RLG00000032796

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000922
Physical Location & Seq
Reverse (-)
19148519 .. 19162700
14182 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032796

Sequence Viewer

Length: 1524 bp
ATGTCTTCAATCCCCAGAGACGAAGCAACCTCGATTCAATGGCGGAAGGGCGATTTGATCGGTTCCGGGGCTTTTGGCCGTGTTTACATGGGCATGAATTCTGAATCCGGAGAGCTTATTGCTGTAAAAGAGGTGTTGGTGTTGGTATTGGAGGGGTTTTCCCACTTGAATCCGGAGAGCTTATTTGCTATCAGAGAGAGATATTTAGGAACCGAGAGGGAGGATGAAACCTTGACAATGCTGTTGGAATATGTACCTGGTGGATCCATACAAGCACTATTGGAGAAATTTGGATCTTTTCTGTTGCTATTCACCAAGCAGTTGTTGCTGGCACTCGAATATTTACACGAAAAAGGAATCATACATGGGGACATTAAGCCTACGAGGGGTACACCATACTGGATGGCTCCTGAAGTCATTTGTGGGACTGGTCATGACTTTTCTGCAGATATATGGAGCGTTGGATGCACCGTGATTGAGATGGCCACTGGAAATCCTCCTTGGAACCAACAGTTTGAAACGGTTGCTACTTTCTTTTATTGTGTAGGGACAAGCAAGTCTCATCCACCAATCCCTGAGCATCTTTCTCCTGCAGCAAGAGATTTTCTGCACGAATGTCTGCAAAATTTACATTTGCTGTTGTCTAATGGTAGGGAACCAACTGTAAGGCCATCCGCATCTGAGTTGCTTAAGCATCCTTTCTTAACTGGGAAAGAAGTTGAATCTCAGAATTCTGCCGTCATGGAAGTAGAAGTGGCATCCTCATTTTGCCAAACAAATTCGGATGAAATTTACATCACGTTGGATGATGATGCAAACTCCGATTTTGTATCCGATCCAGCAAGTGGCGGAACTAGAAAATTGATTGTAGAGGGAGCCATTAAACAAGTGTTTTCAAAAATTTATAAATTCTTTAAGAGGTCGGGAAGAACTACAAGAAATAGATCGGCCAAGGGTGAGTTCAGCTATGCATCTATCATGGTGGCCACAGGCAACTTTTCTGAGAAAAATAAGATCGGACAAGGGGGATTTGGAGCTGTTTATAAGGGAAAATTGGTGACCGGACAAGAAGTGGCTGTGAAAAGGCTTTCAAAATCTTCAAATCAAGGAAAATTGCAGTTTAAGAATGAACTGAGACTCATGCGTGAACTCCAACATAGAAACATTATTCGGATATTAGGATTTTGCATTCATATGTTTCTAACAATATATGGGACAGGTTCATCTAGTGACTTGCTACTAGATTGGAATAAGCGTTTCAACATAATTGAAGGAATTGCTCAAGGATTGGTTTACTTGCACAAATACTCCAGACTGAGAGTAATTCATAGAGATGTAAAAGCTAGTAATGTTCTACTTGATGCACAAATGAATCCCAAAGTTGCTGATTTTGGTTTAGCAAAGATTTGTGCAGATGATGAAGAGACCGCAGAAACTGCGGCAATTTGTGGGACAATGGGTTACATGGCTCCCGAATACCTCATAACTGGCATCCTTTCCGTGAAAACCGATGTCTACAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

508

Amino Acids

56.26

Weight (kDa)

