RLG00000012471

Protein of unknown function (DUF1681)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003015
Physical Location & Seq
Forward (+)
19102435 .. 19103775
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012471

Sequence Viewer

Length: 828 bp
ATGTCGTTCGAGGACGAGGAGGAGTCGTTCGAGCACACGCTGCTGGTGGTGCGCGAGGTCGCCGTCTACAAAATCCCGCCGCGGTCGACGTCCGGCGGGTACAAGTGCGGCGAGTGGCTCCAGTCCGACAAGATCTGGACGGGTCGCCTCCGCGTCGTGTCCTGCAAGGACCGGTGCGAGATCCGGCTCGAGGATCCCAACTCCAGCGAGCTCTTCGCCGCCTGCTTCGTCCCGCCGGGGCAGCGCGAGACCTCCGTCGAGACCGTCCTCGACTCCTCCCGCTACTTCGTCCTCAAGATCGAGGACGGCACCGGCAAGCACGCCTTCATCGGCCTCGGGTTCGCCGAGCGCAACGAGGCCTTCGATTTCAACGTTGCCCTCTCCGATCACGACAAGTACGTCAAGCGAGAGACTGATAATGACGTCGGCGGCGGCGGCGACGACGACAGTCACATTGAGGTTCACCCGGCGGTGAATCACAGATTGAAGGAAGGTGAGACGATAAGGATAAATGTGAAGCCGAAGCCGACGAGTGGGACCGGGATGCTGTCAGCAGCTGGGCTATCCGGGTCGGCAAAGCCCAAATCGCCGGCTTTAAGCCTGGCCCCACCACCCGGGTCCGGGAAACTCAGGTCACCTCTGCCGCCGCCTCCAAATGATCCTGTTGCTGCTAGGATCAGCTCTGGTGGTGTCAAACCTCCGCAGGATAATGCAAGAAAGCGTTCCGACCCGTTATCGGATCTTTCTCAAATTGAGAGGAATCTGCCTCCAGCAAATGGATCAGGATCATCCACAAAGACAAATGCAGTAGGATGGGCAGCTTTCTGA

Protein Analysis

276

Amino Acids

29.65

Weight (kDa)

5.92

Isoelectric Point (pI)

