FvH4_3g14790

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000922
Physical Location & Seq
Reverse (-)
9129140 .. 9134423
5284 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14790.t1

Sequence Viewer

Length: 2037 bp
ATGTCATCAGTCGAGTGCAGAGACGAAGCGGCCTCGATCAAATGGCGCAAGGGTGATTTGATCGGGTCCGGGGCTTTTGGCCGTGTTTACATGGCCATGAATACTGAATCCGGAGAGCTTATTGCTGTTAAAGAGGCTCATACCAAGGTGCTTCAAGAAGAAGTGAGGCTTTTAGAAACCCTGTCTCATCCCAAAATTGTTAGATGTTTGGGAATCGAGAGGGAGGAGGAGGATGAAACTTTGACTATGCTGTTGGAATATGTACCCGGTGGATCCATACAATCACTATTGGAGAAATTCGGACCTTTCTCTCTTCCTGTAATAAAAAGATTCACCAAGCAGTTGTTGCTGGCACTAGAATATATACACATAAAAGGAATCATACATGGGGATATTAAGGGAGCAAATATCCTTGTTGGTAGTAAAGGGCGCATTAAACTAGCAGATTTTGGTACATCCAAACAGGCAGCCATTTCAGGTTCAAAGCCTATGAGGGGTACGCCATACTGGATGGCTCCTGAAGTCATTCGTGGGACTGGTCATGACTTCTCTGCGGATATATGGAGCGTTGGATGCACTGTGATTGAGATGGCCACTGGAAATCCTCCGTGGAACCAACAGTTTGAAACGATTGCTGCTTTCTTTTACTGTGTAGGGACAAGCAAGTCTCATCCCCCAATCCCTGAGCATCTTTCTTCTGCAGCAAAAGATTTTCTTCATGAGTGTCTGCAAAAGGAACCAACTGGAAGGCCATCCGCATCTGAGTTGCTTAAGCATCCTTTCTTAACTGGGAAAGAAGCGGAGTGTCAGAATCTTTCTCATTCTGCCGTCATGGAAGTGGAAGTGGCATCCTCATTTTGCCAAACAGATTCTGATGAAACTGATAGCATGTGGGACGACGATGCAAACTCCAATTCTGTATCCAATACAGCTAGTGGCGGAACTAGAAAGTTGATTGAGGAGGAAGCCATTAAACAAGTGTTTACAAAACTTTCTAGATTCTTTAGGCTGTCGAGCAGAATTACAAGAAATAGATCGGAGAAGGGTGAATTCAGCTATGCATCTATCATGGTGGCCACACAGAACTTTTCTGAGGATAAAAAGATCGGACTTGGGGGATTTGGAGCTGTTTATAAGGGGAAATTGGTGACCGGACAAGGCATAGCTGTGAAAAGACTTTCAAGTACATCAAATCAAGGAGATTTGGAGTTTAAGAATGAAGTGAGATTCCTGCGTGAACTCCAACATAGAAACATTATTCAGCTACTAGGATTTTGCATTCATGGAGTCGAAAGAGTTCTAATATACGAGTACATGGACAACGGAAGTTTAAACACACATTTATTTGGTTCATCCAGTGACTTGCTGCTAGATTGGAATAAACGTTTCAACATAATTGAAGGAATTGCTCAAGGATTGGTTTACTTGCACAAATACTCCAGATTGAGAGTAATTCATACAGATATGAAAACTAGCAATATTCTACTTGATGCACAAATGAATCCCAAAATTACTGATTTTGGTATAGCAAAAAGTATTGCAGATGATGAAGAGACAGTAATGACTAATACAATTTGGGGGACACTGGGTTACATGCCTCCTGAGTACCAAATGACGGGCATCTTATCCATGAAAACCGATGTCTACAGTTATGGAGTGTTAATGCTTGAAATCATAAGTGGTAACAAAAACCGCAAAGCTGACGGCAACTATTTGGTAGAAGATGCATGGCAGCTATGGCAAAAAGGTGCAGGGCTAGAACTAGTGGATCCAAAACTTGAAGCTTCTAGTATTAACGAAGATCAATTGTTAAGATGCATCCATGTTGGTCTGCTGTGCACGGAATTAAATGAAGATGATCGGCCGACCATATCAGATGTGATATCTATGTTGACAAATGAAACAGTGCCTTTGCCTAAACCAACAATGCCAGCATTTTCTAGGCCAGCATCTTCTTGCTTCATGAATCTGCCTACTGGAAATGGAGCAGAAACTATGTCCATCAATGATTTATCAATAGAACTAAATACTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04016 ko04722 ko04912 ko05166 map04010 map04016 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