6.26

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 15 - 127 8.4e-13 Protein kinase domain
Pkinase PF00069 129 - 235 1.9e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 132 - 209 4.3e-06 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 333 - 507 5.2e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 336 - 507 5.9e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 906, 1044
AasI GACNNNNNNGTC 1 cut(s) 556
AccB7I CCANNNNNTGG 1 cut(s) 845
AccI GTMKAC 1 cut(s) 1515
AccIII TCCGGA 2 cut(s) 107, 172
AciI CCGC 5 cut(s) 43, 675, 849, 1428, 1439
AclWI GGATC 4 cut(s) 258, 271, 301, 830
AcoI YGGCCR 4 cut(s) 76, 483, 948, 984
AcsI RAATTY 8 cut(s) 97, 287, 625, 730, 778, 789, 900, 908
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 255, 391
AfiI CCNNNNNNNGG 2 cut(s) 386, 845
AflII CTTAAG 1 cut(s) 689
AjnI CCWGG 1 cut(s) 256
AjuI GAANNNNNNNTTGG 4 cut(s) 484, 516, 944, 976
AluBI AGCT 5 cut(s) 115, 180, 966, 1037, 1343
AluI AGCT 5 cut(s) 115, 180, 966, 1037, 1343
Alw26I GTCTC 4 cut(s) 12, 564, 1129, 1418
AlwI GGATC 4 cut(s) 258, 271, 301, 830
AlwNI CAGNNNCTG 1 cut(s) 1436
Aor13HI TCCGGA 2 cut(s) 107, 172
AoxI GGCC 5 cut(s) 76, 483, 668, 948, 984
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 8 cut(s) 97, 287, 625, 730, 778, 789, 900, 908
AsuC2I CCSGG 1 cut(s) 67
AsuHPI GGTGA 3 cut(s) 304, 968, 1069
BalI TGGCCA 2 cut(s) 485, 986
BamHI GGATCC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 605
BccI CCATC 3 cut(s) 397, 475, 679
BceAI ACGGC 2 cut(s) 63, 722
BciT130I CCWGG 1 cut(s) 258
BciVI GTATCC 1 cut(s) 841
BcnI CCSGG 1 cut(s) 67
BcoDI GTCTC 4 cut(s) 12, 564, 1129, 1418
BfaI CTAG 4 cut(s) 855, 1227, 1241, 1344
BfmI CTRYAG 3 cut(s) 444, 591, 1516
BfrI CTTAAG 1 cut(s) 689
BfuI GTATCC 1 cut(s) 841
BisI GCNGC 2 cut(s) 594, 1440
BlsI GCNGC 2 cut(s) 595, 1441
Bme1390I CCNGG 2 cut(s) 67, 258
BmiI GGNNCC 8 cut(s) 64, 211, 265, 408, 506, 657, 877, 1470
BmrFI CCNGG 2 cut(s) 67, 258
BmrI ACTGGG 1 cut(s) 717
BmsI GCATC 9 cut(s) 455, 589, 686, 703, 767, 802, 980, 1351, 1500
BmuI ACTGGG 1 cut(s) 717
BpmI CTGGAG 1 cut(s) 1294
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 1266
BpuMI CCSGG 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 1418
BsaJI CCNNGG 3 cut(s) 66, 500, 951
BsaWI WCCGGW 3 cut(s) 107, 172, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 1292, 1322
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 845
Bse1I ACTGG 5 cut(s) 404, 433, 493, 712, 1492
BseAI TCCGGA 2 cut(s) 107, 172
BseBI CCWGG 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 66, 500, 951
BseLI CCNNNNNNNGG 2 cut(s) 386, 845
BseMII CTCAG 6 cut(s) 567, 672, 740, 993, 1124, 1307
BseNI ACTGG 5 cut(s) 404, 433, 493, 712, 1492
BseXI GCAGC 1 cut(s) 605
BsgI GTGCAG 2 cut(s) 593, 1431
BshFI GGCC 5 cut(s) 78, 485, 670, 950, 986
BsiSI CCGG 4 cut(s) 66, 108, 173, 1062
BslFI GGGAC 5 cut(s) 383, 439, 562, 1228, 1465
BslI CCNNNNNNNGG 2 cut(s) 386, 845
BsmAI GTCTC 4 cut(s) 12, 564, 1129, 1418
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 5 cut(s) 383, 439, 562, 1228, 1465
BsmI GAATGC 1 cut(s) 1188
BsnI GGCC 5 cut(s) 78, 485, 670, 950, 986
Bso31I GGTCTC 1 cut(s) 1418
Bsp13I TCCGGA 2 cut(s) 107, 172
Bsp143I GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
BspACI CCGC 5 cut(s) 43, 675, 849, 1428, 1439
BspANI GGCC 5 cut(s) 78, 485, 670, 950, 986
BspCNI CTCAG 6 cut(s) 568, 673, 739, 994, 1125, 1308