52.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1681 PF07933 10 - 173 1.2e-57 Protein of unknown function (DUF1681)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 398
AatII GACGTC 2 cut(s) 92, 426
AccB1I GGYRCC 1 cut(s) 308
AccB7I CCANNNNNTGG 1 cut(s) 776
AccI GTMKAC 2 cut(s) 66, 86
AccII CGCG 4 cut(s) 54, 82, 153, 246
AclI AACGTT 1 cut(s) 372
AclWI GGATC 8 cut(s) 175, 188, 201, 653, 683, 747, 787, 793
AcyI GRCGYC 2 cut(s) 89, 423
AfaI GTAC 2 cut(s) 101, 398
AfiI CCNNNNNNNGG 7 cut(s) 190, 533, 614, 620, 621, 736, 776
AgeI ACCGGT 1 cut(s) 171
AgsI TTSAA 2 cut(s) 370, 487
AjnI CCWGG 1 cut(s) 600
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 4 cut(s) 211, 557, 681, 821
AluI AGCT 4 cut(s) 211, 557, 681, 821
Alw21I GWGCWC 2 cut(s) 36, 213
Alw26I GTCTC 4 cut(s) 242, 254, 404, 491
AlwI GGATC 8 cut(s) 175, 188, 201, 653, 683, 747, 787, 793
AlwNI CAGNNNCTG 1 cut(s) 557
Ama87I CYCGRG 3 cut(s) 188, 335, 614
AoxI GGCC 3 cut(s) 331, 357, 603
ApeKI GCWGC 5 cut(s) 40, 241, 554, 668, 818
AsiGI ACCGGT 1 cut(s) 171
Asp700I GAANNNNTTC 1 cut(s) 721
AspLEI GCGC 3 cut(s) 54, 246, 351
AspS9I GGNCC 4 cut(s) 169, 537, 604, 618
AsuC2I CCSGG 7 cut(s) 237, 467, 541, 568, 615, 616, 622
AsuHPI GGTGA 4 cut(s) 455, 484, 506, 627
AvaI CYCGRG 3 cut(s) 188, 335, 614
AvaII GGWCC 3 cut(s) 169, 537, 618
BamHI GGATCC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 308
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 36, 213
BbvI GCAGC 4 cut(s) 27, 253, 566, 655
BccI CCATC 1 cut(s) 807
BceAI ACGGC 2 cut(s) 47, 322
BciT130I CCWGG 1 cut(s) 602
BcnI CCSGG 7 cut(s) 237, 467, 541, 568, 615, 616, 622
BcoDI GTCTC 4 cut(s) 242, 254, 404, 491
BfaI CTAG 1 cut(s) 672
BglII AGATCT 1 cut(s) 132
Bme1390I CCNGG 8 cut(s) 237, 467, 541, 568, 602, 615, 616, 622
Bme18I GGWCC 3 cut(s) 169, 537, 618
BmeT110I CYCGRG 3 cut(s) 188, 335, 614
BmgT120I GGNCC 4 cut(s) 169, 537, 604, 618
BmiI GGNNCC 6 cut(s) 119, 195, 310, 538, 606, 619
BmrFI CCNGG 8 cut(s) 237, 467, 541, 568, 602, 615, 616, 622
BmsI GCATC 1 cut(s) 534
BoxI GACNNNNGTC 2 cut(s) 254, 447
BpmI CTGGAG 3 cut(s) 104, 187, 753
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 7 cut(s) 237, 467, 541, 568, 615, 616, 622
BsaBI GATNNNNATC 1 cut(s) 784
BsaHI GRCGYC 2 cut(s) 89, 423
BsaI GGTCTC 2 cut(s) 242, 254
BsaJI CCNNGG 4 cut(s) 80, 236, 334, 614
BsaWI WCCGGW 1 cut(s) 171
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 7 cut(s) 190, 533, 614, 620, 621, 736, 776
Bse118I RCCGGY 3 cut(s) 171, 311, 589
Bse1I ACTGG 1 cut(s) 121
Bse8I GATNNNNATC 1 cut(s) 784
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 4 cut(s) 80, 236, 334, 614
BseGI GGATG 3 cut(s) 549, 788, 818
BseJI GATNNNNATC 1 cut(s) 784
BseLI CCNNNNNNNGG 7 cut(s) 190, 533, 614, 620, 621, 736, 776
BseMII CTCAG 1 cut(s) 643
BseNI ACTGG 1 cut(s) 121
BseRI GAGGAG 3 cut(s) 32, 35, 265
BseXI GCAGC 4 cut(s) 27, 253, 566, 655
BseYI CCCAGC 1 cut(s) 557
Bsh1236I CGCG 4 cut(s) 54, 82, 153, 246
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 3 cut(s) 333, 359, 605
BshNI GGYRCC 1 cut(s) 308
BshTI ACCGGT 1 cut(s) 171
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 2 cut(s) 36, 213
BsiHKCI CYCGRG 3 cut(s) 188, 335, 614
BslFI GGGAC 2 cut(s) 215, 550
BslI CCNNNNNNNGG 7 cut(s) 190, 533, 614, 620, 621, 736, 776
BsmAI GTCTC 4 cut(s) 242, 254, 404, 491
BsmBI CGTCTC 1 cut(s) 491
BsmFI GGGAC 2 cut(s) 215, 550
BsnI GGCC 3 cut(s) 333, 359, 605
Bso31I GGTCTC 2 cut(s) 242, 254
