AT1G03900

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003015
Physical Location & Seq
Forward (+)
991106 .. 993126
2021 bp
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UTR
Exon/CDS
Intron
AT1G03900.1

Sequence Viewer

Length: 819 bp
ATGTCGTTCGAGGAAGAAGAAGAGGAGGAAACATTCGAGCATACACTTCTCGTGGTGCGTGAGGTCTCCGTTTACAAGATCCCGCCGCGAACCACATCCGGCGGATACAAGTGCGGCGAATGGCTTCAATCCGATAAGATCTGGTCTGGTAGACTCCGCGTCGTATCGTGCAAGGATCGATGCGAGATCCGCCTCGAGGATTCGAATTCCGGTGATCTTTTCGCAGCTTGTTTTGTGGATCCTGGACGTAGAGAGAACTCTGTAGAGCCGTCTCTCGATTCGTCTAGGTATTTCGTTCTCAGGATCGATGACGGGAGAGGGAAGTATGCGTTTATCGGGCTAGGATTCGCTGAGAGGAACGAAGCGTTTGATTTCAATGTTGCGTTATCGGATCATGAAAAGTACGTTAGGAGGGAGAAAGAGAAGGAGACAGGGGAAACGAGTGAGAGCGATAATCATATTGATATCCATCCTGCCGTTAATCACAGATTGAAGGAAGGTGAAACCATAAGAATCAACGTGAAGCCGAAACCAACAACAAATGGCACTGGGATGCTCTCAGCTGCTCTTTCAGGAACGGGGAAGCCAAAACCTCTAGCACTTGCCCCACCCCCCAAAGCTGCCGGAGTAACCAGGTCTCCTTTACCTCCTCCTCCAAACGATCCCGTCGCTTCAAGGATTGCATCTGATGGCTGCAAGGAATCACGCAGGAACGAGCCTCTATCTGATCTATCTCAGCTCAAGAAAAATCTTCCTTCAACGGCGGGATCAGGATCGAGCAAGTCAACAGGAGCTGCATCAGGTTGGGCAGCTTTCTGA

Protein Analysis

272

Amino Acids

29.79

Weight (kDa)

