 cut(s) 26, 458, 667
ApoI RAATTY 1 cut(s) 287
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 2 cut(s) 69, 761
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 316
BalI TGGCCA 1 cut(s) 584
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 38, 470, 679
BccI CCATC 3 cut(s) 496, 574, 788
BceAI ACGGC 2 cut(s) 63, 446
BcgI CGANNNNNNTGC 2 cut(s) 778, 812
BciT130I CCWGG 1 cut(s) 258
BclI TGATCA 1 cut(s) 651
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 2 cut(s) 12, 638
BfaI CTAG 2 cut(s) 347, 431
BfmI CTRYAG 2 cut(s) 543, 665
BisI GCNGC 4 cut(s) 27, 123, 459, 668
BlsI GCNGC 4 cut(s) 28, 124, 460, 669
BmcAI AGTACT 1 cut(s) 810
Bme1390I CCNGG 2 cut(s) 67, 258
BmgT120I GGNCC 2 cut(s) 69, 761
BmiI GGNNCC 6 cut(s) 64, 70, 211, 265, 507, 605
BmrFI CCNGG 2 cut(s) 67, 258
BmsI GCATC 4 cut(s) 193, 455, 554, 693
BpmI CTGGAG 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 66, 141, 599
BsaWI WCCGGW 1 cut(s) 107
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 5 cut(s) 503, 532, 592, 807, 816
BseAI TCCGGA 1 cut(s) 107
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 66, 141, 599
BseGI GGATG 7 cut(s) 184, 229, 446, 507, 569, 636, 780
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 5 cut(s) 503, 532, 592, 807, 816
BseXI GCAGC 3 cut(s) 38, 470, 679
BsgI GTGCAG 1 cut(s) 667
BshFI GGCC 4 cut(s) 71, 78, 584, 762
BsiSI CCGG 3 cut(s) 66, 108, 759
BslFI GGGAC 3 cut(s) 395, 538, 636
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 2 cut(s) 12, 638
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 395, 538, 636
BsnI GGCC 4 cut(s) 71, 78, 584, 762
Bsp13I TCCGGA 1 cut(s) 107
Bsp143I GATC 5 cut(s) 57, 263, 293, 651, 733
BspACI CCGC 2 cut(s) 123, 620
BspANI GGCC 4 cut(s) 71, 78, 584, 762
BspCNI CTCAG 1 cut(s) 314
BspEI TCCGGA 1 cut(s) 107
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 6 cut(s) 64, 70, 211, 265, 507, 605
BspMAI CTGCAG 2 cut(s) 547, 669
BspPI GGATC 3 cut(s) 258, 271, 301
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 5 cut(s) 503, 532, 592, 807, 816
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 3 cut(s) 66, 141, 599
BssMI GATC 5 cut(s) 57, 263, 293, 651, 733
BssT1I CCWWGG 2 cut(s) 141, 599
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 2 cut(s) 571, 612
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 342, 726
BstDEI CTNAG 1 cut(s) 301
BstF5I GGATG 7 cut(s) 184, 229, 446, 507, 569, 636, 780
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 5 cut(s) 60, 266, 296, 654, 736
BstMAI GTCTC 2 cut(s) 12, 638
BstMBI GATC 5 cut(s) 57, 263, 293, 651, 733
BstMWI GCNNNNNNNGC 4 cut(s) 131, 337, 564, 626
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 65, 256
BstSFI CTRYAG 2 cut(s) 543, 665
BstV1I GCAGC 3 cut(s) 38, 470, 679
BstX2I RGATCY 2 cut(s) 263, 293
BstYI RGATCY 2 cut(s) 263, 293
BsuRI GGCC 4 cut(s) 71, 78, 584, 762
BtsCI GGATG 7 cut(s) 184, 229, 446, 507, 569, 636, 780
BtsIMutI CAGTG 2 cut(s) 567, 585
Cac8I GCNNGC 2 cut(s) 342, 726
CaiI CAGNNNCTG 1 cut(s) 308
CciI TCATGA 1 cut(s) 532
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 2 cut(s) 69, 761
Csp6I GTAC 4 cut(s) 146, 254, 489, 809
CviAII CATG 6 cut(s) 88, 94, 377, 533, 707, 747
CviQI GTAC 4 cut(s) 146, 254, 489, 809
DdeI CTNAG 1 cut(s) 301
DpnI GATC 5 cut(s) 59, 265, 295, 653, 735
DpnII GATC 5 cut(s) 57, 263, 293, 651, 733
DrdI GACNNNNNNGTC 1 cut(s) 630
DseDI GACNNNNNNGTC 1 cut(s) 630
EaeI YGGCCR 2 cut(s) 76, 582
Eco130I CCWWGG 2 cut(s) 141, 599
Eco57I CTGAAG 1 cut(s) 531
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 141, 599
EcoT22I ATGCAT 1 cut(s) 708
ErhI CCWWGG 2 cut(s) 141, 599
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 6 cut(s) 91, 97, 380, 536, 710, 750
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 3 cut(s) 395, 538, 636
FatI CATG 6 cut(s) 87, 93, 376, 532, 706, 746