BspEI TCCGGA 2 cut(s) 107, 172
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 8 cut(s) 64, 211, 265, 408, 506, 657, 877, 1470
BspMAI CTGCAG 2 cut(s) 448, 595
BspPI GGATC 4 cut(s) 258, 271, 301, 830
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 1418
BsrI ACTGG 5 cut(s) 404, 433, 493, 712, 1492
BssECI CCNNGG 3 cut(s) 66, 500, 951
BssMI GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
BssT1I CCWWGG 2 cut(s) 500, 951
Bst2UI CCWGG 1 cut(s) 258
Bst4CI ACNGT 5 cut(s) 472, 513, 523, 664, 1520
Bst6I CTCTTC 1 cut(s) 1416
BstAFI CTTAAG 1 cut(s) 689
BstAPI GCANNNNNTGC 2 cut(s) 325, 1436
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 6 cut(s) 576, 681, 726, 1002, 1133, 1316
BstEII GGTNACC 1 cut(s) 1057
BstKTI GATC 6 cut(s) 60, 266, 296, 838, 947, 1017
BstMAI GTCTC 4 cut(s) 12, 564, 1129, 1418
BstMBI GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
BstMWI GCNNNNNNNGC 3 cut(s) 325, 465, 1436
BstNI CCWGG 1 cut(s) 258
BstPI GGTNACC 1 cut(s) 1057
BstSCI CCNGG 2 cut(s) 65, 256
BstSFI CTRYAG 3 cut(s) 444, 591, 1516
BstV1I GCAGC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 263, 293
BstYI RGATCY 2 cut(s) 263, 293
BsuI GTATCC 1 cut(s) 841
BsuRI GGCC 5 cut(s) 78, 485, 670, 950, 986
BtsIMutI CAGTG 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 1436
CciI TCATGA 1 cut(s) 433
CsiI ACCWGGT 1 cut(s) 256
Csp6I GTAC 2 cut(s) 254, 390
CviAII CATG 8 cut(s) 88, 94, 365, 434, 742, 979, 1141, 1465
CviQI GTAC 2 cut(s) 254, 390
DdeI CTNAG 6 cut(s) 576, 681, 726, 1002, 1133, 1316
DpnI GATC 6 cut(s) 59, 265, 295, 837, 946, 1016
DpnII GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
DrdI GACNNNNNNGTC 1 cut(s) 556
DseDI GACNNNNNNGTC 1 cut(s) 556
EaeI YGGCCR 4 cut(s) 76, 483, 948, 984
Eam1104I CTCTTC 1 cut(s) 1416
EarI CTCTTC 1 cut(s) 1416
EciI GGCGGA 2 cut(s) 58, 864
Eco130I CCWWGG 2 cut(s) 500, 951
Eco31I GGTCTC 1 cut(s) 1418
Eco57I CTGAAG 1 cut(s) 432
Eco91I GGTNACC 1 cut(s) 1057
EcoO65I GGTNACC 1 cut(s) 1057
EcoRI GAATTC 2 cut(s) 97, 730
EcoRII CCWGG 1 cut(s) 256
EcoT14I CCWWGG 2 cut(s) 500, 951
EcoT22I ATGCAT 1 cut(s) 973
ErhI CCWWGG 2 cut(s) 500, 951
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 8 cut(s) 91, 97, 368, 437, 745, 982, 1144, 1468
FaqI GGGAC 5 cut(s) 383, 439, 562, 1228, 1465
FatI CATG 8 cut(s) 87, 93, 364, 433, 741, 978, 1140, 1464
FauNDI CATATG 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 1515
Fnu4HI GCNGC 2 cut(s) 594, 1440
Fsp4HI GCNGC 2 cut(s) 594, 1440
FspBI CTAG 4 cut(s) 855, 1227, 1241, 1344
GluI GCNGC 2 cut(s) 594, 1440
GsuI CTGGAG 1 cut(s) 1294
HaeIII GGCC 5 cut(s) 78, 485, 670, 950, 986
HapII CCGG 4 cut(s) 66, 108, 173, 1062
Hin1II CATG 8 cut(s) 91, 97, 368, 437, 745, 982, 1144, 1468
HinfI GANTC 7 cut(s) 34, 104, 169, 357, 722, 1137, 1372
HpaII CCGG 4 cut(s) 66, 108, 173, 1062
HphI GGTGA 3 cut(s) 304, 968, 1069
Hpy166II GTNNAC 5 cut(s) 85, 392, 1148, 1294, 1516
Hpy188III TCNNGA 7 cut(s) 108, 173, 410, 434, 924, 1311, 1472
Hpy8I GTNNAC 5 cut(s) 85, 392, 1148, 1294, 1516
HpyAV CCTTC 2 cut(s) 40, 1265
HpyCH4III ACNGT 5 cut(s) 472, 513, 523, 664, 1520
HpyCH4IV ACGT 1 cut(s) 800
HpyF10VI GCNNNNNNNGC 3 cut(s) 325, 465, 1436
HpyF3I CTNAG 6 cut(s) 576, 681, 726, 1002, 1133, 1316
HpySE526I ACGT 1 cut(s) 800
Hsp92II CATG 8 cut(s) 91, 97, 368, 437, 745, 982, 1144, 1468
Kpn2I TCCGGA 2 cut(s) 107, 172
Kzo9I GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