679

Amino Acids

75.2

Weight (kDa)

5.53

Isoelectric Point (pI)

39.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 16 - 262 3.6e-65 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 19 - 258 5.2e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 364 - 627 3.9e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 366 - 629 1.1e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AasI GACNNNNNNGTC 1 cut(s) 664
AccI GTMKAC 1 cut(s) 1644
AccIII TCCGGA 1 cut(s) 110
AciI CCGC 6 cut(s) 29, 554, 756, 800, 939, 1693
AclI AACGTT 1 cut(s) 1384
AclWI GGATC 4 cut(s) 267, 280, 1763, 1776
AcoI YGGCCR 5 cut(s) 79, 93, 591, 1074, 1862
AcsI RAATTY 2 cut(s) 296, 1049
AcuI CTGAAG 1 cut(s) 540
AfaI GTAC 6 cut(s) 264, 454, 499, 1186, 1313, 1607
AfiI CCNNNNNNNGG 2 cut(s) 494, 1615
AflII CTTAAG 1 cut(s) 770
AgsI TTSAA 8 cut(s) 155, 483, 626, 1182, 1390, 1400, 1670, 1781
AhlI ACTAGT 1 cut(s) 1762
AluBI AGCT 9 cut(s) 118, 932, 1056, 1127, 1166, 1264, 1700, 1735, 1784
AluI AGCT 9 cut(s) 118, 932, 1056, 1127, 1166, 1264, 1700, 1735, 1784
Alw21I GWGCWC 1 cut(s) 1841
Alw26I GTCTC 4 cut(s) 15, 189, 672, 1547
Alw44I GTGCAC 1 cut(s) 1837
AlwI GGATC 4 cut(s) 267, 280, 1763, 1776
AlwNI CAGNNNCTG 1 cut(s) 872
Aor13HI TCCGGA 1 cut(s) 110
AoxI GGCC 8 cut(s) 30, 79, 93, 591, 749, 1074, 1862, 1943
ApaLI GTGCAC 1 cut(s) 1837
ApeKI GCWGC 5 cut(s) 467, 635, 701, 1366, 1732
ApoI RAATTY 2 cut(s) 296, 1049
Asp700I GAANNNNTTC 2 cut(s) 525, 1296
AspLEI GCGC 2 cut(s) 48, 432
AspS9I GGNCC 2 cut(s) 66, 302
AsuC2I CCSGG 2 cut(s) 70, 267
AsuHPI GGTGA 4 cut(s) 65, 325, 1058, 1159
AvaII GGWCC 2 cut(s) 66, 302
BaeGI GKGCMC 1 cut(s) 1841
BalI TGGCCA 3 cut(s) 95, 593, 1076
BamHI GGATCC 2 cut(s) 272, 1768
Bbv12I GWGCWC 1 cut(s) 1841
BbvI GCAGC 5 cut(s) 479, 622, 713, 1353, 1744
BccI CCATC 4 cut(s) 505, 583, 760, 2009
BceAI ACGGC 3 cut(s) 66, 812, 1720
BciVI GTATCC 1 cut(s) 931
BcnI CCSGG 2 cut(s) 70, 267
BcoDI GTCTC 4 cut(s) 15, 189, 672, 1547
BcuI ACTAGT 1 cut(s) 1762
BfmI CTRYAG 2 cut(s) 699, 1645