6.05

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 12 - 175 4.6e-59 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 194
AccI GTMKAC 1 cut(s) 151
AccII CGCG 2 cut(s) 88, 159
AciI CCGC 7 cut(s) 83, 86, 102, 114, 157, 190, 764
AcsI RAATTY 1 cut(s) 205
AfaI GTAC 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 196
AgsI TTSAA 5 cut(s) 128, 376, 493, 675, 759
AhdI GACNNNNNGTC 1 cut(s) 158
AjnI CCWGG 2 cut(s) 241, 632
AluBI AGCT 6 cut(s) 227, 563, 620, 739, 794, 812
AluI AGCT 6 cut(s) 227, 563, 620, 739, 794, 812
Alw26I GTCTC 4 cut(s) 70, 276, 422, 642
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 1 cut(s) 194
ApeKI GCWGC 6 cut(s) 224, 563, 620, 693, 794, 809
ApoI RAATTY 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 224, 512
AsuII TTCGAA 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 194
BamHI GGATCC 1 cut(s) 238
BauI CACGAG 1 cut(s) 50
BbvI GCAGC 5 cut(s) 236, 550, 607, 680, 781
BccI CCATC 2 cut(s) 477, 683
BceAI ACGGC 3 cut(s) 253, 461, 777
BciT130I CCWGG 2 cut(s) 243, 634
BciVI GTATCC 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 70, 276, 422, 642
BfaI CTAG 3 cut(s) 285, 341, 596
BfmI CTRYAG 1 cut(s) 261
BfuI GTATCC 1 cut(s) 98
BglII AGATCT 1 cut(s) 138
BisI GCNGC 8 cut(s) 86, 115, 225, 564, 621, 694, 795, 810
BlsI GCNGC 8 cut(s) 87, 116, 226, 565, 622, 695, 796, 811
Bme1390I CCNGG 2 cut(s) 243, 634
BmeRI GACNNNNNGTC 1 cut(s) 158
BmeT110I CYCGRG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 2 cut(s) 243, 634
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 4 cut(s) 170, 543, 692, 806
BmuI ACTGGG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 203
BpuEI CTTGAG 1 cut(s) 725
Bsa29I ATCGAT 2 cut(s) 178, 306
BsaBI GATNNNNATC 2 cut(s) 468, 772
BsaI GGTCTC 2 cut(s) 70, 642
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 553
Bse8I GATNNNNATC 2 cut(s) 468, 772
BseBI CCWGG 2 cut(s) 243, 634
BseCI ATCGAT 2 cut(s) 178, 306
BseGI GGATG 3 cut(s) 95, 469, 558
BseJI GATNNNNATC 2 cut(s) 468, 772
BseLI CCNNNNNNNGG 1 cut(s) 196
BseMII CTCAG 4 cut(s) 313, 342, 573, 749
BseNI ACTGG 1 cut(s) 553
BseRI GAGGAG 3 cut(s) 38, 639, 642
BseXI GCAGC 5 cut(s) 236, 550, 607, 680, 781
Bsh1236I CGCG 2 cut(s) 88, 159
BshVI ATCGAT 2 cut(s) 178, 306
BsiHKCI CYCGRG 1 cut(s) 194
BsiSI CCGG 3 cut(s) 99, 210, 624
BslI CCNNNNNNNGG 1 cut(s) 196
BsmAI GTCTC 4 cut(s) 70, 276, 422, 642
BsmBI CGTCTC 1 cut(s) 276
Bso31I GGTCTC 2 cut(s) 70, 642
BsoBI CYCGRG 1 cut(s) 194
Bsp119I TTCGAA 1 cut(s) 203
BspACI CCGC 7 cut(s) 83, 86, 102, 114, 157, 190, 764
BspCNI CTCAG 4 cut(s) 312, 343, 572, 748
BspDI ATCGAT 2 cut(s) 178, 306
BspFNI CGCG 2 cut(s) 88, 159
BspHI TCATGA 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 240
BspT104I TTCGAA 1 cut(s) 203
BspTNI GGTCTC 2 cut(s) 70, 642
BsrI ACTGG 1 cut(s) 553
BssSI CACGAG 1 cut(s) 50
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 243, 634
Bst6I CTCTTC 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 203
BstDEI CTNAG 4 cut(s) 299, 351, 559, 735
BstF5I GGATG 3 cut(s) 95, 469, 558
BstFNI CGCG 2 cut(s) 88, 159
BstMAI GTCTC 4 cut(s) 70, 276, 422, 642
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 2 cut(s) 243, 634
BstSCI CCNGG 2 cut(s) 241, 632
BstSFI CTRYAG 1 cut(s) 261
BstUI CGCG 2 cut(s) 88, 159
BstV1I GCAGC 5 cut(s) 236, 550, 607, 680, 781
BstX2I RGATCY 4 cut(s) 78, 138, 186, 238
BstYI RGATCY 4 cut(s) 78, 138, 186, 238
Bsu15I ATCGAT 2 cut(s) 178, 306
BsuI GTATCC 1 cut(s) 98
BsuTUI ATCGAT 2 cut(s) 178, 306
BtsCI GGATG 3 cut(s) 95, 469, 558
BtsIMutI CAGTG 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 794
CciI TCATGA 1 cut(s) 394
ClaI ATCGAT 2 cut(s) 178, 306
CseI GACGC 1 cut(s) 148
CsiI ACCWGGT 1 cut(s) 632
Csp6I GTAC 1 cut(s) 403
CviAII CATG 1 cut(s) 395
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 4 cut(s) 299, 351, 559, 735