LmnI GCTCC 5 cut(s) 412, 456, 875, 1034, 1474
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 9 cut(s) 455, 589, 686, 703, 767, 802, 980, 1351, 1500
MabI ACCWGGT 1 cut(s) 256
MaeI CTAG 4 cut(s) 855, 1227, 1241, 1344
MaeII ACGT 1 cut(s) 800
MaeIII GTNAC 3 cut(s) 1057, 1229, 1460
MalI GATC 6 cut(s) 59, 265, 295, 837, 946, 1016
MboI GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
MboII GAAGA 3 cut(s) 939, 1089, 1433
MflI RGATCY 2 cut(s) 263, 293
MlsI TGGCCA 2 cut(s) 485, 986
MluNI TGGCCA 2 cut(s) 485, 986
MlyI GAGTC 1 cut(s) 1131
MmeI TCCRAC 4 cut(s) 225, 442, 783, 1177
Mox20I TGGCCA 2 cut(s) 485, 986
Mph1103I ATGCAT 1 cut(s) 973
MroI TCCGGA 2 cut(s) 107, 172
MscI TGGCCA 2 cut(s) 485, 986
MseI TTAA 6 cut(s) 375, 690, 704, 882, 915, 1122
MslI CAYNNNNRTG 3 cut(s) 92, 1193, 1332
Msp20I TGGCCA 2 cut(s) 485, 986
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 4 cut(s) 66, 108, 173, 1062
MspR9I CCNGG 2 cut(s) 67, 258
Mva1269I GAATGC 1 cut(s) 1188
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 3 cut(s) 325, 465, 1436
NciI CCSGG 1 cut(s) 67
NdeI CATATG 1 cut(s) 1194
NdeII GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
NlaIII CATG 8 cut(s) 91, 97, 368, 437, 745, 982, 1144, 1468
NlaIV GGNNCC 8 cut(s) 64, 211, 265, 408, 506, 657, 877, 1470
NmuCI GTSAC 2 cut(s) 1057, 1229
NsiI ATGCAT 1 cut(s) 973
PagI TCATGA 1 cut(s) 433
PctI GAATGC 1 cut(s) 1188
PfeI GAWTC 6 cut(s) 34, 104, 169, 357, 722, 1372
PflMI CCANNNNNTGG 1 cut(s) 845
PkrI GCNGC 2 cut(s) 595, 1441
PleI GAGTC 1 cut(s) 1131
PpsI GAGTC 1 cut(s) 1131
PsiI TTATAA 2 cut(s) 906, 1044
Psp6I CCWGG 1 cut(s) 256
PspEI GGTNACC 1 cut(s) 1057
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 8 cut(s) 64, 211, 265, 408, 506, 657, 877, 1470
PstI CTGCAG 2 cut(s) 448, 595
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 2 cut(s) 263, 293
RsaI GTAC 2 cut(s) 255, 391
RsaNI GTAC 2 cut(s) 254, 390
RseI CAYNNNNRTG 3 cut(s) 92, 1193, 1332
SaqAI TTAA 6 cut(s) 375, 690, 704, 882, 915, 1122
SatI GCNGC 2 cut(s) 594, 1440
Sau3AI GATC 6 cut(s) 57, 263, 293, 835, 944, 1014
SchI GAGTC 1 cut(s) 1131
ScrFI CCNGG 2 cut(s) 67, 258
SexAI ACCWGGT 1 cut(s) 256
SfaNI GCATC 9 cut(s) 455, 589, 686, 703, 767, 802, 980, 1351, 1500
SfcI CTRYAG 3 cut(s) 444, 591, 1516
SmiMI CAYNNNNRTG 3 cut(s) 92, 1193, 1332
SmlI CTYRAG 2 cut(s) 689, 1281
SmoI CTYRAG 2 cut(s) 689, 1281
SsiI CCGC 5 cut(s) 43, 675, 849, 1428, 1439
SspI AATATT 1 cut(s) 341
SspMI CTAG 4 cut(s) 855, 1227, 1241, 1344
StyD4I CCNGG 2 cut(s) 65, 256
StyI CCWWGG 2 cut(s) 500, 951
TaaI ACNGT 5 cut(s) 472, 513, 523, 664, 1520
TaiI ACGT 1 cut(s) 803
TaqI TCGA 2 cut(s) 32, 336
TauI GCSGC 1 cut(s) 1442
TfiI GAWTC 6 cut(s) 34, 104, 169, 357, 722, 1372
Tru1I TTAA 6 cut(s) 375, 690, 704, 882, 915, 1122
Tru9I TTAA 6 cut(s) 375, 690, 704, 882, 915, 1122
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 2 cut(s) 1057, 1229
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 2 cut(s) 1057, 1229
TspDTI ATGAA 9 cut(s) 110, 240, 801, 1143, 1181, 1212, 1316, 1385, 1434
TspGWI ACGGA 1 cut(s) 1489
TspRI CASTG 1 cut(s) 493
Van91I CCANNNNNTGG 1 cut(s) 845
Vha464I CTTAAG 1 cut(s) 689
XapI RAATTY 8 cut(s) 97, 287, 625, 730, 778, 789, 900, 908
XmiI GTMKAC 1 cut(s) 1515
XspI CTAG 4 cut(s) 855, 1227, 1241, 1344
Zsp2I ATGCAT 1 cut(s) 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.