BfrI CTTAAG 1 cut(s) 770
BfuI GTATCC 1 cut(s) 931
BisI GCNGC 6 cut(s) 30, 468, 636, 702, 1367, 1733
BlsI GCNGC 6 cut(s) 31, 469, 637, 703, 1368, 1734
Bme1390I CCNGG 2 cut(s) 70, 267
Bme18I GGWCC 2 cut(s) 66, 302
BmgT120I GGNCC 2 cut(s) 66, 302
BmiI GGNNCC 6 cut(s) 67, 274, 516, 614, 738, 1770
BmrFI CCNGG 2 cut(s) 70, 267
BmrI ACTGGG 2 cut(s) 798, 1595
BmuI ACTGGG 2 cut(s) 798, 1595
BpmI CTGGAG 1 cut(s) 1423
Bpu10I CCTNAGC 1 cut(s) 684
BpuEI CTTGAG 1 cut(s) 1395
BpuMI CCSGG 2 cut(s) 70, 267
BsaJI CCNNGG 3 cut(s) 69, 144, 608
BsaWI WCCGGW 2 cut(s) 110, 1151
BsaXI ACNNNNNCTCC 2 cut(s) 1421, 1451
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 1615
Bse1I ACTGG 8 cut(s) 512, 541, 601, 748, 793, 1356, 1590, 1981
BseAI TCCGGA 1 cut(s) 110
BseDI CCNNGG 3 cut(s) 69, 144, 608
BseLI CCNNNNNNNGG 2 cut(s) 494, 1615
BseMII CTCAG 4 cut(s) 675, 753, 1083, 1593
BseNI ACTGG 8 cut(s) 512, 541, 601, 748, 793, 1356, 1590, 1981
BseRI GAGGAG 3 cut(s) 239, 242, 974
BseSI GKGCMC 1 cut(s) 1841
BseX3I CGGCCG 1 cut(s) 1862
BseXI GCAGC 5 cut(s) 479, 622, 713, 1353, 1744
BsgI GTGCAG 2 cut(s) 37, 1770
Bsh1285I CGRYCG 1 cut(s) 1865
BshFI GGCC 8 cut(s) 32, 81, 95, 593, 751, 1076, 1864, 1945
BsiEI CGRYCG 1 cut(s) 1865
BsiHKAI GWGCWC 1 cut(s) 1841
BsiSI CCGG 4 cut(s) 69, 111, 267, 1152
BslFI GGGAC 4 cut(s) 547, 670, 908, 1594
BslI CCNNNNNNNGG 2 cut(s) 494, 1615
BsmAI GTCTC 4 cut(s) 15, 189, 672, 1547
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 4 cut(s) 547, 670, 908, 1594
BsmI GAATGC 1 cut(s) 1278
BsnI GGCC 8 cut(s) 32, 81, 95, 593, 751, 1076, 1864, 1945
Bsp1286I GDGCHC 1 cut(s) 1841
Bsp13I TCCGGA 1 cut(s) 110
Bsp143I GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
BspACI CCGC 6 cut(s) 29, 554, 756, 800, 939, 1693
BspANI GGCC 8 cut(s) 32, 81, 95, 593, 751, 1076, 1864, 1945
BspCNI CTCAG 4 cut(s) 676, 754, 1084, 1594
BspEI TCCGGA 1 cut(s) 110
BspHI TCATGA 3 cut(s) 541, 718, 1962
BspLI GGNNCC 6 cut(s) 67, 274, 516, 614, 738, 1770
BspMAI CTGCAG 1 cut(s) 703
BspPI GGATC 4 cut(s) 267, 280, 1763, 1776
BspTI CTTAAG 1 cut(s) 770
BsrI ACTGG 8 cut(s) 512, 541, 601, 748, 793, 1356, 1590, 1981
BssECI CCNNGG 3 cut(s) 69, 144, 608
BssMI GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