BsoBI CYCGRG 3 cut(s) 188, 335, 614
Bsp1286I GDGCHC 2 cut(s) 36, 213
BspANI GGCC 3 cut(s) 333, 359, 605
BspCNI CTCAG 1 cut(s) 642
BspFNI CGCG 4 cut(s) 54, 82, 153, 246
BspLI GGNNCC 6 cut(s) 119, 195, 310, 538, 606, 619
BspPI GGATC 8 cut(s) 175, 188, 201, 653, 683, 747, 787, 793
BspQI GCTCTTC 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 308
BspTNI GGTCTC 2 cut(s) 242, 254
BsrFI RCCGGY 3 cut(s) 171, 311, 589
BsrI ACTGG 1 cut(s) 121
BssAI RCCGGY 3 cut(s) 171, 311, 589
BssECI CCNNGG 4 cut(s) 80, 236, 334, 614
BssNI GRCGYC 2 cut(s) 89, 423
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 265, 449
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 2 cut(s) 89, 423
BstAPI GCANNNNNTGC 1 cut(s) 40
BstC8I GCNNGC 5 cut(s) 209, 223, 317, 321, 591
BstDEI CTNAG 1 cut(s) 629
BstDSI CCRYGG 1 cut(s) 80
BstEII GGTNACC 1 cut(s) 633
BstF5I GGATG 3 cut(s) 549, 788, 818
BstFNI CGCG 4 cut(s) 54, 82, 153, 246
BstHHI GCGC 3 cut(s) 54, 246, 351
BstMAI GTCTC 4 cut(s) 242, 254, 404, 491
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 5 cut(s) 40, 49, 241, 435, 586
BstNI CCWGG 1 cut(s) 602
BstPAI GACNNNNGTC 2 cut(s) 254, 447
BstPI GGTNACC 1 cut(s) 633
BstSCI CCNGG 8 cut(s) 235, 465, 539, 566, 600, 613, 614, 620
BstUI CGCG 4 cut(s) 54, 82, 153, 246
BstV1I GCAGC 4 cut(s) 27, 253, 566, 655
BstX2I RGATCY 4 cut(s) 132, 180, 193, 739
BstYI RGATCY 4 cut(s) 132, 180, 193, 739
BsuRI GGCC 3 cut(s) 333, 359, 605
BtgI CCRYGG 1 cut(s) 80
BtsCI GGATG 3 cut(s) 549, 788, 818
Cac8I GCNNGC 5 cut(s) 209, 223, 317, 321, 591
CaiI CAGNNNCTG 1 cut(s) 557
CfoI GCGC 3 cut(s) 54, 246, 351
Cfr10I RCCGGY 3 cut(s) 171, 311, 589
Cfr13I GGNCC 4 cut(s) 169, 537, 604, 618
Cfr42I CCGCGG 1 cut(s) 83
Cfr9I CCCGGG 1 cut(s) 614
CseI GACGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 100, 397
CspAI ACCGGT 1 cut(s) 171
CviQI GTAC 2 cut(s) 100, 397
DdeI CTNAG 1 cut(s) 629
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 211
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 213
Eco31I GGTCTC 2 cut(s) 242, 254
Eco47I GGWCC 3 cut(s) 169, 537, 618
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 3 cut(s) 188, 335, 614
Eco91I GGTNACC 1 cut(s) 633
EcoICRI GAGCTC 1 cut(s) 211
EcoO65I GGTNACC 1 cut(s) 633
EcoRII CCWGG 1 cut(s) 600
EcoT38I GRGCYC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 491
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 2 cut(s) 215, 550
FauI CCCGC 4 cut(s) 84, 89, 240, 287
FblI GTMKAC 2 cut(s) 66, 86
FokI GGATG 2 cut(s) 556, 775
FriOI GRGCYC 1 cut(s) 213
FspBI CTAG 1 cut(s) 672
GlaI GCGC 3 cut(s) 53, 245, 350
GsaI CCCAGC 1 cut(s) 561
GsuI CTGGAG 3 cut(s) 104, 187, 753
HaeIII GGCC 3 cut(s) 333, 359, 605
HgaI GACGC 1 cut(s) 142
HhaI GCGC 3 cut(s) 54, 246, 351
Hin1I GRCGYC 2 cut(s) 89, 423
Hin6I GCGC 3 cut(s) 52, 244, 349
HinP1I GCGC 3 cut(s) 52, 244, 349
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 4 cut(s) 23, 272, 475, 760
HphI GGTGA 4 cut(s) 455, 484, 506, 627
Hpy166II GTNNAC 3 cut(s) 67, 87, 463
Hpy188I TCNGA 5 cut(s) 127, 385, 727, 739, 827
Hpy188III TCNNGA 5 cut(s) 136, 259, 295, 389, 783
Hpy8I GTNNAC 3 cut(s) 67, 87, 463
Hpy99I CGWCG 7 cut(s) 91, 158, 260, 428, 443, 446, 532
HpyAV CCTTC 4 cut(s) 334, 370, 481, 485
HpyCH4III ACNGT 2 cut(s) 265, 449
HpyCH4IV ACGT 4 cut(s) 89, 372, 399, 423
HpyCH4V TGCA 3 cut(s) 165, 713, 806
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 49, 241, 435, 586
HpyF3I CTNAG 1 cut(s) 629
HpySE526I ACGT 4 cut(s) 89, 372, 399, 423