FauI CCCGC 1 cut(s) 613
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 4 cut(s) 27, 123, 459, 668
FokI GGATG 7 cut(s) 171, 236, 433, 514, 576, 623, 767
Fsp4HI GCNGC 4 cut(s) 27, 123, 459, 668
FspBI CTAG 2 cut(s) 347, 431
GlaI GCGC 1 cut(s) 422
GluI GCNGC 4 cut(s) 27, 123, 459, 668
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 4 cut(s) 71, 78, 584, 762
HapII CCGG 3 cut(s) 66, 108, 759
HhaI GCGC 1 cut(s) 423
Hin1II CATG 6 cut(s) 91, 97, 380, 536, 710, 750
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HinfI GANTC 4 cut(s) 34, 104, 321, 369
HpaII CCGG 3 cut(s) 66, 108, 759
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 85, 491
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 4 cut(s) 108, 152, 509, 533
Hpy8I GTNNAC 2 cut(s) 85, 491
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 571, 612
HpyCH4V TGCA 8 cut(s) 446, 473, 545, 567, 667, 684, 696, 706
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 337, 564, 626
HpyF3I CTNAG 1 cut(s) 301
Hsp92II CATG 6 cut(s) 91, 97, 380, 536, 710, 750
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 107
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 5 cut(s) 57, 263, 293, 651, 733
LmnI GCTCC 3 cut(s) 392, 511, 555
Lsp1109I GCAGC 3 cut(s) 38, 470, 679
LweI GCATC 4 cut(s) 193, 455, 554, 693
MaeI CTAG 2 cut(s) 347, 431
MaeIII GTNAC 1 cut(s) 775
MalI GATC 5 cut(s) 59, 265, 295, 653, 735
MboI GATC 5 cut(s) 57, 263, 293, 651, 733
MflI RGATCY 2 cut(s) 263, 293
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 2 cut(s) 192, 287
MluNI TGGCCA 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 225, 541
MnlI CCTC 7 cut(s) 40, 124, 156, 210, 214, 254, 606
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 708
MroI TCCGGA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 584
MseI TTAA 5 cut(s) 233, 387, 426, 743, 820
MslI CAYNNNNRTG 2 cut(s) 92, 745
Msp20I TGGCCA 1 cut(s) 584
MspI CCGG 3 cut(s) 66, 108, 759
MspR9I CCNGG 2 cut(s) 67, 258
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 4 cut(s) 131, 337, 564, 626
NciI CCSGG 1 cut(s) 67
NdeII GATC 5 cut(s) 57, 263, 293, 651, 733
NlaIII CATG 6 cut(s) 91, 97, 380, 536, 710, 750
NlaIV GGNNCC 6 cut(s) 64, 70, 211, 265, 507, 605
NsiI ATGCAT 1 cut(s) 708
PagI TCATGA 1 cut(s) 532
PfeI GAWTC 4 cut(s) 34, 104, 321, 369
PkrI GCNGC 4 cut(s) 28, 124, 460, 669
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 6 cut(s) 64, 70, 211, 265, 507, 605
PspPI GGNCC 2 cut(s) 69, 761
PsrI GAACNNNNNNTAC 1 cut(s) 792
PstI CTGCAG 2 cut(s) 547, 669
PstNI CAGNNNCTG 1 cut(s) 308
PsuI RGATCY 2 cut(s) 263, 293
RsaI GTAC 4 cut(s) 147, 255, 490, 810
RsaNI GTAC 4 cut(s) 146, 254, 489, 809
RseI CAYNNNNRTG 2 cut(s) 92, 745
SaqAI TTAA 5 cut(s) 233, 387, 426, 743, 820
SatI GCNGC 4 cut(s) 27, 123, 459, 668
Sau3AI GATC 5 cut(s) 57, 263, 293, 651, 733
Sau96I GGNCC 2 cut(s) 69, 761
ScaI AGTACT 1 cut(s) 810
ScrFI CCNGG 2 cut(s) 67, 258
SetI ASST 7 cut(s) 117, 147, 180, 233, 259, 472, 820
SfaNI GCATC 4 cut(s) 193, 455, 554, 693
SfcI CTRYAG 2 cut(s) 543, 665
SmiMI CAYNNNNRTG 2 cut(s) 92, 745
Sse9I AATT 2 cut(s) 192, 287
SsiI CCGC 2 cut(s) 123, 620
SspI AATATT 1 cut(s) 353
SspMI CTAG 2 cut(s) 347, 431
StyD4I CCNGG 2 cut(s) 65, 256
StyI CCWWGG 2 cut(s) 141, 599
TaaI ACNGT 2 cut(s) 571, 612
TaqI TCGA 2 cut(s) 32, 798
TasI AATT 2 cut(s) 192, 287
TatI WGTACW 1 cut(s) 808
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 4 cut(s) 34, 104, 321, 369
Tru1I TTAA 5 cut(s) 233, 387, 426, 743, 820
Tru9I TTAA 5 cut(s) 233, 387, 426, 743, 820
TscAI CASTG 2 cut(s) 574, 592
TseI GCWGC 3 cut(s) 26, 458, 667
TspDTI ATGAA 5 cut(s) 110, 171, 240, 723, 729
TspRI CASTG 2 cut(s) 574, 592
XapI RAATTY 1 cut(s) 287
XspI CTAG 2 cut(s) 347, 431
ZrmI AGTACT 1 cut(s) 810
Zsp2I ATGCAT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.