BssT1I CCWWGG 1 cut(s) 144
Bst4CI ACNGT 7 cut(s) 580, 621, 650, 1558, 1649, 1906, 2032
Bst6I CTCTTC 2 cut(s) 318, 1545
BstAFI CTTAAG 1 cut(s) 770
BstAPI GCANNNNNTGC 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 351, 1932, 1947
BstDEI CTNAG 4 cut(s) 684, 762, 1092, 1602
BstDSI CCRYGG 1 cut(s) 608
BstEII GGTNACC 1 cut(s) 1147
BstHHI GCGC 2 cut(s) 48, 432
BstKTI GATC 8 cut(s) 39, 63, 275, 1037, 1107, 1771, 1804, 1861
BstMAI GTCTC 4 cut(s) 15, 189, 672, 1547
BstMBI GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
BstMCI CGRYCG 1 cut(s) 1865
BstMWI GCNNNNNNNGC 3 cut(s) 346, 573, 1738
BstNSI RCATGY 2 cut(s) 892, 1597
BstPI GGTNACC 1 cut(s) 1147
BstSCI CCNGG 2 cut(s) 68, 265
BstSFI CTRYAG 2 cut(s) 699, 1645
BstSLI GKGCMC 1 cut(s) 1841
BstV1I GCAGC 5 cut(s) 479, 622, 713, 1353, 1744
BstX2I RGATCY 2 cut(s) 272, 1768
BstYI RGATCY 2 cut(s) 272, 1768
BstZI CGGCCG 1 cut(s) 1862
BsuI GTATCC 1 cut(s) 931
BsuRI GGCC 8 cut(s) 32, 81, 95, 593, 751, 1076, 1864, 1945
BtgI CCRYGG 1 cut(s) 608
BtsIMutI CAGTG 5 cut(s) 576, 594, 1363, 1583, 1911
Cac8I GCNNGC 3 cut(s) 351, 1932, 1947
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 3 cut(s) 541, 718, 1962
CfoI GCGC 2 cut(s) 48, 432
Cfr13I GGNCC 2 cut(s) 66, 302
Csp6I GTAC 6 cut(s) 263, 453, 498, 1185, 1312, 1606
CviQI GTAC 6 cut(s) 263, 453, 498, 1185, 1312, 1606
DdeI CTNAG 4 cut(s) 684, 762, 1092, 1602
DpnI GATC 8 cut(s) 38, 62, 274, 1036, 1106, 1770, 1803, 1860
DpnII GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
DraI TTTAAA 1 cut(s) 1332
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 5 cut(s) 79, 93, 591, 1074, 1862
EagI CGGCCG 1 cut(s) 1862
Eam1104I CTCTTC 2 cut(s) 318, 1545
EarI CTCTTC 2 cut(s) 318, 1545
EciI GGCGGA 1 cut(s) 954
EclXI CGGCCG 1 cut(s) 1862
Eco130I CCWWGG 1 cut(s) 144
Eco32I GATATC 1 cut(s) 1884
Eco47I GGWCC 2 cut(s) 66, 302
Eco52I CGGCCG 1 cut(s) 1862
Eco57I CTGAAG 1 cut(s) 540
Eco91I GGTNACC 1 cut(s) 1147
EcoO65I GGTNACC 1 cut(s) 1147
EcoRI GAATTC 1 cut(s) 1049
EcoRV GATATC 1 cut(s) 1884
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 3 cut(s) 1063, 1729, 1820
ErhI CCWWGG 1 cut(s) 144
Esp3I CGTCTC 1 cut(s) 15
FalI AAGNNNNNCTT 4 cut(s) 153, 185, 699, 731