Hsp92I GRCGYC 2 cut(s) 89, 423
HspAI GCGC 3 cut(s) 52, 244, 349
KroI GCCGGC 1 cut(s) 589
KroNI GCCGGC 1 cut(s) 591
KspI CCGCGG 1 cut(s) 83
LguI GCTCTTC 1 cut(s) 218
LmnI GCTCC 1 cut(s) 123
Lsp1109I GCAGC 4 cut(s) 27, 253, 566, 655
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 672
MaeII ACGT 4 cut(s) 89, 372, 399, 423
MaeIII GTNAC 2 cut(s) 449, 633
MboII GAAGA 1 cut(s) 205
MflI RGATCY 4 cut(s) 132, 180, 193, 739
MhlI GDGCHC 2 cut(s) 36, 213
MluCI AATT 1 cut(s) 750
MlyI GAGTC 2 cut(s) 32, 266
MmeI TCCRAC 2 cut(s) 150, 750
MroNI GCCGGC 1 cut(s) 589
MroXI GAANNNNTTC 1 cut(s) 721
MseI TTAA 1 cut(s) 596
MspA1I CMGCKG 2 cut(s) 82, 557
MspR9I CCNGG 8 cut(s) 237, 467, 541, 568, 602, 615, 616, 622
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 4 cut(s) 54, 82, 153, 246
MwoI GCNNNNNNNGC 5 cut(s) 40, 49, 241, 435, 586
NaeI GCCGGC 1 cut(s) 591
NciI CCSGG 7 cut(s) 237, 467, 541, 568, 615, 616, 622
NgoMIV GCCGGC 1 cut(s) 589
NlaIV GGNNCC 6 cut(s) 119, 195, 310, 538, 606, 619
NmeAIII GCCGAG 1 cut(s) 370
NmuCI GTSAC 2 cut(s) 449, 633
PaeR7I CTCGAG 1 cut(s) 188
PceI AGGCCT 1 cut(s) 359
PciSI GCTCTTC 1 cut(s) 218
PcsI WCGNNNNNNNCGW 4 cut(s) 342, 360, 369, 396
PdiI GCCGGC 1 cut(s) 591
PdmI GAANNNNTTC 1 cut(s) 721
PfeI GAWTC 2 cut(s) 475, 760
PflMI CCANNNNNTGG 1 cut(s) 776
PfoI TCCNGGA 1 cut(s) 620
PinAI ACCGGT 1 cut(s) 171
PleI GAGTC 2 cut(s) 31, 266
PpsI GAGTC 2 cut(s) 31, 266
PshAI GACNNNNGTC 2 cut(s) 254, 447
Psp124BI GAGCTC 1 cut(s) 213
Psp1406I AACGTT 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 600
PspEI GGTNACC 1 cut(s) 633
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 6 cut(s) 119, 195, 310, 538, 606, 619
PspPI GGNCC 4 cut(s) 169, 537, 604, 618
PspXI VCTCGAGB 1 cut(s) 188
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 4 cut(s) 132, 180, 193, 739
PvuII CAGCTG 1 cut(s) 557
RsaI GTAC 2 cut(s) 101, 398
RsaNI GTAC 2 cut(s) 100, 397
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 83
SalI GTCGAC 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 218
SaqAI TTAA 1 cut(s) 596
Sau96I GGNCC 4 cut(s) 169, 537, 604, 618
SchI GAGTC 2 cut(s) 32, 266
ScrFI CCNGG 8 cut(s) 237, 467, 541, 568, 602, 615, 616, 622
SduI GDGCHC 2 cut(s) 36, 213
SfaNI GCATC 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 83
SgrBI CCGCGG 1 cut(s) 83
SinI GGWCC 3 cut(s) 169, 537, 618
SlaI CTCGAG 1 cut(s) 188
SmaI CCCGGG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 188, 293
SmoI CTYRAG 2 cut(s) 188, 293
Sse9I AATT 1 cut(s) 750
SseBI AGGCCT 1 cut(s) 359
SspMI CTAG 1 cut(s) 672
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 8 cut(s) 235, 465, 539, 566, 600, 613, 614, 620
TaaI ACNGT 2 cut(s) 265, 449
TaiI ACGT 4 cut(s) 92, 375, 402, 426
TaqI TCGA 8 cut(s) 9, 30, 86, 189, 258, 270, 300, 363
TasI AATT 1 cut(s) 750
TauI GCSGC 8 cut(s) 82, 111, 221, 432, 435, 438, 646, 649
TfiI GAWTC 2 cut(s) 475, 760
Tru1I TTAA 1 cut(s) 596
Tru9I TTAA 1 cut(s) 596
TseFI GTSAC 2 cut(s) 449, 633
TseI GCWGC 5 cut(s) 40, 241, 554, 668, 818
Tsp45I GTSAC 2 cut(s) 449, 633
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 1 cut(s) 244
TspMI CCCGGG 1 cut(s) 614
Van91I CCANNNNNTGG 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 169, 537, 618
XhoI CTCGAG 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 614
XmiI GTMKAC 2 cut(s) 66, 86
XmnI GAANNNNTTC 1 cut(s) 721
XspI CTAG 1 cut(s) 672
ZraI GACGTC 2 cut(s) 90, 424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.