DriI GACNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 1 cut(s) 15
Eam1105I GACNNNNNGTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 2 cut(s) 117, 179
Eco31I GGTCTC 2 cut(s) 70, 642
Eco32I GATATC 1 cut(s) 466
Eco88I CYCGRG 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 205
EcoRII CCWGG 2 cut(s) 241, 632
EcoRV GATATC 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 1 cut(s) 398
FaiI YATR 5 cut(s) 42, 327, 396, 459, 509
FatI CATG 1 cut(s) 394
FauI CCCGC 2 cut(s) 90, 757
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 8 cut(s) 86, 115, 225, 564, 621, 694, 795, 810
FokI GGATG 3 cut(s) 82, 456, 565
Fsp4HI GCNGC 8 cut(s) 86, 115, 225, 564, 621, 694, 795, 810
FspBI CTAG 3 cut(s) 285, 341, 596
GluI GCNGC 8 cut(s) 86, 115, 225, 564, 621, 694, 795, 810
HapII CCGG 3 cut(s) 99, 210, 624
HgaI GACGC 1 cut(s) 148
Hin1II CATG 1 cut(s) 398
HincII GTYRAC 1 cut(s) 786
HindII GTYRAC 1 cut(s) 786
HinfI GANTC 6 cut(s) 153, 200, 278, 345, 513, 701
HpaII CCGG 3 cut(s) 99, 210, 624
HphI GGTGA 2 cut(s) 224, 512
Hpy166II GTNNAC 3 cut(s) 73, 152, 786
Hpy188I TCNGA 5 cut(s) 133, 391, 688, 727, 818
Hpy188III TCNNGA 6 cut(s) 275, 301, 395, 573, 742, 771
Hpy8I GTNNAC 3 cut(s) 73, 152, 786
Hpy99I CGWCG 2 cut(s) 164, 671
HpyAV CCTTC 4 cut(s) 418, 487, 491, 765
HpyCH4IV ACGT 3 cut(s) 247, 405, 519
HpyCH4V TGCA 4 cut(s) 171, 683, 696, 797
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 4 cut(s) 299, 351, 559, 735
HpySE526I ACGT 3 cut(s) 247, 405, 519
Hsp92II CATG 1 cut(s) 398
LmnI GCTCC 1 cut(s) 791
Lsp1109I GCAGC 5 cut(s) 236, 550, 607, 680, 781
LweI GCATC 4 cut(s) 170, 543, 692, 806
MabI ACCWGGT 1 cut(s) 632
MaeI CTAG 3 cut(s) 285, 341, 596
MaeII ACGT 3 cut(s) 247, 405, 519
MaeIII GTNAC 1 cut(s) 628
MboII GAAGA 4 cut(s) 26, 29, 32, 743
MflI RGATCY 4 cut(s) 78, 138, 186, 238
MluCI AATT 1 cut(s) 205
MlyI GAGTC 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 123
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 551
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 3 cut(s) 99, 210, 624
MspR9I CCNGG 2 cut(s) 243, 634
MvaI CCWGG 2 cut(s) 243, 634
MvnI CGCG 2 cut(s) 88, 159
MwoI GCNNNNNNNGC 1 cut(s) 189
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 1 cut(s) 240
NspV TTCGAA 1 cut(s) 203
PaeR7I CTCGAG 1 cut(s) 194
PagI TCATGA 1 cut(s) 394
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 5 cut(s) 200, 278, 345, 513, 701
PfoI TCCNGGA 1 cut(s) 241
PkrI GCNGC 8 cut(s) 87, 116, 226, 565, 622, 695, 796, 811
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 241, 632
PspGI CCWGG 2 cut(s) 241, 632
PspN4I GGNNCC 1 cut(s) 240
PspXI VCTCGAGB 1 cut(s) 194
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 4 cut(s) 78, 138, 186, 238
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
RseI CAYNNNNRTG 1 cut(s) 551
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 8 cut(s) 86, 115, 225, 564, 621, 694, 795, 810
SchI GAGTC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 243, 634
SexAI ACCWGGT 1 cut(s) 632
SfaNI GCATC 4 cut(s) 170, 543, 692, 806
SfcI CTRYAG 1 cut(s) 261
Sfr274I CTCGAG 1 cut(s) 194
SfuI TTCGAA 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 551
SmlI CTYRAG 2 cut(s) 194, 740
SmoI CTYRAG 2 cut(s) 194, 740
Sse9I AATT 1 cut(s) 205
SsiI CCGC 7 cut(s) 83, 86, 102, 114, 157, 190, 764
SspMI CTAG 3 cut(s) 285, 341, 596
StyD4I CCNGG 2 cut(s) 241, 632
TaiI ACGT 3 cut(s) 250, 408, 522
TaqI TCGA 8 cut(s) 9, 36, 178, 195, 203, 276, 306, 776
TasI AATT 1 cut(s) 205
TauI GCSGC 2 cut(s) 88, 117
TfiI GAWTC 5 cut(s) 200, 278, 345, 513, 701
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 1 cut(s) 553
TseI GCWGC 6 cut(s) 224, 563, 620, 693, 794, 809
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 1 cut(s) 553
XapI RAATTY 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 3 cut(s) 285, 341, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.