FaqI GGGAC 4 cut(s) 547, 670, 908, 1594
FblI GTMKAC 1 cut(s) 1644
Fnu4HI GCNGC 6 cut(s) 30, 468, 636, 702, 1367, 1733
Fsp4HI GCNGC 6 cut(s) 30, 468, 636, 702, 1367, 1733
GlaI GCGC 2 cut(s) 47, 431
GluI GCNGC 6 cut(s) 30, 468, 636, 702, 1367, 1733
GsuI CTGGAG 1 cut(s) 1423
HaeIII GGCC 8 cut(s) 32, 81, 95, 593, 751, 1076, 1864, 1945
HapII CCGG 4 cut(s) 69, 111, 267, 1152
HhaI GCGC 2 cut(s) 48, 432
Hin6I GCGC 2 cut(s) 46, 430
HinP1I GCGC 2 cut(s) 46, 430
HincII GTYRAC 1 cut(s) 1893
HindII GTYRAC 1 cut(s) 1893
HindIII AAGCTT 1 cut(s) 1782
HpaII CCGG 4 cut(s) 69, 111, 267, 1152
HphI GGTGA 4 cut(s) 65, 325, 1058, 1159
Hpy166II GTNNAC 7 cut(s) 88, 984, 1238, 1423, 1645, 1839, 1893
Hpy188I TCNGA 8 cut(s) 302, 763, 810, 874, 1039, 1093, 1109, 1876
Hpy8I GTNNAC 7 cut(s) 88, 984, 1238, 1423, 1645, 1839, 1893
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 3 cut(s) 741, 1036, 1394
HpyCH4III ACNGT 7 cut(s) 580, 621, 650, 1558, 1649, 1906, 2032
HpyCH4IV ACGT 1 cut(s) 1384
HpyF10VI GCNNNNNNNGC 3 cut(s) 346, 573, 1738
HpyF3I CTNAG 4 cut(s) 684, 762, 1092, 1602
HpySE526I ACGT 1 cut(s) 1384
HspAI GCGC 2 cut(s) 46, 430
Kpn2I TCCGGA 1 cut(s) 110
Kzo9I GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
LmnI GCTCC 5 cut(s) 401, 520, 564, 1124, 1985
Lsp1109I GCAGC 5 cut(s) 479, 622, 713, 1353, 1744
MaeII ACGT 1 cut(s) 1384
MaeIII GTNAC 4 cut(s) 1147, 1358, 1589, 1682
MalI GATC 8 cut(s) 38, 62, 274, 1036, 1106, 1770, 1803, 1860
MboI GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
MboII GAAGA 9 cut(s) 170, 305, 687, 707, 1562, 1733, 1811, 1865, 1944
MfeI CAATTG 1 cut(s) 1805
MflI RGATCY 2 cut(s) 272, 1768
MhlI GDGCHC 1 cut(s) 1841
MlsI TGGCCA 3 cut(s) 95, 593, 1076
MluNI TGGCCA 3 cut(s) 95, 593, 1076
MlyI GAGTC 1 cut(s) 1296
MmeI TCCRAC 3 cut(s) 234, 550, 1267
Mox20I TGGCCA 3 cut(s) 95, 593, 1076
Mph1103I ATGCAT 3 cut(s) 1063, 1729, 1820
MroI TCCGGA 1 cut(s) 110
MroXI GAANNNNTTC 2 cut(s) 525, 1296
MscI TGGCCA 3 cut(s) 95, 593, 1076
MslI CAYNNNNRTG 3 cut(s) 95, 836, 1166
Msp20I TGGCCA 3 cut(s) 95, 593, 1076
MspCI CTTAAG 1 cut(s) 770
MspI CCGG 4 cut(s) 69, 111, 267, 1152
MspR9I CCNGG 2 cut(s) 70, 267
MssI GTTTAAAC 1 cut(s) 1332
MunI CAATTG 1 cut(s) 1805
Mva1269I GAATGC 1 cut(s) 1278
MwoI GCNNNNNNNGC 3 cut(s) 346, 573, 1738
NciI CCSGG 2 cut(s) 70, 267
NdeII GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
NlaIV GGNNCC 6 cut(s) 67, 274, 516, 614, 738, 1770
NmuCI GTSAC 2 cut(s) 1147, 1358
NsiI ATGCAT 3 cut(s) 1063, 1729, 1820
NspI RCATGY 2 cut(s) 892, 1597
PagI TCATGA 3 cut(s) 541, 718, 1962
PctI GAATGC 1 cut(s) 1278
PdmI GAANNNNTTC 2 cut(s) 525, 1296
PkrI GCNGC 6 cut(s) 31, 469, 637, 703, 1368, 1734
PleI GAGTC 1 cut(s) 1295
PmeI GTTTAAAC 1 cut(s) 1332
PpsI GAGTC 1 cut(s) 1295
PsiI TTATAA 1 cut(s) 1134
Psp1406I AACGTT 1 cut(s) 1384
PspEI GGTNACC 1 cut(s) 1147
PspN4I GGNNCC 6 cut(s) 67, 274, 516, 614, 738, 1770
PspPI GGNCC 2 cut(s) 66, 302
PstI CTGCAG 1 cut(s) 703
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 2 cut(s) 272, 1768
RsaI GTAC 6 cut(s) 264, 454, 499, 1186, 1313, 1607
RsaNI GTAC 6 cut(s) 263, 453, 498, 1185, 1312, 1606
RseI CAYNNNNRTG 3 cut(s) 95, 836, 1166
SatI GCNGC 6 cut(s) 30, 468, 636, 702, 1367, 1733
Sau3AI GATC 8 cut(s) 36, 60, 272, 1034, 1104, 1768, 1801, 1858
Sau96I GGNCC 2 cut(s) 66, 302
SchI GAGTC 1 cut(s) 1296
ScrFI CCNGG 2 cut(s) 70, 267
SduI GDGCHC 1 cut(s) 1841
SfcI CTRYAG 2 cut(s) 699, 1645
SinI GGWCC 2 cut(s) 66, 302
SmiMI CAYNNNNRTG 3 cut(s) 95, 836, 1166
SmlI CTYRAG 2 cut(s) 770, 1410
SmoI CTYRAG 2 cut(s) 770, 1410
SpeI ACTAGT 1 cut(s) 1762
SsiI CCGC 6 cut(s) 29, 554, 756, 800, 939, 1693
SspI AATATT 1 cut(s) 1480
StyD4I CCNGG 2 cut(s) 68, 265
StyI CCWWGG 1 cut(s) 144
TaaI ACNGT 7 cut(s) 580, 621, 650, 1558, 1649, 1906, 2032
TaiI ACGT 1 cut(s) 1387
TaqI TCGA 5 cut(s) 12, 35, 216, 1013, 1290
TatI WGTACW 2 cut(s) 1184, 1311
TauI GCSGC 1 cut(s) 32
TscAI CASTG 5 cut(s) 583, 601, 1363, 1590, 1911
TseFI GTSAC 2 cut(s) 1147, 1358
TseI GCWGC 5 cut(s) 467, 635, 701, 1366, 1732
Tsp45I GTSAC 2 cut(s) 1147, 1358
TspGWI ACGGA 3 cut(s) 597, 1338, 1856
TspRI CASTG 5 cut(s) 583, 601, 1363, 1590, 1911
Vha464I CTTAAG 1 cut(s) 770
VneI GTGCAC 1 cut(s) 1837
VpaK11BI GGWCC 2 cut(s) 66, 302
XapI RAATTY 2 cut(s) 296, 1049
XbaI TCTAGA 1 cut(s) 995
XceI RCATGY 2 cut(s) 892, 1597
XmiI GTMKAC 1 cut(s) 1644
XmnI GAANNNNTTC 2 cut(s) 525, 1296
Zsp2I ATGCAT 3 cut(s) 1063, 1729, 1820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.